The fluorescent offset printing inks are the popular materials used in security printing. The producing process of offset ink includes two main steps as mixing components and grinding ink. This paper presents the studied results regarding the determination of the optimized conditions of the fluorescent offset ink producing process. Three design factors are evaluated as the varnish content, pigment content, and an enhanced degree of roller pressure. The target of the regression function is the particle size of the fluorescent offset ink. The box-wilson steep ascent method and the first-order mathematical model were applied to design a factorial experiment. The optimal conditions obtained from the surface response are varnish content of 74 % by weight, pigment content of 16 % by weight, and pressure enhance of 20 bar. Additionally, the fluorescent offset ink samples prepared under the optimized conditions meet the standards of offset ink namely viscosity, tack value, particle size, and fluorescent properties.