Officinalis Plants Research Articles

Antioxidant activities of traditional medicinal plant species from the Kars (Turkey)

Fourteen different plant parts consisting of leaves, roots, flowers, and seeds were examined separately of Rumex patientia, Hyoscyamus niger, Melilotus officinalis, Lamium album, and Epilobium angustifolium plants. All of these are naturally growing and used for medicinal purposes in Kars province. Five different methods were used to determine the antioxidant capacity of these herbal products. The methods were as follows: determination of total polyphenol, total flavonoid, DPPH● (free radical), CUPRAC (cupric reducing antioxidant capacity), and FRAP (ferric ıon reducing antioxidant power). The data obtained regarding the antioxidant activity demonstrated that the flower part of the Epilobium angustifolium plant obtained from Kars had the highest antioxidant capacity. It was determined that the seeds of the Hyoscyamus niger plant had the lowest antioxidant capacity.

Compacted crops of medicinal and essential oil crops

The characteristics of the growth and development of medicinal and essential oil plants in mono and compacted crops in open ground were studied. Compacted cropping involves the joint cultivation of two (binary) or more crops in one field. Compared to monospecies crops, this method effectively solves the problems of increasing productivity and plant resistance to stress. Purpose of the work: to establish the allelopathic effect of medicinal and essential oil plants in dense sowing. The experimental methodology included the joint cultivation in open ground of medicinal main crops - spearmint (Menthaspicata L.), lemon balm (Melissaofficinalis L.), garden strawberry (Fragariachiloensis L.), and calendula officinalis (Calendulaofficinalis L.) were chosen as complementary crops.), dill (Anethumgraveolens L.). Compacted crops significantly reduce the intensity of transpiration and stomatal conductivity of the main crops. The intensity of photosynthesis largely depends on the species characteristics of the plants, so strawberry plants in binary crops increased photosynthetic activity, while mint and lemon balm on the contrary, they increased the intensity of photosynthesis during monospecies cultivation. According to the results of the studies, the inhibitory effect of calendula officinalis plants in binary crops with lemon balm, spearmint, and garden strawberries was established. At the same time, the use of dill in binary crops increases the yield of spearmint and lemon balm.

Comparative Transcriptome Analysis and Expression of Genes Associated with Polysaccharide Biosynthesis in Dendrobium officinale Diploid and Tetraploid Plants

Dendrobium officinale Kimura et Migo is a kind of herb with high medicinal, ornamental, and commercial value, and is rich in polysaccharides. Polyploid breeding is an important breeding method for the genome doubling of medicinal species to increase biomass and polysaccharide production. Previous studies have revealed comparative transcriptome analysis and polysaccharide biosynthesis across the growth stages and plant parts, but there have been no studies dissecting such genes and pathways in tetraploid D. officinale. Therefore, this study aimed to unravel the molecular mechanisms of the increase in polysaccharide content in tetraploid D. officinale via the generation of four transcriptomic libraries for protocorm-like bodies and six-month-old seedlings of both diploid and tetraploid D. officinale plants. In this study, a total of 230,786,618 clean reads remained with a total of 34.62 Gb nucleotides generated; 274,403 unigenes were assembled, of which 73.99% were annotated to at least one of the protein databases; and of 17,451 unigenes, 6.35% were annotated to all seven protein databases (NR, NT, KO, Swiss-Prot, FAM, GO, and KOG). Putative genes encoding enzymes related to polysaccharide biosynthetic pathways were determined. RT-qPCR for 11 randomly selected genes involved in polysaccharides indicated consistency with RNA-Seq data and polysaccharide content. The expressions of nine genes were higher in tetraploid than in diploid plants, while the expressions of the other two genes encoding bifunctional enzymes were the opposite. This study has provided a foundation for subsequent works regarding the biosynthetic pathways of metabolites involved in the autoploidy of Dendrobium species in general, and D. officinale in particular.

MICROMERIA CONGESTA VE DİĞER BAZI BİTKİ EKSTRELERİNİN ANTİBAKTERİYEL AKTİVİTELERİNİN ARAŞTIRILMASI

With the Covid-19 pandemic, interest in plants and herbal products has increased day by day. The tendency of people towards natural treatment methods has led to more studies on plants and herbal products. The aim of our study was to determine the essential oils of Micromeria congesta, Rosmarinus officinalis L., Sideritis stricta, Artemisia absinthium and Melissa officinalis, which are used in the treatment of various diseases among the people, to evaluate the in vitro antimicrobial activity against Klebsiella pneumoniae, Escherichia coli, Salmonella Typhimurium, Proteus vulgaris, Staphylococcus aureus isolates and Corynebacterium pseudotuberculosis (ATCC 19410) standard strain. The essential oils of M. congesta and M. officinalis plants examined in our study were found to have antibacterial activity as much as reference antibiotics (Streptomycin, Amoxicillin) by disc diffusion method. It was observed that the essential oil of A. absinthium was effective against C. pseudotuberculosis bacteria, while the essential oils of other plants were not effective against the tested bacteria.

Cornus officinalis with high pressure wine steaming enhanced anti-hepatic fibrosis: Possible through SIRT3-AMPK axis

Cornus officinalis, a medicinal and edible plant known for its liver-nourishing properties, has shown promise in inhibiting the activation of hepatic stellate cells (HSCs), crucial indicators of hepatic fibrosis, especially when processed by high pressure wine steaming (HPWS). Herein, this study aims to investigate the regulatory effects of cornus officinalis, both in its raw and HPWS forms, on inflammation and apoptosis in liver fibrosis and their underlying mechanisms. In vivo liver fibrosis models were established by subcutaneous injection of CCl4, while in vitro HSCs were exposed to transforming growth factor-β (TGF-β). These findings demonstrated that cornus officinalis with HPWS conspicuously ameliorated histopathological injury, reduced the release of proinflammatory factors, and decreased collagen deposition in CCl4-induced rats compared to its raw form. Utilizing ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer (UHPLC-QTOF-MS) combined with network analysis, we identified that the pharmacological effects of the changed components of cornus officinalis before and after HPWS, primarily centered on the adenosine phosphate (AMP)-activated protein kinase (AMPK) pathway. Of note, cornus officinalis activated AMPK and sirtuin 3 (SIRT3), promoting the apoptosis of activated HSCs through the caspase cascade by regulating caspase3, caspase6 and caspase9. small interfering RNA (siRNA) experiments showed that cornus officinalis could regulate AMPK activity and its mediated-apoptosis through SIRT3. In conclusion, cornus officinalis exhibited the ability to reduce inflammation and apoptosis, with the SIRT3-AMPK signaling pathway identified as a potential mechanism underlying the synergistic effect of cornus officinalis with HPWS on anti-liver fibrosis.

Green construction of magnetic MnFe2O4/ZIF-8 nanocomposite utilizing extract of Melissa officinalis plant for the photo-degradation of tetracycline under UV illumination

The current work explores the photocatalytic degradation of tetracycline utilizing ZIF-8 grafted with MnFe2O4 through a facile green approach. The complete degradation was achieved at optimized circumstances (pH = 9, catalyst concentration = 0.5 g/L, contact time = 180 min, and 20 mg/L of tetracycline dose). The photocatalytic decontamination reaction of the as-made catalyst was more adherence to the pseudo-first-order model. Recycling experiments demonstrated the excellent stability of the MnFe2O4/ZIF-8 composite after six regeneration runs. Furthermore, the as-constructed composite can simultaneously eliminate tetracycline, COD, and TOC from real wastewater by 84%, 65%, and 50%, respectively, revealing its robust potential in addressing water contamination concerns.

Anti-nutrient contents and methane reduction potential of medicinal plants from maize stover based diet

Greenhouse gas emissions from Ethiopian agriculture are significantly increasing, with the largest share is from enteric fermentation and manure left on pasture. An investigation was conducted to evaluate the anti-nutrient composition and effect of commonly used medicinal plant extracts on enteric methane emission from fibrous feeds using maize stover as substrate feed. Total phenols, flavonoid, tannin and essential oil contents were analyzed using established standards. Effects of leaf extracts of Acacia nilotica, Azadirachta indica, three varieties of Cymbopogon citratus (Cymbopogon citratus-I, Cymbopogon citratus java and Cymbopogon citratus upper awash), Leucaena leucocephala, Moringa stenopetala, three varieties of Rosmarinus officinalis (Rosmarinus officinalis I, Rosmarinus officinalis II and Rosmarinus officinalis III) and Thyme schimperi, seed of three Coriandrum sativum varieties (Coriandrum sativum Batu, Coriandrum sativum Tulu and Coriandrum sativum Waltai) and root of Echinops kebericho on total gas production, digestibility and methane production of maize stover were investigated at different doses using the standard procedures. The results indicated that leaf extracts of Acacia nilotica had the highest (P < 0.001) total phenolic and total tannin contents. Compared to other evaluated plant species, all varieties of Cymbopogon citratus had the highest (P < 0.001) flavonoid content. Significantly high (P < 0.001) essential oil content was observed in Rosmarinus officinalis II than other varieties of Rosmarinus officinalis and other plant species. Significant reduction (P < 0.001) of methane production was observed with extracts of Cymbopoon citratus java (22.5 % less methane than the control) and thyme schimperi (16.7 % less methane than the control) at dose of 50 mg/kg DM. There was also significant (P < 0.001) interaction effect between plant species and dose rates at 50 mg/kg DM for both plant species. It can be concluded that the use of 50 mg/kg DM of Cymbopoon citratus java and Thyme schimperi extract to maize stover reduced methane production without negatively affecting feed digestibility. Further studies are necessary to examine the storability of the extracts in different time durations and evaluate their effects in vivo with animals.

Pharmacological and Medicinal Important of Plant Phyllanthus Emblica Linn. (Syn.Emblica Officinalis), Indian Gooseberry

Abstract: The plant names phyllanthus emblica linn (Amla) are very precious gift of nature which have a vital role in health care. Nowadays, use of herbal product become most important for humans over the world. This drug is very useful for the treatment of disease. The most important part of this plant (phyllanthus emblica) is fruit. Phyllanthus emblica Linn. (syn. Emblica officinalis), commonly known as Indian gooseberry or amla, belongs to the family Euphorbiaceae. Though all parts of the plant are used for medicinal purposes, the fruits especially are found tremendous pharmacological applications. They are highly nutritious and form an important dietary source of vitamin C, amino acids, and minerals. Emblica Officinalis Plant is reported to have diverse Pharmacological actions like Adaptogenic Activity, Hepatoprotective Activity, Anti-Bacterial Activity, Anti-Hyperlipidemia Activity, Hypolipidemic Activity, Anti-Atherogenic Activity, Immunostimulatory Activity, Anti-oxidant Activity, Anti-Tumor Activity,Anti-Microbial Activity,Chondroprotective Activity, Analgesic Activity, Anti-Inflammatory Activity &amp; AntiDiarreheal Activity. Emblica Officinalis plant is used in the treatments of various ailments. The diverse ailments like cancer, Atherosclerosis, Inflammation, Osteoporosis, Nuerological disorders, Hypertention and other infectious disorders.

Optimisation of Three Essential Oils against Salmonella spp. and Escherichia coli by Mixture Designa.

The aim of this work is to optimise the antibacterial activity of essential oils (EOs) from Eucalyptus camaldulensis (ECEO), Mentha pulegium (MPEO) and Rosmarinus officinalis (ROEO) plants against Salmonella spp. and Escherichia coli. The qualitative antimicrobial effect was assessed using the disc diffusion method, the broth microdilution method was used to determine the minimum inhibitory concentrations (MIC). Polynomial models were created using an augmented centroid simplex mixture design to highlight the synergy of EOs. The results show a significant antibacterial effect of ECEO and MPEO against both bacterial strains, with inhibition zones (IZs) of 13 and 12 mm respectively against E. coli, and 13 and 11 mm against Salmonella spp. The latter strain showed a MIC of 0.625 % (v : v) by the ECEO, while E. coli exhibited a MIC of 0.0781 % (v : v). The binary combinations of essential oils display a synergistic effect, the proportions of the optimum EOs in the mixture giving the lowest MICm were of the order of 50.51 % ECEO and 49.49 % ROEO against Salmonella spp. and around 50 % MPEO and 50 % ECEO against E. coli. These results indicate the effectiveness of binary combinations EOs against resistant bacterial strains and suggest their importance in bacterial infections treatment.

The effect of seeds on the intensive development of &lt;i&gt;Taraxacum officinale&lt;/i&gt; sprouts

In order to improve the sowing qualities of seeds and increase the yield of agricultural crops, the issue of impact on seed material does not lose its relevance at any time. Today, in order to increase crop yields, various processing methods are used, in particular, methods of electrophysical stimulation, protective compounds, pills, etc. A simple method of seed treatment in order to influence the quality of sowing and the future harvest is a primer, the features of the research and the mechanisms of the method have not yet lost interest in science. In recent years, the Taraxacum Officinale plant has been paying special attention in order to prevent environmental and economic problems of our country. In particular, there are many studies on the use of the Taraxacum officinale plant as an object of bioindication of polluted, including urbanized territories. The article discusses the development of seedlings under the influence of various chemical reagents on the seeds of the Taraxacum Officinale plant.

Cultivation of Melilotus officinalis as a source of bioactive compounds in association with soil recovery practices.

Melilotus officinalis is a Leguminosae with relevant applications in medicine and soil recovery. This study reports the application of Melilotus officinalis plants in soil recovery and as a source of bioactive compounds. Plants were cultivated in semiarid soil under four different fertilizer treatments, urban waste compost at 10 t/ha and 20 t/ha, inorganic fertilizer and a control (no fertilizer). Agronomic properties of soil (pH, EC, soil respiration, C content, macro- and microelements) were analyzed before and after treatment. Also, germination, biomass, element contents, and physiological response were evaluated. Metabolite composition of plants was analyzed through Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Results showed a significant enhancement of the soil microbial activity in planted soils amended with compost, though there were no other clear effects on the soil physicochemical and chemical characteristics during the short experimental period. An improvement in M. officinalis germination and growth was observed in soils with compost amendment. Metabolite composition of plants was analyzed through Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Principal Component and Agglomerative Hierarchical Clustering models suggest that there is a clear separation of the metabolome of four groups of plants grown under different soil treatments. The five most important discriminative metabolites (annotated) were oleamide, palmitic acid, stearic acid, 3-hydroxy-cis-5-octenoylcarnitine, and 6-hydroxynon-7- enoylcarnitine. This study provides information on how the metabolome of Melilotus might be altered by fertilizer application in poor soil regions. These metabolome changes might have repercussions for the application of this plant in medicine and pharmacology. The results support the profitability of Melilotus officinalis cultivation for bioactive compounds production in association with soil recovery practices.

Could Zingiber officinale plant be effective against Omicron BA.2.75 of SARS-CoV-2?

Zingiber officinale plant was examined in this study. The chemicals found in this plant were identified using the GC-MS method. The activities of the determined chemical molecules against the SARS-CoV-2 Omicron variant were compared. We focused to determine whether Zingiber officinale plant would be an inhibitor against Omicron of SARS-CoV-2 in silico. As a result of theoretical calculations, Zingiber officinale plant was found to contain many chemicals as a result of GC-MS analysis. These chemicals were detected one by one and their activity values were calculated for the SARS-CoV-2 virus. As a result, molecules with high activity were detected. ADME/T properties were investigated in order to examine the drug properties of molecules with high activity. According to ADME/T results, these five molecules examined are suitable for use in human metabolism as drug molecules.

Sensitivity of Taraxacum officinalis (L.) Weber ex F. H. Wigg Pollen to aerogenic pollution in the conditions of a mega city

Aim. To determine the consequences of the impact of automobile emissions on the environment of the city of Kyiv using the palynological method. Methods. The quality of Taraxacum officinalis (L.) Weber ex F. H. Wigg pollen was determined by the iodine method (Gram staining). All pollen grains without typical signs of morphological structure (that is, all but normally developed) were considered teratomorphic. Results. The effect of aerogenous load on the viability of Taraxacum officinalis plant pollen was studied at test sites of park ecosystems of the city of Kyiv with different traffic intensities and proximity to city highways. It was established that in the conditions of the metropolis under various effects of air pollution, the quality indicators of the pollen of the bioindicator remain high, within 77.79 % (test site No. 1, which is located closest to the highways, where Nox is 2.11 g/s, CH – 2,86 g/s) to 81.77 % (test site No. 2 in Mariinsky Park, which has the best environmental conditions: the mixture of nitrogen oxides is 0.41, which is almost five times less than at test site No. 1 in Peremohy Park). The largest share of teratomorphic pollen (34.98 %) was recorded in T. officinalis plants in Peremogy Park test site No. 1, the smallest (26.86 %) was found in plants growing in Mariinsky Park at test site No. 2. When analyzing the data, it was determined that a high level of inverse correlation has an indicator of the fertility of pollen grains with all investigated components of air pollution (r = from -0.57 – Pb compounds to -0.73 – soot). Conclusions. Therefore, the qualitative indicators of the pollen of the phytoindicator T. officinalis can be recommended for use in the system of ecological monitoring of the environment with different degrees of aerogenic load.

Investigation of antimicrobial and anticancer activity of extracts obtained following UV application to Althaea officinalis L. callus cultures

Althaea officinalis L., a plant of the Malvaceae family, is widely used in alternative medicine. The aim of this study is to cultivate the Althaea officinalis plant under in vitro conditions to create an appropriate callus regeneration protocol and investigate the antimicrobial and anticancer activities of methanol and ethyl acetate extracts of calli after UV-C application. Leaf, petiole, and root parts of A. officinalis plants germinated in a sterile environment were used as explant sources. Explants were cultured on MS medium containing different concentrations of 2,4-D (1, 2 mg/l) and BAP (0.25, 0.50, 0.75 mg/l). The most effective (100%) callus growth and callus weight (516.24±0.48 mg) was observed on petiole explants using MS medium containing 1 mg/l 2,4-D + 0.25 mg/l BAP. Calli obtained from leaf and petiole explants were exposed to UV-C treatment. Extractions of calli were carried out using methanol and ethyl acetate solutions. 1 mg/ml, 5 mg/ml, and 10 mg/ml solutions of methanol and ethyl acetate extracts were prepared and their antimicrobial activity on bacteria was investigated using the disc diffusion method for 7 different gram-positive and 9 different gram-negative bacteria. None of the three extract concentrations used had any antimicrobial activities. The anticancer activities of the extracts on SH-SY5Y human neuroblastoma cells were studied using the WST-1 viability kit. 1000, 500, 250, 125, and 62.5 µg/ml concentrations of ethyl acetate extracts of leaf and petiole calli had anticancer activity.

Phytochemical Investigation and Thin Layer Chromatography of Methanol Extract of Psoralea corylifolia and Emblica officinalis Leaves

The current study focuses on phytochemical screening of Psoralea corylifolia and Emblica officinalis leaf extracts in methanol solvent. The antibacterial compounds found in both leaf extracts of Psoralea corylifolia and Emblica officinalis plants were investigated using phytochemical studies. The extracts contained flavonoids, terpenoids, tannins, alkaloids, saponins, and phenolic chemicals, according to preliminary phytochemical screening. The solvent systems of toluene: ethyl acetate: methanol (24:5:2) and chloroform: ethyl acetate: acetic acid (50:50:1) yielded the most phytochemicals from methanolic extracts of Psoralea corylifolia and Emblica officinalis plants, respectively. On TLC plates, these chemicals were separated, resulting in the discovery of different spots in both leaf extracts. The Rf values of methanol extract of P. corylifolia run under toluene: ethyl acetate: methanol (24:5:2) solvent system was 0.12, 0.19, 0.30, 0.41, 0.53, 0.65, 0.77, 0.84, 0.89, and 0.92, respectively, while Rf values of methanol leaf extract of E. officinalis run under chloroform: ethyl acetate: acetic acid (50:50:1)solvent system was 0.23, 0.31, 0.41, 0.64, 0.76 and 0.88 respectively. The results of the investigation will be used to confirm the proper identification of antibacterial fractions from P. corylifolia and E. officinalis crude plant extracts by GC-MS. The optimum solvent for extracting antibacterial components from P. corylifolia and E. officinalis leaves was methanol.

Effect of Zeolux and Filters Backwash Water (FBWW) on Plant Growth and Yield of Sage (Salvia Officinalis) Plant

&lt;p&gt;Jordan is one of the few countries with constricted water resources, having one of the lowest per capita water levels in the world (Ministry of Agriculture, 2015). Jordan's available water resources per person are predicted to fall from less than 160 m3/capita/year to roughly 90 m3/capita/year by 2025, placing the nation in the acute water shortage category. Massive volumes of filter backwash water (FBWW) are created and wasted in Jordan. The use of FBWW to Sage (Salvia Officinalis) plant germination under controlled conditions with various dilution treatments would reduce water waste while also providing an alternate irrigation water supply. The effects of Zeolux as a soil amendment on the productivity and a variety of agronomic parameters of a Sage (Salvia officinalis) irrigated with fresh and FBWW were also studied. Various clay-zeolux mixtures were tested to achieve this goal. The combination soil mix of 25% zeolux and 75% clay watered with FBWW had the greatest results in terms of plant height, number of branches, fresh weight, dry weight, and root dry weight. The results reveal that the addition of zeolux to clay enhanced the yield and agrarian characteristics of plants cultivated in the soil. The results demonstrate that employing FBWW increased plant growth features greatly, and zeolux increases the favorable impact of FBWW irrigation on plant output. The study's main results will help to preserve valuable drinking water supplies while also increasing the yield of irrigated plants by using treated FBWW for irrigation.&lt;/p&gt;&#x0D;

Selenium nanoparticles based on Amphipterygium glaucum extract with antibacterial, antioxidant, and plant biostimulant properties

BackgroundIn recent years, crop production has expanded due to the variety of commercially available species. This increase in production has led to global competition and the search for biostimulant products that improve crop quality and yield. At the same time, agricultural products that protect against diseases caused by phytopathogenic microorganisms are needed. Thus, the green synthesis of selenium nanoparticles (SeNPs) is a proposal for achieving these needs. In this research, SeNPs were synthesized from methanolic extract of Amphipterygium glaucum leaves, and chemically and biologically characterized.ResultsThe characterization of SeNPs was conducted by ultraviolet–visible spectrophotometry (UV–Vis), scanning electron microscopy (SEM), electron microscopy transmission (TEM), Dynamic Light Scattering (DLS), energy dispersion X-ray spectroscopy (EDX), and infrared spectrophotometry (FTIR) techniques. SeNPs with an average size of 40–60 nm and spherical and needle-shaped morphologies were obtained. The antibacterial activity of SeNPs against Serratia marcescens, Enterobacter cloacae, and Alcaligenes faecalis was evaluated. The results indicate that the methanolic extracts of A. glaucum and SeNPs presented a high antioxidant activity. The biostimulant effect of SeNPs (10, 20, 50, and 100 µM) was evaluated in vinca (Catharanthus roseus), and calendula (Calendula officinalis) plants under greenhouse conditions, and they improved growth parameters such as the height, the fresh and dry weight of roots, stems, and leaves; and the number of flowers of vinca and calendula.ConclusionsThe antibacterial, antioxidant, and biostimulant properties of SeNPs synthesized from A. glaucum extract demonstrated in this study support their use as a promising tool in crop production.Graphical

Impact of chitosan on growth, chemical components and essential oil yield of Lavandula officinalis plants.

Impact of chitosan on growth, chemical components and essential oil yield of Lavandula officinalis plants.

Pharmacological Application of Ginger

The basic four components of the Zingiber officinale plant, often known as ginger, are phenols, alkaloids, mucilage, and volatile oils. However, an aromatic essential oil made mainly of sesquiterpenoids, particularly (-)-Zingiberene with smaller amounts of bisabolene, farnesene, -sesquiphellandrene, phellandrene, cineol, and citral. Recent research has shown that ginger has a number of health advantages, including the reduction of menstrual cramps, immune-boosting qualities, treatment of lymphoma, osteoarthritis, ovarian cancer, radiation exposure, blood sugar and cholesterol problems, cataract prevention, chemotherapy-induced nausea relief, and more.

High light triggers flavonoid and polysaccharide synthesis through DoHY5-dependent signaling in Dendrobium officinale.

Dendrobium officinale is edible and has medicinal and ornamental functions. Polysaccharides and flavonoids, including anthocyanins, are important components of D. officinale that largely determine the nutritional quality and consumer appeal. There is a need to study the molecular mechanisms regulating anthocyanin and polysaccharide biosynthesis to enhance D. officinale quality and its market value. Here, we report that high light (HL) induced the accumulation of polysaccharides, particularly mannose, as well as anthocyanin accumulation, resulting in red stems. Metabolome and transcriptome analyses revealed that most of the flavonoids showed large changes in abundance, and flavonoid and polysaccharide biosynthesis was significantly activated under HL treatment. Interestingly, DoHY5 expression was also highly induced. Biochemical analyses demonstrated that DoHY5 directly binds to the promoters of DoF3H1 (involved in anthocyanin biosynthesis), DoGMPP2, and DoPMT28 (involved in polysaccharide biosynthesis) to activate their expression, thereby promoting anthocyanin and polysaccharide accumulation in D. officinale stems. DoHY5 silencing decreased flavonoid- and polysaccharide-related gene expression and reduced anthocyanin and polysaccharide accumulation, whereas DoHY5 overexpression had the opposite effects. Notably, naturally occurring red-stemmed D. officinale plants similarly have high levels of anthocyanin and polysaccharide accumulation and biosynthesis gene expression. Our results reveal a previously undiscovered role of DoHY5 in co-regulating anthocyanin and polysaccharide biosynthesis under HL conditions, improving our understanding of the mechanisms regulating stem color and determining nutritional quality in D. officinale. Collectively, our results propose a robust and simple strategy for significantly increasing anthocyanin and polysaccharide levels and subsequently improving the nutritional quality of D. officinale.