Anther‐derived haploids are potentially useful in plant physiology and breeding programs. Techniques for largescale production of anther‐derived haploids were evaluated. Anthers were excised from immature floral buds that developed on tobacco (Nicotiana tabucum L.) plants in a greenhouse and in widely differing controlled environments. The anthers were cultured in Erlenmeyer flasks and petri dishes in several physical and chemical environments.Haploid plants were successfully cultured from anthers that developed on plants grown under a wide range of light and temperature environments.Numerous haploids were obtained from anthers cultured on different chemically‐defined nutrient formulations with and without auxin and c tokmin. Plantlets emerged earlier from anthers culturedr at 26 than at 21 C. Light was not essential for embryogenesis during anther culture; however, light was needed to prevent etiolation of the plantlets after emergence from the anthers. The simplest successful anther culture environment consisted of a stack of petri dishes under a plastic bag on an office desk.