Off-resonance Imaging Research Articles

Characterization and compensation of inhomogeneity artifact in spiral hyperpolarized 13 C imaging of the human heart.

This study aimed to investigate the role of regional inhomogeneity in spiral hyperpolarized 13 C image quality and to develop measures to alleviate these effects. Field map correction of hyperpolarized 13 C cardiac imaging using spiral readouts was evaluated in healthy subjects. Spiral readouts with differing duration (26 and 45 ms) but similar resolution were compared with respect to off-resonance performance and image quality. An map-based image correction based on the multifrequency interpolation (MFI) method was implemented and compared to correction using a global frequency shift alone. Estimation of an unknown frequency shift was performed by maximizing a sharpness objective based on the Sobel variance. The apparent full width half at maximum (FWHM) of the myocardial wall on [13 C]bicarbonate was used to estimate blur. Mean myocardial wall FWHM measurements were unchanged with the short readout pre-correction (14.1 ± 2.9 mm) and post-MFI correction (14.1 ± 3.4 mm), but significantly decreased in the long waveform (20.6 ± 6.6 mm uncorrected, 17.7 ± 7.0 corrected, P = .007). Bicarbonate signal-to-noise ratio (SNR) of the images acquired with the long waveform were increased by 1.4 ± 0.3 compared to those acquired with the short waveform (predicted 1.32). Improvement of image quality was observed for all metabolites with correction. -map correction reduced blur and recovered signal from dropouts, particularly along the posterior myocardial wall. The low image SNR of [13 C]bicarbonate can be compensated with longer duration readouts but at the expense of increased artifacts, which can be partially corrected for with the proposed methods.

Multi-color background-free coherent anti-Stokes Raman scattering microscopy using a time-lens source.

We demonstrate a multi-color background-free coherent anti-Stokes Raman scattering (CARS) imaging system, using a robust, all-fiber, low-cost, multi-wavelength time-lens source. The time-lens source generates picosecond pulse trains at three different wavelengths. The first is 1064.3 nm, the second is tunable between 1052 nm and 1055 nm, and the third is tunable between 1040 nm and 1050 nm. When the time-lens source is synchronized with a mode-locked Ti:Sa laser, two of the three wavelengths are used to detect different Raman frequencies for two-color on-resonance imaging, whereas the third wavelength is used to obtain the off-resonance image for nonresonant background subtraction. Mixed poly(methyl methacrylate) (PMMA) and polystyrene (PS) beads are used to demonstrate two-color background-free CARS imaging. The synchronized multi-wavelength time-lens source enables pixel-to-pixel wavelength-switching. We demonstrate simultaneous two-color CARS imaging of CH2 and CH3 stretching vibration modes with real-time background subtraction in ex vivo mouse tissue.

Correcting image blur in spiral, retraced in/out (RIO) acquisitions using a maximized energy objective.

Images acquired with spiral k-space trajectories can suffer from off-resonance image blur. Previous work showed that averaging 2 images acquired with a retraced, in/out (RIO) trajectory self-corrects image blur so long as off-resonant spins accrue less than 1 half-cycle of relative phase over the readout. Practical scenarios frequently exceed this threshold. Here, we derive and characterize a more-robust off-resonance image blur correction method for RIO acquisitions. Phantom and human volunteer data were acquired using a RIO trajectory with readout durations ranging from 4 to 60 ms. The resulting images were deblurred using 3 candidate methods: conventional linear correction of the component images; semiautomatic deblurring of the component images using an established minimized phase objective function; and semiautomatic deblurring of the average of the component images using a maximized energy objective function, derived below. Deblurring errors were estimated relative to images acquired with 4 ms readouts. All 3 methods converged to similar solutions in cases where less than 2 and 4 cycles of phase accrued over the readout in in vivo and phantom images, respectively (<13 ms readout at 3T). Above this threshold, the linear and minimized phase methods introduced several errors. The maximized energy function provided accurate deblurring so long as less than 6 and 10 cycles of phase accrued over the readout in in vivo and phantom images, respectively (<34 ms readout at 3T). The maximized energy objective function can accurately deblur RIO acquisitions over a wide spectrum of off resonance frequencies.

Localizing implanted fiducial markers using undersampled co-RASOR MR imaging

The goal of this work was to use an undersampled, dual-plane centre-out radial sampling acquisition pulse sequence, with off-resonance reception, to localize fiducial markers with reduced acquisition time. Two iterative reconstruction techniques, conjugate gradient CG-SENSE and the variational penalty Total Generalized Variation (TGV), were investigated to minimize the undersampling artifacts in off-resonant radial imaging. Simulations of a field perturber were performed at sub-millimeter resolution and reconstructed to display signal pileups that can be radially compressed towards the geometric centre of the perturber for high contrast visualization, but contrast is non-recoverable as the echo time increases. A cylindrical platinum fiducial marker, placed in a phantom parallel and perpendicular to the B0-field was imaged with a short-TE half-echo readout. Contrast-to-Noise (CNR) between the signal of the fiducial its adjacent surrounding shell and half-maximum area were used to compare reconstruction methods and undersampling factors. For single slice acquisitions centred about the fiducial, each slice can be performed in as little as 2.8s. The total acquisition time to localize the fiducial marker in a phantom was reduced to 73s by undersampling (R=8) 37 axial and 15 coronal slices, effectively encoding 1.4s/slice. The noise present in undersampled images, for both scan planes and fiducial orientations, decreased significantly using TGV and CG-SENSE reconstructions, with TGV displaying better spatial resolution from reduced half-maximum area.

The potential of on- and off-resonant formaldehyde imaging combined with bootstrapping in diesel sprays

Formaldehyde (CH2O) is an important intermediate in spray combustion of diesel-like fuels. Experimentally, its spatial distribution is often assessed by means of planar Laser-Induced Fluorescence (PLIF), using a frequency-tripled Nd:YAG laser for excitation and spectrally-filtered fluorescence detection. Especially in soot-laden spray flames, however, considerable spectral interference cannot be avoided, which renders interpretation of the fluorescence distribution challenging. We introduce a more selective strategy, involving dye laser excitation and background subtraction by means of off-resonant excitation in combination with a bootstrap evaluation of sets of individual events. The latter extracts persistent features out of non-simultaneously recorded snapshots of stochastic events, thereby mitigating possible interpretation issues related to the turbulent nature of the process under study. Following this approach, recurrent patterns can be distinguished from random high-intensity events (allegedly due to turbulent fluctuations). We demonstrate our method on the Engine Combustion Network (ECN) “Spray A”-benchmark, with 15 vol-% and 21 vol-% ambient oxygen; we also present spatially well-delimited CH2O fluorescence distributions, and compare these to simultaneously recorded OH* chemiluminescence intensities.

Nanostructured magnetic nanocomposites as MRI contrast agents.

Magnetic resonance imaging (MRI) has become an integral part of modern clinical imaging due to its non-invasiveness and versatility in providing tissue and organ images with high spatial resolution. With the current MRI advancement, MRI imaging probes with suitable biocompatibility, good colloidal stability, enhanced relaxometric properties and advanced functionalities are highly demanded. As such, MRI contrast agents (CAs) have been an extensive research and development area. In the recent years, different inorganic-based nanoprobes comprising inorganic magnetic nanoparticles (MNPs) with an organic functional coating have been engineered to obtain a suitable contrast enhancement effect. For biomedical applications, the organic functional coating is critical to improve colloidal stability and biocompatibility. Simultaneously, it also provides a building block for generating a higher dimensional secondary structure. In this review, the combinatorial design approach by a self-assembling pre-formed hydrophobic inorganic MNPs core (from non-polar thermolysis synthesis) into various functional organic coatings (e.g. ligands, amphiphilic polymers and graphene oxide) to form water soluble nanocomposites will be discussed. The resultant magnetic ensembles were classified based on their dimensionality, namely, 0-D, 1-D, 2-D and 3-D structures. This classification provides further insight into their subsequent potential use as MRI CAs. Special attention will be dedicated towards the correlation between the spatial distribution and the associated MRI applications, which include (i) coating optimization-induced MR relaxivity enhancement, (ii) aggregation-induced MR relaxivity enhancement, (iii) off-resonance saturation imaging (ORS), (iv) magnetically-induced off-resonance imaging (ORI), (v) dual-modalities MR imaging and (vi) multifunctional nanoprobes.

Controlling spontaneous-emission noise in measurement-based feedback cooling of a Bose–Einstein condensate

Off-resonant optical imaging is a popular method for continuous monitoring of a Bose–Einstein condensate. However, the disturbance caused by scattered photons places a serious limitation on the lifetime of such continuously monitored condensates. In this paper, we demonstrate that a new choice of feedback control can overcome the heating effects of the measurement backaction. In particular, we show that the measurement backaction caused by off-resonant optical imaging is a multi-mode quantum-field effect, as the entire heating process is not seen in single-particle or mean-field models of the system. Simulating such continuously monitored systems is possible with the number-phase Wigner particle filter, which currently gives both the highest precision and largest timescale simulations amongst competing methods. It is a hybrid between the leading techniques for simulating non-equilibrium dynamics in condensates and particle filters for simulating high-dimensional non-Gaussian filters in the field of engineering. The new control scheme will enable long-term continuous measurement and feedback on one of the leading platforms for precision measurement and the simulation of quantum fields, allowing for the possibility of single-shot experiments, adaptive measurements and robust state-preparation and manipulation.

IRON magnetic resonance: a robust technique for angiography providing high blood-to-tissue contrast within the clinically approved dosage of superparamagnetic nanoparticles

Conventional MR angiography (MRA) exploits the property of exogenous contrast agents to shorten the T1 relaxation time. However, undesirable signal from surrounding tissue due to T1 recovery remains. IRON MRA is a promising off-resonance imaging technique, and provides in conjunction with superparamagnetic nanoparticles high intravascular contrast between the blood-pool and background tissue without need for image subtraction while T1 recovery of the surrounding tissue is no longer a problem.

Erratum: Andronesi OC, Mintzopoulos D, Psychogios N, et al. Combined off-resonance imaging and T2 relaxation in the rotating frame for positive contrast MR imaging of infection in a murine burn model. J Magn Reson Imaging 2010;32:1172-1183

In the published version of this article, the authorship was incorrectly listed as follows: Ovidiu C. Andronesi, MD, PhD, Dionyssios Mintzopoulos, PhD, Nikolaos Psychogios, PhD, Meenu Kesarwani, PhD, Jianxin He, PhD, Shingo Yasuhara, MD, George Dai, PhD, Laurence G. Rahme, PhD, Aria A. Tzika, PhD The correct authorship is as follows: Ovidiu C. Andronesi, MD, PhD, Dionyssios Mintzopoulos, PhD, Valeria Righi, PhD, Nikolaos Psychogios, PhD, Meenu Kesarwani, PhD, Jianxin He, PhD, Shingo Yasuhara, MD, George Dai, PhD, Laurence G. Rahme, PhD, Aria A. Tzika, PhD

Steady state free precession magnetization transfer imaging

The formerly proposed concept for magnetization transfer imaging (MTI) using balanced steady-state free precession (SSFP) image acquisitions is in this work extended to nonbalanced protocols. This allows SSFP-based MTI of targets with high susceptibility variation (such as the musculoskeletal system), or at ultra-high magnetic fields (where balanced SSFP suffers from considerable off-resonance related image degradations). In the first part, SSFP-based MTI in human brain is analyzed based on magnetization transfer ratio (MTR) histograms. High correlations are observed among all different SSFP MTI protocols and thereby ensure proper conceptual extension to nonbalanced SSFP. The second part demonstrates SSFP-based MTI allowing fast acquisition of high resolution volumetric MTR data from human brain and cartilage at low (1.5T) to ultra-high (7.0T) magnetic fields.

Noninvasive Detection of Macrophage-Rich Atherosclerotic Plaque in Hyperlipidemic Rabbits Using “Positive Contrast” Magnetic Resonance Imaging

This study was designed to identify macrophage-rich atherosclerotic plaque noninvasively by imaging the tissue uptake of long-circulating superparamagnetic nanoparticles with a positive contrast off-resonance imaging sequence (inversion recovery with ON-resonant water suppression [IRON]). The sudden rupture of macrophage-rich atherosclerotic plaques can trigger the formation of an occlusive thrombus in coronary vessels, resulting in acute myocardial infarction. Therefore, a noninvasive technique that can identify macrophage-rich plaques and thereby assist with risk stratification of patients with atherosclerosis would be of great potential clinical utility. Experiments were conducted on a clinical 3-T magnetic resonance imaging (MRI) scanner in 7 heritable hyperlipidemic and 4 control rabbits. Monocrystalline iron-oxide nanoparticles (MION)-47 were administrated intravenously (2 doses of 250 mumol Fe/kg), and animals underwent serial IRON-MRI before injection of the nanoparticles and serially after 1, 3, and 6 days. After administration of MION-47, a striking signal enhancement was found in areas of plaque only in hyperlipidemic rabbits. The magnitude of enhancement on magnetic resonance images had a high correlation with the number of macrophages determined by histology (p < 0.001) and allowed for the detection of macrophage-rich plaque with high accuracy (area under the curve: 0.92, SE: 0.04, 95% confidence interval: 0.84 to 0.96, p < 0.001). No significant signal enhancement was measured in remote areas without plaque by histology and in control rabbits without atherosclerosis. Using IRON-MRI in conjunction with superparamagnetic nanoparticles is a promising approach for the noninvasive evaluation of macrophage-rich, vulnerable plaques.

Off-resonance positive contrast imaging of a passive endomyocardial catheter in swine

The use of off-resonance methods in interventional MRI may be valuable since active devices that provide positive signal enhancements are currently not approved for human use. This study investigated the capacity of a low flip angle steady-state free precession (FLAPS) method for generating off-resonance positive contrast surrounding a susceptibility-shifted endomyocardial Stiletto catheter in excised swine hearts and in live swine. Consistent with theory, discernable positive contrast surrounding the interventional device was visualized under ex-vivo (CNR of 24 ± 2.1 in the left ventricular (LV) chamber and 18 ± 2.7 in LV myocardium) and in-vivo conditions (CNR of 22 ± 3.9 in aorta, 16 ± 4.1 in the LV chamber and 13 ± 0.9 in LV myocardium). The findings show that off-resonance imaging with the FLAPS method may be used for passive device visualization with positive contrast. Further studies are necessary prior to clinical translation.

Impact of field strength and iron oxide nanoparticle concentration on the linearity and diagnostic accuracy of off‐resonance imaging

Off-resonance imaging (ORI) techniques are being increasingly used to image iron oxide imaging agents such as monocrystalline iron oxide nanoparticles (MION). However, the diagnostic accuracy, linearity, and field dependence of ORI have not been fully characterized. In this study, the sensitivity, specificity, and linearity of ORI were thus examined as a function of both MION concentration and magnetic field strength (4.7 and 14 T). MION phantoms with and without an air interface as well as MION uptake in a mouse model of healing myocardial infarction were imaged. MION-induced resonance shifts were shown to increase linearly with MION concentration. In contrast, the ORI signal/sensitivity was highly non-linear, initially increasing with MION concentration until T2 became comparable to the TE and decreasing thereafter. The specificity of ORI to distinguish MION-induced resonance shifts from on-resonance water was found to decrease with increasing field because of the increased on-resonance water linewidths (15 Hz at 4.7 T versus 45 Hz at 14 T). Large resonance shifts ( approximately 300 Hz) were observed at air interfaces at 4.7 T, both in vitro and in vivo, and led to poor ORI specificity for MION concentrations less than 150 microg Fe/mL. The in vivo ORI sensitivity was sufficient to detect the accumulation of MION in macrophages infiltrating healing myocardial infarcts, but the specificity was limited by non-specific areas of positive contrast at the air/tissue interfaces of the thoracic wall and the descending aorta. Improved specificity and linearity can, however, be expected at lower fields where decreased on-resonance water linewidths, reduced air-induced resonance shifts, and longer T2 relaxation times are observed. The optimal performance of ORI will thus likely be seen at low fields, with moderate MION concentrations and with sequences containing very short TEs.

Investigation of fluid cell resonances in intermittent contact mode atomic force microscopy

In fluid, the probe resonance curve of the atomic force microscope contains several apparent resonance peaks whose origin is not well understood. In this work, the authors focus on identifying the cause of these peaks and finding the optimal imaging conditions for acoustic intermittent contact mode in fluid environment. The authors demonstrate that the peaks are also present in the spectrum of the fluid movement and in that of the shaker piezo. These peaks may or may not coincide with the natural resonance of a probe in liquid, thus it is possible to drive the probes off-resonance. Numerical calculations show the feasibility of off-resonance imaging, but predict much higher imaging force.

Diffraction-contrast imaging of cold atoms

We consider the inverse problem of in-line holography, applied to minimally-destructive imaging of cold atom clouds. Absorption imaging near-resonance provides a simple, but destructive measurement of atom column density. Imaging off resonance greatly reduces heating, and sequential images may be taken. Under the conditions required for off-resonant imaging, the generally-intractable inverse problem may be linearized. A minimally-destructive, quantitative and high-resolution image of the atom cloud column density is then retrieved from a single diffraction pattern.

Experimental Analysis of Soot Formation in Sooting Diffusion Flame by Using Laser-Induced Emissions

Two-dimensional images of OH fluorescence, polycyclic aromatic hydrocarbons (PAHs) fluorescence, and laser-induced incandescence (LII) from soot were measured in a sooting diffusion flame. To obtain an accurate OH fluorescence image, two images were taken with the laser wavelength tuned to (“on”) and away from (“off”) the OH absorption line. An accurate OH fluorescence image was obtained by subtracting the off-resonance image from the on-resonance image. For the PAH fluorescence and LII measurements, temporally resolved measurements were used to obtain the individual images; the LII image was obtained by detecting the LII signal after the PAH fluorescence radiation had stopped and the PAH fluorescence image was obtained by subtracting the LII image from the simultaneous image of PAH fluorescence and LII. Based on the obtained images, the relative location of OH, PAH, and soot in the flame was discussed in detail. To investigate the PAH size distribution in a sooting flame using LIF, an estimation strategy for PAH size is proposed. Emission spectra were measured at several heights in the flame using a spectrograph. Since the emission wavelength of PAH fluorescence shifts toward longer wavelengths with increasing PAH size, the main PAH components in the emission spectra could be estimated. The results suggest that PAH grows and the type of PAH changes as the soot inception region was approached. Near the soot inception region, we estimated that the PAHs, which have over 16 carbon atoms, mainly constituted the emission spectrum.

Off-resonant defocus-contrast imaging of cold atoms.

We demonstrate the retrieval of column-density images of cold atoms, using a noninterferometric phase-recovery technique based on a single off-resonant and defocused intensity image. The quantitative column density is retrieved via Fourier inversion and remains robust with respect to detuning and defocus. The technique offers excellent prospects for simple, nondestructive imaging of atoms in magnetic and optical traps and condensates.

Artifact reduction in true-FISP imaging of the coronary arteries by adjusting imaging frequency.

The presence of resonance frequency offsets often causes artifacts in images acquired with true fast imaging with steady-state precession (true-FISP). One source of resonance offsets is a suboptimal setting of the synthesizer frequency. The goal of this work was to demonstrate that shifting the synthesizer frequency could minimize the off-resonance related image artifacts in true-FISP. A simple scouting method was developed to estimate the optimal synthesizer frequency for the volume of interest (VOI). To improve fat suppression, a similar scouting method was also developed to determine the optimal frequency offset for the fat saturation pulse. Coronary artery imaging was performed in healthy subjects using a 3D true-FISP sequence to validate the effectiveness of the frequency corrections. Substantial reduction in image artifacts and improvement in fat suppression were observed by using the water and fat frequencies estimated by the scouting scans. Frequency shifting is a useful and practical method for improving coronary artery imaging using true-FISP.