This study was conducted in season 2019 to evaluate the efficiency of local cotton seeds variety Ashor were irradiated with three doses; 50, 100 and 150 Gry to induce resistance against three fungal pathogens: Fusarium oxysporum FIR 16, Rhizoctonia solani C2 and Rhizoctonia solani Z in pots. Ten parameters were tested in this study; pre-emergence damping off in seedlings, post-emergence damping off in seedlings, fresh and dry weight of shoot and root, length of each root and shoot, disease infection and disease severity for each pathogen of cotton genotype Ashor. The results of this study showed that all tested doses of 50,100, 150 Gry were varied in their ability to inhibit the growth of F. oxysporum FIR 16. R. solani C2 and R. solani Z. Statistical analysis exhibited significant differences (Pd”0.05) with the highest dose 150 Gry which displayed high effect to reduce pre-emergence damping off in seedling of cotton plant Ashor were post treated with the three fungal pathogens F. oxysporum FIR 16, R. solani C2 and R. solani Z which gave the lowest value of 20, 26.67 and 6.67 %, respectively as in comparison with low dose 50 Gry, which cotton plant was more susceptible to infection with pathogens F. oxysporum FIR 16, R. solani C2 and R. solani Z (66.67, 66.67 and 80 %, respectively). The results of growth plant parameters; fresh weight of shoot and root, dry weight of shoot and root exhibited pathogens R. solani Z + 150 Gry and F. oxysporum FIR 16 + 100 Gry which gave the highest rate (11.59, 7.50 g), (2.54, 2.22 g) and (3.51, 2.31 g), (1.74, 1.19 g) respectively, compared with the treatment of unirradiated cotton seeds plus diseases R. solani Z alone and F. oxysporum FIR 16 alone which gave (1.47, 1.62 g), (0.15, 0.27g) and (0.49, 0.39 g), (0.04, 0.05 g), respectively. The results of assessed the effective of cotton plant to induce resistance against sheath blight and wilt diseases caused by R. solani Z and F. oxysporum FIR 16 revealed high efficiency in reducing disease infection in irradiated treatment with dose 150 Gry and 100 Gry which yielded (33.33 and 50 %, respectively), compared with treatments of pathogens only R. solani Z and F. oxysporum FIR 16 which gave (100 % for all). Irradiated seeds treatment 150 Gry with fungus R. solani Z, as well as cotton seeds treated with dose 100 Gry plus pathogen F. oxysporum FIR 16 exhibited high activity to reduction disease severity (6.67 and 16.67 %, respectively) as compared with pathogenic fungi alone R. solani Z and F. oxysporum FIR 16 (88.33 and 86.67 %) after 60 days of planting.