In this study, we present an analysis of deflections in a Morpho butterfly wing using digital holographic interferometry (DHI). Our methodology revolves around an off-axis lensless Fourier holographic setup, using laser excitation to induce deflections in the object. The implementation of a DHI setup, tailored for rapid monitoring of micro-deformation, is a central aspect of our research. We offer an overview of the theoretical foundations of this technique, complemented by both experimental and numerical results aimed at validating our findings. We designed an optical setup that enhanced both laser illumination and hologram reconstruction for the sample. The experimental findings decisively show that the proposed method is effective for rapid deformation analysis. The deformation of the wing can be measured with micro-meter accuracy thanks to numerical analysis.