Estrone Research Articles

The effect of small doses of progesterone on the uterus of the rat.

It is well known that stimulation, by progesterone, of the formation of a »progestational« endometrium is enhanced by pretreatment with oestrogen. As early as 1928, shortly after the discovery of the corpus luteum hormone, Weichert observed that deciduomata of the traumatized uterus developed only if treatment of the rats with corpus luteum extract had been preceded by the administration of follicular hormone. In 1930, Hisaw & Leonard confirmed these results in rabbits. Shelesnyak (1933) was one of the first to emphasize the importance of an optimal ratio between the doses of both hormones. De Fremery & Geerling (1939) found 0.6 mg. of progesterone daily, preceded by 15 μg. of oestrone or stilboestrol to be the most effective combination. Korenchevsky & Hall (1937) found daily doses of 1 μg. of oestrone followed by 1.5 mg. of progesterone to be even more active than 30 μg. of oestrone followed by 3 mg.

ホルモンと腎機能

M. Ono, a member of our laboratory, has once made public a detailed report on the morphological influences of sex hormones on the kidney of an albino rat, but there is scarcely any systematic study on the functional effect of sex hormones upon the kidney. The present work was attempted to shed light on the effect of sex hormones on the renal function, by measuring GFR and RPF respectively with creatinine clearance and with PAH clearance as to mature male rabbits during 2 weeks of daily administration of various sex hormones in a small dose as well as in a large dose.The results obtained were as follows, compared with the control experiment performed on 9 normal rabbits:(A) Effect of male sex hormones on renal function.(1) Testosterone acetate: GFR showed a considerably great increase (Ca 50-70%) during the administration of this hormone in both small and large doses, while RPF showed a little increase only when given in a small dose.(2) Testosterone propionate: the increase of both GFR and RPF was just as effected as when testosterone acetate was injected, but a degree of the increase was less remarkable in GFR and more conspicuous in RPF than in case of testosterone acetate.(3) Following castration, GFR decreased day by day to as low as -40% on the 28th day, while RPF remained hardly changed throughout the experiment.(B) Effect of female sex hormones on renal function.(1) Follicular hormones: by urinary follicular hormone, in both small and large doses, GFR showed a slight tendency of increasing in one week after the administration of the hormone and returned to a normal value in two weeks. RPF increased in one week after the administration of the hormone in a large dose and in 2 weeks decreased, while, in a small dose administration of the hormone, it showed any significant change neither in one week nor in two weeks. By synthetic follicular hormone, GFR remained scarcely effected in both small and large doses, and RPF decreased slightly more remarkably in cases of a large dose than in the case by urinary follicular hormone, while, in case of a small dose, it showed hardly any change.(2) Corpus luteum hormone: by Oophormin luteum (an extract of corpus luteum of a pig) both GFR and RPF remained unchanged even in a large dose, but the synthetic preparation (progesterone acetate) brought on a slight increase of GFR and RPF in both small and large doses.(C) Effects of male sex hormone on damaged renal function.(1) Testosterone acetate, administered following castration, brought on a remarkable recovery of decreased GFR, while no effect on RPF was observed.(2) GFR and RPF, which had decreased by subcutaneous administration of a sublimate solution, recovered normal by the application of testosterone acetate following.(3) The effects, observed in (1) and (2), occurred in most conspicuous degree on 15th day of daily administration of testosterone acetate, and nothing effective was found already in ca. 15 days after discontinuance of testosterone administration.

The Postoperative Vaginal Smears of Patients with the Uterine Cancer, and the Effects Resulting from the Administration of Follicular Hormones

The Postoperative Vaginal Smears of Patients with the Uterine Cancer, and the Effects Resulting from the Administration of Follicular Hormones

Experimental study of the action of folliculin cocarcinogen combined with croton oil

Female rats have been treated with follicular hormone in association with croton oil. In one case only a malignant ovarian tumour was observed. The hypothesis is suggested that oestrogens may promote the carcinogenesis.

The effects of follicular hormones on the blood protein picture

The effects of follicular hormones on the blood protein picture

Studies on follicular hormones. VI. Quantitative analysis of estrone and estradiol by paper chromatography measuring the area of colored spot.

Studies on follicular hormones. VI. Quantitative analysis of estrone and estradiol by paper chromatography measuring the area of colored spot.

Biosynthesis of estrone and beta-estradiol in the perfused ovary.

Biosynthesis of estrone and beta-estradiol in the perfused ovary.

The Fertility and Hatchability of Estrogen-Fed Pullets and Carry-Over Effects on Their Progeny

THE effects of estrogenic hormones on reproduction and growth in the domestic fowl have not been studied intensively. Koch (1935) fed an I. G. Farben extract of follicular hormone (Unden) and noticed a marked improvement in fertility and hatchability. Ebbell (1940) repeated this work using far greater numbers of birds and found no significant differences in either fertility or hatchability. According to Landauer (1948), Prüfer (1936) also made extensive tests of follicular hormone, failing to find any beneficial effect and Simon (1939) found that follicular hormone improved hatchability in some instances but in the majority of cases had no such effect.Pearl (1916) reported that corpus luteum substance retarded growth when fed to young chicks. Due to the manner in which the corpus luteum substance was prepared, there is some doubt that the residue had steroid components present in appreciable amounts so that probably estrogen and progesterone were not involved. .

Comparative investigations concerning the action of the corpus luteum, the follicular hormone and parathormone on dental and osseous tissues: Girolamo Tullio. Clinica Odontoiatrica 5: 222, 1950

Comparative investigations concerning the action of the corpus luteum, the follicular hormone and parathormone on dental and osseous tissues: Girolamo Tullio. Clinica Odontoiatrica 5: 222, 1950

The Occurrence of Oviform Germ Cells by Hormonal Injection in the Regenerated Testes of a Newt

a) Triturus pyrrhogaster has multiple testis made up of two or more small successive lobes. In each testicular lobe certain wave of germ cell differentiation proceeds in cephalo-caudal direction. The spermatogenesis begins at the cephalic end of the lobe and the more the tubules shift caudally they contain the germ cells of the more advanced stages and its caudal end is occupied by the senescent tissue which had extruded the reproductive products in the preceding breeding cycle. At the cephalic apex of the lobe there remains permanent embryonal tissue which supplies the germ cells in the succeeding breeding season. The last named region is readily distinguishable as a white crest at the apex of the lobe.b) When the male is incompletely castrated the tissue of white crest regenerates very well. Spermatogenesis performed in the regene-rated testis agrees in general with that in the normal male.c) The white crest of the testicular lobe regenerates equally well, whether grafted in completely castrated male or female. Grafts from the central region of the testis containing only matured spermatozoa degenerate and eventually disappear.d) In a small number of regenerated testes oviform germ cells of various sizes differentiated intermingling with matured spermatozoa.e) When incompletely castrated male was injected with pelanin benzoate, a preparation of crystalline follicular hormone dissolved in sesame oil, differentiation of oviform germ cells occurred in the majority of cases to a considerably high degree. The injection was executed every month over the range of about one year. Some oviform cells appeared singly in the intervening tissue of seminiferous tubules, others within the confines of tubules, but those developed in the broad connective tissue zone of rather abnormally regenerated testes were largest in size as well as in number. In an extreme case about two-thirds of the entire volume of the regenerated testis was packed with oviform cells of various sizes. The seminiferous tubules neighbouring the connective tissue containing oviform cells are more or less retarded in their spermatogenetic activity.

Effects of Testosterone Propionate on Sex Development in Female Ambystoma Larvae

Previous articleNext article No AccessEffects of Testosterone Propionate on Sex Development in Female Ambystoma LarvaeBeatrice MintzBeatrice Mintz Search for more articles by this author PDFPDF PLUS Add to favoritesDownload CitationTrack CitationsPermissionsReprints Share onFacebookTwitterLinkedInRedditEmail SectionsMoreDetailsFiguresReferencesCited by Volume 20, Number 4Oct., 1947 Article DOIhttps://doi.org/10.1086/physzool.20.4.30151968 Views: 4Total views on this site Citations: 14Citations are reported from Crossref Journal History This article was published in Physiological Zoology (1928-1998), which is continued by Physiological and Biochemical Zoology (1999-present). PDF download Crossref reports the following articles citing this article:Dustin S. Siegel, David M. Sever, Robert D. Aldridge The pelvic kidney of male Ambystoma maculatum (Amphibia, urodela, ambystomatidae) with special reference to the sexual collecting ducts, Journal of Morphology 271, no.1212 (Sep 2010): 1422–1439.https://doi.org/10.1002/jmor.10883 両生類の性決定及び性分化, Newsletter of Japan Society for Comparative Endocrinology 2003, no.109109 (Jan 2003): 109_11–109_19.https://doi.org/10.5983/nl2001jsce.2003.109_11E. Romo, M. P. De Miguel, M. I. Arenas, L. Frago, B. Fraile, R. Paniagua Histochemical and quantitative study of the cloacal glands of Triturus marmoratus marmoratus (Amphibia: Salamandridae), Journal of Zoology 239, no.11 (May 2009): 177–186.https://doi.org/10.1111/j.1469-7998.1996.tb05445.xDavid O. Norris, Harriet B. Austin, Amal S. Huazi Induction of cloacal and dermal skin glands of tiger salamander larvae, (Ambystoma tigrinum): Effects of testosterone and prolactin, General and Comparative Endocrinology 73, no.22 (Feb 1989): 194–204.https://doi.org/10.1016/0016-6480(89)90092-0David O. Norris Regulation of Male Gonaducts and Sex Accessory Structures, (Jan 1987): 327–354.https://doi.org/10.1007/978-1-4613-1869-9_11Günter Dörner Sexualhormonabhängige Differenzierungsprozesse der Sexualorgane und des Hypothalamus, (Jan 1972): 97–129.https://doi.org/10.1007/978-3-7091-5603-2_3L. Gallien Comparative activity of sexual steroids and genetic constitution in sexual differentiation of amphibian embryos, General and Comparative Endocrinology 1 (Jan 1962): 346–355.https://doi.org/10.1016/0016-6480(62)90108-9Emil Witschi EMBRYOLOGY OF THE UTERUS: NORMAL AND EXPERIMENTAL, Annals of the New York Academy of Sciences 75, no.2 The Uterus2 The Uterus (Jan 1959): 412–435.https://doi.org/10.1111/j.1749-6632.1959.tb44565.xJoyce A. Bruner, E. Witschi Pluripotentiality of the mesonephric blastema and the mechanism of feminization of male salamanders by androgenic hormones, The Anatomical Record 120, no.11 (Sep 1954): 99–117.https://doi.org/10.1002/ar.1091200108Beatrice Mintz, Louis Gallien Androgen-induced feminization of urodele larvae in the absence of the hypophysis, The Anatomical Record 119, no.44 (Aug 1954): 493–517.https://doi.org/10.1002/ar.1091190407Chih Ye Chang Parabiosis and gonad transplantation in Xenopus laevis Daudin, Journal of Experimental Zoology 123, no.11 (Jun 1953): 1–27.https://doi.org/10.1002/jez.1401230102DONALD PAUL COSTELLO Growth and Development, (Jan 1949): 115–153.https://doi.org/10.1016/B978-1-4832-0000-2.50008-1ROBERT K. BURNS Hormones and the Differentiation of Sex, (Jan 1949): 233–266.https://doi.org/10.1016/B978-1-4832-0000-2.50010-XR. R. Humphrey Reversal of sex in females of genotype WW in the axolotl (Siredon or Ambystoma mexicanum) and its bearing upon the role of the Z chromosomes in the development of the testis, Journal of Experimental Zoology 109, no.22 (Nov 1948): 171–185.https://doi.org/10.1002/jez.1401090202

Effect of Vitamin B Complex on Inactivation of Estrone in Vivo and in Vitro

Effect of Vitamin B Complex on Inactivation of Estrone <i>in Vivo</i> and <i>in Vitro</i>

THE WEANLING GUINEA PIG AS TEST OBJECT FOR ESTROGEN ASSAYS

SINCE ALLEN and Doisy in 1923 published their useful test for “follicular hormone” other more delicate assay methods have been developed. Lyons and Templeton (1936) showed that by instillation of the material to be tested into the vaginal lumen (and hence in immediate contact with the vaginal wall) a much smaller amount of estrogen could be detected. The endpoint in their experiments was frank cornification, as in the Allen-Doisy test, whereas Fluhmann (1933, 1934) originated an even more sensitive test by taking advantage of the mucification of the superficial cells of the vagina in the castrated mouse, a transformation which requires much less estrogen than is needed for cornification. Albrieux (1936) went further when he combined both methods and added a device of his own for assaying blood estrogen. He first dried a column of blood in a collodion “straw,” then inserted a small “pellet” of the dried blood into the vagina of the spayed mouse. Mucification was the endpoint in this test. Markee and Berg (...

Absence of Alteration in the E.E.G. with Stilbesterol and Progesterone

Dusser de Barenne and Gibbs1 in 1942 presented evidence to indicate that the E.E.G. was modified during the menstrual cycle. Gibbs and Reid2 (1942) demonstrated that in the last weeks of pregnancy the electrical activity of the cortex was definitely slowed compared to postpartum E.E.G. records. Since it is well known that in both menstruation and pregnancy marked changes occur in the blood levels of follicular and corpus luteum hormones, it was decided to determine whether E.E.G. changes could be produced by the administration of stilbesterol and progesterone to women in the menopause.In order that neither the changes during the menstrual cycle nor during pregnancy might interfere with the electrical activity of the cortex three women in the menopause were chosen for study. All three were patients in the Boston Psychopathic Hospital suffering from agitated depressions of moderate severity. None of the patients complained of menopausal symptoms.Daily E.E.G.'s were obtained with the aid of a Grass six-chann...

Effect of Follicular Hormone on Anaphylactic Shock.

Conclusion and SummaryA comparison of the control and alpha-estradiol experiments shows that administration of follicular hormone to serum-sensitized female guinea pigs did not enhance their anaphylactic response. This rinding is in distinct contradiction to Schaefer's results.

A Contribution to the Treatment of Amenorrhea1

THE TIME is recalled when, in cases of functional amenorrhea of one or two years' duration, the patient was advised that a period of at least 6 months' treatment would be required before results, favorable or conclusively negative, could be obtained. The availability of potent estrogenic preparations subsequent to the isolation and synthesis of the female sex hormones has brought about an appreciable reduction in the length of treatment. Nonetheless, the method of treatment devised by Kaufmann (1, 2), which is commonly employed with slight variations, lasts 25 days and necessitates, according to Zondek (3), a minimum of 13 injections, 8 of the follicular hormone and 5 of progesterone. It was believed that in order to produce uterine bleeding in amenorrhoic women, the estrogenic hormone had to be administered first, to stimulate complete proliferation of the uterine mucosa, and then the secretory phase could be induced by the administration of corpus luteum hormone.

Effect of Hormones on Contraction of Striated Muscle and oil Choline Esterase Activity

Summary1. Both the acetylcholine and potassium sensitivity of striated muscle and the activity of choline esterase of brain tissue were investigated in the presence of sex hormones and some of the hormones secreted by the hypophysis. 2. The acetylcholine sensitivity of the muscle is somewhat potentiated by the anterior pituitary-like sex hormone (Follutein, Squibb), the follicular hormone (Estrone Suspension, Abbott; Estradiol, Ciba; Theelin, Parke-Davis), the corpus luteum hormone (Progestin, Roche-Organon; Progesterol, Ciba), Testosterone Propionate (Ciba, Schering), the vasopressor and anti-diuretic hormone of the posterior part of hypophysis (Pitressin, Parke-Davis), but not by the oxytocic principle of the posterior part of hypophysis (Pitocin, Parke-Davis). 3. The activity of the choline esterase is somewhat decreased by Follutein, Estrone Suspension, Progestin, Testosterone Propionate, Pitressin, but not by Pitocin. 4. The potassium sensitivity of the muscle is potentiated by Follutein, Estrone Sus...

Contribution to the Treatment of Essential Pruritus and Kraurosis Vulvae

THE HORMONAL treatment of essential pruritus, kraurosis and leukoplakia of the vulva gives a more favorable prospect for alleviating the symptoms and curing the conditions than any known form of therapy. Roentgen-ray therapy in a series of 22 patients with such degenerative disorders resulted in cure in 27 per cent and temporary relief in 23 per cent of the patients, the remaining 50 per cent being refractory to treatment. Estrogenic therapy in a group of 38 patients suffering from pruritus vulvae resulted in cure in 66 per cent of the cases (4). The local use of an ointment containing estrogen was instituted because it appeared that, when injected, only a fraction of the follicular hormone is utilized by the affected vulval tissues and the prolonged parenteral treatment and high dosage was unnecessary. This mode of topical medication was started independent of Zondek's (10) studies along the same lines and at about the same time.

RELATION OF THE B VITAMINS TO OVARIAN FUNCTION DURING EXPERIMENTAL HYPERTHYROIDISM1

IT HAS BEEN well established that a relationship exists between the thyroid gland and the reproductive organs. Reiss and Pereny (1928) found that the threshold response of castrated rats to follicular hormone was raised during hyperthyroidism, and Weichert and Boyd (1933) and van Horn (1933) produced diestrus in normal rats by feeding thyroid gland. Halpem and Hendryson (1935) studied the effect of dinitrophenol on the estrous cycle of rats, but found only a slight increase in the length of the cycle, which they interpreted as being due to the toxicity of the drug. Varied interpretations have been given as to the mechanism by which thyroid feeding affects the estrous cycle, and the problem is complicated by the eifect of the concomitant loss of weight, and by the effect of food intake on the estrous cycle (Drill and Sherwood, 1938; Mulinos et al,1939).

An Enzyme Which Inactivates Estrone.

SummaryThe properties of estrinase, an estrone inactivating enzyme contained in potatoes, are described.