Esophageal Glands Research Articles

Manipulation of Plant Cells by Cyst and Root-Knot Nematode Effectors

A key feature of sedentary plant-parasitic nematodes is the release of effector proteins from their esophageal gland cells through their stylets into host roots. These proteinaceous stylet secretions have been shown to be crucial for successful parasitism by mediating the transition of normal root cells into specialized feeding sites and by negating plant defenses. Recent technical advances of purifying mRNA from esophageal gland cells of plant-parasitic nematodes coupled with emerging sequencing technologies is steadily expanding our knowledge of nematode effector repertoires. Host targets and biological activities of a number of nematode effectors are continuously being reported and, by now, a first picture of the complexity of sedentary nematode parasitism at the molecular level is starting to take shape. In this review, we highlight effector mechanisms that recently have been uncovered by studying the host-pathogen interaction. These mechanisms range from mediating susceptibility of host plants to the actual triggering of defense responses. In particular, we portray and discuss the mechanisms by which nematode effectors modify plant cell walls, negate host defense responses, alter auxin and polyamine signaling, mimic plant molecules, regulate stress signaling, and activate hypersensitive responses. Continuous molecular characterization of newly discovered nematode effectors will be needed to determine how these effectors orchestrate host signaling pathways and biological processes leading to successful parasitism.

Plant parasitic nematode effectors target host defense and nuclear functions to establish feeding cells

Plant parasitic nematodes are microscopic worms, the most damaging species of which have adopted a sedentary lifestyle within their hosts. These obligate endoparasites have a biotrophic relationship with plants, in which they induce the differentiation of root cells into hypertrophied, multinucleate feeding cells (FCs). Effectors synthesized in the esophageal glands of the nematode are injected into the plant cells via the syringe-like stylet and play a key role in manipulating the host machinery. The establishment of specialized FCs requires these effectors to modulate many aspects of plant cell morphogenesis and physiology, including defense responses. This cell reprogramming requires changes to host nuclear processes. Some proteins encoded by parasitism genes target host nuclei. Several of these proteins were immunolocalized within FC nuclei or shown to interact with host nuclear proteins. Comparative genomics and functional analyses are gradually revealing the roles of nematode effectors. We describe here these effectors and their hypothesized roles in the unique feeding behavior of these pests.

A Novel Effector Protein, MJ-NULG1a, Targeted to Giant Cell Nuclei Plays a Role inMeloidogyne javanicaParasitism

Secretory effector proteins expressed within the esophageal glands of root-knot nematodes (Meloidogyne spp.) are thought to play key roles in nematode invasion of host roots and in formation of feeding sites necessary for nematodes to complete their life cycle. In this study, a novel effector protein gene designated as Mj-nulg1a, which is expressed specifically within the dorsal gland of Meloidogyne javanica, was isolated through suppression subtractive hybridization. Southern blotting and BLAST search analyses showed that Mj-nulg1a is unique for Meloidogyne spp. A real-time reverse-transcriptase polymerase chain reaction assay showed that expression of Mj-nulg1a was upregulated in parasitic second-stage juveniles and declined in later parasitic stages. MJ-NULG1a contains two putative nuclear localization signals and, consistently, in planta immunolocalization analysis showed that MJ-NULG1a was localized in the nuclei of giant cells during nematode parasitism. In planta RNA interference targeting Mj-nulg1a suppressed the expression of Mj-nulg1a in nematodes and attenuated parasitism ability of M. javanica. In contrast, transgenic Arabidopsis expressing Mj-nulg1a became more susceptible to M. javanica infection than wild-type control plants. These results depict a novel nematode effector that is targeted to giant cell nuclei and plays a critical role in M. javanica parasitism.

Columnar‐lined esophagus in Chinese patients with proximal gastric carcinomas

To investigate the clinicopathological features of columnar-lined esophagus (CLE) in Chinese patients. Among 182 consecutive resections of proximal gastric cancer with residual esophageal tissue, 114 were eligible for the study. The maximal lengths of CLE and superficial esophageal glands (SEG) and the presence or absence of chronic inflammation (CI), lymphoid follicles (LF), Helicobacter pylori (H. pylori) infection, intestinal metaplasia (IM), pancreatic metaplasia (PM) and dysplasia were analyzed and compared with those in the adjacent proximal gastric mucosa. SEG were present in 110 cases with a mean length of 5.4 mm (range 1-16 mm, 90% equal to or under 10 mm and 10% between 11-16 mm). These glands were associated with CI (95%), LF (85%), H. pylori infection (42%), IM (25%), PM (36%) and dysplasia (10%). CLE was found in 65% of the cases and was associated with SEG (mean length of 4.0 mm, range 1-13 mm, 97% within 10 mm and 3% between 11-13 mm). The frequencies of CI (97%), LF (86%), H. pylori infection (65%) and PM (46%) in CLE were similar to those in the proximal stomach (CI 90%, LF 54%, H. pylori infection 58%, PM 39%). In contrast, the frequencies of IM (37%) and dysplasia (15%) in CLE were significantly lower than those in the proximal stomach (66% and 31%, respectively; P < 0.01). CLE in Chinese patients was within 10 mm in length in 97% of cases, and with SEG and H. pylori infection-related changes similar to those in the proximal stomach.

An Approach to the Parasitism Genes of the Root Knot Nematode

Plant parasitic nematodes which are highly successful parasites evolved a very specialized feeding relationship with the host plant to cause the destructive root-knot disease. They initiate their parasitic relationship with the host by releasing their secretions into root cells which in turn stimulate the root cells of the host to become specialized feeding cells which are considered as the single source of nutrients essential for the nematode's survival. The parasitism genes expressed in nematode's esophageal gland cells encode secretory proteins that are released through its stylet to direct the interactions of the nematode with its host plants.

Proteomic profiles of soluble proteins from the esophageal gland in female Meloidogyne incognita

Meloidogyne incognita can infect multiple plant species. Proteins synthesized in the esophageal glands and secreted through the stylet of plant parasitic nematodes play critical roles in the plant-nematode interactions. Female M. incognita live for approximately 15days, embedded in a host plant, but their esophageal gland proteins have not yet been comprehensively analyzed. In this study, a new bacterium-contamination-resistant method for collecting soluble proteins from esophageal gland cells (SPEGC) of female M. incognita was established. Approximately 5μg of freeze-dried proteins could be extracted from 150 female M. incognita. Bands of a one-dimensional SDS–polyacrylamide gel were excised after electrophoresis of 20μg of protein and were analyzed. Two hundred and forty-six proteins from SPEGC of female M. incognita were identified by LC–MS/MS. Gene Ontology analysis suggests that many of the secreted proteins are involved in protein or carbohydrate metabolism and proteolysis. Some of the SPEGC (46.3%) were predicted to be secreted through classical or non-classical secretory pathways. The described method presents a new approach for the identification of proteins stored in SPEGC of an important plant parasitic nematode. This global proteomic profile of SPEGC provides a basis for future studies to elucidate the functions of proteins secreted from female M. incognita on plant responses.

Gross and histological studies of digestive tract of broilers during postnatal growth and development

We studied anatomy and histology of different segments of the digestive tract in postnatal growing broiler chickens with regard to their location, shape, size and weight. A group of four chickens, each at day 1 (D1), days 14 (D14) and days 28 (D28), total 12, were killed, their digestive tracts were dissected and described and shape, size and weight of different segments were recorded. Samples from different segments were prepared and stained with haematoxylin and eosin staining technique to study the histology under light microscope. The average lengths (cm) and weights (gm) of esophagus, proventriculus (glandular stomach), gizzard (muscular stomach), small intestine and large intestine were significantly higher (P&lt;0.01) in chickens at D28 than that at D14 and at D1. The histological layers of digestive tract were lamina epithelia, lamina propria, lamina muscularis, submucosa, tunica muscularis and serosa with the exception in esophagus where outer adventitia was found. The esophageal glands were significantly more in numbers at D1 (in cross section, 12 in number per focus under 100x) than at D14 (6 in number per focus under 100x) and at D28 (2 in number per focus under 100x). The proventriculus consisted of macroscopic papillae with numerous microscopic folds with lamina propria comprised of simple glands, which converged into a common cavity near the surface. In gizzard, the cuticle, in the form of wavy lines ran parallel to the surface. The villi of small intestine and large intestine were lined by simple columnar epithelium. The apical parts of villi of the duodenum were slightly pointed and the basal parts of the villi were thicker than jejunum and ileum, whereas, the villi of the jejunum and ileum became shorter and broader than duodenum and most of the villi had blunt apical part and the basal parts were wider. The numbers of goblet cells were numerous in number in ileum than duodenum and jejunum. Plicae ran along the inner surface of the distal two thirds of the cacea. However, in the colorectum, the villi appeared as numerous long flat leaf-shaped structures which filled a large proportion of the lumen. The average lengths and widths of villi of small and large intestine were significantly higher (P&lt;0.01) in chickens at D28 than that at D14 and at D1. The number of goblet cells in lamina epithelium and intestinal glands of the lamina propria were numerous in number at D28 than the chickens at D14 and at D1. DOI: http://dx.doi.org/10.3329/jbau.v10i1.12096 J. Bangladesh Agril. Univ. 10(1): 69–77, 2012

Chinese highly pathogenic porcine reproductive and respiratory syndrome virus exhibits more extensive tissue tropism for pigs

BackgroundThe highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) emerging in China exhibits high fatality to pigs. However, the mechanism related to the increased pathogenicity of the virus remains unclear. In the present study, the differences in tissue tropism between the highly pathogenic PRRSV strain (JXwn06) and the low pathogenic PRRSV strain (HB-1/3.9) were investigated using PRRSV-specific immunohistochemistry (IHC) staining to provide evidence for elucidating possible mechanism of the pathogenicity of Chinese highly pathogenic PRRSV.FindingsIHC examination showed that PRRSV antigen in the tissues including spleen, tonsil, thymus, kidney, cerebellum, stomach, small intestine, large intestine, turbinal bone and laryngeal cartilage was positive in more pigs inoculated with JXwn06 than HB-1/3.9, and the tissues including trachea, esophagus, liver, mandibular gland and thyroid gland were positive for viral antigen in the pigs inoculated with JXwn06, but not in the pigs inoculated with HB-1/3.9. Meanwhile, we observed that epithelium in tissues including interlobular bile duct in liver, distal renal tubule of kidney, esophageal gland and tracheal gland were positive for viral antigen only in JXwn06-inoculated pigs, and epithelium of gastric mucosa and fundic gland, and intestinal gland were positive for viral antigen in both JXwn06- and HB-1/3.9-inoculated pigs, using monoclonal antibodies to N and Nsp2 proteins.ConclusionsTaken together, these findings indicate that the highly pathogenic PRRSV JXwn06 displays an expanded tissue tropism in vivo, suggesting this may contribute to its high pathogenicity to pigs.

A new species of Philometra Costa, 1845 (Nematoda: Philometridae) from the freshwater fish (red piranha) Pygocentrus nattereri Kner (Characidae) in Amazonia, Brazil.

A new nematode species, Philometra nattereri n. sp. (Philometridae), is described from female specimens found in the oculo-orbits and nasal cavity of the red piranha Pygocentrus nattereri Kner (Characiformes: Characidae) from five lakes in Central Amazonia, Brazil, collected in 2008 and 2009 (overall prevalence 12%, intensity 1-3 nematodes per fish). Based on light and scanning electron microscopical examination, the new species differs from most other congeners parasitising freshwater fishes in that its oesophageal gland extends anteriorly far anterior to the level of the nerve-ring, in the presence of 14 small cephalic papillae arranged in two circles and in having two minute caudal projections. This is the first species of Philometra Costa, 1845 reported from fishes of the family Characidae and the second valid species of this genus parasitic in freshwater fishes of Brazil and South America.

SiRNAs Trigger Efficient Silencing of a Parasitism Gene in Plant Parasitic Root-Knot Nematodes

Expanding genomic data on plant pathogens open new perspectives for the development of specific and environment friendly pest management strategies based on the inhibition of parasitism genes that are essential for the success of infection. Identifying such genes relies on accurate reverse genetics tools and the screening of pathogen knock-down phenotypes. Root-knot nematodes are major cosmopolitan crop pests that feed on a wide range of host plants. Small interfering RNAs (siRNAs) would provide a powerful tool for reverse genetics of nematode parasitism genes provided that they could (1) target genes expressed in inner tissues of infective nematodes and (2) target genes expressed during parasitism. In this study, we show that siRNAs can access inner tissues of the infective juveniles during soaking and accumulate in the esophagus, amphidial pouches and related neurons of the nematode. We provide evidence that siRNAs can trigger knock-down of the parasitism gene Mi-CRT, a calreticulin gene expressed in the esophageal glands of Meloidogyne incognita. Mi-CRT knock-down in infective juveniles affected nematode virulence. However, Mi-CRT knock-down was not persistent after plant infection, indicating that siRNA-mediated RNAi is best suited for functional analysis of genes involved in pre-parasitic stages or in the early steps of infection.

Putative Phage Hyperparasite in the Rickettsial Pathogen of Abalone, “Candidatus Xenohaliotis californiensis”

Studies on the ecology of microbial parasites and their hosts are predicated on understanding the assemblage of and relationship among the species present. Changes in organismal morphology and physiology can have profound effects on host-parasite interactions and associated microbial community structure. The marine rickettsial organism, "Candidatus Xenohaliotis californiensis" (WS-RLO), that causes withering syndrome of abalones has had a consistent morphology based on light and electron microscopy. However, a morphological variant of the WS-RLO has recently been observed infecting red abalone from California. We used light and electron microscopy, in situ hybridization and16S rDNA sequence analysis to compare the WS-RLO and the morphologically distinct RLO variant (RLOv). The WS-RLO forms oblong inclusions within the abalone posterior esophagus (PE) and digestive gland (DG) tissues that contain small rod-shaped bacteria; individual bacteria within the light purple inclusions upon hematoxylin and eosin staining cannot be discerned by light microscopy. Like the WS-RLO, the RLOv forms oblong inclusions in the PE and DG but contain large, pleomorphic bacteria that stain dark navy blue with hematoxylin and eosin. Transmission electron microscopy (TEM) examination revealed that the large pleomorphic bacteria within RLOv inclusions were infected with a spherical to icosahedral-shaped putative phage hyperparasite. TEM also revealed the presence of rod-shaped bacteria along the periphery of the RLOv inclusions that were morphologically indistinguishable from the WS-RLO. Binding of the WS-RLO-specific in situ hybridization probe to the RLOv inclusions demonstrated sequence similarity between these RLOs. In addition, sequence analysis revealed 98.9-99.4% similarity between 16S rDNA sequences of the WS-RLO and RLOv. Collectively, these data suggest that both of these RLOs infecting California abalone are "Candidatus Xenohaliotis californiensis," and that the novel variant is infected by a putative phage hyperparasite that induced morphological variation of its RLO host.

Adenoid Cystic Carcinoma of Lacrimal Gland

Adenoid cystic carcinoma is a relatively uncommon tumour of salivary, glands and is characterised by a prolonged clinical course and a fatal outcome. It was first described as `cylindroma' by Billroth in 1859. Half of these tumors occur in glandular tissues other than the major salivary glands; principally in the hard palate, but they can also arise in the tongue and minor salivary glands. Unusual locations include the external auditory canal, nasopharynx, lacrimal glands, breast, vulva, esophagus, cervix and Cowper glands. The long natural history of this tumor and its tendency for local recurrence are well known. JMS 2012;15(1):76-77.

Tissue expression patterns of Schistosoma mansoni Venom Allergen-Like proteins 6 and 7

The Schistosoma mansoni Venom Allergen-Like proteins (SmVALs) are members of the SCP/TAPS (Sperm-Coating Protein/Tpx-1/Ag5/PR-1/Sc7) protein superfamily, which may be important in host–pathogen interactions. Whole mount in situ hybridisation demonstrated a distinct expression pattern in oral and ventral suckers of adult worms for SmVAL6 and in the oesophageal gland for SmVAL7 transcripts, respectively. Additionally, immunocytochemistry analysis corroborated SmVAL7 expression in the oesophageal gland. Analysis of protein expression across the parasite’s life cycle revealed that the SmVAL6 protein is upregulated in cercariae and adult male worms. Furthermore, SmVAL6 protein was identified by mass spectrometry in tegument fractions of adult worms. Finally, we speculate on possible functions of these two SmVALs at the host–parasite interface.

A root‐knot nematode‐secreted protein is injected into giant cells and targeted to the nuclei

Root-knot nematodes (RKNs) are obligate endoparasites that maintain a biotrophic relationship with their hosts over a period of several weeks and induce the differentiation of root cells into specialized feeding cells. Nematode effectors synthesized in the oesophageal glands and injected into the plant tissue through the syringe-like stylet certainly play a central role in these processes. In a search for nematode effectors, we used comparative genomics on expressed sequence tag (EST) datasets to identify Meloidogyne incognita genes encoding proteins potentially secreted upon the early steps of infection. We identified three genes specifically expressed in the oesophageal glands of parasitic juveniles that encode predicted secreted proteins. One of these genes, Mi-EFF1 is a pioneer gene that has no similarity in databases and a predicted nuclear localization signal. We demonstrate that RKNs secrete Mi-EFF1 within the feeding site and show Mi-EFF1 targeting to the nuclei of the feeding cells. RKNs were previously shown to secrete proteins in the apoplasm of infected tissues. Our results show that nematodes sedentarily established at the feeding site also deliver proteins within plant cells through their stylet. The protein Mi-EFF1 injected within the feeding cells is targeted at the nuclei where it may manipulate nuclear functions of the host cell.

Abstract 3424: Cancer cells lose their expression of lipoxygenases in order to activate pro-survival and angiogenic pathways thereby establishing successful macro-metastases

Abstract Metastasis is the major cause of cancer mortality. We therefore wanted to study the possible genetic changes acquired by these cancer cells in order to be endowed with metastatic capabilities. Several studies have identified genes which are differentially expressed in primary and secondary tumor growths. 15-lipoxygenase (ALOX15B) is one such gene. It encodes for an enzyme that takes part in arachidonic acid metabolism and has been characterized as a functional tumor suppressor in prostate cancer. The expression and activity of ALOX15B is frequently suppressed during the carcinogenic progress in the prostate, lungs, esophagus and sebaceous glands. However the exact role of ALOX15B as a tumor suppressor or the underlying mechanism behind its suppression in metastatic cancer cells remains largely unknown. Our initial in silico analyses from clinical samples showed that ALOX15B expression is attenuated in metastatic prostate cancer, compared to normal tissue or primary prostate cancer. Gene array studies across several cancer cell lines showed that ALOX15B expression is inversely correlated with invasiveness of the cell type. Gene clustering showed that ALOX15B is grouped with genes associated with an epithelial phenotype. ALOX15B expression was knocked down in immortalized non-transformed prostate as well as non/low invasive prostate cancer cell line. This had no effect on the proliferative indices in these cells, neither were the cells more resistant to apoptosis under normal growth conditions. However the cells were endowed with increased resistance to hypoxic stress. This was at least in part achieved by increased production of VEGF-A. Analyses of several pro-survival pathways identified that Akt signaling was hyper activated in these ALOX15B knocked down cells. This in turn led to a decreased FoxO transcriptional activity. Conversely, restoration of ALOX15B in low/non metastatic as well as highly metastatic prostate cancer cell lines decreased their basal level of Akt signaling along with increased FoxO activity and a concomitant decrease in VEGF-A production. Further experiments showed that ALOX15B regulates Akt activity by modulating its phosphorylation status. Luciferase tagged rat prostate cancer cells were engineered to over-express ALOX15B. Implantation of such cells in mice showed that cells with restored expression of ALOX15B showed no difference in growth kinetics of primary tumor growth, but a decrease in luciferase activity in the liver of such mice. To summarize, our data shows that loss of ALOX15B expression in prostate cancer cells endows such cells with pro-survival benefits which allows them to establish successful macro-metastases. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3424. doi:1538-7445.AM2012-3424

The effect of ethanol on the formation of N 2-ethylidene-dG adducts in mice: implications for alcohol-related carcinogenicity of the oral cavity and esophagus

The present study aimed to experimentally confirm that long-term alcohol drinking causes a high risk of oral and esophageal cancer in aldehyde dehydrogenase 2 (ALDH2)-deficient individuals. Aldh2 knockout mice, an animal model of ALDH2-deficiency, were treated with 8% ethanol for 14 months. Levels of acetaldehyde-derived DNA adducts were increased in esophagus, tongue and submandibular gland. Our finding that a lack of Aldh2 leads to more DNA damage after chronic ethanol treatment in mice supports epidemiological findings on the carcinogenicity of alcohol in ALDH2-deficient individuals who drink chronically.

Ectopic Sebacious Glands in the Esophageal Mucosa

A white woman was referred to our department for further evaluation of pain in the right upper abdomen. er past medical history was significant for gastroesophageal eflux disease and rheumatoid arthritis. Physical examination as unremarkable. Gallbladder ultrasound revealed a 1-cm emangioma of the liver, otherwise unremarkable. An upper astrointestinal endoscopy showed patchy mild mucosal abnorality characterized by yellow circular spots in the middle third f the esophagus (30 cm from the upper incisors). Biopsies were aken from these areas (Figure A, B). The rest of the esophagus, tomach, and duodenum revealed no abnormality except for iny gastric erosions. Pathology of esophageal biopsy showed fragments of esophaeal mucosa showing sebaceous gland formation. The surroundng epithelium was histologically unremarkable (Figure C). Sebaceous glands in the esophagus are rare.1-5 The association between gastroesophageal reflux disease and presence of esophageal sebaceous glands is unknown. Sebaceous glands are normally found in association with hair follicles.1 Ectopic sebaceous glands have been reported in other tissues of ectodermal origin like the palms and soles, nipple, genitals, eyes and orbits, and parotid glands.3,4 The histogenesis of sebaceous glands in the esophagus is not clear. Several hypotheses have been postulated.3 The embryoogic misplacement of sebaceous glands at ectodermal tissue and metaplasia of submucosal glands are the 2 important hypotheses. The observation that most of the reported sebaceous glands were found in older patients supports the theory of metaplasia.3

Cervical inlet patch-optical coherence tomography imaging and clinical significance

To demonstrate the feasibility of optical coherence tomography (OCT) imaging in differentiating cervical inlet patch (CIP) from normal esophagus, Barrett's esophagus (BE), normal stomach and duodenum. This study was conducted at the Veterans Affairs Boston Healthcare System (VABHS). Patients undergoing standard esophagogastroduodenoscopy at VABHS, including one patient with CIP, one representative patient with BE and three representative normal subjects were included. White light video endoscopy was performed and endoscopic 3D-OCT images were obtained in each patient using a prototype OCT system. The OCT imaging probe passes through the working channel of the endoscope to enable simultaneous video endoscopy and 3D-OCT examination of the human gastrointestinal (GI) tract. Standard hematoxylin and eosin (H and E) histology was performed on biopsy or endoscopic mucosal resection specimens in order to compare and validate the 3D-OCT data. CIP was observed from a 68-year old male with gastroesophageal reflux disease. The CIP region appeared as a pink circular lesion in the upper esophagus under white light endoscopy. OCT imaging over the CIP region showed columnar epithelium structure, which clearly contrasted the squamous epithelium structure from adjacent normal esophagus. 3D-OCT images obtained from other representative patients demonstrated distinctive patterns of the normal esophagus, BE, normal stomach, and normal duodenum bulb. Microstructures, such as squamous epithelium, lamina propria, muscularis mucosa, muscularis propria, esophageal glands, Barrett's glands, gastric mucosa, gastric glands, and intestinal mucosal villi were clearly observed with OCT and matched with H and E histology. These results demonstrated the feasibility of using OCT to evaluate GI tissue morphology in situ and in real-time. We demonstrate in situ evaluation of CIP microstructures using 3D-OCT, which may be a useful tool for future diagnosis and follow-up of patients with CIP.

Identification of Distinct Gene Expression Profiles between Esophageal Squamous Cell Carcinoma and Adjacent Normal Epithelial Tissues

Esophageal squamous cell carcinoma (ESCC) is a predominant type of esophageal cancer, which is a malignant tumor originating from the esophageal mucosa or gland and is aggressive with poor prognosis. Identification of new gene expression patterns would be helpful for providing new targets for the early detection and treatment of ESCC patients. In the present study, we employed cDNA array technology to compare gene expression profiles between ESCC tissues and adjacent normal epithelial tissues from ESCC patients. There was at least a 4-fold change in the expression levels of 72 genes that were significantly increased and 107 genes that were decreased in ESCC compared with normal esophageal epithelium. Among them, genes known to be involved in ESCC were found, including matrix metalloproteinases, transcription factors SOX-4 and SOX-17, the Wingless-type MMTV integration site family member 2, and cell cycle regulators. Moreover, we have newly identified the two genes that are down-regulated in ESCC: monoamine oxidase A, an enzyme that catalyzes monoamines oxidation and 15-hydroxyprostaglandin dehydrogenase [NAD+], a prostaglandin-synthesizing enzyme that physiologically antagonizes COX-2. Likewise, we found the three genes that are up-regulated in ESCC: CD7, a cell surface glycoprotein member of the immunoglobulin superfamily, LIM-domain kinase 1, a small subfamily with an unique combination of two N-terminal LIM motifs and a C-terminal protein kinase domain, and TTK protein kinase, a previously unidentified member of the kinase family. These newly identified genes may be involved in the progression of the tumor and/or represent properties specific to ESCC.

A Case of Esophageal Submucosal Gland Duct Adenoma

식도 ì ë§‰í•˜ 도관 ì„ ì¢ ì€ 드문 질환으로 대부분의 ì‹ë„ì„ ì¢ ê³¼ëŠ” 달리 ë°”ë ›ì‹ë„ 없이 식도 ê³ ìœ ì„ ì—ì„œ 발생한다. 위 내시경을 통한 ìœ¡ì•ˆì  검사로는 감별진단이 ì–´ë ¤ìš°ë©° 내시경 초음파에서 ë‚­ì¢ ì™¸ë²½ì´ 두껍거나 ë‚­ì¢ ë‚´ 격막이 관찰되면 의심해 ë³¼ 수 있다. íŠ¹ì§•ì ì¸ 조직소견은 ë‚­ì¢ ë‚´ë¶€ 도관 상피세포와 외부 ê·¼ 상피세포로 이루어진 두층 구조를 가진 조직이 증식한다는 ì ì´ë‹¤. 본 증례는 건강검진에서 우연히 발견된 식도 ì ë§‰í•˜ 병변을 ë‚´ì‹œê²½ì  ì ˆì œìˆ ì„ 시행하여 도관 ì„ ì¢ ì„ ì§„ë‹¨í•˜ì˜€ê³ êµ­ë‚´ì— ë³´ê³ ëœ 바 없어 ë¬¸í—Œê³ ì°°ê³¼ 함께 ë³´ê³ í•˜ëŠ” 바이다. 중심 단어: 식도 도관 ì„ ì¢ ; ì ë§‰í•˜ ì¢ ì–‘; ë‚´ì‹œê²½ì  ì ˆì œìˆ