Oenological Tannins Research Articles

Review on oenological tannins: conventional and emergent extraction techniques, and characterization

Review on oenological tannins: conventional and emergent extraction techniques, and characterization

The effect of probe density coverage on the detection of oenological tannins in Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) experiments.

Polyphenols are a group of compounds found in grapes, musts, and wines. Their levels are crucial for grape ripening, proper must fermentation, and final wine characteristics. Standard chemical analysis is commonly used to detect these compounds, but it is costly, time-consuming, and requires specialized laboratories and operators. To address this, this study explores a functionalized acoustic sensor for detecting oenological polyphenols. The method involves utilizing a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect the target analyte by using a gelatin-based probe layer. The sensor is functionalized by optimizing the probe coverage density to maximize its performance. This is achieved by using 12-mercaptododecanoic acid (12-MCA) to immobilize the probe onto the gold sensor surface, and dithiothreitol (DTT) as a reducing and competitive binding agent. The concentration of 12-MCA and DTT in the solutions is varied to control the probe density. QCM-D measurements demonstrates that the probe density can be effectively adjusted using this approach, ranging from 0.2×1013 molecules cm-2 to 2×1013 molecules cm-2 . This study also investigates the interaction between the probe and tannins, confirming the sensor ability to detect them. Interestingly, the lower probe coverage achieves higher detection signals when normalized to probe immobilization signals. Moreover, significant changes in mechanical properties of the functionalization layer are observed after the interaction with samples. The combination of QCM-D with gelatin functionalization holds great promise for future applications in the wine industry. It offers real-time monitoring capabilities, requires minimal sample preparation, and provides high sensitivity for quality control purposes. This article is protected by copyright. All rights reserved.

Characterization of 20 Oenological Tannins from Different Botanical Origins for Formulation of Blends with Redox Potential Tuning Ability in Model Wine Solution.

Twenty oenotannins from different botanical origins were studied in model wine solution (1 g/L, 12% ethanol, pH 3.5). An original device was created for measuring Oxidation-Reduction potential (ORp) of the solutions at 20 °C in strict anoxic condition by the electrochemical method of the platinum electrode zero-current potential. Reactivity against proteins and antioxidant properties were related to the chemical structure and, consequently, to the botanical origin of the oenotannins. The highest turbidity after BSA addition (ΔNTU > 1000) values were measured for the gallic hydrolysable tannins. The ORp versus standard hydrogen electrode ranged from 420 to 260 mV. The ellagitannins had the highest antioxidant power (AP%), followed by condensed tannins and gallotannins, highlighting a correlation with the phenolic profile. Based on these findings, two formulations were prepared as a blend of some of the tested oenotannins, with the ability to increase (MIX1) and decrease (MIX2) the ORp of the model wine.

Insight into the characterization of commercial oenological tannins

The characterization in terms of phenolics, antioxidant capacity and oxygen consumption rate (OCR) was carried out for 15 commercial oenological tannins of different origin. A NMR approach was used to evaluate their average molecular weight, and their glycosidic and aromatic moieties. The investigated oenological tannins showed wide differences in their chemical properties. Total phenol index (TPI) ranged from 461±28 and 1018±57 mg gallic acid/g for cherry tannin and nut gall tannin, respectively. The antioxidant capacity, positively correlated with TPI, was higher for hydrolysable tannins ranging 3.05±0.06 and 12.06±0.71 mM Trolox/g for ABTS assay, and from 3.70±0.23 and 10.94±1.28 mM Trolox/g for DPPH assay. Relevant differences in OCRs were found and chestnut tannin showed the highest OCR. Wide range of molecular weights were found with nut gall 1 tannin showing the highest one, ranging from 790 to 1900 Da. This study improves and expands the actual knowledge of tannins supporting the suitability of NMR for the characterization of oenological tannins.

Use of Oenological Tannins to Protect the Colour of Rosé Wine in a Bioprotection Strategy with Metschnikowia pulcherrima.

Although bioprotection is now recognised as an alternative to SO2 for limiting microbial spoilage, it does not guarantee protection against oxidation. This limits its application, more specifically for rosé winemaking. Oenological tannins present antioxidant properties, which could represent an interesting alternative to SO2 to protect must and wines against oxidation. A combination of the inoculation of a bioprotectant yeast strain and the addition of oenological tannins was tested to eliminate sulfites during the pre-fermentative step of rosé winemaking. In this experiment carried out in a winery, two oenological tannins were compared: quebracho and gall nut tannins. The antioxidant efficiency of tannins was compared to that of SO2. Colorimetric assays associated with chemical analyses of anthocyanins and phenolic compounds confirmed that the use of bioprotection alone did not protect the wine from oxidation. An addition of oenological tannins on musts stabilized the colour of bioprotected rosé wine in a similar way that SO2 addition did. Quebracho tannins appeared more efficient than gall nut tannins. The colour differences observed cannot be explained either by the concentration or forms of anthocyanins. However, the addition of tannins led to better protection of oxidation-sensitive phenolic compounds comparable to that obtained with the addition of sulfites.

Cover Image

The cover image is based on the Research Article Developing novel oenological tannins from 44 plants sources by assessing astringency and color in model wine by Chenyaqiong Cheng et al., https://doi.org/10.1002/jsfa.12247.image

Research on the reduction of SO2 doses in winemaking using lysozyme and oenological tannin

Research on the reduction of SO2 doses in winemaking using lysozyme and oenological tannin

Developing novel oenological tannins from 44 plants sources by assessing astringency and color in model wine.

Oenological tannins are commercial natural products extracted from different botanical sources, which were widely reported as prominent contributors to wine quality. Research on wine quality affected by tannins extracts promoted the development of new oenological products with low cost and high accessibility. In the present study, the structure and concentration of tannin in polyphenol extracts, as well as their correlation with astringency and the color of model wine, was investigated by UV spectrophotometer, HPLC, fluorescence quenching, sodium dodecylsulfate-polyacrylamide gel electrophoresis, colorimeter and sensory evaluation. Resource extracts from 16 of 44 plants were screened as wine oenological tannins, according to the total polyphenol and total flavanol, as well as the intensity of astringency and bitterness. Polyphenols extracted from grape seeds and green tea were more effective in increasing the wine astringency compared to other plant tannins. Total flavanol content and tannin activity showed a strong correlation with wine astringency. Condensed tannins with mean degree of polymerization also exhibited strong color stability, and the concentrations of (-)-epigallocatechin were associated with the a* value, a negative qualitative factor for wine color. The present study provides new clues regarding the development of low-cost and highly accessible sources of polyphenol extracts and lays a theoretical foundation for the development of the oenological product. © 2022 Society of Chemical Industry.

Evaluation of oenological tannins for preventing the light-struck taste

The light-struck taste (LST) is a fault occurring in white and rosé wines associated to the formation of volatile sulfur compounds (VSCs) due to the reactions between riboflavin (RF) and methionine (Met). We investigated the possible preventing effect of 15 commercial tannins of different origin in model wine added with RF and Met, under oxic and anoxic conditions, and submitted to standardized light-exposure. All the tannins limited the degradation of Met in comparison to the tannin-free samples. Lower concentrations of VSCs were found in presence of tannins even under anoxia, condition favouring their formation. The sniffing trials evidenced the minor perception of cooked cabbage note with added tannins. The multivariate analysis showed the presence of flavan-3-ols was related to the formation of DMDS and DMTS, while tannic acid was related to MeSH, both the behaviors occurring under oxic condition. The study highlighted the ability of tannins to prevent the LST.

Relative impact of oenological tannins in model solutions and red wine according to phenolic, antioxidant, and sensory traits

Oenological tannins (OETs) are winemaking processing aids used to facilitate stabilization and fining, to increase the antioxidant capacity, and to promote colour stability of grape juice and wine. A wide variability of pure or mix formulates are available for winemaking purposes, including hydrolysable tannins (gallotannins and ellagitannins), proanthocyanidins from grape skins and seeds (prodelphinidins and procyanidins), and from exotic wood (prorobinetinidins and profisetinidins). In this study, seventeen OETs pure and mix formulates were characterized in terms of polyphenolic content and antioxidant capacity in a model wine and in a red wine after one-month storage, as well as aroma, astringency, and bitterness sensory characteristics in water and red wine. Colour-related features were also analysed in the added red wine after one-month storage. For the first time, correlations among the obtained results in the different matrices were investigated to understand the most suitable OETs for winemaking applications. The results showed a great variability among the formulates studied in terms of phenolic content, which was strictly correlated to their antioxidant capacity. Regarding origin, hydrolysable tannins had the highest antioxidant ability, followed by exotic wood formulates. A strong and positive correlation was found in antioxidant capacity of OETs in model wine and red wine after one-month storage, in particular for ellagitannins, which confirmed also their ability to increase pigments polymerization. By contrast, quebracho tannins resulted the bitterest and most astringent when tasted in water (0.4 g/L), although in-mouth and aromatic descriptors of OETs tasted in water were not correlated with the ones of the added red wine. Therefore, the choice of OETs formulate and its optimal dose requires a characterization in terms of polyphenolic content and antioxidant capacity because these properties were well correlated with those of the added wines in a short storage period, whereas the sensory impact at oenological range doses is mainly dependent on wine features.

Influence of tannins and mannoproteins products on the improvement of organoleptic characteristics of wines

The aim of the work was to establish an influence of oenological tannins and mannoproteins products on the change of the organoleptic parameters of table dry wines, made from grapes of aromatic and non-aromatic varieties. The materials of the researches were wines that had defects or faults, mainly related with changes in wine aroma and taste. Oenological tannins of various botanical origins and mannoproteins products of a company Martin Vialatte (France) were used in the researches. Wines with these additives were mixed intensively, kept during 3 days at a temperature, not exceeding 12 °C and then determined the sensory characteristics of wine. Control samples were wines without additives. The descriptive method of analysis was used to create aromatic profiles of wines. The effectiveness of tannins and mannoproteins products on the sensory characteristics of wines was established. The intensity of the descriptors that made a positive affect on the aroma and taste of the samples was increasing. If, at the beginning tones of oxidation or bitterness were felt in the wines, then after introduction of the additives, these tones decreased in intensity. A difference of the changes of aromatic characteristics of wines from two groups of grapes with different aromatics was established. The action of additives was spread on the tings of descriptors, enriching the aroma of samples from non-aromatic grape varieties together with eliminating faults of taste. The effect of the additives was light in the aromatic varieties of grape that is due to the strong aroma and expressed taste of the grapes of these varieties. The results of the researches can be used to improve the quality of white wines, and further researches should be focused on the expansion of assortments of wines from other varieties, as a result utilizing tannins and mannoprotein products

THE CURRENT STATE AND TRENDS OF PROCESSING SECONDARY RAW MATERIALS OF WINEMAKING IN UKRAINE

The current state of processing secondary raw materials of winemaking in Ukraine has been analysed. It has been shown that these materials are a rich source of bioactive compounds. This allows using them to manufacture a wide range of products (oenological tannin, food oenocolourant, polyphenolic extracts, tartaric acid, beverages, grape oil, vitamin D, protein, animal feed, food powder, fertilisers, abrasive materials, etc.) with high consumer value for various industries: food, pharmaceutical, perfume and cosmetics, chemical, compound feed, etc. In the light of modern views, phenolic compounds contained in large quantities in grape stems and pomace have been shown to be indispensable factors in nutrition and treatment. It has been noted that in today’s Ukraine, there are no specialised enterprises for complex processing of secondary raw materials of winemaking. In particular, unprocessed grape stalks and pomace are in most cases uncontrollably carried away to farmlands. This leads to acid erosion of the soil and to polluting the environment by micromycete metabolites, which but exacerbates one of mankind’s global problems, the environmental one. It has been concluded that traditional domestic technologies of processing secondary raw materials of winemaking are technologically, economically, and environmentally ineffective. There is no comparative analysis of innovative domestic and foreign technologies and equipment for processing secondary raw materials of winemaking. Modern innovations to obtain bioactive additives and other products cannot be introduced, since there is no necessary home-manufactured equipment, and imported machines are too expensive. Besides, there is but weak interaction among wineries, research institutions, business structures, and administrative authority. Cluster ideology has been suggested as a basis to organise comprehensive processing of secondary raw materials of winemaking in Ukraine. This will unite the interests of wineries (producers of secondary raw materials), processing enterprises (manufacturers of products from secondary raw materials), research institutions, and potential consumers of innovative products

Effects of oenological tannins on aroma release and perception of oxidized and non-oxidized red wine: A dynamic real-time in-vivo study coupling sensory evaluation and analytical chemistry

Addition of oenological tannins claims to have a positive impact on wine stability, protection from oxidation and likely sensory persistence. However, their role on red wine aroma during oxidation is controversial. The present study aims at investigating the effect of addition of oenological tannins on wine flavour (mainly aroma) before and after air exposure. Temporal Dominance of Sensations, a dynamic sensory evaluation, was coupled with a dynamic chemical measurement (nosespace analysis) using a Proton-Transfer-Reaction Mass-Spectrometer connected to the nasal cavity of 17 assessors. Results showed that the oxidation of a non-oaked Pinot Noir red wine decreases the fruity aroma dominance and increases the maderised and prune one. A contextual decrease of the fruity ethyl decanoate and increase of oxidative Strecker aldehydes are observed. Ellagitannins but not proanthocyanidins preserved perception of fruitiness and prevented increase of maderised notes. Moreover, ellagitannins increase the aroma persistence mainly in the non-oxidized wine.

Impacts of added oenological tannins on red wine quality to counteract <i>Botrytis</i> infection in Merlot grapes

The contamination of grape berries by Botrytis cinerea can drastically damage wine quality, in particular causing colour degradation. In musts obtained from botrytised grapes, SO2 addition is the main means of avoiding oxidation damage due to laccases excreted by the pathogen. However, consumers are becoming increasingly reluctant to accept SO2 addition to wine. Oenological tannins are used for fining wines because of their colloidal properties, and for wine colour stabilisation due to their ability to condense with anthocyanins. They are also known for their antioxidant and antioxidasic properties. They were thus investigated in the present study for their potential as an alternative to SO2 against laccase oxidation.The impact of various types of oenological tannins on musts and wines was studied once added to musts obtained from the Merlot cultivar, comprising 20 % and 50 % botrytised grapes. Laccase activity, antioxidant capacity, composition of phenolic compounds, spectrophotometric and CIELAB colour parameters were assessed in the musts and wines. Sensory analyses were also performed on 3-month-old wines to evaluate the visual, olfactory and gustative consequences of tannin addition. At a 50 % botrytisation rate, the addition of any type of oenological tannins (at a concentration of 100 g/hL) had no effect on laccase activity and did not protect phenolic compounds. However, at the same concentration and at a 20 % botrytisation rate, proanthocyanidin tannins from grape skin were found to be the most promising tannins with simultaneous protective effects, such as an inhibitory effect on laccase enzymes, protection of colour from complete degradation and preservation of some procyanidin compounds.Oenological tannins are promising candidates for protecting wines from Botrytis damage and they induce a differential effect according to their origin and structure. They could be used to reduce the amount of SO2 that is added during vatting. More research is needed to confirm and better understand the mode of action of various tannins at levels lower than the 20 % botrytised rate tested in the present study.

Can a Corn-Derived Biosurfactant Improve Colour Traits of Wine? First Insight on Its Application during Winegrape Skin Maceration versus Oenological Tannins.

In winemaking, oenological tannins are used to preserve wine colour by enhancing the antioxidant activity, taking part in copigmentation, and forming polymeric pigments with anthocyanins. As a novel processing aid, in this study, a biosurfactant extract was evaluated as a solubilizing and stabilizing agent of anthocyanins in red wine. The biosurfactant extract under evaluation was obtained from a fermented residual stream of the corn milling industry named corn steep liquor (CSL). Two red winegrape varieties (Vitis vinifera L. cv. Aglianico and Cabernet sauvignon) were studied for anthocyanin content and profile, and colour traits, during simulated skin maceration for 7 days at 25 °C, as well as polymerization and copigmentation at the end of maceration. A model wine solution was used as a control, which was added either with the CSL biosurfactant or with four different oenological tannins (from grape skin, grape seed, quebracho, and acacia). The results showed that CSL biosurfactant addition improved the colour properties of skin extracts by the formation of more stable compounds mainly through copigmentation interactions. These preliminary results highlighted that the effectiveness of CSL biosurfactant is variety-dependent; however, there is no significant protection of individual anthocyanin compounds as observed for delphinidin and petunidin forms using quebracho tannin.

How to Deal with Uninvited Guests in Wine: Copper and Copper-containing Oxidases

Copper is one of the most frequently occurring heavy metals in must and wine. It is introduced by pesticides, brass fittings, and as copper sulphate for treatment of reductive off-flavors. At higher concentrations, copper has harmful effects on the wine. It contributes to the oxidation of wine ingredients, browning reactions, cloudiness, inhibition of microorganisms, and wine fermentation. Last but not least, there is also a danger to the consumer. At present, some physicochemical methods exist to reduce the copper content in must and wine, but they all have their shortcomings. A possible solution is the biosorption of metals by yeasts or lactobacilli. Copper can also reach must and wine in the form of copper-containing phenol oxidases (grape tyrosinase, Botrytis cinerea laccases). Similar to free copper, they oxidize phenolic wine compounds, and thus lead to considerable changes in color and nutritional value, making the product ultimately unsaleable. All measurements for enzyme inactivation such as heat treatment, and addition of sulphites or bentonite are either problematic or not effective enough. The application of oenological tannins could offer a way out but needs further research.

Role of anthocyanin traits on the impact of oenological tannins addition in the first stage of red winegrape skin simulated maceration

In winemaking, exogenous tannins are added before maceration to improve future wine color characteristics derived from extracted grape anthocyanins. The study aimed to investigate the relation between different grape varieties, selected according to their anthocyanin profile, and the effect of five exogenous tannin formulations differing in origin and chemical features. Anthocyanin content, polymeric pigments, and color traits were assessed during a 72-hour skin simulated maceration. Grape skin-derived tannins increased color intensity (up to one unit) and polymeric pigments formation (up to 6.5%) in malvidin-prevalent Merlot and Cabernet sauvignon, with different extent depending on the anthocyanin richness. Grape seed-derived and ellagic formulations favored the pigment polymerization, the first in Nebbiolo and Sangiovese (up to 8.2%), which are characterized by high ratios of disubstituted anthocyanins, and the latter in malvidin-rich Syrah and Aglianico (up to 5%). A positive effect of quebracho regarded the defense of anthocyanin forms, particularly in Sangiovese and Nebbiolo.

The Oxygen Consumption Kinetics of Commercial Oenological Tannins in Model Wine Solution and Chianti Red Wine.

One property of oenological tannins, oxygen reactivity, is commonly exploited in winemaking. The reactivity is mediated by the presence of catalysts (i.e., transition metals and sulfur dioxide) and protects wine against oxidation. This work compares the oxygen consumption rate (OCR) of four commercial oenological tannins (two procyanidins from grape skin and seed, an ellagitannin from oak wood and a gallotannin from gallnut) in a model wine solution and Chianti red wine. All samples were subjected to consecutive cycles of air saturation at 20 °C to increase the total level of oxygen provided. After each cycle, the oxygen level was measured by means of a non-invasive luminescent sensor glued to a transparent surface (sensor dots) until there was no further change in substrate reactivity. The OCR followed first-order kinetics, regardless of the tannin. As expected, the ellagitannin showed the fastest OCR, followed by the two from grape seeds and skins and finally the gallotannin. The total O2 consumption in the red wine was almost double that of the model solution, due to the oxidation of wine substrates. The measurement of OCR is helpful for setting up an advanced winemaking protocol that makes use of tannins to reduce the use of sulfur dioxide.

Oenological tannins to prevent Botrytis cinerea damage in grapes and musts: Kinetics and electrophoresis characterization of laccase

Enzymatic parameters (KM and Vmax), residual activity, effect of bentonite and electrophoresis characterization of laccase in the presence of different oenological tannins (OT) were investigated in relation to B. cinerea negative effects in grapes and musts. Five OT were tested (gallotannin, ellagitannin, quebracho, grape-skin and grape-seed) in comparison with ascorbic acid (AA), sulfur dioxide (SO2) and bentonite. We added OT, AA, SO2 and bentonite to botrytized must obtained by inoculation of grapes with B. cinerea strain 213. Laccase activity was measured by the syringaldazine method at different concentrations of substrate. Enzymatic parameters were determined using Michaelis-Menten and Lineweaver-Burk plots. The B. cinerea strain was also grown in a liquid medium for laccase production. Molecular weight of laccases and effect of OT upon these laccases were studied by SDS-PAGE. Results confirm that bentonite, contrary to OT, did not permit to reduce laccase activity. Regardless the tannin considered, Vmax, KM and laccase activity were reduced and gallotannin, grape-skin and grape-seed tannin presented the greatest ability. Efficiency of grape-seed tannin addition in order to reduce the laccase activity, was comparable to that of AA or SO2 at the typical doses employed in oenology for each one. Oenological tannins appear to be excellent processing aids to prevent laccase effects and contribute to reduce the use of SO2 in grapes and musts.

Suitability of the Cyclic Voltammetry Measurements and DPPH• Spectrophotometric Assay to Determine the Antioxidant Capacity of Food-Grade Oenological Tannins

Twenty commercially available oenological tannins (including hydrolysable and condensed) were assessed for their antiradical/reducing activity, comparing two analytical approaches: The 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging spectrophotometric assay and the cyclic voltammetry (CV) electrochemical method. Electrochemical measurements were performed over a −200 mV–500 mV scan range, and integrated anodic currents to 500 mV were used to build a calibration graph with (+)-catechin as a reference standard (linear range: From 0.0078 to 1 mM, R2 = 0.9887). The CV results were compared with the DPPH• assay (expressed as % of radical scavenged in time), showing high correlation due to the similarity of the chemical mechanisms underlying both methods involving polyphenolic compounds as reductants. Improved correlation was observed by increasing the incubation time with DPPH• to 24 h (R2 = 0.925), demonstrating that the spectrophotometric method requires a long-term incubation to complete the scavenging reaction when high-molecular weight tannins are involved; this constraint has been overcome by using instant CV measurements. We concluded that the CV represents a valid alternative to the DPPH• colorimetric assay, taking advantage of fast analysis and control on the experimental conditions and, because of these properties, it can assist the quality control along the supply chain.