Odorata Leaf Research Articles

Biogenic Synthesis of Silver Nanoparticles using Chromolaena odorata Leaf Extract and its Antioxidant, Antimicrobial, and Anticancer Activities

Biogenic Synthesis of Silver Nanoparticles using Chromolaena odorata Leaf Extract and its Antioxidant, Antimicrobial, and Anticancer Activities

Green synthesis of nitrogen-doped TiO2 nanoparticles with exposed {001} facets using Chromolaena odorata leaf extract for photodegradation of pollutants under visible light

Facet-tailored TiO2 nanoparticles (NPs) exhibit exceptional properties due to high surface energy. Conventional strategies for the fabrication of such TiO2 NPs involve harmful chemicals, which necessitates the development of environmentally benign pathways. Plant extract-assisted synthesis has emerged as a promising green alternative to conventional nanomaterial synthesis. This work introduces an innovative method for the synthesis of nitrogen-doped TiO2 (N-TiO2) NPs with exposed {001} facets using the leaf extract of a weed plant Chromolaena odorata, which is commonly known as Siam weed. The synthesis was carried out by sol-gel process with triethylamine (TEA), hydrazine hydrate and urea being the nitrogen precursors. The synthesised N-TiO2 NPs exhibited exposed {001} facets and showed a reduction in band gap. Photo-induced degradation of methylene blue dye was used to analyse the photocatalytic capability of N-TiO2 NPs in the visible range. The effect of N precursor, N dosage and light exposure time on the catalytic efficacy was studied. N-TiO2 NPs derived from TEA with 1 mol.% dopant achieved 98 % degradation in 180 minutes, while those synthesized with hydrazine and urea attained 96 % and 93 %, respectively when compared to 90 % degradation for undoped samples. The N-doping leads to significant advancement of photocatalytic effectiveness of the TiO2 NPs by introducing mid-gap levels in the forbidden energy gap that diminishes the charge carrier-recombination and boost the charge-carrier mobility of TiO2. This along with the existence of high energy facets causes a substantial advancement in the photocatalytic function in the visible region. The proposed method is a sustainable way for synthesising N-TiO2 NPs with exposed {001} facets for environment remediation applications.

Formulation and Antibacterial Essay of Topical Cream Enriched with (Chromolaena odorata) Leaf Extract Originating from Ie Seuem Geothermal Area, District of Aceh Besar, Indonesia

Anti-bacterial creams containing herbs and natural products are important because they provide a safe and effective alternative to chemical-based products. C. odorata leaf extract contains numerous active chemicals that can be used to formulate antibacterial cream for topical application. This study aims to formulate an antibacterial topical cream containing active chemicals extracted from C.odorata leaf originating from geothermal area, and evaluate physicochemical properties of the formulated topical cream followed by in-vitro antibacterial activity of the formula. The C.odorata leaf extraction was performed by cold extraction using ethanol and its chemical contents was investigated using GC-MS followed by predicted bioactivity profile using a computer software. Physicochemical evaluation performed include organoleptic evaluation, spread ability, pH values, viscosity measurement, and homogeneity test. GC-MS spectra indicates that the ethanolic extract of C.odorata leaf contains 22 compounds dominated by alcohol derivative compounds and terpenoids where most compounds shows bioactivities when simulated using a computer program. The formulated cream corresponds to the standard of topical cream with good physical stability, homogeneity, and viscosity. The pH of the cream was found to be stable over 28 days after formulation. The antibacterial test against S. aureus indicated that the cream is active to inhibit the bacteria where F3 shows better results compared to other formulations and the positive control. The C.odorata leaf extract formulated as topical cream have shown great activity against S.aureus at the concentration of 10% due to various chemical contents including alcohol derivatives and terpenoids.

Microencapsulation of <i>Chromolaena odorata</i> Leaf Extract with Cellulose Esters for the Application as an Eco-Friendly Antibacterial Agent

The aim of this work is to develop an eco-friendly antibacterial agent in the form of microcapsules containing extract from Chromolaena odorata leaves using the solvent evaporation method. The wall materials for encapsulating were tested with various cellulose esters including cellulose acetate (CA), cellulose acetate butyrate (CAB), and cellulose acetate propionate (CAP). The evaluation of microcapsules containing C. odorata leaf extract was focused on their encapsulation efficiency, size, shape, thermal stability, and antimicrobial activities. The results showed that CAB was a suitable wall material for the encapsulation of C. odorata leaf extract. The CAB microcapsules exhibited the highest encapsulation efficiency, which was 65.82 ± 3.07. The size of CAB microcapsules was the smallest, at 1013.3 ± 66.5 nm. According to the thermogravimetric analysis, the prepared microcapsules were able to protect the extract of C. odorata leaves from the environment. Moreover, the CAB microcapsules containing C. odorata leaf extract showed the best antibacterial activities against Escherichia coli ATCC 25922 and Stapphylococcus aureus ATCC 25923. The minimum bactericidal concentration of the microcapsules was 25.6 mg/mL in both bacteria. This study proved the potential application of C. odorata leaf extract as a biomaterial in diverse industries in the future.

INFLUENCE OF AQUEOUS CHROMOLAENA ODORATA (SIAM WEED) LEAF EXTRACT ON THE PERFORMANCE, IMMUNOLOGICAL AND HISTOLOGICAL INDICES OF BROILER CHICKEN

A 42-day trial was conducted to evaluate the effects of Aqueous Chromolaena odorata (Siam weed) leaf extract (ACOLE) on the growth performance, immunology and histological indices of broiler chickens. A total of 200 day old broiler chicks were randomly allotted into four (4) treatment groups consisting of five (5) replicates of 10 birds per replicate in a completely randomized design. Aqueous COLE was administered in their water served on daily basis at 0, 5, 10 and 15mL/L to represent four treatment groups; T1, T2, T3 and T4 respectively. At the end of the study, data were collected on growth performance, blood samples were collected at day 0, 12 and 42 to conduct immunological assessment of the birds and samples of the intestine and liver were collected for histological evaluation. All data collected were subjected to one-way analysis of variance. The Results obtained showed that there were no significant (P>0.05) differences on all growth parameters evaluated except in feed Conversion Ratio (FCR). Birds in T2 had the best FCR values (1.95). Administration of ACOLE had no significant influence (P>0.05) on immunological indices of broiler chickens for day 0 and day 42 but there was significant difference on day 12. The immunity (3.00 – 4.00) of the birds increased as ACOLE inclusion levels decreased. ACOLE had effect (P<0.05) on the histopathologicalindices observed in this study, ACOLE had some considerable level of protective effect on the liver with no negative influence on the intestine except for those on T4, (15mL/L ACOLE) where atrophy of villi, necrosis and cystic dilation of crypts was observed. Conclusively, the administration of 5mL/L ACOLE had the best FCR and posed a considerable level of protective effect on the liver sampled across the treatment groups.

Aqueous Chromolaena Odorata Leaf Extracts Induce Rice Resistance against Bacterial Leaf Blight

Aqueous Chromolaena odorata leaf extracts enhanced rice seed germination and seedling growth. Five extract concentrations (1, 2, 3, 4, and 5%, w/v) applied as seed soaking and foliar spraying were tested for their disease-reducing effects against rice bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae. For foliar sparying, the extracts were applied using three different methods, i.e., at 7 days before inoculation, at 14 days before inoculation and their combination. It was efficient to apply the 3% extract by seed soaking and foliar spraying at 14 days before inoculation to control BLB as it was the lowest concentration where the disease-reducing effects were observed until 21 DAI. The effects involved induced resistance. Indeed, activities of the four defense-related and antioxidant enzymes, i.e., peroxidase (POX), catalase (CAT), polyphenol oxidase (PPO), and phenylalanine ammonia lyase (PAL), increased after extract application and reached higher levels with both extract application and pathogen inoculation. Activities of POX and CAT were induced earlier and stronger using seed soaking than foliar spraying while those of PPO and PAL increased earlier using foliar spraying than seed soaking. This suggests a combination of both application methods to obtain coordinate increases in activities of the four defense-related and antioxidant enzymes which could provide sufficiently protection to rice plants again the disease.

Investigating the therapeutic potential of Chromolaena odorata (Linnaeus) leaf extracts on bacteria from wound source; Structural guide-approach through docking study

Investigating the therapeutic potential of Chromolaena odorata (Linnaeus) leaf extracts on bacteria from wound source; Structural guide-approach through docking study

GC-MS Charaterization of Phytochemicals and Anti Microbial Properties of Chromolaena odorata Leaf Harvested from South Eastern Nigeria

GC-MS Charaterization of Phytochemicals and Anti Microbial Properties of Chromolaena odorata Leaf Harvested from South Eastern Nigeria

Green Approach to Corrosion Control of Mild Steel in H3PO4 Using Chromolaena odorata Leaves Extract: Optimization

Green Approach to Corrosion Control of Mild Steel in H3PO4 Using Chromolaena odorata Leaves Extract: Optimization

FORMULATION DEVELOPMENT AND IN VITRO PENETRATION TEST OF ETHOSOME OF CHROMOLAENA ODORATA LEAVES EXTRACT

Objective: This study aimed to develop and assess ethosome preparation using extracts derived from the leaves of Chromolaena odorata. Methods: The study started by obtaining Chromolaena odorata leaf extracts. Furthermore, Ethosome formulations were produced using a thermal technique. Ethosome variants were created, each with distinct compositions: Formulation F1, containing 10 ml of ethanol without the extract; Formulation F2, consisting of 0.5 grams of the extract mixed with 10 ml of ethanol; Formulation F3, combining 1 gram of the extract with 20 ml of ethanol; and Formulation F4, incorporating 1.5 grams of the extract with 30 ml of ethanol. The ethosomal systems were thoroughly characterized using various analytical techniques, such as organoleptic analysis, quantification of particle dimensions, zeta potential evaluation, pH metric analysis, transmission electron microscopy (TEM) imaging, and in vitro permeability assessment using the Franz Diffusion Cell apparatus. Results: The findings indicated that the optimized F4 formulation showed 161.2±32.0 nm particle size measurement and a+34.33±0.58 mV zeta potential. All formula possess a pH range of 4.5-6.5, within which the skin can acclimate to preparations. It is evident from all formulations that the pH decreased after the addition of the extract at an acidic pH of 4.11. Following the 12-week storage period, the pH of all treatments exhibited a modest reduction; however, it remained within the acceptable range for skin pH. Furthermore, the F4 formula also had a higher level of penetration activity. Conclusion: The optimized ethosomal formulations of Chromolaena odorata have promising applications in enhancing the permeability and efficacy of plant-derived therapeutic agents.

Hematological Effects of Chromolaena Odorata Leaves Extract on Phenyl Hydrazine Induced Anaemic Rats

Hematological Effects of Chromolaena Odorata Leaves Extract on Phenyl Hydrazine Induced Anaemic Rats

The Potential of Processing Chromolaena Odorata Leaves in Solving Health Issues: A Review

Chromolaena Odorata L. locally called kopasanda, is an invasive plant native to Indonesia that belongs to the Asteraceae family. Traditionally, this plant is commonly consumed and processed into several foods used to treat various health problems. This study aims to review the food potential of Chromolaena Odorata L. in overcoming health problems. This literature study was obtained from analysis of library sources such as Google Scholar, PubMed, and Science Direct. The results show that Chromolaena Odorata L. could be processed into food or drink used to treat health problems such as wound healing, skin infections, stomach problems, and others. The benefit is due to the plant's chemical compounds, which are beneficially used as antibiotic, anti-inflammatory, antipyretic, antioxidant, analgesic, and other properties. The conclusion is that the processed Chromolaena Odarata L. plant has the potential to be used as a treatment for several health problems.

Phytochemical and Antibacterial Screening of Chromolaena odorata Leaf Extract

The discovery of antibacterial drugs from natural sources as a substitute for preventing bacterial resistance has become necessary due to the increase in bacterial drug resistance. Chromolaena odorata has been reported to have antibacterial effects. Therefore, this study investigated the phytochemical components and antibacterial properties of C. odorata leaves. C. odorata leaves were collected, prepared, and extracted using a standard procedure with 95% methanol and 95% ethanol, respectively. Both extracts were subjected to phytochemical screening and Gas Chromatography-Mass Spectrometry to detect phytochemical compounds. Chromolaena odorata methanolic extract at a concentration of 50 mg/mL, 100 mg/mL, 150 mg/mL and 200 mg/mL was carried out for antibacterial screening using the agar well diffusion tested on Staphylococcus aureus (ATCC 43330), Bacillus subtilis (B29), Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 15442). The methanolic extract was present with tannin, terpenoid, alkaloid, and saponin while ethanolic extract was present with tannin and alkaloid. The GC-MS chromatogram identified as many as 27 and 19 compounds for ethanolic and methanolic extract, respectively. The C. odorata ethanolic extract consists mainly of Phenol (0.51%), Sesquiterpenoid (20.69%) and Fatty acid (39.2%). Meanwhile, C. odorata methanolic extract consists of Sesquiterpenoid (62.26%) and Fatty acid (17.11%). Antibacterial activity of S. aureus was the highest inhibition zone diameter with 7.67 mm to 10.33 mm from 50 mg/ml to 200 mg/mL concentration of C. odorata extract. Escherichia coli and B. subtilis inhibition zones diameter was 200 mg/mL concentration of C. odorata extract. No antibacterial activities were obtained for P. aeruginosa. This study suggests that C. odorata consists of a variety of phytochemicals and contains antibacterial properties. Thus, C. odorata represents a promising natural source for antibacterial resistance drugs.

ELECTROCHEMICAL PERFORMANCE OF ZINC OXIDE NANOPARTICLES PREPARED VIA GREEN SYNTHESIS ROUTE USING CHROMOLAENA ODORATA LEAVES EXTRACT AS POTENTIAL ANODE MATERIAL IN SODIUM-ION BATTERY

A paradigm shift in the preparation of nanoparticles from a chemical synthetic route to the green synthetic method using plant extractives has gained much impetus. This study used Chromolaena odorata leaf extract to prepare zinc oxide nanoparticles (ZnO-NPs). The ZnO-NPs were characterised by spectroscopic, optical and thermal techniques. Thermogravimetric Analysis (TGA) revealed no significant weight loss observed after 550°C for precursor powder which was further calcined using a furnace at 800°C and 900°C to obtain ZnO-NPs. Fourier Transform Infrared Spectroscopy (FTIR) spectroscopy analysis indicated Zn-O stretching between 400 cm-1 to 500 cm-1. UV-Vis spectra show a strong absorption band at 388 nm for both calcination temperatures. Scanning Electron Microscopy (SEM) images showed the irregular shapes of the samples at different calcinations. The X-ray diffraction (XRD) diffractograms showed that the synthesised ZnO-NPs are in a hexagonal wurtzite structure. Studies of the electrochemical performance showed that ZnO-NPs made with Chromolaena odorata extract and calcined at 800oC have better cyclability than ZnO-NPs calcined at 900oC. Due to better cyclability, these ZnO-NPs can be applied as anode material in sodium-ion batteries.

Antibiogram and antimicrobial potency of Chromolaena Odorata leaf crude extracts on post-operative wound bacterial flora from hospital settings

Antibiogram and antimicrobial potency of Chromolaena Odorata leaf crude extracts on post-operative wound bacterial flora from hospital settings

Investigation of Phytochemicals and Antibacterial Activity of Persicaria odorata (L.) Methanolic Leaf Extract Against Selected Bacteria

Multidrug-resistant microorganisms pose serious threats, especially to children from developing countries. Due to this concern, there is a greater need to develop potentially effective antibacterial agents from natural resources. This study aimed to investigate the phytochemical compounds of Persicaria odorata (L.) methanolic leaf extract and its antibacterial activity against selected bacteria. Agar well diffusion and broth microdilution were used to test gram-positive (Staphylococcus aureus and Bacillus cereus) and gram-negative bacteria (Salmonella enterica serovar typhimurium and Shigella flexneri). The bacteria were treated with P. odorata (L.) leaves extracted using methanol and diluted with 10% Dimethyl-sulfoxide (DMSO). P. odorata (L.) leaf extract was highly effective against S. aureus at a concentration of 1,000 mg/mL. Gram-positive bacteria had the lowest MIC and MBC values. The qualitative phytochemical analysis of the methanolic extract of P. odorata (L.) leaves indicated the presence of antibacterial compounds such as phenols, flavonoids, terpenoids, and tannins. In conclusion, P. odorata (L.) leaves which have been widely used in cuisines and traditional medicine also possess the potential to serve as an antibacterial agent.

Evaluation of phytochemical constituents and antibacterial efficacy of Chromolaena odorata (Linnaeus) leaf extracts on bacteria associated with patient’s wounds

Evaluation of phytochemical constituents and antibacterial efficacy of Chromolaena odorata (Linnaeus) leaf extracts on bacteria associated with patient’s wounds

Toxicity Effects of Kirinyuh Leaf Ethanol Extract (Chromolaena Odorata L.) on White Rats (Rattus Novergicus)

Kirinyuh leaves (Chromolaena odorata L.) are natural medicinal plants for wound healing. WHO (World Health Organization) recommends and concern about the safety of plant medicines. Due to limited toxicity study of Chromolaena odorata, this study aimed to determine the acute toxicity of the ethanolic extract of C. odorata in terms of LD50 and its effect on liver and kidney function of male white rats. The observed toxicity parameters were LD50 and delayed toxic effects for 14 days, including toxic symptoms, body weight, AST, ALT and creatinine test, liver and kidney histopathology. The study used 24 white male rats divided into 4 groups, namely the control group NaCMC 0.5%, the treatment group of C. odorata leaf ethanol extract 5 g/kg bw, 10 g/kg bw and 15g/kg bw with a single dose. The results showed that no white rats died during the 14 days of observation. Toxic symptoms that appear only white rats lack of appetite. Other parameter was body weight between days 1, 3, 7, 10 and 14 for each treatment group was significantly different and tended to decrease. The AST, ALT and creatinine values ??were not significantly different for all treatments. Histopathology of the liver and kidneys showed no abnormalities. Based on these results, the LD50 of kirinyuh leaf extract was 15 g/kg bw and delayed toxic effects in the form of lack of appetite which caused weight loss in all treatment groups.

Potensi Oleoresin Gulma Daun Siam (Chromolaena odorata) pada Mortalitas Hama Lalat Buah (Bactrocera cucurbitae) pada Uji in-vitro

Fruit flies are the main pests that attack various types of fruit vegetables and fruits that can caused a damage in the quality and quantity of production. Fruit flies controlling by chemical insecticides is common to use nowadays, but excessive use of chemical insecticides has a negative impact on the health and environment. One alternative to control the fruit flies is using oleoresins of Chromolaena odorata leaf. This study aims to determine the effect of C. Odorata leaf oleoresin on increasing the mortality of fruit fly pests (Bactrocera cucurbitae). This research used an experimental method with Completely Randomized Design (CRD) which had 6 treatment levels, namely: N0 (Aquadest), N1 (Oleoresins 2%), N2 (Oleoresins 4%), N3 (Oleoresins 6%), N4 (Oleoresins 8%), N5 (Oleoresins 10%), was replicated 4 times so there were 24 experimental units. The results showed that the application of C. Odorata leaf oleoresin had no sigificant effect on mortality percentage and mortality rates, but oleoresin had the potential to increasing fruit fly mortality as concentrations increased. It is necessary to test the increasing concentration of oleoresin > 10% to control fruit fly pests.

Anti-Ulcer Effect on Indomethacin-Induced Ulcerated Mice of Chromolaena odorata Leaf from Vietnam and its Secondary Metabolites

Chromolaena odorata (C. odorata) (L.) R. King & H. Robinson is a perennial flowering shrub with diverse habitats, including crops, grasslands, and roadsides. This plant has been widely used in Vietnamese folk medicine for gastric ulcer treatment. Hence, the present study aimed to evaluate the acute toxicity and the anti-ulcer effect of the ethanol crude extract of C. odorata leaves and its fractions against the indomethacin-induced gastric ulcer model in mice, and investigate the chemical constituents of the most active fraction. According to in vivo results, the ethyl acetate residue with the highest anti-ulcer activity significantly reduced gastric lesions in theexperimental mice model with an ulcer index of 0.73 ± 0.39 and a percentage inhibition of 26.92%. Thus, this fraction was chosen for further chemical investigation. Four pure compounds (1-4) were extracted and isolated by using chromatographic methods. Based on the nuclear magnetic resonance spectroscopy, melting temperature, mass spectrometry analysis, and compared with thepublished literature, their structures were elucidated as 1-(4-hydroxy-3-methoxyphenyl)propane- 1,2-diol (1), kaempferol-7-O-α-L-rhamnopyranoside (2), naringenin-5,7-di-O-β-Dglucopyranoside (3), and rubrosterone (4). To our best knowledge, all of these compounds were isolated for the first time from C. odorata leaves. These findings contribute to providing scientific evidence for the traditional use and phytochemicals of C. odorata leaves.&nbsp