Sensory evaluation of five different kinds of butter revealed an Irish sour cream butter (ISC) and a farmer sour cream butter (FSC) with the highest overall odour intensities. Nineteen odour-active compounds were detected by aroma extract dilution analysis in a distillate of the ISC butter. The highest flavour dilution (FD)-factors were found for δ-decalactone, skatole, [Z]-6-dodeceno-γ-lactone and diacetyl followed by [E]-2-nonenal, [Z,Z]-3,6-nonadienal, [Z]-2-nonenal and 1-octen-3-one. Odour activity values (OAV; ratio of concentration to odour threshold) were calculated from quantitative data determined by means of stable isotope dilution assays and from odour thresholds in oil. Diacetyl followed by δ-decalactone and butanoic acid showed the highest OAVs in the ISC butter and a cultured butter with creamy, sweet odours. In contrast, a sweaty, rancid odour predominated in the FSC butter in which butanoic acid showed the highest OAV. The odour of a mixture of diacetyl (0.34mg/kg), δ-decalactone (4.9 mg/kg) and butanoic acid (3.6 mg/kg) in sunflower oil was in very good agreement with the odour of the cultured butter containing the same amounts of these odorants. The data suggest that the three odorants should be useful as indicators for the objective assessment of the buttery odour note in milkfat-containing products.