Abstract Purpose: The validity of the safety standard (10‐300 GHz 10 W/m2 in public, ICNIRP) of a wide‐ranging quasi‐ and millimeter‐ wave was examined from ocular temperature changes during exposure and ocular injury after exposure. Methods: Pigmented rabbits were exposed unilaterally to 18, 22, 26.5, 35, 40 GHz quasi‐ and millimeter‐wave with a lens antenna for 3 minutes. The focused beam was exposed to the center of the rabbit cornea. Ocular temperature changes (cornea, lens, vitreous, retrobulbar) during a 3 min. exposure were measured with a Fluoroptic thermometer and corneal surface temperature was measured with a thermography camera. For evaluating ocular injury, a rabbit was exposed to one frequency (18, 22, 26.5, 35, 40, 60 GHz) with 800, 1500, 3000 mW/cm2 for 6 min. Ocular changes were evaluated by slit lamp and ocular inflammation was measured by laser flare meter immediately after and 1 day after exposure. Enucleated lens was observed with lens epithelial cell flat mounting. Results: Cornea and lens temperature rise were detected by exposure with all examined frequencies. The highest ocular and corneal surface temperature was 40 GHz, followed by 35, 22 and 18 (almost the same), and the lowest was 26.5 GHz. Ocular injury by 800 mW/cm2 for 6 min. exposure was seen only with 40 GHz. Miosis and iris vasodilation were seen immediately after exposure, and corneal epithelial defect at the center of the cornea and corneal opacity were seen one day after exposure. 40 GHz 3000 mW/cm2 for 6 min. exposure developed cornea and lens opacity. But the same dose of 60 GHz exposure showed only corneal opacity. Conclusions: It was suggested that thermal and biological reactions differed according to frequency.