Octodon Degus Research Articles

Chromatic pupillometry for the characterization of the pupillary light reflex in Octodon degus

The common degu (Octodon degus) is an emerging model in biomedical science research due to its longevity and propensity to develop human-like conditions. However, there is a lack of standardized techniques for this non-traditional laboratory animal. In an effort to characterize the model, we developed a chromatic pupillometry setup and analysis protocol to characterize the pupillary light reflex (PLR) in our animals. The PLR is a biomarker to detect early signs for central nervous system deterioration. Chromatic pupillometry is a non-invasive and anesthesia-free method that can evaluate different aspects of the PLR, including the response of intrinsically photosensitive retinal ganglion cells (ipRGCs), the disfunction of which has been linked to various disorders. We studied the PLR of 12 degus between 6 and 48 months of age to characterize responses to LEDs of 390, 450, 500, 525 and 605 nm, and used 5 with overall better responses to establish a benchmark for healthy PLR (PLR+) and deteriorated PLR (PLR-). Degu pupils contracted up to 65% of their horizontal resting size before reaching saturation. The highest sensitivity was found at 500 nm, with similar sensitivities at lower tested intensities for 390 nm, coinciding with the medium wavelength and short wavelength cones of the degu. We also tested the post-illumination pupillary response (PIPR), which is driven exclusively by ipRGCs. PIPR was largest in response to 450 nm light, with the pupil preserving 48% of its maximum constriction 9 s after the stimulus, in contrast with 24% preserved in response to 525 nm, response driven mainly by cones. PLR- animals showed maximum constriction between 40% and 50% smaller than PLR+, and their PIPR almost disappeared, pointing to a disfunction of the iPRGCs rather than the retinal photoreceptors. Our method thus allows us to non-invasively estimate the condition of experimental animals before attempting other procedures.

Diurnal rodents as pertinent animal models of human retinal physiology and pathology.

This presentation will survey the retinal architecture, advantages, and limitations of several lesser-known rodent species that provide a useful diurnal complement to rats and mice. These diurnal rodents also possess unusually cone-rich photoreceptor mosaics that facilitate the study of cone cells and pathways. Species to be presented include principally the Sudanian Unstriped Grass Rat and Nile Rat (Arvicanthis spp.), the Fat Sand Rat (Psammomys obesus), the degu (Octodon degus) and the 13-lined ground squirrel (Ictidomys tridecemlineatus). The retina and optic nerve in several of these species demonstrate unusual resilience in the face of neuronal injury, itself an interesting phenomenon with potential translational value.

Chromatic pupillometry: Characterization of the pupillary light reflex in Octodon degus

Chromatic pupillometry: Characterization of the pupillary light reflex in Octodon degus

Voluntary exercise reduces plasma cortisol levels and improves transitory memory impairment in young and aged Octodon degus

Sleep deprivation (SD) has been reported to induce transient cognitive impairment in functional domains commonly affected in dementia, including memory. Indeed, sleep disturbance has been proposed as an early marker for Alzheimer’s disease (AD). SD emulates many aging-related modifications, including important memory dysfunctions possibly caused by triggers of stress such as cortisol. Although exercise is widely assumed to be beneficial for overall health, only recently has the research community focused its attention on its possible effects on brain functions such as cognition. Octodon degus (O. degus) is a recent rodent model considered suitable for the study of neurodegenerative diseases, since it spontaneously develops several histopathological hallmarks observed in AD. We aimed to uncover the interaction between stress, exercise, age and transient memory impairments after SD insult. In this study, animals had free individual access to wheels to practice voluntary exercise. The Barnes Maze (BM) task was conducted with young and aged O. degus animals after combining voluntary exercise and either normal sleep or SD. Plasma cortisol levels were measured after each condition. SD impaired hippocampus-dependent memory in both young and old animals, while cortisol levels did not significantly differ between non-SD and SD animals. However, voluntary exercise for 45 days improved the cognitive impairment caused by SD compared with the control condition. Moreover, voluntary exercise decreased plasma cortisol levels in both conditions, independently of the age.

Response to lipopolysaccharide in Octodon degus pups produces age-related sickness behavior but does not have effects in juveniles.

During vertebrate development, the immune function is inefficient and is mainly controlled by innate defense. While there have been detailed studies of various aspects of innate immune function, the effects of this function in the growth of vertebrates is still not well known. Similarly, there is little information regarding how early endotoxin exposure would affect juvenile phenotypes, specifically in a non-model mammal like a precocial rodent. We evaluated the response to an antigen and its cost in offspring of the rodent Octodon degus. We inoculated pups at 4 different ages (8, 15, 22 and 30 days after birth) with an antigen to determine the ontogeny and costs of the response to an endotoxin. We assessed changes in body mass, body temperature, sickness behavior and the levels of a key mediator of the inflammatory response, the cytokine interleukin-1β. We also determined the effects of early endotoxin exposure on the resting metabolic rate of juvenile animals (i.e. 90 days after birth). The cytokine levels, body mass and body temperature were unaffected by time of inoculation and treatment. However, pups subjected to inoculation at 22 days after birth with the antigen showed reduced locomotion. Juvenile resting metabolic rate was not affected by early endotoxin exposure. These results suggest that the magnitude of O. degus responses would not change with age. We discuss whether the lack of effect of the response on body mass or body condition is caused by environmental variables or by the precocial characteristics of O. degus.

Influence of Pre-Weaning Social Isolation on Post-Weaning Emotion Tendency and Mother–Infant Interactions in Infant Octodon Degus

Our previous research using Octodon degus (degus) revealed that preweaning social isolation negatively affected object exploratory behavior. However, it remains unknown how social isolation affects animal psychology and other behaviors. The present study examined the effects of neonatal social isolation on degu emotion and mother–infant interactions before and after weaning. Because degus have a complex social repertoire, we predicted that they would be sensitive to social isolation and show similarities with humans in their social interaction. Pups in the isolation group were separated from their family seven times for 30 min a day from 8 to 15 days post-birth. Pups in the nonisolation group were reared with their family. At 2, 3, 4, 5, and 6 weeks of age, pups underwent a zero-maze test to measure anxiety and a mother–infant interaction test to assess mother–infant attachment. Isolated pups showed more activity in the zero-maze test than nonisolated pups at 3 weeks of age. We found no significant effects of social isolation on mother–infant interactions. These results suggest that while neonatal social isolation might affect emotion during weaning, it does not influence mother–infant relationships.

Helminthic infection in three native rodent species from a semiarid Mediterranean ecosystem.

Helminth parasites are still not a well-known component of animal biodiversity. In this study, we describe and compare the endoparasite assemblages of three native rodent species from a semiarid Mediterranean ecosystem. A total of eight species of gastrointestinal helminth parasites were detected. The endoparasitic communities of the rodents Phyllotis darwini (5 species), Octodon degus (4 species), and Abrothrix olivaceus (3 species) were described in terms of their prevalence and mean abundance. In addition, endoparasite records by rodent species were submitted to rarefaction analyses to verify sampling robustness and used to compare richness and similarity among rodent species. O. degus presented the highest prevalence and parasitic load, but was of low diversity, while P. darwini presented the highest diversity, but had a low prevalence rate. Conversely, A. olivaceus presented the lowest prevalence and diversity. This study contributes to the existing knowledge of the endoparasitic fauna of rodent species from semiarid Mediterranean zones.

Mandible strength and geometry in relation to bite force: a study in three caviomorph rodents.

The monophyletic group Caviomorpha constitutes the most diverse rodent clade in terms of locomotion, ecology and diet. Caviomorph species show considerable variation in cranio-mandibular morphology that has been linked to the differences in toughness of dietary items and other behaviors, such as chisel-tooth digging. This work assesses the structural strength of the mandible of three caviomorph species that show remarkable differences in ecology, behavior and bite force: Chinchilla lanigera (a surface-dwelling species), Octodon degus (a semi-fossorial species) and Ctenomys talarum (a subterranean species). Finite element (FE) models of the mandibles are used to predict the stresses they withstand during incisor biting; the results are related to invivo bite forces and interspecific variations in the mandibular geometries. The study concludes that the mandible of C.talarum is better able to withstand strong incisor bites. Its powerful adducting musculature is consistent with the notorious lateral expansion of the angular process and the masseteric crest, and the enhanced cortical bone thickness. Although it has a relatively low bite force, the mandible of O.degus also shows a good performance for mid-to-strong incisor biting, in contrast to that of C.lanigera, which exhibits, from a mechanical point of view, the worst performance. The mandibles of C.talarum and O.degus appear to be better suited to withstand stronger reaction forces from incisor biting, which is consistent with their closer phylogenetic affinity and shared digging behaviors. The contrast between the low invivo bite force of C. lanigera and the relatively high estimations that result from the models suggests that its adductor musculature could play significant roles in functions other than incisor biting.

Multiple mating is linked to social setting and benefits the males in a communally rearing mammal

Abstract Individuals in social species may mate with multiple opposite-sex individuals, including members of the same or different social groups. This variation may be linked to genetic benefits, where multiple mating decreases risk of inbreeding. Multiple mating may also be constrained by the sociospatial setting through its effect on availability of mates. Because multiple mating with individuals from same or different groups may determine sex-specific fitness effects, we also examined how multiple mating modulates social benefits of females and males. We used 7 years of data on demography, social organization, and genetics of a natural population of the group-living and colonial rodent, Octodon degus, to determine how kin and sex composition within social groups, and spatial relations between these groups (i.e., colonial habits) influence multiple mating and its fitness consequences. Males (81.3%) and females (64.9%) produced offspring with multiple opposite-sex individuals within groups and with individuals of neighboring groups. Thus, polygynandry was the dominant mating system in the degu population examined. Multiple mating in degus was high when compared with estimates reported in other social mammals. Variation in female and male multiple mating was better explained by social setting through its effect on availability of potential mates rather than by benefits derived from decreasing risk of inbreeding. Finally, our study revealed how multiple mating enhances male, but not female reproductive success.

Male group members are costly to plurally breeding Octodon degus females

Abstract We report the results of a 6-year study of social (number of adult males/females, relatedness of females, communal litter size) and ecological (mean/CV of food abundance, soil hardness, burrow openings) factors influencing the direct fitness of plurally breeding degu (Octodon degus) females. The best fit models for per capita offspring weaned and standardized variance in direct fitness (within-group variation) included the number of adult males per group. Per capita number of offspring weaned decreased and standardized variance in direct fitness increased with increasing number of adult males per group. Thus, females experience a cost associated with males that is not shared equally. Standardize variance in direct fitness decreased with increasing communal litter size. All other factors were not significant predictors of direct fitness variation. Our study suggests that plural breeding may not be as egalitarian as previously thought. Consequences of plural breeding may be influenced by intra- and inter-sexual conflict.

Characterization of Retinal Functionality at Different Eccentricities in a Diurnal Rodent.

Although the properties of the neurons of the visual system that process central and peripheral regions of the visual field have been widely researched in the visual cortex and the LGN, they have scarcely been documented for the retina. The retina is the first step in integrating optical signals, and despite considerable efforts to functionally characterize the different types of retinal ganglion cells (RGCs), a clear account of the particular functionality of cells with central vs. peripheral fields is still wanting. Here, we use electrophysiological recordings, gathered from retinas of the diurnal rodent Octodon degus, to show that RGCs with peripheral receptive fields (RF) are larger, faster, and have shorter transient responses. This translates into higher sensitivity at high temporal frequencies and a full frequency bandwidth when compared to RGCs with more central RF. We also observed that imbalances between ON and OFF cell populations are preserved with eccentricity. Finally, the high diversity of functional types of RGCs highlights the complexity of the computational strategies implemented in the early stages of visual processing, which could inspire the development of bio-inspired artificial systems.

Ecoimmunology in degus: interplay among diet, immune response, and oxidative stress

The relationships between immunity, oxidative stress, and diet have not often been studied together. Despite this, it has been shown that dietary proteins can have effects on the functioning of the immune system and the oxidative status of animals. Here we evaluated the effects of dietary proteins on the response to an antigen and oxidative status of Octodon degus (Rodentia). We acclimated adult individuals to high-protein and low-protein diets and evaluated several aspects of the acute phase response and variables associated with oxidative status. After the immune challenge, animals acclimated to the high-protein diet had more inflammatory proteins and body mass losses than the group acclimated to a low-protein diet. Overall, the immune challenge increased the production of inflammatory proteins, total antioxidant capacity, lipid peroxidation, and duration of rest periods. In contrast, we did not find an interaction between diet and the challenge with the antigen. Overall, our results do not reveal an enhanced response to an antigen nor effects on the oxidative status of degus individuals subjected to a high-protein diet.

New Insights into the Spontaneous Human Alzheimer's Disease-Like Model Octodon degus: Unraveling Amyloid-β Peptide Aggregation and Age-Related Amyloid Pathology.

Alzheimer's disease (AD) is the most common cause of dementia worldwide. Despite advances in our understanding of the molecular milieu driving AD pathophysiology, no effective therapy is currently available. Moreover, various clinical trials have continued to fail, suggesting that our approach to AD must be revised. Accordingly, the development and validation of new models are highly desirable. Over the last decade, we have been working with Octodon degus (degu), a Chilean rodent, which spontaneously develops AD-like neuropathology, including increased amyloid-β (Aβ) aggregates, tau hyperphosphorylation, and postsynaptic dysfunction. However, for proper validation of degu as an AD model, the aggregation properties of its Aβ peptide must be analyzed. Thus, in this study, we examined the capacity of the degu Aβ peptide to aggregate in vitro. Then, we analyzed the age-dependent variation in soluble Aβ levels in the hippocampus and cortex of third- to fifth-generation captive-born degu. We also assessed the appearance and spatial distribution of amyloid plaques in O. degus and compared them with the plaques in two AD transgenic mouse models. In agreement with our previous studies, degu Aβ was able to aggregate, forming fibrillar species in vitro. Furthermore, amyloid plaques appeared in the anterior brain structures of O. degus at approximately 32 months of age and in the whole brain at 56 months, along with concomitant increases in Aβ levels and the Aβ42/Aβ40 ratio, indicating that O. degus spontaneously develops AD-like pathology earlier than other spontaneous models. Based on these results, we can confirm that O. degus constitutes a valuable model to improve AD research.

The nucleus pretectalis principalis: A pretectal structure hidden in the mammalian thalamus.

A defining feature of the amniote tecto-fugal visual pathway is a massive bilateral projection to the thalamus originating from a distinct neuronal population, tectal ganglion cells (TGCs), of the optic tectum/superior colliculus (TeO/SC). In sauropsids, the thalamic target of the tecto-fugal pathway is the nucleus rotundus thalami (Rt). TGCs axons collateralize en route to Rt to target the nucleus pretectalis principalis (PT), which in turn gives rise to bilateral projection to the TeO. In rodents, the thalamic target of these TGCs afferents is the caudal division of the pulvinar complex (PulC). No pretectal structures in receipt of TGC collaterals have been described in this group. However, Baldwin et al. (Journal of Comparative Neurology, 2011;519(6):1071-1094) reported in the squirrel a feedback projection from the PulC to the SC. Pulvino-tectal (Pul-T) cells lie at the caudal pole of the PulC, intermingled with the axonal terminals of TGCs. Here, by performing a combination of neuronal tracing, immunohistochemistry, immunofluorescence, and in situ hybridization, we characterized the pattern of projections, neurochemical profile, and genoarchitecture of Pul-T cells in the diurnal Chilean rodent Octodon degus. We found that Pul-T neurons exhibit pretectal, but not thalamic, genoarchitectonical markers, as well as hodological and neurochemical properties that match specifically those of the avian nucleus PT. Thus, we propose that Pul-T cells constitute a pretectal cell population hidden within the dorsal thalamus of mammals. Our results solve the oddity entailed by the apparent existence of a noncanonic descending sensory thalamic projection and further stress the conservative character of the tectofugal pathway.

Comparing vector competence of Mepraia gajardoi and Triatoma infestans by genotyping Trypanosoma cruzi discrete typing units present in naturally infected Octodon degus

Chagas disease is a vector-borne disease caused by the parasite Trypanosoma cruzi, and transmitted by triatomine insects to several mammal species. In Chile, the wild triatomine species are the endemic Mepraia species, and the only domestic vector of Chagas disease is Triatoma infestans. The aim of this study was to determine the competence of M. gajardoi compared to T. infestans as a T. cruzi vector using the naturally infected rodent Octodon degus. M. gajardoi amplified T. cruzi present in all O. degus studied while T. infestans only in half of the infected rodents. Both triatomine species excrete metacyclic trypomastigotes and amplified the same three T. cruzi DTUs, however, M. gajardoi showed differences in their ability to amplify TcI. TcV and TcVI had the same probability to be amplified by both triatomine species. Both species amplified mixed infections, with TcI-TcVI as the most represented. This study reports the higher vector competence of M. gajardoi in comparison to T. infestans.

The effects of pre-weaning social isolation on emotion of infant <i>Octodon Degus</i>

The effects of pre-weaning social isolation on emotion of infant <i>Octodon Degus</i>

Frugivory and seed dispersal in the endemic cactus Eulychnia acida: extending the anachronism hypothesis to the Chilean Mediterranean ecosystem

BackgroundEulychnia acida is an endemic Chilean cactus species whose fruits show several traits that, taken as a whole, are compatible with a seed dispersal syndrome by large herbivore vertebrates. Since only a few large native mammals exist in Chile at present, cactus fruit consumption and seed dispersal may be coopted by introduced mammals as predicted by Janzen and Martin’s (1982) hypothesis for tropical ecosystems.FindingsWe describe the current frugivore species of E. acida in a protected semiarid-Mediterranean ecosystem using field measurements and feeding experiments. In addition, to examine a potential role as seed dispersers of the cactus species, we offered fruits and performed germination tests on seeds defecated by Lama guanicoe and the introduced goat Capra a. hircus under captivity conditions. Our data indicate that while fruits of E. acida are pecked by the Chilean tinamou, Nothoprocta perdicaria, and the Chilean mockingbird, Mimus thenca, and eaten by the brush-tailed rodent, Octodon degus, none of these species could be considered a legitimate seed disperser. Unlike L. guanicoe, the goat C. a. hircus did not reduce seed germination, having a neutral effect.ConclusionsResults from this study indicate that introduced C. a. hircus was the only species showing a potential role in the seed dispersal process of E. acida. In the absence of native frugivore species, goats might play an important role in population recruitment of the endemic cactus. These results extend Janzen and Martin’s (1982) anachronism hypothesis to the Chilean Mediterranean ecosystem.

EVALUATION OF ORCHIECTOMY AND OVARIECTOMY SURGICAL TECHNIQUES IN DEGUS (OCTODON DEGUS)

In this study, 2 previously described surgical techniques for performing an orchiectomy in the adult male degu (Octodon degus) were evaluated: (1) prescrotal open technique and (2) scrotal open technique. Additionally, an ovariectomy procedure was assessed in female degus using the bilateral (dorsolateral) technique similar to that performed in related species. Twenty two animals (n = 14 males; 8 females) from a local small mammal shelter group, that were presented for routine surgical castration for population control measures, were included in this study. There was a significant difference in mean surgical time for the prescrotal and scrotal approach of 16.46 ± 4.28 and 23.83 ± 2.09 minutes, respectively. No significant differences were noted on anesthesia and recovery times for the orchiectomy procedures. Overall, the prescrotal approach was a faster procedure and required less anesthesia time. One male degu undergoing the scrotal open technique died during surgery, this was believed to be related to anesthesia complications (increased saliva secretions). No other complications were reported perioperatively in any other animals. All animals were observed to have sufficient pain management using an in-house visual analogue scale. The bilateral flank (dorsolateral) ovariectomy approach in female degus yielded a short total procedure time with no adverse events. No over grooming and or self-injurious behaviors were reported during both the 24 hours and 14 days recheck examinations.

CHORDOMA OF THE TAIL IN A DEGU (OCTODON DEGUS)

A 6-year-old female degu (Octodon degus) in good body condition was presented with a mass at the base of the tail. Following presentation and the owners’ approval, the tail of the degu was surgically amputated. The mass was submitted for pathological evaluation following the surgical procedure. Histologically, the mass was comprised of lobules and sheets of polygonal cells displaying moderate amounts of basophilic foamy cytoplasm (physaliferous cells). The pathological results of the tail mass were consistent with chordoma. Approximately 3 months following the tail amputation the patient died.

Ectoparasites of the endemic rodent Abrocoma bennetti (Hystricomorpha: Abrocomidae) from semiarid Chile

A total of 13 individuals of the Abrocoma bennetti rodent were captured and 354 ectoparasites belonging to 10 different species were collected and analyzed. The most abundant species was a Phithraptera, Monogyropus longus (62%), followed by Siphonaptera Delostichus smiti (23%). In this study Gyropus distinctus (15%) is reported as new record for A. bennetti, previously described for Octodon degus, a rodent usually living in sympatry with A. bennetti. For the first time the mites Ornithonyssus sp. and Androlaelaps fahrenholzi are recorded in this rodent species.