Ocimum Sanctum Research Articles

Essential Oil and Waste Hydrosol of Ocimum Tenuiflorum L.: A Low-Cost Raw Material Source of Eugenol, Botanical Pesticides, and Therapeutic Potentiality.

In this study, essential oils and waste hydrosols of leaves of Ocimum tenuiflorum in four different geographical locations were extracted by hydrodistillation method and using gas chromatography/mass spectrometry (GC/MS) for chemical composition analysis. All four essential oil samples contained the main components (E)-β-caryophyllene (27.8-49.0 %), trans-β-elemene (20.3-37.1 %) and eugenol (9.0-44.0 %). Three of the four hydrosol samples had eugenol in absolute content (94.5-98.6 %), while the remaining hydrosol sample had two main components, elemicin (77.8 %) and eugenol (14.2 %). Essential oils and hydrosols demonstrated larvicidal activities against four important disease-transmitting mosquito species including Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Culex fuscocephala with 24-h LC50 values in the range 15.42-56.01 μg/mL and 53.88-97.80 μg/mL for the essential oils and the hydrosols, respectively. Essential oils and hydrosols strongly inhibited the acetylcholinesterase (AChE) enzyme of electric eels with IC50 values in the range of 25.35-107.19 μg/mL. Microemulsion (ME) can be considered as a sustainable pesticide formulation over 300 days and has improved larvicidal activity compared to free essential oil. The O. tenuiflorum in Vietnam can be considered a low-cost source of eugenol, botanical pesticides that control disease-transmitting mosquitoes, as well as having therapeutic potential to be further investigated.

Antimicrobial Effect of Medicinal Plants Against Streptococcus mutans: A Literature Review

The present study aims to evaluate, through a literature review, the antimicrobial potential of medicinal plants against Streptococcus mutans. Method: A bibliographic search was conducted in the PubMed database using the descriptors "phytotherapy," "medicinal plants," and "Streptococcus mutans," either combined or not, covering the period from 2011 to 2021. The search resulted in 99 articles. In the initial screening, 79 articles were excluded for not being related to the topic. Twenty articles were selected, but only 12 met the eligibility criteria, which included studies evaluating the antimicrobial effect of a single medicinal plant on S. mutans only. Among the results, several plant species with antimicrobial potential against S. mutans were identified, including Annona senegalensis, Melia azedarach, Copaifera langsdorffii, Copaifera officinalis, Zingiber zerumbet, Terminalia chebula, Trachyspermum ammi, Ocimum sanctum, Teucrium polium, Vitis vinifera L., and Cinnamomum zeylanicum Blume. In summary, all studies showed antimicrobial effects against S. mutans, with particular emphasis on the studies with copaiba oil, M. azedarach crude extract, and T. polium, which demonstrated superior and comparable results to the effects of chlorhexidine, respectively.

Influence of diurnal harvesting times on essential oil yield and chemical composition of lemongrass, palmarosa and tulsi

Essential oil yield and chemical composition of most of the aromatic crops are affected by environmental, agrochemical and genetic factors. One of such factors are Harvesting Time (HT’s), optimizing which may result in higher essential oil production with better quality. The present study was carried out to determine the effect of HT’s on three different aromatic crops [lemongrass, palmarosa and Ocimum sanctum (CIM-Ayu)] at different diurnal times (9.00 A.M 12.00 P.M and 3.00 P.M) in a day and the same was repeated thrice on alternate days from in the same fields. The results indicated that HT at 3.00 PM had higher essential oil content in all the three aromatic crops. But, the influence of HT on the quality of essential oil was specific to the different crops studied in this report. The quality of essential oil obtained from lemongrass harvested at 12.00 PM was found to be better as it showed more percentage of citral whereas the essential oils obtained from palmarosa and O. sanctum harvested at 3.00 PM were better as they showed more percentage of geraniol and eugenol respectively. This research not only refines essential oil production strategies but also underscores the crop-specific impact of harvesting times. The meticulous temporal analysis, coupled with statistical validation, contributes novel insights, providing practical benefits for industries reliant on these aromatic crops.

Exploring Holy Basil's Bioactive Compounds for T2DM Treatment: Docking and Molecular Dynamics Simulations with Human Omentin-1.

Type 2 Diabetes Mellitus (T2DM) presents a substantial health concern on a global scale, driving the search for innovative therapeutic strategies. Phytochemicals from medicinal plants, particularly Ocimum tenuiflorum (Holy Basil), have garnered attention for their potential in T2DM management. The increased focus on plant-based treatments stems from their perceived safety profile, lower risk of adverse effects, and the diverse range of bioactive molecules they offer, which can target multiple pathways involved in T2DM. Computational techniques explored the binding interactions between O. tenuiflorum phytochemicals and Human Omentin-1, a potential T2DM target. ADMET evaluation and targeted docking identified lead compounds: Luteolin (-4.84 kcal/mol), Madecassic acid (-4.12 kcal/mol), Ursolic acid (-5.91 kcal/mol), Stenocereol (-5.59 kcal/mol), and Apigenin (-4.64 kcal/mol), to have a better binding affinity to target protein compared to the control drug, Metformin (-2.01 kcal/mol). Subsequent molecular dynamics simulations evaluated the stability of Stenocereol, Luteolin, and Metformin complexes for 200 nanoseconds, analysing RMSD, RMSF, RG, SASA, PCA, FEL, and MM-PBSA parameters. Results indicated Stenocereol's strong binding affinity with Omentin-1, suggesting its potential as a potent therapeutic agent for T2DM management. These findings lay the groundwork for further experimental validation and drug discovery endeavours.

Intercalation of novel Ocimum Sanctum leaves derived carbon dots between g-C3N4/CoFe2O4 Z-scheme heterojunction system for boosting the radicals’ generation in photo-Fenton degradation and synchronized electrochemical sensing of sulfamethoxazole antibiotic

Deeming about incessant consumption and disposal of pharmaceutical antibiotics in natural water bodies, this study demonstrates the synthesis of a novel Tulsi leaves (Ocimum Sanctum) derived carbon dots modified Z-scheme g-C3N4/CoFe2O4 heterojunction system. Characterization techniques namely XRD, FT-IR, XPS, FE-SEM, HR-TEM and EDX supported composite formation. Fabricated catalysts presented excellent photocatalytic performance towards removal of sulfamethoxazole (SMX). UV–vis DRS, PL and EIS findings suggested the augmented visible light absorption capability, suppression of electron-hole pairs recombination and effective separation of charge carriers which were accountable for degradation process. Probable mechanistic pathway for SMX removal was photo-Fenton assisted with Z-scheme separated electron-hole pairs via activation of H2O2, where hydroxyl radicals (•OH) were main reactive species. Additionally, the prepared material was employed as electrochemical sensor for individual as well as simultaneous detection of SMX and trimethoprim with quite impressive detection limits of 0.042 µM and 0.047 µM, respectively.

Green synthesis of silver nanoparticle for catalytic applications and priming study by seed germination

Silver nanoparticles (AgNPs) have been successfully synthesized using leaf extract of Neem (Azadirachta Indica), Mint (Mentha Piperita), Tulsi (Ocimum Tenuiflorum), Bermuda grass (Cynodon Dactylon) and silver salt. As plant extracts produce best capping material for the stabilization of nanoparticles. AgNPs were characterized by UV–Vis spectroscopy in range of 200–800 nm and transmission electron microscopy TEM, XRD and FTIR. The nanoparticles synthesized were mainly in sizes between 25 and 100 nm. They appeared to be spherical, nanotriangles and irregular in shape. Catalytic application was observed for all the aqueous solution of leaves, quantity taken was 1 ml, 2 ml, 3 ml, 4 ml and 5 ml. Furthermore, prepared Ag nanoparticles are also used for seed germination.

Role of Indian Medicinal Plants for Immunity Booster Against SARS-CoV-2 Infection: An Updated Review

Abstract: The coronavirus disease (COVID-19) came to be an epidemic that has occurred at the expense of the health of people. This disease reveals it can be lethal. Aged people and people with other medical occurrences may be more susceptible and become remarkably ill. The existing pandemic situation mandates scientific vigilance, hence we exploration to medicinal plants like Curcuma longa, Azadirachta indica, Ocimum sanctum, Tinospora cordifolia, Triphala, Phyllanthus emblica, Aloe barbadensis, Syzygium aromaticum, Cinnamomum verum, Allium cepa, Zingiber officinale, Piper nigrum, Allium sativum. The holistic Indian therapeutic system prescription is acknowledged as “Ayurveda”. Natural herbal remedies show more effectiveness which is moving towards the present day with the help of traditional medicine. The factual of the Immunity booster is to determine components of natural lineage have an antiviral outcome. This booster helps to prevent humans from infection caused by SARS-CoV-2. The molecular docking technique is used to disclose the interaction between molecules which is already researched, and the protein. In conclusion, the identified natural compounds from medicinal plants act as an herbal booster for increasing immunity in contradiction to SARS-CoV-2 contagion in patients.

Green synthesized silver nanoparticles from Ocimum sanctum: A potent inhibitor of biofilm forming ability and efflux pumps in bacteria causing bovine mastitis

Therapeutic management of mastitis faces significant challenges due to multidrug resistance. In the present study, multi-drug-resistant (MDR) Staphylococcus spp, Klebsiella pneumoniae, and Escherichia coli were isolated from bovine clinical mastitis cases and the phenotypic and genotypic multidrug resistance profiling was carried out. Silver nanoparticles (AgNPs) were biosynthesized using Ocimum sanctum leaf extracts and characterized via UV Vis absorption, Fourier Transform Infrared Spectroscopy, X-ray diffraction studies, Energy dispersive spectroscopy and Electron Microscopy. The determined minimum inhibitory concentration and minimum bactericidal concentration of the AgNPs against the recovered MDR isolates were 62.5 μg/ml and 125 μg/ml respectively. At a concentration of 50 μg/ml, the AgNPs demonstrated biofilm inhibitory activities of 80.35 % for MDR E. coli, 71.29 % for S. aureus and 60.18 % for MDR K. pneumoniae. Post-treatment observations revealed notable differences in biofilm formation across bacterial isolates. Furthermore, AgNP treatment led to significant downregulation of expression of the efflux pump genes acrB, acrE, acrF, and emrB in Gram-negative isolates and norB in Staphylococci isolates. This research underscores the potential for the development of an eco-friendly antimicrobial alternative in the form of green synthesized silver nanoparticles to combat drug resistance offering potential antibiofilm and efflux pump inhibitory activities.

Integration of Biochar with Chemical Fertilizers Improves the Economic Yield, Quality of Holy Basil (Ocimum sanctum L.) and Soil Health

Integration of Biochar with Chemical Fertilizers Improves the Economic Yield, Quality of Holy Basil (Ocimum sanctum L.) and Soil Health

Evaluation of antidiabetic activity of Ocimum sanctum (Tulsi) through inhibition of alpha-amylase and alpha-glucosidase: an in vitro study

Evaluation of antidiabetic activity of Ocimum sanctum (Tulsi) through inhibition of alpha-amylase and alpha-glucosidase: an in vitro study

Evaluation of Antibacterial Effects of an Oral Rinse Containing Ocimum tenuiflorum and Ocimum gratissimum on Streptococcus mutans and Lactobacillus Species.

Dental caries, primarily caused by Streptococcus mutans and Lactobacillus spp., is a major global health issue. There is a growing need for effective, natural antimicrobial treatments. Ocimum tenuiflorum and Ocimum gratissimum are known for their medicinal properties, including antimicrobial activity. This study investigates the antibacterial efficacy of a herbal oral rinse derived from these plants. This study aimed to evaluate the antibacterial activity of an herbal formulation-based oral rinse prepared from Ocimum tenuiflorum and Ocimum gratissimum against Streptococcus mutans and Lactobacillus spp. Fresh leaves of Ocimum tenuiflorum and Ocimum gratissimum were shade-dried, powdered, and extracted in distilled water. The extract was incorporated into an oral rinse formulation. The antibacterial activity was assessed using the agar well diffusion method, protein leakage and cytoplasmic leakage assays, and time-kill curve analysis. A commercial oral rinse was used as a standard. The herbal oral rinse exhibited significant antibacterial activity against both Streptococcus mutans and Lactobacillus spp. The zones of inhibition for Streptococcus mutans were 10 mm, 13 mm, and 15 mm at concentrations of 25 µg/mL, 50 µg/mL, and 100 µg/mL, respectively. For Lactobacillus spp., the inhibition zones were 10 mm, 12 mm, and 14 mm at the same concentrations. The protein leakage and cytoplasmic leakage analysis supported these findings, demonstrating the formulation's efficacy at low concentrations. Time-kill curve assays showed rapid bactericidal action, particularly at higher concentrations. The Ocimum tenuiflorum and Ocimum gratissimum-based herbal oral rinse demonstrates strong antibacterial activity against key oral pathogens, suggesting that it could be a natural alternative to conventional oral rinses.

Recent developments in the therapeutics of SARS-CoV-2 infection

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmittable respiratory virus that causes COVID-19 disease. Since its emergence in the early 2000s, SARS has posed significant public health challenges and continues to be a concern. This review provides an overview of current treatment strategies for SARS-CoV and explores the potential utility of herbal molecules in combating this viral infection. The primary treatment approach for SARS-CoV has historically centered on supportive care, which includes oxygen therapy, mechanical ventilation, and antipyretic medications to manage symptoms. However, with the ongoing advancement of medical research and our evolving understanding of the virus, several antiviral drugs have been developed and repurposed to target SARS-CoV. Recent developments in the field of herbal medicine have drawn attention to the potential efficacy of natural compounds in the management of viral infections, including SARS. Herbal molecules, characterized by their diverse bioactive constituents, exhibit antiviral properties that could be harnessed to mitigate SARS symptoms and inhibit viral replication. Ocimum sanctum, Tinospora cordifolia, Zingiber officinale, Curcuma longa, Panax ginseng, and Aloe vera have been identified for their immunomodulatory effects. This review highlights promising herbal molecules such as quercetin, and curcumin, which have demonstrated antiviral effects in–vitro and in some preclinical studies. While current treatment strategies for SARS primarily rely on supportive care and antiviral medications, the exploration of herbal molecules presents an exciting avenue for potential adjunctive therapies. Further research is required to validate their efficacy, safety profiles, and mechanisms of action. A holistic approach that combines conventional medicine with herbal remedies may offer new insights into the treatment of SARS and other viral respiratory illnesses.

Green synthesis of ZnO nanoparticles and biological applications as broad-spectrum bactericidal, antibiofilm effects and biocompatibility studies on Zebrafish embryo

This work generated zinc oxide nanoparticles (ZnO NPs) using Ocimum tenuiflorum in an inexpensive and environmentally friendly manner. The generated ZnO NPs were analysed using Ultra visible spectroscopy (UV–Vis), Fourier transformed infrared spectroscopy (FTIR), Scanning electron microscopy-Energy X-ray diffraction spectroscopy (SEM-EDX), Transmission electron microscopy (TEM), and X-ray diffraction (XRD). Bio-synthesized ZnO NPs showed UV–Visible absorbance maxima at 290 nm, indicating bio reduction and stability. The DRS spectra revealed a straight band gap value of 3.24 eV. Alcohols, alkanes, phenols, and amines in FTIR may contain reduced and capped zinc ions. Crystalline ZnO nanoparticles were verified by XRD. Biosynthesized ZnO NPs were exhibited in spherical and 5–35.49 nm in size by FESEM and TEM. EDX confirmed NPs zinc elemental signatures. Antibacterial properties showed that ZnO NPs effectively inhibited gram (+ve) and gram (−ve) pathogens, including E. coli (20.0 ± 0.8 mm), E. faecalis (20.0 ± 0.5 mm), S. aureus (18.01 ± 1.2 mm), and S. mutans (17.5 ± 1.0 mm). This study shows that biosynthesized ZnO NPs inhibit biofilm growth by 0.198–1.431 O.D values (Optical density). Antibiofilm inhibition was concentration-dependent. Nanoparticles proved effective against E. coli, S. mutans, S. aureus, and E. faecalis with MICs of 15 and 25 µg/mL. Time-dependent death kinetic studies indicated that 8–12 h ZnO NPs killed all bacteria. Also, ZnO NPs had low harmful effects on zebrafish eggs at doses upto 1 mg/mL. In summary, the production of green ZnO NPs offers a sustainable and biocompatible approach that is well-suited for various biological applications.

Evaluation of antifungal activities of some Indian dietary spices against pathogenic Rhizopus arrhizus and Rhizopus stolonifer

Rhizopus arrhizus and Rhizopus stolonifer are pathogenic fungi responsible for causing ‘soft rot’ disease in fruits and vegetables, as well as life-threatening ‘mucormycosis’ in animals and humans, especially immunocompromised hosts. The use of synthetic fungicides and chemical preservatives to inhibit microbes is being challenged due to their toxic effects on human health and the environment. This has prompted researchers to investigate novel antimicrobials that are natural and safe. Current study aimed to evaluate the antifungal activities of essential oils (EOs), powdered (PWD) forms, and water extracts (WEs) of ten Indian dietary spices (Allium sativum (Als), Brassica nigra (Brn), Cinnamomum zeylanicum (Ciz), Cuminum cyminum (Cuc), Curcuma longa (Cul), Mentha piperita (Mep), Ocimum sanctum (Ocs), Syzygium aromaticum (Sya), Trigonella foenum-graecum (Trf), and Zingiber officinale (Zio)) against R. arrhizus (NCIM877) and R. stolonifer (NCIM1139). Spice agar method was used for investigating antifungal potential of PWD spices. Impregnated paper disc method and broth dilution technique were followed for screening the antifungal activities of EOs and WEs. Results revealed that EOs of six spices (Als, Brn, Ciz, Cuc, Mep, Sya), and PWD forms of four spices (Brn, Ciz, Cuc, Sya) inhibited the growth of both fungi in culture media, effectively. WEs of all ten spices remained ineffective in arresting the growth of tested fungal strains. R. stolonifer was found more sensitive compared to R. arrhizus towards tested substances. Based on these findings, further in vivo studies on PWD forms of Ccb, Ccm, and Sya and EOs of Als, Brn, Ccb, Ccm, Mep, Ocs and Sya may establish spices as ‘botanical antimicrobials’ to prolong the shelf life of valuable horticultural crops by arresting the growth of R. arrhizus and R. stolonifer.

Biogenic synthesis and characterization of zinc oxide nanoparticles for effective and rapid catalytic reduction of 4-nitrophenol

This paper discusses the biogenic synthesis of zinc oxide (ZnO) nanoparticles by using aqueous solutions of zinc acetate dihydrate and Ocimum tenuiflorum leaf extract. Field emission scanning electron microscopy (FE-SEM) confirms the formation of fine ZnO aggregates. Crystallographic structure and functional group of the nanoparticles are determined by X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) techniques. UV–visible and fluorescence spectroscopies are employed to assess optical behaviour of ZnO nanoparticles.The ZnO nanoparticles show nearly 100 % catalytic efficiency in the sodium borohydride (NaBH4)-mediated reduction of 4-nitrophenol within 60 s. Also, the catalyst can be reused for five cycles without losing 100 % regeneration efficiency. This study demonstrates that the prepared ZnO nanoparticles can effectively detoxify 4-nitrophenol from an aqueous solution.

Exploration of medicinal plants as potential therapeutics against COVID-19: molecular insights and drug development prospects with other significant medicinal information a retrospective exposition.

The study aims to explore the potential of medicinal plants and their phytoconstituents as effective inhibitors of the coronavirus disease 2019 (COVID-19), caused by the SARS-CoV-2 virus. The focus is on investigating specific medicinal plants known for their pharmacological properties, such as: antioxidant, anti-inflammatory, and immunomodulatory effects, to determine their viability in developing COVID-19 treatments. This study involves a comprehensive study of medicinal plants, including: Withania somnifera (Ashwagandha) and Ocimum sanctum (Holy Basil), known for their beneficial health effects. Molecular docking studies were conducted to assess the interactions between phytoconstituents from these plants and SARS-CoV-2 proteins. The compounds' drug-like characteristics and safety profiles were also evaluated to determine their potential as therapeutic agents. The molecular docking studies revealed that the phytoconstituents from the studied medicinal plants exhibit favourable interactions with SARS-CoV-2 proteins, suggesting their potential as therapeutic targets. These compounds demonstrated promising drug-like characteristics and safety profiles, indicating their suitability for further development as COVID-19-fighting medications. The results indicate that medicinal plants and their bioactive substances hold significant potential for developing therapies against COVID-19. The ability of these organic substances to interact with key viral proteins and provide various therapeutic benefits highlights their potential as multi-functional treatment options. However, further research is necessary to confirm these findings and to understand the full scope of their therapeutic efficacy and safety in clinical settings. Medicinal plants and their phyto-constituents represent a promising avenue for developing effective treatments for COVID-19. The favourable interactions with SARS-CoV-2 proteins and the promising drug-like characteristics observed in this study suggest that these natural compounds could be integral in the fight against the COVID-19 pandemic. Further research and clinical trials are essential to fully validating their potential and translating these findings into practical medical applications.

Changes in composition, yield, antimicrobial and antioxidant activities of the Ocimum tenuiflorum L. essential oils as affected by fertilizers

The purpose of this study is to evaluate the impact of multifunctional microbial fertilizer and inorganic macronutrient fertilizers N, P, K on plant biomass, essential oil yield, chemical composition, antimicrobial and antioxidant activities of tulsi (Ocimum tenuiflorum L.) grown in Hanoi, Vietnam. Among the four formulas tested, F2 emerged as the most favourable, comprising a basal fertilization of 10 tons of decomposed manure, 15 kg of multifunctional microbial fertilizer, 150 kg of P fertilizer, and 75 kg of K fertilizer, together with top dressing of 200 kg of N fertilizer and 75 kg of K fertilizer per hectare. This formula yielded the highest tulsi dry biomass (4.73 ton/ha), essential oil yield (0.831%), and essential oil production (39.29 L/ha), which were significantly different (p < 0.001) from those of the remaining formulas. In addition, the concentration of the essential oil main compound - eugenol (63.1%) of tulsi in F2 was also the highest, which may be the cause of the strongest antibacterial activity against Bacillus subtilis, Escherichia coli (IC50 of 320, 273 µg/mL, respectively), and antifungal activity against Candida albicans (IC50 of 400 µg/mL), as well as antioxidant activity (IC50 of 0.165 µg/mL) compared to the other formulas tested. The results suggested the role of both multifunctional microbial fertilizer and inorganic macronutrient fertilizers N, P, K on growth and essential oil biosynthesis in tulsi. These findings are important in identifying the most efficient fertilization formula for cultivating tulsi, serving the needs of the cosmetic and medicinal industries.

DIETARY EFFECTS OF TULSI LEAVES AND BLACK PEPPER EXTRACT ON BROILER GROWTH PERFORMANCE AND CARCASS TRAITS

This study was conducted to determine the efficacy of the dietary effect of different levels of Tulsi leaves (Ocimum sanctum) and Black Pepper (Piper nigrum) Extract (TBE) on growth performance and carcass traits of commercial broiler. The study was conducted at the Poultry farm of Hajee Mohammad Danesh Science and Technology University, Dinajpur during the period from 11 April to 08 May, 2019. A total of 96 day old broiler chicks (Lohman Meat) were randomly and equally distributed to 4 groups having 3 replicates of 8 birds each. The birds were grouped as T0, T1, T2 and T3 group with normal water (control group), 1% TBE 1ml /L of drinking water, 2% TBE 2 ml/L of drinking water and 3% TBE 3ml /L of drinking water, respectively. Body weight gains, Feed Conversion Ratio (FCR), mortality (%) and meat yield traits were recorded. The collected data were analyzed by using ANOVA with SPSS version 22. Feed intake (g/bird) was almost similar (P>0.05) among the dietary treatment groups. Body weight gain (g) and FCR were significantly (P<0.05) differed among the dietary treatment groups. The highest body weight gain (g) was significantly (P<0.05) higher in T2 (1659.35g±2.19), followed by T1 (1631.05g±2.52), T3 (1590.67g±2.73) and T0 (1472.01g±4.58), respectively. The lowest FCR was found in T2 (1.41±0.00) and the highest FCR in T0 (1.52±0.01), the intermediate in T1 (1.45±0.01) and T3 (1.46±0.01), respectively. It was found that there was significant (P<0.05) difference among the dietary treatment groups in case of live weight (g), carcass weight (g),dressing percentage (%), breast weight (g), liver weight(g), but there was almost similar (P>0.05) among the dietary treatment groups in case of heart weight (g), spleen weight (g), gizzard weight (g), thigh weight (%), head weight (g), intestine weight (g) and shank weight (g). The carcass meat weight, Carcass yield (%), breast weight and breast meat weight (%) was significantly (P<0.05) higher in treatment T2 group compared to control group T0. Liver weight was significantly (P<0.05) higher in control group T0 compared to treatment group T2. No mortality was found among the dietary treatment groups during experimental period. Based on the result it could be concluded that 2% Tulsi leaves and Black Pepper Extract (TBE) 2 ml/L of drinking water may be used in broiler diet as growth promoter and it may also serve as an antioxidant.

Effectiveness of natural herbs on sewage and treated municipal wastewater: A comparative study

Improperly handled sewage is the main cause of water pollution. Nearly 62% of wastewater in urban India remains untreated or partially treated, which further gets disposed of in the natural water bodies. The usual treatment methods include the activated sludge process, oxidation ponds, aerated lagoons, and trickling filters. These methods need spacious areas, a lot of energy and time, which drives up the cost of the treatment. The expense of the treatment process can be decreased by using natural coagulants. The indigenous medical system uses plants, which are abundant in secondary metabolites, to treat a variety of illnesses. The purpose of this study was to comprehend how these indigenous plants can be utilized for treating sewage water, as a low-cost accessible method that can be used by citizens to get access to clean drinking water. Material & Methods: A quick and low-cost method was created to investigate the efficacy of Moringa oleifera (Moringa), Ocimum sanctum (Tulsi), and Azadirachta indica (Neem) in the treatment of sewage water. This herb treated water was then compared to municipal treated water from the Ghatkopar Wastewater treatment facility, managed by the Municipal Corporation of Greater Mumbai (MCGM), Mumbai, India. All the herb-based components were bought from regional markets, and water samples from a lagoon found in the nearby Ghatkopar lagoon in Mumbai. Results: When compared to the lagoon-treated water, the efficacy of the sewage water treated with herbs was higher or comparable. Coliform and copper levels decreased considerably in the treated sample. Herb-treated samples showed a reduction in the Chemical Oxygen Demand (COD) levels as compared to the treated water. Conclusion: When combined, the herbs had a significant impact on lowering the COD, Copper, and coliform levels, making the native plants a valuable source for the treatment of sewage water.

Green fuel mediated Europium doped Lanthanum ferrites Synthesis, characterisation of and their application as photocatalyst and antibacterial agents

The focus of multiapplication of the nanomaterial is the recent research. Based on that the current work describes synthesis of nano Lanthanum ferrites doped with Europium (1 mol, 3 mol, 5 mol and 7 mol%) by cost effective biofuel combustion method, using Ocimum tenuiflorum extract as fuel. The doping possibly changed the size and morphology of the host as evident from results of XRD, SEM and TEM analysis. As there is increase in band gap from 2.2 to 2.5 eV for undoped to 5 mol% doped Europium which strongly supports the photocatalytic degradation of Congo Red dye in the UV and visible light. The 5 mol% doped Eu shows 65 % and 81 % in UV-light and Visible light respectively. The electrochemical studies indicates the accepted capacitance and semiconductor nature of synthesised nano ferrites. The antibacterial activity was tested against Escherichia coli and Staphylococcus aureus showed 5 mol% Eu doped LFO was more effective than other synthesized LFO.