Occurrence Of Parasites Research Articles

Giardia nosode 30cH in the treatment of howler monkey (Alouatta guariba) with giardiasis in captivity in Brazil

The red howler monkey (Alouatta guariba) is an endemic primate of the Atlantic Forest, classified as vulnerable. They're susceptible to giardiasis, impacting human health and Alouatta genus conservation. Studies report parasite occurrence in wild and captive individuals. While asymptomatic infections are common in captivity, immunosuppression can lead to severe cases. São Paulo's Wildlife Division maintains a large captive population. To assess Giardia duodenalis presence, fecal samples were collected from 43 individuals in 17 enclosures over three days, totaling 153 examinations. Animals were asymptomatic, with some groups showing loose stools. Two processing methods (direct and Sheather) were used, followed by quantitative evaluation based on cysts per slide. Only one enclosure tested negative; others were rare (4), slight (8), or moderate (4). Despite being asymptomatic, treatment was chosen to prevent positive animals from spreading infection. Allopathic treatment was impractical due to enclosure size; homeopathic Giardia 30cH nosode was given via water and food for 15 days to 16 positive enclosures. Afterward, 144 fecal samples were reanalyzed over three days. Seven enclosures were negative, 8 were rare, one was slight, none were moderate. All had firm feces. Kruskal-Wallis test confirmed significant treatment (p=0.004). Giardia 30cH nosode reduced parasitism, environmental contamination, with easy administration and no stress/adverse effects.

Diversity, prevalence and risk factors associated to gastrointestinal tract parasites in wild and domestic animals from Pakistan.

The gastrointestinal tract (GIT) of animals contains various helminth parasites, responsible for subclinical and clinical parasitism. The health situation of animals is seriously affected by these parasites and leads massive financial losses for the livestock trade. The present study was conducted to determine the prevalence and associated risk factors of GIT parasites in small and large ruminants of Mardan district and zoo animals of Golden Life Safari Park Khyber Pakhtunkhwa. A total of 419 fecal samples were collected including 117 from goats, 110 from cattle, 80 from sheep, 66 from buffaloes, 40 from birds, four from deer, and two from leopards. The fecal samples were subjected to sedimentation and floatation to identify parasite eggs. Of a total of 419 fecal samples, 215 (51.31%) were found positive for GIT parasites eggs. The prevalence of GIT parasites was high in sheep (65%), followed by goats (62.39%), cattle (42.70%), and buffaloes (37.87%). In birds, GIT parasite eggs were identified in 11 (45.83%) and 7 (43.75%) fecal samples of Pheasant and Peacock, respectively. However, an absence of infection was noted in fecal samples from deer and leopards. Eggs of GIT Parasites belonging to one superfamily and four different genera were recognized in fecal samples of domestic animals and buffaloes, which were Trichostrongyloidea (19.81%), Strongyloides (9.31%), Trichuris (7.77%), Monezia (6.44%), and Fasciola (4.53%). For bird, the coccidia group comprises the highest representation of parasites (2.63%), followed by the Trichuris genus (1.67%). Settlement, management system, anthelminthic medications and body condition score were significantly associated with the occurrence of GIT parasites in feces (P < 0.05). Animals from rural settlements, rearing under poor management systems, with thin (poor) body condition and under warmed were more affected by GIT parasites. The diversity and the high prevalence of GIT parasites reported in this study highlights the urgent need for intervention through treatment and modification of management practices in the investigated areas of Pakistan.

Prevalence and risk factors associated with gastrointestinal parasites in goats (Capra hircus) and sheep (Ovis aries) from three provinces of China

Gastrointestinal (GI) parasites in small ruminants, especially goats and sheep, have caused significant socio-economic and public health challenges worldwide. The aim of the present study was to investigate the diversity and prevalence of GI parasites in goats and sheep in Jiangsu, Shaanxi and Hunan provinces of China, and to assess whether the age of animals, sampling season and feeding mode influence the distribution and infection of GI parasites. A total of 1,081 fecal samples collected from goats (n = 835) and sheep (n = 246) were detected by saturated saline flotation technique and nylon sifter elutriation and sieving method for eggs/oocysts, respectively. Based on the morphological observation of eggs and oocysts, one tapeworm, five nematodes, three trematodes and nineteen coccidia were identified, of which seven helminths belong to zoonotic parasites. The infection rate of parasites was 83.4% (902/1081) in total samples, 91.6% (765/835) in goats, and 55.7% (137/246) in sheep. The infection rate of coccidia was 71.0% (767/1081), and that of helminths was 56.2% (607/1081). The dominant species was E. alijeri (67.3%, 562/835) in goats, E. parva (30.1%, 74/246) in sheep. The highest prevalent helminths were Trichostrongylidae spp. in goats (58.3%, 487/835), and Moniezia spp. in sheep (22.76%, 56/246). Of 902 positive samples, 825 (91.5%, 825/902) contained multiple (2–10) parasites. The feeding mode, sampling season and regions were relevant risk factors which have significant influence on the occurrence of GI parasites in goats and sheep. The risk coefficient of parasite infection in autumn was 2.49 times higher than spring (Odds ratio = 2.49, 95% CI = 1.51–4.09, p &amp;lt; 0.001). Compared to raising on the high beds, the goats and sheep raising on the ground had the higher risk of parasite infection (OR = 3.91, 95% CI = 2.07–7.40, p &amp;lt; 0.001). The risk coefficient of parasite infection in Shaanxi and Hunan was 3.78 and 1.25 times higher than that in Jiangsu (OR = 3.78, 95% CI = 2.01–7.12, p &amp;lt; 0.001; OR = 1.25, 95% CI = 1.21–1.29, p &amp;lt; 0.001). These data are significant for the development of prevention strategies to minimise economic losses from small ruminant production and to reduce the risk of water and food infecting humans as vectors of zoonotic parasitic diseases.

Preliminary study on the occurrence of intestinal parasites in Malayan tapir (Tapirus indicus) in Zoo Negara, Malaysia

A preliminary study was conducted to investigate the occurrence of intestinal parasites in the faeces of captive Malayan tapir in Zoo Negara, Malaysia. A total of 12 fresh faecal samples were collected from three different individuals and screened for eggs and larvae using the sedimentation technique. The results shows that Strongylus sp. infection was the highest with 43 %, followed by Strongyloides sp. and Fasciola sp. with 17 % each, Balantidium sp. 11 %, Ascaris sp. 9 % and unidentified 3 %. Fasciola sp. and Ascaris sp. were found in samples obtained from one individual kept in a cage while Balantidium sp. was found in the free-roaming individuals. Previous information on parasitic diseases in tapir was largely derived from those found in the wild. This study highlights the first record on parasitic infection in captive tapir in Malaysia. In addition, due to pathogenic importance of the parasites to both animals and humans, the population should be closely monitored. Considering the endangered status of the Malayan tapir, further studies are needed to highlight this issue.

Host-microbiota-parasite interactions in two wild sparid fish species, Diplodus annularis and Oblada melanura (Teleostei, Sparidae) over a year: a pilot study

BackgroundThe microbiota in fish external mucus is mainly known for having a role in homeostasis and protection against pathogens, but recent evidence suggests it is also involved in the host-specificity of some ectoparasites. In this study, we investigated the influence of seasonality and environmental factors on both fish external microbiota and monogenean gill ectoparasites abundance and diversity and assessed the level of covariations between monogenean and bacterial communities across seasons. To do so, we assessed skin and gill microbiota of two sparid species, Oblada melanura and Diplodus annularis, over a year and collected their specific monogenean ectoparasites belonging to the Lamellodiscus genus.ResultsOur results revealed that diversity and structure of skin and gill mucus microbiota were strongly affected by seasonality, mainly by the variations of temperature, with specific fish-associated bacterial taxa for each season. The diversity and abundance of parasites were also influenced by seasonality, with the abundance of some Lamellodiscus species significantly correlated to temperature. Numerous positive and negative correlations between the abundance of given bacterial genera and Lamellodiscus species were observed throughout the year, suggesting their differential interaction across seasons.ConclusionsThe present study is one of the first to demonstrate the influence of seasonality and related abiotic factors on fish external microbiota over a year. We further identified potential interactions between gill microbiota and parasite occurrence in wild fish populations, improving current knowledge and understanding of the establishment of host-specificity.

Occurrence and Phylogenetic Analysis of Zoonotic Enteropathogenic Protist Parasites in Asymptomatic Domestic Ruminants from Portugal.

Enteropathogenic parasites are of significant concern for public health due to their zoonotic potential and their impact on human and animal health. In this study, we investigated their occurrence and characterized these enteropathogens in asymptomatic domestic ruminants from Portugal. A total of 302 stool samples were collected from cattle (n = 166), sheep (n = 73), and goats (n = 63) in various regions of Portugal and tested for Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi, Blastocystis sp., and Balantioides coli by PCR. The occurrence of Cryptosporidium spp. was found to be 12.7% (8/63, 95% confidence interval [CI]: 5.65-23.5) in goats; however, no sample was found to be positive for Cryptosporidium spp. in cattle and sheep. For E. bieneusi, 6.35% (4/63; 95%CI: 1.76-15.47) of goats were found to be positive; however, no cattle or sheep were found to be positive. Blastocystis sp. was found in sheep (9.59%; 7/73; 95% [CI]: 0.394-18.76) and goats (12.70%; 8/63; 95% [CI]: 5.65-23.50) but none was found in cattle. No positive results for G. duodenalis or B. coli were detected in this study. This study provides essential baseline information for understanding the silent shedding and epidemiology of these enteropathogens in Portugal, contributing to overall livestock health and related occupational safety. Raising awareness among consumers, veterinarians, and farm owners is crucial to minimize the risk of transmission and promote effective disease control strategies.

Integrating databases for spatial analysis of parasite-host associations and the novel Brazilian dataset

Incomplete information on parasites, their associated hosts, and their precise geographical location hampers the ability to predict disease emergence in Brazil, a continental-sized country characterised by significant regional disparities. Here, we demonstrate how the NCBI Nucleotide and GBIF databases can be used as complementary databases to study spatially georeferenced parasite-host associations. We also provide a comprehensive dataset of parasites associated with mammal species that occur in Brazil, the Brazilian Mammal Parasite Occurrence Data (BMPO). This dataset integrates wild mammal species’ morphological and life-history traits, zoonotic parasite status, and zoonotic microparasite transmission modes. Through meta-networks, comprising interconnected host species linked by shared zoonotic microparasites, we elucidate patterns of zoonotic microparasite dissemination. This approach contributes to wild animal and zoonoses surveillance, identifying and targeting host species accountable for disproportionate levels of parasite sharing within distinct biomes. Moreover, our novel dataset contributes to the refinement of models concerning disease emergence and parasite distribution among host species.

Distribution of the gill parasite Discocotyle sagittata (Monogenea: Polyopisthocotylea) in parr of Atlantic salmon Salmo salar and sea trout S. trutta in Norwegian rivers

The monogenean gill parasite Discocotyle sagittata is reported from parr of Atlantic salmon Salmo salar and sea trout S. trutta in the anadromous part of 86 out of 223 Norwegian rivers. The prevalence in each river varied from 1.5% to 88.9%. This study has significantly increased our knowledge about the occurrence of this salmonid parasite in Norway. Most likely, D. sagittata has a much wider distribution all over the country and probably also occur in numerous inland lakes, rivers and streams.

PREVALENCE OF INTESTINAL PARASITES AND ASSOCIATED RISK FACTORS AMONG UNDER-FIVE CHILDREN ATTENDING KIBOGORA LEVEL TWO TEACHING HOSPITAL, RWANDA

This study aimed to assess the prevalence of intestinal parasites and associated risk factors among under-five children attending Kibogora Level Two Teaching Hospital in Rwanda. Specific objectives were: to determine the prevalence of intestinal parasites among under-five years children attending Kibogora Level Two Teaching Hospital, Rwanda, to identify the source of water and their utilization in intestinal parasitic occurrence among under-five years children attending Kibogora Level Two Teaching Hospital, Rwanda; and to identify the risk factors associated with intestinal parasites occurrence among under-five children attending Kibogora Level Two Teaching Hospital, Rwanda. Methods: retrospective cross-sectional study design with quantitative approaches at Nyamasheke in June 2022. The study includes a target population of 772 children and a sample size of 263. A questionnaire was administered to collect data on hygiene, sanitation, socio-demographic and economic characteristics (risk factors), and secondary data from 2019-2022 were used. Results: the prevalence of intestinal parasites was 102(38.7%). In this study the prevalence of Ascaris 56(21.3%) was the highest followed by amoeba (Entamoeba histolytica) 24(9.1%), Giardia lamblia 8(3.0%), Trichomonas intestinalis 7(2.6%) and Ankilostoma duodenale 7(2.6%). Other intestinal parasites detected such as Necator americanus and Trichiuris trichiura were identified at less than one percent prevalence. A chi-squared test was used to establish a relationship between different variables. The chi-squared shows that there is no statistically significant association between the marital status category of the children and having latrines at home with a chi-squared value of 3.293 and a p-value 0.183 of there was no statistically significant association. utensils drying site with a chi-squared value of 5.422 and p-value of .000 there was a statistically significant association. Drinking boiled water with a chi-squared value of 7.857a and p-value of .97. Washing hands before a meal with a chi-squared value of 7.857a and p-value of 98. Washing hands after defecation with a chi-squared value of 3.293 and p-value of .193, there is no statistical significance. Finally, the high prevalence of intestinal parasitic infections in under-five children warrants strict control measures for microbial reduction through the utensils-drying site, improved hygiene and sanitation, and treatment of drinking water should be considered.&lt;p&gt; &lt;/p&gt;&lt;p&gt;&lt;strong&gt; Article visualizations:&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;&lt;img src="/-counters-/soc/0015/a.php" alt="Hit counter" /&gt;&lt;/p&gt;

Shiny Cowbird (Molothrus bonariensis) brood parasitism occurrence and impact increases with decreasing forest cover

ABSTRACT Cowbirds brood parasitism has a detrimental effect on the breeding success of their hosts. The occurrence of parasitism observed may be related to environmental features at landscape or nest-site scales. Such relationships have been assessed for the Brown-headed Cowbird (Molothrus ater) in the large forests of the northern hemisphere. Here, we present a study conducted on the Shiny Cowbird (M. bonariensis) in native forests of the southern hemisphere in Argentina. These forests are characterised as semi-open woodlands, with forest fragments of variable sizes immersed in native grasslands. We aimed to evaluate the effect of Shiny Cowbird brood parasitism on the breeding success of a native passerine, the Masked Gnatcatcher (Polioptila dumicola), and to assess the relationship of its occurrence with environmental features at landscape and nest-site scales. During three breeding seasons (2015–2018) we monitored 207 gnatcatcher nests of which 70 were parasitised. Brood parasitism was the cause of nest failure in 60% of the parasitised nests. In addition, breeding success measured by apparent nest success, egg survival, hatching success and nestling survival were significantly lower for parasitised nests than for non-parasitised nests. Brood parasitism occurrence was negatively associated with forest cover, where nests located in sites with less forest cover experienced a higher occurrence of parasitism than those with greater cover. As these native semi-open forests face a continuous decline, our results add to the evidence of negative impacts of deforestation.

Assessment of the Epidemiology of the Gastrointestinal Tract Nematode Parasites in Sheep in Toke Kutaye, West Shoa Zone, Ethiopia.

Gastrointestinal tract nematodes are considered to be the most significant and underestimated problems that hinder sheep productivity. This study aimed to evaluate the epidemiology of gastrointestinal tract nematode infestation of sheep in the Toke Kutaye District of West Shoa Zone, Ethiopia. A cross-sectional study was conducted to determine the prevalence and risk factors associated with gastrointestinal tract nematode parasite infestation in sheep. Faecal samples were collected from 384 sheep and subjected to coprological examination, including direct smear, flotation techniques, and Baermann techniques for screening and identifying gastrointestinal nematodes. The overall prevalence of gastrointestinal tract nematode parasite infestation in sheep in the study area was 284 (73.96%). From the total positive cases, males and females were 97 (82.91%) and 187 (70.04%), respectively. The predominantly detected gastrointestinal tract nematodes of sheep in the study area were Trichostrongylus 111 (28.91%), Oestartagia 55 (14.32%), Haemonchus 42 (10.94%), Oesophagostomum 48 (12.50%), and Trichuris 23 (5.99%). Mixed nematode eggs were noticed in some of the sheep beside the single type of nematode eggs, with a prevalence of 85 (22.14%). The relationship in the occurrence of parasites between sex, age groups, body conditions, and seasons was statistically significant (P = 0.008, P = 0.014, P = 0.001 and P = 0.003), respectively. The present study is of great importance to add to the existing knowledge of the epidemiology of gastrointestinal tract nematodes of sheep, and the findings are very important to apply the proper control and prevention strategies for gastrointestinal tract nematodes of sheep in the area.

Preliminary study on prevalence of hemoprotozoan parasites harbored by Stomoxys (Diptera: Muscidae) and tabanid flies (Diptera: Tabanidae) in horse farms in Nakhon Si Thammarat province, Southern Thailand.

Stomoxys and tabanid flies are of medical and veterinary importance because they play crucial roles in disease transmission as mechanical vectors of various hemopathogens. However, its role as a hemoprotozoan parasite vector in horse farms has not been studied. Therefore, we investigated the occurrence of hemoprotozoan parasites belonging to the genera Babesia, Theileria, and Trypanosoma in Stomoxys and tabanid flies using conventional polymerase chain reaction (PCR) and DNA sequencing. All samples (Stomoxys and tabanid flies) were collected using an Nzi trap for three consecutive days each month from November 2022 to March 2023. The flies were morphologically identified to the species level and separated according to sex. Individual (for tabanid flies) or pooled samples (consisting of three specimens of Stomoxys flies of the same species and sex collected from the same site) were used for DNA extraction. Conventional PCR was used to screen for hemoprotozoan parasite DNA, followed by Sanger sequencing to identify the species. In total, 189 biting flies were collected, including four species of Stomoxys (Stomoxys bengalensis, Stomoxys calcitrans, Stomoxys indicus, and Stomoxys sitiens) and five species of tabanids (Atylotus cryptotaxis, Chrysops dispar, Tabanus megalops, Tabanus mesogaeus, and Tabanus rubidus). Stomoxys calcitrans was the most prevalent species, accounting for 58.7% (n = 111) of the collected flies. Ten (12.4%) of the 81 samples (individuals and pools) analyzed by PCR were positive for the 18S rRNA gene of the Theileria/Babesia species. Trypanosoma DNA was not detected in any sample. After performing Basic Local Alignment Search Tool searches and a phylogenetic analysis, only six samples (7.4%), including S. calcitrans (n = 2), S. sitiens (n = 2), T. megalops (n = 1), and A. cryptotaxis (n = 1), were found to be infected with Theileria sinensis. Furthermore, apicomplexan parasites, namely, Mattesia spp. and Colpodella spp., were found on S. indicus, the fungus Meira spp. was found on S. calcitrans, and the pathogenic green alga Helicosporidium spp. was found on A. cryptotaxis. This study is the first to report a variety of Stomoxys and tabanid flies collected from horse farms in Thailand, which were found to be infected with Theileria and Colpodella species that affect mammals, suggesting that Stomoxys and tabanid flies can be used to confirm the presence of hemoprotozoan parasites in the study area. Understanding the presence of hemoprotozoa in flies could help design vector control programs and manage various diseases in the study area.

Prevalence and public health relevance of enteric parasites in domestic dogs and cats in the region of Madrid (Spain) with an emphasis on Giardia duodenalis and Cryptosporidium sp.

Pet dogs and cats exert an unquestionable beneficial effect in the well-being of their owners, but can also act as a source of zoonotic infections if improperly cared. We investigated the occurrence, risk factors, genetic variability and zoonotic potential of intestinal parasites in dogs and cats attended in a clinical veterinary setting in Spain. Canine (n = 252) and feline (n = 35) faecal samples were collected during 2017-2019 and analysed by coproparasitological methods. A rapid lateral immunochromatographic test (ICT) was used for detecting Giardia duodenalis and Cryptosporidium sp. Samples positive at microscopy examination and/or ICT were reassessed by molecular methods. Overall, 48.8% (123/252) of dogs and 48.6% (17/35) of cats were infected by enteric parasites. In dogs, G. duodenalis was the most prevalent species (40.9%), followed by Cystoisospora sp. (7.1%), and Toxocara canis (5.2%). In cats, Joyeuxiella sp. and Toxocara cati were the dominant species (20.0% each), followed by G. duodenalis (14.3%), D. caninum (5.7%) and Cystoisospora felis and Toxascaris leonina (2.9% each). Pups and kittens were more likely to harbour intestinal parasites and develop clinical signs. Sequence analyses of dog isolates revealed the presence of assemblages A (n = 1), C (n = 4), D (n = 4) and C+D (n = 1) within G. duodenalis; C. parvum (n = 1) and C. canis (n = 4) within Cryptosporidium and PtEb IX (n = 1) in Enterocytozoon bieneusi. A novel C. canis subtype family, named XXi, is reported. Our results highlight that (i) well-cared dogs carry zoonotic enteric protozoan parasites of public health relevance, (ii) proper hygiene practices and routine veterinary treatment are essential to prevent zoonotic infections, (iii) vulnerable populations should avoid contact with pups/kittens with diarrhoea and (iv) infected dogs might be major contributors to the environmental contamination with soil-transmitted helminths (STHs) eggs.

Host body mass, not sex, affects ectoparasite loads in yellow-necked mouse Apodemus flavicollis

We investigated the presence and potential causes of sex bias in ectoparasite infestations in the yellow-necked mouse Apodemus flavicollis. We compared the natural tick and flea burdens of male and female mice in a temperate beech forest and assessed whether the observed differences were driven by host sex or body mass. We found that males were more heavily infested by ticks compared to female mice. However, this difference was driven by host body mass, and not sex itself. Host body mass positively correlated with flea loads, but there was no evidence of sex bias in flea abundance. In addition, the abundance of both ticks and fleas infesting yellow-necked mice changed over time, both seasonally (month to month) and annually (year to year). Our results underscore the importance of the sexual size dimorphism and the parasite taxon as the primary factors that influence the occurrence of sex-biased parasitism in small mammals.

Prevalence of intestinal parasitic infections in HIV-positive and negative groups in some selected semi-urban areas in Nasarawa state, Nigeria

Objectives: The objective of the study is to determine the occurrence of intestinal parasites among human immunodeficiency virus (HIV)-positive and negative groups. Materials and Methods: A case–control study was conducted in four semi-urban areas in Nasarawa State. The study enrolled 422 participants. Stool samples were collected and examined for the presence of intestinal parasites. Blood sample was collected to investigate for HIV infection. Results: The overall prevalence of intestinal infections among HIV-positive individuals was 37.7% (78/207). For geohelminth infections, HIV-positive individuals were more commonly infected by hookworm at a rate of 9.7% (20/207). For Protozoa infections, HIV-positive groups were identified more with Giardia lamblia, at a rate of 8.2% (17/207). The binary logistic regression showed that factors significantly associated with parasite infections among HIV-positive individuals included being under 43 years of age (odds ratio [OR] = 2.192, confidence interval [CI]: 0.082, 3.736) and subjects with diarrhea (OR = 1.521, CI: 0.112, 2.891) for geohelminth parasite. While in the case of Protozoa infections, parasitic infections are higher among study subjects with diarrhea (OR = 1.767, CI: 0.111, 3.009). Conclusion: HIV-positive individuals are more susceptible to coinfections with hookworm (geohelminth) and G. lamblia (Protozoa), which are more prevalent among those with poor personal hygiene habits. This indicates a need for an integrated approach to hygiene promotion, surveillance, and treatment.

Parasitological datas of helminthes parasites of Macrodon atricauda (Günther, 1880)

Ichthyoparasites can affect the health of fish, cause negative economic repercussions and are causes of parasitic zoonoses in the population. The aim of this study was to evaluate the helminth fauna of Macrodon atricauda, ​​as well as to evaluate the zoonotic potential of the parasites that were found. The fishes were collected in two periods in 2014 and 2015. The present study demonstrated that the females presented better conditions than the males, and there was no statistically significant difference between the conditions in both sexes. There is a positive relation regarding total parasite prevalence and fish weight and length. The occurrence of helminth parasites of fish can be dangerous to humans because some species represent a zoonotic potential gives evidence to the need for a change in the hygienic and food habits of the population and the need for control and prevention by the public health agencies.

Prevalence of Parasites Infestation in Cultured and Wild African Catfish in Zaria Kaduna State, Nigeria

The study aimed to investigate the occurrence of gastrointestinal (GIT) parasites in wild and cultured catfish (Clarias gariepinus) in Zaria and its Environs, Nigeria. Two hundred and forty (240) African catfish (Clarias gariepinus) of both sexes were sampled from cultured ponds and some water bodies around Zaria (including Galma dam, river Kubanni, River Saye). The fish were transported to the Department of Veterinary Parasitology and Entomology, Ahmadu Bello University Zaria and used for the study. The catfish were dissected longitudinally and examine for parasites (GIT) grossly, and using Simple flotation technique for parasite eggs and oocysts of helminths and protozoans. Modified Ziehl Neelson staining technique was further performed on oocysts suspected to be Cryptosporidium. The result showed an overall occurrence of 32.5% for GIT parasites in Clarias gariepinus in the study area. The GIT parasites identified in both wild and cultured Clarias gariepinus were Procamallanus laevionchus (46.7%), Capillaria species (13.3%), and some unknown helminths (40.0%). There was a highly significant difference (p = 0.001) in the occurrence of Crytosporidium oocysts in wild Clarias gariepinus (86.2%) compared with cultured Clarias gariepinus (13.8%). Based on the sex; there was an observably higher occurrence of GIT parasites in male catfish (17.1%) than in female (15.4%), although not statistically significant (p = 0.645). The finding of this work revealed that gastrointestinal parasites are prevalent in the Clarias species sampled in Zaria and its Environs. It is hereby recommended that effective control measures and good culinary practices should be adopted to decimate the effect of these parasites in fish, especially those raised in culture ponds to improve productivity.

Prevalence of Intestinal Parasites, Risk Factors and Zoonotic Aspects in Dog and Cat Populations from Goiás, Brazil.

Gastrointestinal diseases caused by parasites are frequently diagnosed in the clinical routine of domestic animals, especially dogs and cats. In general, they trigger factors that can affect human health due to zoonoses. Therefore, this study aims to identify the main intestinal parasites obtained from the fecal samples of dogs and cats in the municipality of Jata, Brazil, and their associated risk factors. Between October 2020 and March 2022, fecal samples were collected from 359 dogs and 55 cats through spontaneous defecation and subsequently subjected to coproparasitological analyses using the Willis fluctuation and Hoffman spontaneous sedimentation techniques. The following parasitic species were identified: Ancylostoma spp., Toxocara spp., Trichuris vulpis, Dipylidium caninum; Giardia spp., Entamoeba spp., Cystoisospora spp., and Platynosomum fastosum. The risk factors associated with parasitism include age, average income of owners, access to garbage, sewage, waste, outdated deworming, and contact with animals. The results demonstrate the need to establish public policies and implement preventive and control measures to reduce the occurrence of parasites in animals and the exposure of humans to pathogenic agents.

Infection levels of Gnathostomatidae (Nematoda) larvae in commercial fishes in north-eastern Australian waters and related food safety concerns

The majority of research on the safety of marine edible fish has primarily focused on anisakid nematodes, neglecting the potential risks posed by other parasites, including those belonging to the family Gnathostomatidae. In Australia, there have been few reported cases of human infections with gnathostomatid parasites since 2011. However, due to the absence of a standardized diagnostic test in the country, it is believed that the actual number of infections is higher than reported. This study aimed to assess the occurrence and prevalence of infectious gnathostomatid parasites in selected commercial fish species in Australia. A total of 1947 marine fish from northern Australia, representing 9 families, 16 genera, and 30 species, were examined for gnathostomatid nematode infections. Overall, 12.3 % of the fish were found to be infected with at least one gnathostomatid larva. Among the species examined, the yellow-dabbled flounder (Branchypleura novaezeelandiae) exhibited the highest prevalence (83.3 %; n = 6) and the largest number of gnathostomatid larvae. The identification of the gnathostomatid larvae was confirmed as belonging to the genus Echinocephalus based on both morphological characteristics and sequence data. No significant correlation was observed between the prevalence, mean abundance, and mean intensity of infection with the length or weight of the examined fish species. Notably, several of the infected fish species are considered popular choices in the Australian market. Hence, it is imperative to raise awareness among relevant food safety authorities regarding the occurrence of these parasites. The findings from this study should be taken into consideration for the revision of current seafood safety protocols in the country.

Survey of intestinal parasites in swine farms raised in Western Nepal.

Pigs (Sus scrofa domesticus), an important domestic livestock, are generally affected by helminth and protozoan parasites. Rearing pigs in rural regions in Nepal is a common practice for subsistence farming. A cross-sectional survey was conducted to determine the occurrence of gastrointestinal parasites (GIPs) in pigs raised in Western Nepal. A total of 200 faecal samples from commercial and smallholder farms were examined by wet mounts, flotation, sedimentation and staining techniques. The results revealed that overall 86.5% of samples were found shedding oocysts or eggs of one or more GIPs. Three species of protozoa [Eimeria sp. (26%), Entamoeba coli (25.5%) and Coccidia (29%)] and nine species of helminths parasites (Ascaris suum (32.5%), Trichuris suis (30%), strongyle-type nematode (27.5%), hookworm (26%), Fasciola sp. (17.5%), Physaloptera sp. (17.5%), Strongyloides sp. (17.5%), Metastrongylus sp. (8%) and Oesophagostomum sp. (5.5%)] were identified. Female pigs were found to have higher protozoan infection than males, but such a difference was not noticed with regard to helminth parasites. Strongyles and Oesophagostomum infection were higher in commercial farms compared to smallholder farms, whereas the prevalences of E. coli and other protozoans were higher in smallholder farms. Among the contextual factors evaluated for association, weight and gender of pigs, and annual income and gender of managers/caretakers were significantly (p<0.05) associated with the prevalence of GIPs in pigs. The overall prevalence of certain helminths such as strongyle-type nematode and A. suum was significantly (p<0.05) associated with the weight of pigs after adjusting other contextual factors. This study detected relatively high prevalence of intestinal parasites in domestic pig facilities. Molecular epidemiological studies are essential to verify the exact zoonotic potential of parasites carried by pigs in the region. An effective periodic monitoring of GIPs of pigs needs to be carried out to minimize their further dissemination.