Occurrence Of Disease Research Articles

Comparison of BoLA-DRB3 genetic diversity among Polish Red-and-White (PRW) and Polish Holstein-Friesian (PHF) cattle

Abstract Cattle breeding in Poland, including the maintenance of indigenous breeds, is exposed to the occurrence of many diseases of infectious and non-infectious etiologies. Bovine MHC (BoLA)-DRB3 is a highly polymorphic gene that plays an important role in the immune response to antigens. This pilot study aimed to analyze BoLA-DRB3 genetic diversity among Polish Red-and-White (PRW) and Polish Holstein-Friesian (PHF) cattle. DNA sequencing revealed haplotypes for 48 out of 106 PRW and 10 out of 26 PHF cows. Analysis indicated 42 alleles detected in the PRW breed and 16 in the PHF animals. Interestingly, within the PRW breed three alleles BOLA03100|BoLADRB3* 001:01|554, BOLA03113|BoLA-DRB3*009:02|270 and BOLA03302|BoLA-DRB3*049:01|236 were observed in 24 cows. Overall, the presented study demonstrated the genetic diversity of the BoLA-DRB3 gene in the indigenous Polish Red-and-White cattle breed for the first time. The acquired results broaden the knowledge of BoLA-DRB3 diversity in cattle, whose genetic variants promote resistance or susceptibility to viral or bacterial diseases.

Binding Mode and Molecular Mechanism of the Two-Component Histidine Kinase Bos1 of Botrytis cinerea to Fludioxonil and Iprodione.

Gray mold caused by Botrytis cinerea is among the 10 most serious fungal diseases worldwide. Fludioxonil is widely used to prevent and control gray mold due to its low toxicity and high efficiency; however, resistance caused by long-term use has become increasingly prominent. Therefore, exploring the resistance mechanism of fungicides provides a theoretical basis for delaying the occurrence of diseases and controlling gray mold. In this study, fludioxonil-resistant strains were obtained through indoor drug domestication, and the mutation sites were determined by sequencing. Strains obtained by site-directed mutagenesis were subjected to biological analysis, and the binding modes of fludioxonil and iprodione to Botrytis cinerea Bos1 BcBos1 were predicted by molecular docking. The results showed that F127S, I365S/N, F127S + I365N, and I376M mutations on the Bos1 protein led to a decrease in the binding energy between the drug and BcBos1. The A1259T mutation did not lead to a decrease in the binding energy, which was not the cause of drug resistance. The biological fitness of the fludioxonil- and point mutation-resistant strains decreased, and their growth rate, sporulation rate, and pathogenicity decreased significantly. The glycerol content of the sensitive strains was significantly lower than that of the resistant strains and increased significantly after treatment with 0.1 μg/ml of fludioxonil, whereas that of the resistant strains decreased. The osmotic sensitivity of the resistant strains was significantly lower than that of the sensitive strains. Positive cross-resistance was observed between fludioxonil and iprodione. These results will help to understand the resistance mechanism of fludioxonil in Botrytis cinerea more deeply.

Host density and anthropogenic stress are drivers of variability in dark spot disease in Siderastrea siderea across the Florida Reef Tract

Dark spot disease (DSD) was first reported within Florida's coral reefs in the 1990s but factors affecting its spatial distribution have not been well studied. We used a 14-yr (2005–2019) coral monitoring data set, utilizing 2242 surveys collected along Florida's coral reefs (about 530 linear km) to explore the spatial and temporal patterns of DSD occurrence. We built predictive statistical models to test for correlations between a suite of environmental and human impact factors and the occurrence of DSD in the reef coral, Siderastrea siderea. DSD in S. siderea is a chronic disease which occurred in all 14 yrs of the study. Annual DSD prevalence ranged from 0.45% to 4.4% and the proportion of survey sites that had DSD ranged from 4.8% to 30.9%. During the study period, DSD became more widespread across Florida's coral reefs and affected a higher proportion of S. siderea populations. Spatial variations in DSD correlated with environmental and human factors which together explained 64.4% of the underlying variability. The most influential factors were concentration of silica in the surface waters (a proxy for freshwater input), the total number of coral hosts, and distance to septic areas. DSD occurred in all regions, but the highest cumulative prevalence occurred in the upper Keys on reefs around major urban centers with links to coastal water discharges. Our results support the hypothesis that coastal water quality is a key component of DSD disease dynamics in Florida and provides motivation for addressing land–sea connections to ameliorate disease occurrence in the region.

Biofloc technology in aquaculture: Advantages and disadvantages from social and applicability perspectives – a review

Abstract The world aquaculture industry seeks sustainable production strategies to respond to the needs of the growing world population for valuable food. Biofloc technology (BFT) is accepted as a novel sustainable method that reduces the pollution load on receiving water resources with its zero-water-discharge approach, eliminates extra water treatment costs with its biological cycles, and supports the growth performance of reared animals and thereby reduces feed costs. However, each BFT system has its own set of difficulties and obstacles. This review focuses on the advantages and disadvantages of BFT systems in terms of economy, sociability and applicability. The BFT shows more effective results in the conversion of toxic nitrogenous compounds to nontoxic compounds compared with the technologies based on nitrification processes. In addition, the growth performance and health of the cultured animals are generally better in BFT systems than those in conventional rearing systems (continuous water flow). The risk of common diseases occurrence due to pathogenic microorganisms appears to be lower in BFT systems compared to conventional aquaculture. It is also noteworthy that the immune system, biochemical stress indicators and antioxidant activities of organisms held in BFT systems are positively affected. In BFT systems based on microbial activity, the high water temperature demanded by bacteria is a limiter for the system. Moreover, a higher aeration/energy requirement is the case in BFT to meet the oxygen demand of all organisms including fish, shrimp, heterotrophic bacteria, nematodes, rotifers and others inside. There is also another concern about the acceptability potential of the cultured organism in BFT systems for consumption. Finally, since the high temperature demand of the system will create high energy costs, it should be considered as another restriction that forces the application. Considering the advantages and disadvantages of BFT systems as sustainable aquaculture, this review provides a guide for future studies and full-scale implementations.

IJCM_305A: Forecasting Dengue and Chikungunya incidence using Time Series Analysis Modelling

Background: In recent years, the rise in vector-borne diseases, particularly Dengue and Chikungunya, has posed a significant public health challenge globally. Epidemiological data from the Integrated Disease Surveillance Program (IDSP) is a useful resource for forecasting and early outbreak identification by applying effective models like Sessional Autoregressive Integrated Moving Average (SARIMA) models has proven effective in capturing temporal dependencies in various fields. Objective: To forecast the dengue and chikungunya cases in India using Seasonal Autoregressive Integrated Moving Average models. Methodology: we preprocessing the IDSP dataset to extract relevant temporal patterns and trends. Subsequently, SARIMA models are applied to capture the time dependent dynamics of disease occurrences. The model’s performance is evaluated through rigorous validation processes, including cross-validation techniques, to ensure its reliability and generalizability. Results: This study considered a secondary data set of weekly dengue and chikungunya cases over the period of 2019 to 2023. The time plot shows several spikes in the data, indicating periods where the number of dengue cases increased significantly in the most recent years. Forecast of the future dengue and chikungunya cases was done for the next one year (2024). we observed that the number of dengue and chikungunya cases has fluctuated over time but has generally remained within a certain range. The forecast predicts a significant increase in cases as we move towards the latter half of 2024, with a very sharp spike towards the end of the forecast period. Conclusion: In conclusion, utilizing a secondary dataset spanning from 2019 to 2023, this study employed time series analysis to forecast future Dengue and Chikungunya cases. The forecast predicts a significant increase in dengue cases during 2024 which can lead to potential implications for public health practitioners, policymakers, and researchers involved in infectious disease management.

Constructing a toilet standard system for the toilet revolution in China

Abstract Access to adequate and secure sanitation to prevent and reduce disease occurrence and improve people's health has been a longstanding global concern. Toilet standardization is immensely important for the promotion of sanitation in China. At present, China has more than 200 toilet-related standards, including national, industrial, provincial, association, and enterprise standards. In addition, there are many existing problems with the standard system, such as standards lagging, standards deficiency, and cross-repetition of standards content. This study analyzed the toilet standards utilizing a six-dimensional structure of the standardized system engineering methodology. A framework for the toilet standard system, which consists of six components, is proposed: basic general standard, public toilet standard, household toilet standard, toilet hardware standard, environmental health and safety standard, and toilet accessory standard and standard toilet peripherals. Additionally, the study suggests approaches for improving standardization including cultivating and developing association and enterprise standards, establishing a standardization technical committee, strengthening standard publicizing and implementation, and linkages with international standards.

Surface Modification of Polyetheretherketone (PEEK) Intervertebral Fusion Implant Using Polydopamine Coating for Improved Bioactivity.

The occurrence of bone diseases has been increasing rapidly, in line with the aging population. A representative spinal fusion material, polyetheretherketone (PEEK), is advantageous in this regard as it can work in close proximity to the elastic modulus of cancellous bone. However, if it is used without surface modification, the initial osseointegration will be low due to lack of bioactivity, resulting in limitations in surgical treatment. In this study, we aimed to modify the surface of PEEK cages to a hydrophilic surface by coating with polyethylene glycol (PEG), hyaluronic acid (HA), and polydopamine (PDA), and to analyze whether the coated surface exhibits improved bioactivity and changes in mechanical properties for orthopedic applications. Material properties of coated samples were characterized and compared with various PEEK groups, including PEEK, PEEK-PEG, PEEK-HA, and PEEK-PDA. In an in vitro study, cell proliferation was found to be enhanced on PDA-coated PEEK; it was approximately twice as high compared to the control group. In addition, mechanical properties, including static and torsion, were not affected by the presence of the coating. Thus, the results suggest that PEEK-PDA may have the potential for clinical application in fusion surgery for spinal diseases, as it may improve the rate of osseointegration.

Olive leaf extract green-formulated manganese nanoparticles: Characterization and antioxidant, cytotoxicity and anti-breast carcinoma properties

Olives are rich in many plant compounds; Antioxidants in olives protect against the occurrence of many cardiovascular diseases, blood pressure, diabetes, various types of cancers and tissue damage caused by free radicals. These antioxidant compounds include oluropine, hydroxytyrosol, tyrosol, oleanolic acid, and quercetin. Recent studies have focused on the therapeutic efficacies of manganese nanoparticles mediated by medicinal plants. The study investigated the characteristics of green manganese nanoparticles formulated using olive leaf extract on breast carcinoma cells. The nanoparticles were analyzed using FE-SEM, XRD, FT-IR, and UV–Vis techniques. The formation of manganese nanoparticles was confirmed by observing bands at 219 and 290 nm. The Mn-O vibration can be identified by the peaks observed at 516 and 610 cm−1. The peaks at 72.69, 60, 53.26, 44.97, 40.25, 34.83, and 28.48 corresponding to manganese nanoparticles (444), (440), (510), (420), (400), (222), and (220) diffraction planes, indicate the manganese nanoparticles formation. The MTT assay was employed to evaluate the anti-breast carcinoma effects (on CAMA-1 and MCF-7 cells) as well as the cytotoxicity effects (on HUVEC cell line) of manganese NPs. Results from the DPPH test showed that MnNPs and BHT at doses of 231 and 84 μg/ml were able to eliminate 50 % of radicals. The findings from the MTT experiment indicate that over 3 days, the cancer cells percentage that survived decreased proportionally to the increase in nanoparticle concentration. Furthermore, the data demonstrate that the cancer cells survival rate decreased with the rise in nanoparticle concentration, ultimately reaching a point where no viable cells were observed at a concentration of 1000 μg/ml. The most effective cytotoxicity was observed at a concentration of 1000 μg/ml. The MTT findings indicate that the IC50 values are 173 and 256 µg/ml, representing the concentration of nanoparticles at which half of the CAMA-1 and MCF-7 breast carcinoma cells in the culture medium are eradicated. Analysis of the gene expression levels of PIK3, AKT, and mTOR, linked to the mTOR pathway, in addition to Bax, Bcl2, and Caspase-3, demonstrates that copper nanoparticles efficiently control PC14 cell apoptosis and proliferation by regulating the PI3K-Akt-mTOR signaling pathway. The mTOR pathway could potentially be related to the suppression of the cell cycle and the initiation of apoptosis due to nanoparticles.

Awareness of Night Eating Syndrome Among College Students (18-29 Years) Native to Manipur

Abstract: Night Eating Syndrome (NES) is a stress-related disorder characterized by morning aphagia, evening hyperphagia and insomnia with the presence of nocturnal ingestions and mood variations which worsen in the evening and night. Presence of eating disorders, psychological disorders, sleep issues like trouble getting to sleep or staying asleep also contribute to this disorder . Night eating syndrome may lead to obesity, metabolic syndrome, diabetes and other life style disorders. The aim of this study is to create awareness of NES and its consequences among college students aged 18-29 years who are native to Manipur. The study collected demographic details and anthropometry measurements of the students and also studied their awareness of NES. Their knowledge about the association of regularly eating post dinner and adverse health outcomes was also studied. The following parameters were considered like age, gender, educational status, BMI category, physical level to study and to create awareness of NES among them. The study used descriptive and exploratory survey design with a quantitative approach to collect data from college students (18-29 years) who are native to Manipur. The sample size was 78 (as per inclusion criteria) consisting of both males and females together. This study revealed that the college students with a percentage of 61.5% from among the total respondents of 52, were not aware of NES and it’s consequences. The limitations of the study suggest further research in the area of Night Eating Syndrome to increase the awareness amongst the population and prevent the occurrence of lifestyle diseases.

Automated Severity Classification Model for Diabetic Retinopathy

Abstract: Diabetic retinopathy is a serious eye disease which is caused due to high blood sugar and it eventually leads to complete blindness. Light sensing tissues of retina will be affected due to this disease. The blood vessels in the eyes will be blocked which leads to leakage of fluid in eye. This condition occurs to people having diabetics for more than a period of twenty years. This disease shows no symptoms at early stage. Later on, cloudy vision or mild blindnessmay occur. The possible cure to this disease is using detection at early stage. If it is detected earlier, we can prevent from blindness. The treatments and technologies present in today's world is time consuming. This system cannot find disease at early stage. Here we are proposing an artificial system reliant on which can anticipate the possibility of occurrence of disease using retina images. The system suggested will classify the stages of disease into five categories and produce an output accordingly. This can help in detecting diabetic retinopathy at early stage and possibly avoid the risk factor of the disease.

Spatial–temporal trends in leprosy burden and its associations with socioeconomic and physical geographic factors: results from the Global Burden of Disease Study 2019

ObjectivesThe purpose of our study was to assess the multiscalar changes in leprosy burden and its associated risk factors over the last three decades. Study designWe conducted an in-depth examination of leprosy's spatial–temporal trends at multiple geographical scale (global, regional, and national), utilizing information from Global Burden of Disease, Injuries, and Risk Factors Study (GBD 2019). MethodsIncidence and the estimated annual percentage change (EAPC) in age-standardized incidence rate (ASIR) of leprosy were determined, with countries categorized based on leprosy incidence changes. We examined socioeconomic and physical geography influences on leprosy incidence via Spearman correlation analysis, using ternary phase diagrams to reveal the synergetic effects on leprosy occurrence. ResultsGlobally, incident cases of leprosy decreased by 27.86% from 1990 to 2019, with a reduction in ASIR (EAPC = −2.53), yet trends were not homogeneous across regions. ASIR and EAPC correlated positively with sociodemographic index (SDI), and an ASIR growth appeared in high SDI region (EAPC = 3.07). Leprosy burden was chiefly distributed in Tropical Latin America, Oceania, Central Sub-Saharan Africa, and South Asia. Negative correlations were detected between the incidence of leprosy and factors of SDI, GDP per capita, urban population to total population, and precipitation, whereas the number of refugee population, temperature, and elevation showed opposite positive results. ConclusionsDespite a global decline in leprosy over the past three decades, the disparities of disease occurrence at regional and national scales still persisted. Socioeconomic and physical geographic factors posed an obvious influence on the transmission risk of leprosy. The persistence and regional fluctuations of leprosy incidence necessitate the ongoing dynamic and multilayered control strategies worldwide in combating this ancient disease.

The influence of job category (rank) in the occurrence of infectious diseases among police personnel in south eastern Nigeria from 2012 to 2021

The occurrence of infectious diseases among police officers is one of the neglected areas in health and scientific research. The aim of this study is to ascertain and assess the influence of job category (rank) in the occurrence of infectious diseases among police personnel in south eastern Nigeria from 2012 to 2021. The study was designed as a retrospective study using data from the central police clinics/hospitals for the period of ten years covering 2012 – 2021. The study population comprised all the recorded infectious disease data in the three police clinics/hospitals of study for police personnel documented from January 2011 to December 2021. Occurrences of infectious diseases were analysed through descriptive techniques such as construction of the frequency distribution and distribution charts. Chi-square test was performed to test for associating variables of interest in the study. The probability value (P) was used to interpret the significance level at 5% and P value less than 0.05 was considered statistically significant. The study found the overall rate of occurrence infectious diseases among police officers as 21.1% in the study area. Significant associating factors of infectious diseases occurrence among police personnel found in the study for job category is (P < 0.0001, χ2=74.91, df = 1). Police officers at junior category were the most affected (24.1%) compared to the officers at senior category (11.9%). the rate of occurrence of infectious diseases is significant among junior category (rank) police personnel in the South Eastern Nigeria.

Palliative care in primary health care: Challenges for training in the Brazilian public system (Unified Health System - SUS)

The improvement in biomedical and healthcare resources has led to an increased life expectancy in the Brazilian population. This aging, coupled with new lifestyle habits, has contributed to the occurrence of non-communicable chronic diseases - particularly cancers, often incurable - making palliative care (PC) emerge as the sole therapeutic recourse. PC involves an approach aimed at promoting the quality of life for patients and their families in the face of therapeutic non-resolvability and the inevitability of life continuation. Early case identification, comprehensive assessment, pain management, and addressing other psychosocial distress are fundamental elements of this practice. Furthermore, healthcare services need to organize themselves in response to this growing demand, with Primary Health Care being a fertile environment for providing this care. Besides allowing geographical, cultural, and emotional proximity of the patient to their loved ones, ensuring their final days with integrity and autonomy. Such care is still offered at other levels, based on the logic of Health Care Networks, comprehensiveness, humanization, referral and counter-referral. The challenge for the curricula of health education courses is to keep up with these changes, seeking to train professionals with a scientific and humanitarian basis consistent with this new clinical reality, whether in specific disciplines – teaching focuses on deepening the technical and human aspects; or through transversal teaching – discussing PC throughout the training, in any discipline. Thus, it is expected that professionals will provide care, offering comfort and tranquility to the patient and their families, collaborating in the humanization of the "ways of dying" in Brazil.

Evaluation of feline heartworm disease based on gross necropsy, serology, pulmonary histopathology, and radiographic evidence in adult shelter cats in northeastern Alabama

Background: Veterinary knowledge regarding feline heartworm has been increasing significantly over the past two decades. Necropsy surveys of shelter cats have shown feline adult heartworm infection prevalence to be 5–20% of the rate in unprotected dogs; however, other studies have shown feline heartworm antibody prevalence up to 33%, reflecting higher exposure rates and potential immature adult infections. Thus, the true prevalence of feline heartworm infection is likely underestimated due to the limitations of current diagnostic techniques, inadequate testing protocols, and the high likelihood of cats exhibiting transient clinical signs or dying without confirmation of infection. Diagnosing Feline Heartworm Disease (FHWD), also referred to as Heartworm Associated Respiratory Disease (HARD), is one of the conundrums of veterinary medicine. The purpose of this study was to evaluate and characterize the occurrence of Heartworm Associated Respiratory Disease [HARD] in shelter cats, naturally-infected with D.immitis.MethodsFifty shelter cats slated for euthanasia between December 2009 and June 2010 were investigated by gross necropsy, radiography, serology, and lung histopathology using techniques that have been established in experimental models of cat heartworm infection. The relationship between pulmonary vascular disease and serological markers for heartworm was also examined using correlations and statistical modeling. Serology included standard heartworm antigen test and a commonly used heartworm antibody test. Also included were heat-treated heartworm antigen test and two additional heartworm antibody tests previously evaluated on experimentally-infected cats.ResultsNone of the cats were heartworm antibody (HW Ab) positive on a commonly used HW Ab test used by many reference laboratories even though 20% of the study cats were heartworm antigen (HW Ag) positive on heat-treated samples. Two additional HW Ab test were positive on 26% and 22% of the study cats. The combination of heat-treated HW Ag, HW Ab tests, and histopathology indicated 34% of the study cats had HARD.ConclusionsUtilizing both, the above tests, and thoracic radiographs, enhanced the ability to predict vascular disease, possibly caused by infection with immature and adult heartworms and supported the premise that cats develop heartworm disease at the same rate as dogs.Graphical

THE USE OF MARE'S MILK POWDER AND SEAWEED POLYSACCHARIDE IN THE DEVELOPMENT OF SPECIALIZED CHOCOLATE FOR HEALTHY DIET

Currently, one of the global problems of world health is the widespread occurrence of chronic non-communicable diseases caused by individual psychological and social correlates of nutrition, as well as insufficient physical activity. One of the ways to solve this problem is to conduct an effective policy of rationalizing the population's nutrition by introducing new specialized food products for dietary and preventive nutrition into the diet. In this regard, it is relevant to develop new healthy food products from the range of confectionery products enriched with various natural biologically active compounds that have pronounced restorative and immunomodulatory properties, using modern biotechnological approaches. The purpose of this research was to study the technological features of the enrichment of chocolate products with sulfated polysaccharide – fucoidan, isolated from brown algae. The article presents data on the use of mare's milk powder and fucoidan in the design of chocolate products with desired properties. In the course of the study, the optimal doses of adding fucoidan powder to the composition of chocolate were studied, and the organoleptic and physicochemical properties of the developed products were evaluated. The applied doses of fucoidan powder were studied at concentrations of 0.001%, 0.005%, 0.01% and 0.05%. The results of the assessment of organoleptic and physico-chemical parameters of laboratory batches of chocolate products showed that the optimal dose of fucoidan powder, as a functional component, is a concentration of 0.01 g per 100 g of the product. Based on the results of the study, the composition and technology for the production of specialized chocolate products was developed.

DAE-CFR: detecting microRNA-disease associations using deep autoencoder and combined feature representation

BackgroundMicroRNA (miRNA) has been shown to play a key role in the occurrence and progression of diseases, making uncovering miRNA-disease associations vital for disease prevention and therapy. However, traditional laboratory methods for detecting these associations are slow, strenuous, expensive, and uncertain. Although numerous advanced algorithms have emerged, it is still a challenge to develop more effective methods to explore underlying miRNA-disease associations.ResultsIn the study, we designed a novel approach on the basis of deep autoencoder and combined feature representation (DAE-CFR) to predict possible miRNA-disease associations. We began by creating integrated similarity matrices of miRNAs and diseases, performing a logistic function transformation, balancing positive and negative samples with k-means clustering, and constructing training samples. Then, deep autoencoder was used to extract low-dimensional feature from two kinds of feature representations for miRNAs and diseases, namely, original association information-based and similarity information-based. Next, we combined the resulting features for each miRNA-disease pair and used a logistic regression (LR) classifier to infer all unknown miRNA-disease interactions. Under five and tenfold cross-validation (CV) frameworks, DAE-CFR not only outperformed six popular algorithms and nine classifiers, but also demonstrated superior performance on an additional dataset. Furthermore, case studies on three diseases (myocardial infarction, hypertension and stroke) confirmed the validity of DAE-CFR in practice.ConclusionsDAE-CFR achieved outstanding performance in predicting miRNA-disease associations and can provide evidence to inform biological experiments and clinical therapy.

Assessment of bidirectional relationships between autoimmune diseases and primary ovarian insufficiency: insights from a bidirectional two-sample Mendelian randomization analysis.

The simultaneous occurrence of primary ovarian insufficiency (POI) and autoimmune diseases has been noted and debated in some epidemiological research. This bidirectional two-sample Mendelian randomization (MR) study aimed to investigate the causal relationships between autoimmune diseases and POI. We obtained summary-level data for ten autoimmune diseases and POI from published large-scale genome-wide association studies and the FinnGen consortium of European ancestry. A series of filtering steps was performed to discern independent genetic variants. Causal estimates were mainly calculated by the inverse variance weighting method and verified through multiple sensitivity analyses. Of the ten autoimmune diseases, genetically predicted Addison's disease (odds ratio [OR] = 1.26, 95% confidence interval [CI]: 1.09-1.47, P = 0.003) and systemic lupus erythematosus (OR = 1.12, 95% CI 1.02-1.24, P = 0.021) were associated with an increased risk of POI, and sensitivity analyses confirmed the robustness of the results. In addition, there were weak associations between liability to POI and elevated risks of type 1 diabetes (OR = 1.05, 95% CI 1.00-1.10, P = 0.046) and autoimmune thyroid disease (OR = 1.03, 95% CI 1.01-1.05, P = 0.015). This study revealed that Addison's disease and systemic lupus erythematosus are potential risk factors for POI, underscoring the necessity to consider the impact of autoimmune factors in the diagnosis and treatment of POI.

Artificial intelligence for disease diagnostics still has a long way to go.

Artificial intelligence (AI) can sometimes resolve difficulties that other advanced technologies and humans cannot. In medical diagnostics, AI has the advantage of processing figure recognition, especially for images with similar characteristics that are difficult to distinguish with the naked eye. However, the mechanisms of this advanced technique should be well-addressed to elucidate clinical issues. In this letter, regarding an original study presented by Takayama et al, we suggest that the authors should effectively illustrate the mechanism and detailed procedure that artificial intelligence techniques processing the acquired images, including the recognition of non-obvious difference between the normal parts and pathological ones, which were impossible to be distinguished by naked eyes, such as the basic constitutional elements of pixels and grayscale, special molecules or even some metal ions which involved into the diseases occurrence.

Production of strawberry cultivars in a closed system of growing on substrate with transplants of different origins

The growing of strawberries in substrate has grown because it is a profitable way of production, easy to work, and which reduces the occurrence of diseases. Like closed growing systems they have a low environmental impact. The imported strawberry transplants bare root are considered more productive, however they arrive late in Brazil, shortening their production cycle, so transplants produced under local conditions would be an alternative to increase producers' income. Thus, the objective of this work was to evaluate the productive result of strawberry transplants produced under local conditions and imported bare root, growing in a closed growing system soilless, on a carbonized rice husk substrate and with the addition of organic compost. Three experimental factors were evaluated: 1st) substrate: carbonized rice husk (CRH) and CRH with 20% organic compound (OC) (CRH + OC); 2nd) cultivars: ‘Camarosa’ and ‘San Andreas’; and 3rd) transplants origin: bare root imported, and runners plants produced under local conditions. The local runner's plants outperformed imported bare root in relation to the number of fruits, however, the imported bare root provided greater average fresh weight of fruits. The runner’s plants of the cultivar 'San Andreas' produced 732.9 g plant-1, higher than the production of imported bare root, with 44.9% of the harvest harvested in the months of July and August. The 'Camarosa' cultivar didn't show the difference in yield between local and imported transplants. Transplants of cv. 'San Andreas' shallow rooted directly into the slabs under local natural conditions can guarantee earlier harvest, greater production, and higher fruit yield. As for the substrate, plant growth and commercial productivity were not influenced by the addition of OC to CRH. The results indicate that the addition of OC to the CRH has no influence on plant growth and commercial fruit production.

The interactions of subcellular organelles in pulmonary fibrosis induced by carbon black nanoparticles: a comprehensive review.

Owing to the widespread use and improper emissions of carbon black nanoparticles (CBNPs), the adverse effects of CBNPs on human health have attracted much attention. In toxicological research, carbon black is frequently utilized as a negative control because of its low toxicity and poor solubility. However, recent studies have indicated that inhalation exposure to CBNPs could be a risk factor for severe and prolonged pulmonary inflammation and fibrosis. At present, the pathogenesis of pulmonary fibrosis induced by CBNPs is still not fully elucidated, but it is known that with small particle size and large surface area, CBNPs are more easily ingested by cells, leading to organelle damage and abnormal interactions between organelles. Damaged organelle and abnormal organelles interactions lead to cell structure and function disorders, which is one of the important factors in the development and occurrence of various diseases, including pulmonary fibrosis. This review offers a comprehensive analysis of organelle structure, function, and interaction mechanisms, while also summarizing the research advancements in organelles and organelle interactions in CBNPs-induced pulmonary fibrosis.