Obvious Effect Research Articles

Digital transformation and corporate green total factor productivity: Based on double/debiased machine learning robustness estimation

With the rapid development of the digital economy, China's digital technology and industry continue to integrate deeply into all areas of society. Digital transformation(DT) has become an important engine and key pedestal for economic and social transformations and upgrades. This paper takes 3525 listed companies from 2013 to 2021 as a sample to measure the green total factor productivity(GTFP) of listed enterprises based on the super-efficiency slacks-based measure with the Global Malmquist-Luenberger Index (SBM-GML) and the super-efficiency epsilon-based measure with the Global Malmquist-Luenberger Index (EBM-GML). It draws on the two existing DT indicators to explore the impact and mechanism of enterprise DT on GTFP. The results show that DT can significantly enhance GTFP, and this conclusion still holds after double/debiased machine learning and other robustness tests; the heterogeneity analysis shows that DT of high-tech enterprises, manufacturing industries and low-financialisation enterprises has a more obvious effect on the enhancement of GTFP; and the four-stage mediated impact mechanism suggests that the effect of DT on GTFP can be achieved by improving internal control ability and technological innovation ability. This paper will provide relevant policy insights on how to better drive enterprise DT and green low-carbon development under the “dual-carbon” goal.

Effects of off-axis angles of 4H-SiC substrates on properties of β-Ga2O3 films grown by low-pressure chemical vapor deposition

In this work, β-Ga2O3 films with (−201) preferred orientation were heteroepitaxially grown on SiC substrates with off-axis angles of 0°, 4° and 8° by low-pressure chemical vapor deposition (LPCVD). It is found that the crystal quality, surface morphology, electrical properties of the β-Ga2O3 films of the film are significantly sensitive to the off-axis angle of SiC substrates. The crystal quality and surface morphology of the film were significantly improved when the 4° off-axis substrate was used, of which the FWHM (Full width at half maximum) was as low as 0.169° and the Ra (Roughness Average) of the smooth surface was 1.98 nm. Due to the improvement of crystal quality, the Hall mobility of the films deposited on 4° off-axis substrates was increased to 50.88 cm2/V·s. The photoluminescence peak intensity of the film increases first and then decreases with the increase of the off-axis angle of the substrate. However, the use of the off-axis substrate has no obvious effect on the oxygen vacancy ratio of the films. The results of this study demonstrate the significance for the preparation of high quality heteroepitaxial β-Ga2O3 films on SiC substrate for the promising applications in power electronic and optoelectronic devices.

An Experimental and Kinetic Modeling Study of the Laminar Burning Velocities of Ammonia/n-Heptane Blends

Ammonia is carbon-free and is a very promising renewable fuel. The ammonia/diesel dual-fuel combustion strategy is an important combustion strategy for ammonia internal combustion engines. To achieve clean and efficient combustion with a high ammonia blending ratio in ammonia engines, it is important to thoroughly investigate the combustion characteristics and chemical reaction mechanisms of ammonia/diesel fuel blends. Based on the constant volume combustion vessel experiments, the laminar burning velocities (LBVs) of ammonia/n-heptane blends were measured at the conditions of an ammonia–energy ratio of 60–100%, at initial pressures of 0.1–0.5 MPa and initial temperatures of 338–408 K, and under an equivalence ratio regime of 0.8–1.3. The experimental results indicate that the laminar burning velocities of ammonia/n-heptane fuel blends increase with a decreasing ammonia–energy ratio. Specifically, with an ammonia–energy ratio of 60%, an initial temperature of 373 K, an initial pressure of 0.1 MPa, and an equivalence ratio of 1.1, the measured LBV is approximately 20 cm/s, which is about 61% faster than that of pure ammonia flames under the same conditions. A previously developed chemical kinetic mechanism is employed to simulate the new experimental data, and the model exhibits overall good performance. The sensitivity analyses have been conducted to highlight the important reaction pathways. The elementary reaction O2 + Ḣ<=>Ö + ȮH demonstrates the most significant promotional effect on the laminar burning velocities, while the interaction reaction pathways of via H-abstraction from n-heptane by ṄH2 radicals are not showing obvious effects on the simulation results under the studied conditions.

A blinded crossover study design to evaluate midazolam as an adjunct for equine standing sedation for routine oral examinations

This study aimed to evaluate the effects of including midazolam to a common equine standing sedation protocol for routine oral examination. Twelve horses underwent two examinations at least seven days apart. Horses were randomly assigned to receive midazolam intravenously (IV) (0.02mg/kg) or a placebo injection of saline (2-2.5mL IV). Five minutes later, detomidine (0.01mg/kg) and butorphanol (0.01mg/kg) were administered IV and horses were placed in standing stocks. A veterinarian blinded to the treatment protocol used a descriptive scoring system to assess degree of ataxia, acceptance of speculum, chewing on the speculum, headshaking, tongue movement, resistance to palpation, and eye appearance as related to the grimace score. During each examination, additional sedation of IV detomidine (0.006mg/kg) and butorphanol (0.006mg/kg) was administered at the discretion of the blinded practitioner to facilitate safe examination. At the second examination horses received the opposite treatment protocol and, following examination, a routine occlusal adjustment. Scores were compared using JMP software with a repeated measures mixed effects model, treatment as a fixed effect and horse and horse/treatment interaction as random effects. Significance was set at P<0.05. There were no significant differences in any of the single or overall sedation scores between treatment groups or within individual horses (P=0.3). Trends towards improvement of some assessed characteristics of sedation, including decreased tongue movement and less resistance to acceptance of speculum were observed. The use of midazolam may prove beneficial for routine oral examination, as well as other standing procedures, with no obvious undesired side effects.

EXPLORING THE EFFECTS OF STITCHING PROCESS ON THE MECHANICAL PROPERTIES OF THREE- DIMENSIONAL (3D) STITCHED UNIDIRECTIONAL COMPOSITES

The stitching process has obvious effects on the out- of- plane mechanical properties of composites such as impact damage mechanism or impact energy absorption. However, the effects on the in- plane mechanical properties of composites have been under discussion and need to be clarified. In the previous studies, tensile, shear and bending behaviors of 3D stitched biaxial woven carbon composite were investigated. The unidirectional carbon woven fabrics constitute the significant part of raw materials in the composite industry. For this reason, the research study was expanded to examine the in- plane mechanics of 3D stitched unidirectional carbon woven composites. In this study, the unstitched and 3D stitched unidirectional woven carbon composites were manufactured and tested under the shear, tensile and bending loads. While the stitching process improved the tensile and shear modulus of composite, it could not create the significant difference in the bending behavior. The highest module and maximum stress values were obtained in the tensile test. The bending and shear test results follow them, respectively. Moreover, it was proven that the fabric architecture of stitched composite layer has the substantial effect on the tensile properties of 3D stitched composite.

Progress in the Treatment of Decubitus Ulcers in Chinese and Western Medicine

Decubitus ulcer is a kind of hard-to-heal ulcer formed due to prolonged bed-ridden illness, localized pressure or long-term friction on the torso, resulting in skin breakage, also known as "Xi-Sore" in Chinese medicine, which is similar to "Pressure Sore" in Western medicine. Decubitus ulcers not only increase the patient's pain and medical expenses, but also increase the treatment and nursing time and nursing difficulty of the patient's family and medical staff, bringing a greater medical and economic burden to society. Decubitus ulcers seriously jeopardize the physical and mental health of patients, significantly reduce the quality of life of patients, and bring great pressure on the survival of their families and a heavy economic burden, as well as causing a great loss of medical resources. Therefore, the search for more effective treatments is a major demand for innovation and development in the field of population and health. Simple Chinese medicine or Western medicine treatment of decubitus ulcers has its limitations, the combination of Chinese and Western medicine treatment of decubitus ulcers has distinctive features and advantages, for decubitus ulcers of all subtypes or periods of time have more obvious therapeutic effect, and effectively prevent the further aggravation of decubitus ulcers, the patient not only reduces the pain and the risk, but also allows the family to reduce the economic burden, it is a worthy of popularization of the treatment method. Therefore, this paper intends to provide a theoretical basis and therapeutic ideas for the systematic diagnosis and treatment of decubitus ulcers by outlining the diagnostic and therapeutic methods of decubitus ulcers.

Lubrication antagonism mechanism of nano-MoS2 and soot particles in ester base oil

Spherical nano-MoS2 (S-MoS2) has excellent lubricating properties and potential application value in engine oil additives. Engine soot can enter the engine oil, so the tribological interaction between S-MoS2 and diesel combustion soot (DCS) should be investigated. In this study, DCS was used to simulate engine soot. The interaction was investigated in dioctyl sebacate (DOS), and the interaction mechanism was full characterized. Results showed that S-MoS2 and DCS had obvious antagonism effects on lubrication. The 0.5% S-MoS2 exhibited good lubricating properties in DOS, which could reduce friction by ∼22% and wear by ∼54%. However, after 0.5% S-MoS2 was added to the 0.5% DCS contaminated DOS, the lubrication performance was not improved and was even worse than that without S-MoS2. When S-MoS2 was added for DOS lubrication, a tribofilm containing MoS2 formed on the friction surface, but simultaneously adding 0.5% DCS resulted in the disappearance of the MoS2 tribofilm. Moreover, under the action of friction heat, DCS and S-MoS2 could form hard MoxCy, thereby increasing abrasive wear. Finally, a preliminary deantagonism method was provided. After 2.0% zinc isooctyl dithiophosphate was added to the above antagonistic system, the friction coefficient did not show visible changes, but the wear recovered to a level close to that when only S-MoS2 was added. The antiantagonism method is not very satisfactory and some more efficient methods need to be further explored.

Antibacterial Activity and Mechanism of Taxillμs chinensis (DC.) Danser and Its Active Ingredients

Taxillμs chinensis (DC.) Danser is a traditional Chinese herbal medicine. It has not been reported regarding antibacterial active ingredients and mechanisms of action. However, the Chinese patent medicine Yinhua Miyanling Tablets containing Taxillμs chinensis has an obvious anti-infective effect in our patent. Therefore, we speculate that Taxillμs chinensis may have antibacterial activity. The purpose of this paper is to study the antibacterial effect and mechanism of Taxillμs chinensis and find active compounds with antibacterial activity and a mechanism. We studied the antibacterial effect and mechanism of Taxillμs chinensis extract. The compounds in the ethyl acetate extract of Taxillμs chinensis were preliminarily identified by UPLC-Q-Orbitrap and analyzed by mass spectrometry. Above all, the antibacterial effect and antibacterial mechanism of the active components of Taxillμs chinensis were determined. Finally, we found, for the first time, that Taxillμs chinensis has a good antibacterial effect and ethyl acetate extract has the best effect. In addition, we found, for the first time, that it has an active component, 4-indolecarbaldehyde, and the component has a good broad-spectrum antibacterial effect. Above all, the active chemical 4-indolecarbaldehyde of Taxillμs chinensis can destroy the bacterial structure, make it unable to maintain normal morphology, and significantly increase the number of deaths. In short, Taxillμs chinensis has an antibacterial effect, and one of its main antibacterial components is 4-indolecarbaldehyde. The antibacterial mechanism of Taxillμs chinensis and 4-indolecarbaldehyde is related to the change in bacterial membrane permeability.

A novel RAV transcription factor from pear interacts with viral RNA-silencing suppressors to inhibit viral infection.

In plants, RNA silencing constitutes a strong defense against viral infection, which viruses counteract with RNA-silencing suppressors (RSSs). Understanding the interactions between viral RSSs and host factors is crucial for elucidating the molecular arms race between viruses and host plants. We report that the helicase motif (Hel) of the replicase encoded by apple stem grooving virus (ASGV)-the main virus affecting pear trees in China-is an RSS that can inhibit both local and systemic RNA silencing, possibly by binding double-stranded (ds) siRNA. The transcription factor related to ABSCISIC ACID INSENSITIVE3/VIVIPAROUS1 from pear (PbRAV1) enters the cytoplasm and binds Hel through its C terminus, thereby attenuating its RSS activity by reducing its binding affinity to 21- and 24-nt ds siRNA, and suppressing ASGV infection. PbRAV1 can also target p24, an RSS encoded by grapevine leafroll-associated virus 2 (GLRaV-2), with similar negative effects on p24's suppressive function and inhibition of GLRaV-2 infection. Moreover, like the positive role of the PbRAV1 homolog from grapevine (VvRAV1) in p24's previously reported RSS activity, ASGV Hel can also hijack VvRAV1 and employ the protein to sequester 21-nt ds siRNA, thereby enhancing its own RSS activity and promoting ASGV infection. Furthermore, PbRAV1 neither interacts with CP, an RSS encoded by grapevine inner necrosis virus, nor has any obvious effect on CP's RSS activity. Our results identify an RSS encoded by ASGV and demonstrate that PbRAV1, representing a novel type of RAV transcription factor, plays a defensive role against viral infection by targeting viral RSSs.

A novel dual-function antiscalant carboxymethyl cellulose-graft-protocatechuic acid for efficient scaling and biofouling prevention

Despite antiscalants playing a critical role in desalination and cooling water systems, their potential to support microbial growth pose a significant threat to water treatment processes by promoting biofouling. In this work, a novel dual-function antiscalant carboxymethyl cellulose-graft-protocatechuic acid (CMC-g-PA) was prepared using plant-derived antibacterial protocatechuic acid (PA) and natural polysaccharide cellulose, and its scale inhibition effect on CaSO4 scale and antibacterial performance on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were evaluated. Results indicated that the inhibition efficiency against CaSO4 scale and the induction time of CaSO4 crystallization process in the presence of CMC-g-PA were remarkably enhanced compared to unmodified CMC. Microscope and XRD characterizations demonstrated significant alterations in the crystal morphology and size of the formed scale using CMC-g-PA as antiscalant. Molecular dynamics (MD) analysis also showed that CMC-g-PA could intensively interact with the crystal plane of CaSO4 with the binding energy reaching 746.73 kcal/mol, thereby affecting crystal growth and causing crystal distortion. Additionally, an obvious inhibitory effect of CMC-g-PA on the growth of E. coli and S. aureus was observed, attributed primarily to the antibacterial activity of the introduced phenolic hydroxyl groups. In short, bio-derived CMC-g-PA, characterized in its environmental-friendliness, antiscaling efficiency and antibacterial activity, holds immense potential for mitigating scaling and biofouling in desalination/cooling water systems.

Effect of the residual levofloxacin on hydroponic vegetables with sewage treatment plant tailwater: Microbial community, discharge risk and control strategy

Tailwater-based hydroponic vegetable is a promising strategy for domestic wastewater recycling. However, the effect of residual antibiotics on the hydroponic vegetable system and the relation between hydroponic culture parameters and the residual water quality are still unclear. Here, the typical antibiotic Levofloxacin (LVFX) was employed, and the effect of LVFX (5 mg/L) on the residual water quality, plant growth and microbial community of water spinach hydroponic culture system were investigated under different hydraulic residence times (HRT). Obvious toxic effects on water spinach were observed, and the highest removal rate of LVFX (about 6 %) and TN (25.67±1.43 %) was observed when HRT was 7 days. Hydroponic culture increased the microbial abundance, diversity, and microbial community stability. To optimize the hydroponic culture, actual sewage plant tailwater spiked with 20 μg/L LVFX, along with three common planting substrates (sponge, ceramsite, and activated carbon) were used for the hydroponic culture of lettuce (seasonal reasons). The inhibition effect of LVFX on the removal of NO3--N and TN was observed even as the LVFX concentration decreased significantly (from 14.62 ± 0.44 μg/L to 0.65 ± 0.07 μg/L). The best growth situation of lettuce and removal rates of NH4+-N, NO3--N, TN, especially LVFX (up to 95.65 ± 0.54 %) were observed in the activated carbon treated group. The overall results indicate the negative effect of residual antibiotics on the hydroponic vegetable systems, and adding activated carbon as substrate is an effective strategy for supporting plant growth and controlling discharged risk.

Edge sites regulation, strain and electric field effect on MoS2/CoS2 heterojunction catalysts for hydrogen evolution reaction.

Heterojunction catalysts in the field of hydrogen evolution reaction (HER) from electrocatalytic water splitting have recently become a hot research topic. In this paper, we systematically calculated the HER catalytic performance of a MoS2/CoS2 heterojunction for the first time, considering the effect of edge sites regulation, strain and electric field. The results indicate that the MoS2/CoS2 heterojunction exhibits synergistic catalytic performance compared to MoS2 and CoS2, the HER catalytic activity of which can be improved by exposing more edge sites or regulating the S content on the edges, with an optimized ratio of 25%. Surprisingly, applying strain has a slight effect on the catalytic activity of the edge, however, an obvious effect on the basal plane. For example, applying 2% tensile strain on the MoS2/CoS2 heterojunction can improve the edge catalytic performance by 13%, and for the basal plane, this value can reach 92%. In this case, the catalytic performance of the basal plane is better than that of the edge with 2% and without strain. Since the basal plane accounts for the majority of the two-dimensional catalysts, the catalytic performance of the basal plane is generally much lower than that of the edge. This discovery is of great significance, which means by adjusting strain, the catalytic performance of the heterojunction catalyst is likely to be improved by orders of magnitude. Moreover, considering the actual experimental process, we also calculated the effect of the electric field and found that 0.7 V/Å electric field can enhance the HER catalytic activity of the MoS2/CoS2 heterojunction by 23%.

The Impact of Urbanization on Surface Runoff and Flood Prevention Strategies: A Case Study of a Traditional Village

Increasingly severe flooding disasters have caused heavy casualties and property losses worldwide. Traditional Chinese villages that rarely experienced flooding disasters in the past have begun to frequently suffer from floods due to unreasonable reconstruction activities such as ground hardening and pond filling caused by urbanization. However, previous studies on hydrological changes and flood disasters caused by reconstruction activities in rural areas are scarce, especially lacking in quantitative analysis and research. In this study, the Storm Water Management Model (SWMM) is used to construct two hydrological models before and after the reconstruction of Hezhai Village, a traditional Chinese village. By simulating and comparing the changes in hydrological indicators of the two models, this study quantitatively analyzes how reconstruction activities caused changes in surface runoff and flooding disasters in Hezhai Village. The results show that the increase in the impervious ratio in the village has obvious effects on the total runoff, peak runoff, and runoff coefficient. And the reconstruction of ponds and canals has a notable impact on flooding. This study further delves into the logic of flooding at ponds and ordinary nodes and analyzes the specific reasons for flooding in Hezhai Village. Based on this, the paper provides recommendations for the optimization of the reconstruction of Hezhai Village.

Structural behavior of partially confined circular CFST columns under lateral cyclic loading

The seismic performance of concrete-filled steel tubular (CFST) columns is severely influenced by the failure at the potential plastic hinge zone. This paper proposes a novel confinement technique at the potential plastic hinge region using an inner circular steel tube for the circular CFST columns named partially confined concrete-filled steel tubular (PCCFST) columns. This confinement technique strengthens the plastic hinge region, and hence, the overall structural performance of the columns is enhanced. To verify the effectiveness of the proposed technique, a total of 7 columns: 6 PCCFST and 1 counterpart CFST have been tested under lateral cyclic loadings while subjected to constant axial load. Failure mode, hysteretic behavior, flexural capacity, ductility, and energy dissipation ability of the PCCFST specimens were investigated by varying the diameter and height of the inner circular steel tube. It was seen that the height of the circular inner steel tube has an obvious effect on the cyclic performance of the columns. For the PCCFST columns considered, the maximum lateral strength, ductility, and energy dissipation ability were enhanced by a maximum of ∼ 16 %, 24 %, and 14 %, respectively, when compared with the counterpart CFST column. A new design guideline has been proposed based on the Eurocode and limited experimental data obtained from the present study. A comparison between the experimentally determined maximum lateral strength and the proposed guideline has been made and is found to be in good agreement.

Investigation of the Time-Dependent Deformation of Recycled Aggregate Concrete in a Water Environment.

The water environment greatly affects the creep deformation of recycled aggregate concrete (RAC). Hence, a humidity-stress-damage coupling numerical model was used for investigating the time-dependent deformation of RAC in the water environment in this study. Firstly, uniaxial compression and water absorption tests were performed to determine the calculation parameters of the creep numerical simulation of RAC in a water environment. Experimental results indicate that the elastic modulus and compressive strength drop as the water content increases. Then, the time-dependent deformation of RAC in a water environment was studied using a numerical simulation test of compressive creep when multiple stress levels were applied, and the critical stress for accelerated creep and the long-term strength of RAC were obtained. Finally, the influence of confining pressures on the long-term deformation of RAC in a water environment was discussed. When there is no confining pressure, the long-term strength of RAC is 23.53 MPa. However, when a confining pressure of 3.921 MPa is loaded onto RAC, the long-term strength of RAC is 47.052 MPa, which increases by 100%. Increasing confining pressures has an obvious effect on ensuring the long-term stable application of RAC in a water environment. Compared with the creep test, the method adopted in this study saves time and money and provides the theoretical basis for evaluating the time-dependent deformation of RAC in a water environment.

Transcriptomics-based anti-tuberculous mechanism of traditional Chinese polyherbal preparation NiuBeiXiaoHe intermediates.

Integrated traditional Chinese medicine and biomedicine is an effective method to treat tuberculosis (TB). In our previous research, traditional Chinese medicine preparation NiuBeiXiaoHe (NBXH) achieved obvious anti-TB effects in animal experiments and clinical practice. However, the action mechanism of NBXH has not been elucidated. Peripheral blood mononuclear cells (PBMCs) were collected to extract mRNA and differentially expressed (DE) genes were obtained using gene microarray technology. Finally, GEO databases and RT-qPCR were used to verify the results of expression profile. After MTB infection, most upregulated DE genes in mice were immune-related genes, including cxcl9, camp, cfb, c4b, serpina3g, and ngp. Downregulated DE genes included lrrc74b, sult1d1, cxxc4, and grip2. After treatment with NBXH, especially high-dose NBXH, the abnormal gene expression was significantly corrected. Some DE genes have been confirmed in multiple GEO datasets or in pulmonary TB patients through RT-qPCR. MTB infection led to extensive changes in host gene expression and mainly caused the host's anti-TB immune responses. The treatment using high-dose NBXH partially repaired the abnormal gene expression, further enhanced the anti-TB immunity included autophagy and NK cell-mediated cytotoxicity, and had a certain inhibitory effect on overactivated immune responses.

Flavokawain B is an effective natural peroxisome proliferator-activated receptor γ-selective agonist with a strong glucose-lowering effect

Rosiglitazone, a full PPARγ agonist and a classical insulin sensitizer, was once used as a powerful weapon in the treatment of T2DM. However, its applications have been restricted recently because of its multiple side effects. Here, a natural compound, flavokawain B (FKB), which was screened in our previous experiments, was investigated for its potential as a preferable insulin sensitizer because it has no or few side effects. Using the surface plasmon resonance (SPR) technique, we confirmed that FKB is a natural ligand for PPARγ with high binding affinity. In in vitro experiments, FKB significantly increased 2-NBDG uptake in HepG2 and 3T3-L1 cells, which partially stimulated PPARγ transcriptional activity. Compared with rosiglitazone, FKB had little effect on the adipose differentiation of 3T3-L1 cells, and all of these features suggest that FKB is a selective modulator of PPARγ (SPPARγM). Moreover, FKB increased the mRNA expression levels of most genes related to insulin sensitivity and glucose metabolism but had no obvious effect on those related to adipose differentiation. In vivo experiments confirmed that FKB effectively decreased abnormal fasting blood glucose and postprandial blood glucose levels and reduced glycated hemoglobin levels, similar to rosiglitazone, in HFD-fed/STZ-treated and db/db mice, two T2DM animal models, but did not cause side effects, such as weight gain or liver or kidney damage. Further investigation revealed that FKB could inhibit PPARγ-Ser273 phosphorylation, which is the key mechanism involved in improving insulin resistance. Together, FKB is a well-performing SPPARγM that exerts a powerful glucose-lowering effect without causing the same side effects as rosiglitazone, and it may have great potential for development.

Preparation and Biological Evaluation of Porous Tantalum Scaffolds Coated with Hydroxyapatite.

Orthopedic implants, such as porous scaffolds, are an effective way to repair bone defects. However, the lack of osseointegration and osteoinduction limits the achievement of an ideal therapeutic effect. This study aimed to prepare hydroxyapatite (HA) coatings for the surface of porous tantalum (Ta) scaffolds and to assess the effectively improved biological activities of the coated scaffolds. The porous Ta scaffolds were prepared by chemical vapor deposition, and then the porous Ta scaffolds were coated with HA via electrochemical deposition. The elements and phase compositions of the coatings were analyzed by energy-dispersive X-ray spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The results showed that the coating covered the whole surfaces of porous Ta scaffolds with a uniform and compact distribution and did not exert any obvious effect on the porous structure. The biological activity of porous Ta scaffolds after surface modification increased and the water contact angle decreased, indicating that hydrophilicity was significantly improved. Cell live/dead staining, cytoskeletal fluorescence staining, and alkaline phosphatase immunofluorescence staining showed that the coating exhibited no cytotoxicity and notably improved cell proliferation, spreading, and osteogenic differentiation. In addition, in vivo experiments in animals have demonstrated that HA-coated porous Ta scaffolds contribute to bone formation. In conclusion, the HA coating notably improves the biological activities of the porous Ta scaffolds, achieving the goal of the present study. The HA coating presents great potential for the modification of porous Ta implants to improve their osteogenesis and osseointegration.

PSD-95 Protein: A Promising Therapeutic Target in Chronic Pain.

Chronic pain, as a social public health problem, has a serious impact on the quality of patients' life. Currently, the main drugs used to treat chronic pain are opioids, antipyretic, and nonsteroidal anti-inflammatory drugs (NSAIDs). But the obvious side effects limit their use, so it is urgent to find new therapeutic targets. Postsynaptic density (PSD)-95 protein plays an important role in the occurrence and development of chronic pain. The over-expression of the PSD-95 protein and its interaction with other proteins are closelyrelated to the chronic pain. Besides, the PSD-95-related drugs that inhibit the expression of PSD-95 as well as the interaction with other protein have been proved to treat chronic pain significantly. In conclusion, although more deep studies are needed in the future, these studies indicate that PSD-95 and the related proteins, such as NMDA receptor (NMDAR) subunit 2B (GluN2B), AMPA receptor (AMPAR), calmodulin-dependent protein kinase II (CaMKII), 5-hydroxytryptamine 2A receptor (5-HT2AR), and neuronal nitric oxide synthase (nNOS), are the promising therapeutic targets for chronicpain.

MEK inhibitor trametinib combined with PI3K/mTOR inhibitor BEZ-235 as an effective strategy against NSCLC through impairment of glucose metabolism

The MAPK and PI3K/AKT/mTOR pathways are aberrantly activated in non-small cell lung cancer (NSCLC) patients, but therapeutic efficacy of NSCLC using trametinib (MEK inhibitor) or BEZ-235 (dual PI3K/mTOR inhibitor) alone is still unsatisfactory. Therefore, in this study, we aimed to determine whether the combination of trametinib with BEZ-235 exerted synergistic effects against NSCLC in both in vitro and in vivo models, and we preliminarily explored the effect of this combination therapy on glucose metabolism. Our results showed that trametinib combined with BEZ-235 could better inhibit cell proliferation and colony formation, induce G0/G1 phase arrest and apoptosis, and suppress cell invasion and migration compared with the single agent. The combination index demonstrated that trametinib and BEZ-235 exerted strong synergistic effects. Additionally, trametinib and BEZ-235 exhibited synergistic antitumor effects in vivo. Furthermore, trametinib and BEZ-235 synergistically downregulated the expression of related proteins in the MAPK and PI3K/AKT/mTOR pathways, and decreased glucose consumption and lactic acid production through suppressing the expressions of glucose transporter 1 (GLUT1) and lactate dehydrogenase A (LDHA). These data imply that simultaneous inhibition of the MAPK and PI3K/AKT/mTOR pathways using trametinib combined with BEZ-235 could synergistically impair glucose metabolism, resulting in an obvious synergistic therapeutic effect against NSCLC.