Remote sensing (RS) platforms such as unmanned aerial vehicles (UAVs) represent an essential source of information in precision agriculture (PA) as they are able to provide images on a daily basis and at a very high resolution. In this framework, this study aims to identify the optimal level of nitrogen (N)-based nutrients for improved productivity in an onion field of “Cipolla Rossa di Tropea” (Tropea red onion). Following an experiment that involved the arrangement of nine plots in the onion field in a randomized complete block design (RCBD), with three replications, three different levels of N fertilization were compared: N150 (150 kg N ha−1), N180 (180 kg N ha−1), and e N210 (210 kg N ha−1). The crop cycle was monitored using multispectral (MS) UAV imagery, producing vigor maps and taking into account the yield of data. The soil-adjusted vegetation index (SAVI) was used to monitor the vigor of the crop. In addition, the coverage’s class onion was spatially identified using geographical object-based image classification (GEOBIA), observing differences in SAVI values obtained in plots subjected to differentiated N fertilizer treatment. The information retrieved from the analysis of soil properties (electrical conductivity, ammonium and nitrate nitrogen), yield performance and mean SAVI index data from each field plot showed significant relationships between the different indicators investigated. A higher onion yield was evident in plot N180, in which SAVI values were higher based on the production data.