Introduction: Obesity in children has been on the rise globally, and India is no exception. Rapid urbanisation, changing dietary patterns, and sedentary lifestyles are contributing factors to this alarming trend. Understanding the prevalence and factors associated with childhood obesity in urban areas is crucial for developing targeted interventions and public health strategies. Aim: To investigate the effects of a nurse-led yoga intervention on body composition and biochemical parameters in overweight and obese adolescents (11-13 years) in schools. Materials and Methods: This pilot randomised controlled study used a true experimental study design, specifically the pretest and post-test control group design. Data collection was conducted in schools of Puducherry. A total of 18 overweight and obese adolescents, nine in the experimental group and nine in the control group, were included. The experimental group performed a nurse-led yoga intervention three times a week for 60 minutes for six months from March 2022 to August 2022. Study outcomes on body composition and biochemical parameters such as lipid profile, adiponectin, leptin, Apolipoprotein A, and Apolipoprotein B were measured at baseline and six months after the nurse-led yoga intervention. Data analysis was performed using Social Sciences Software {Statistical Package for the Social Sciences (SPSS) version 25.0}. Both descriptive and inferential statistical tests, such as the one-way Analysis of Variance (ANOVA) F-test, repeated measures ANOVA F-test, and paired t-test, were conducted. Results: A significant difference was noted in fat percentage, water, intracellular fluid, extracellular fluid, and body fat mass index between the pretest and post-test (p<0.05), and no significant difference was observed in Basal Metabolic Rate (BMR) and fat-free mass index (p>0.05) in the experimental group. The nurse-led yoga intervention significantly improved Lowdensity Lipoproteins (LDL), Apolipoprotein A, and adiponectin levels; however, there were no significant differences in Fasting Blood Sugar (FBS), total cholesterol, High-density Lipoproteins (HDL), Apolipoprotein B, or LDL in the experimental group. The control group showed no significant difference between the pretest and post-test in FBS, total cholesterol, LDL, HDL, Apolipoprotein A, or Apolipoprotein B (p>0.05). Conclusion: The nurse-led yoga intervention positively impacted body composition and biochemical indicators in overweight and obese adolescents.