Oat Varieties Research Articles

Effect of spraying with different concentrations of the growth regulator paclobutrazol on the yield characteristics of three varieties of oat crop Avena sativa L.

The experiment was conducted at the Research Station of the College of Agriculture, University of Basrah,located north of Basrah city, during the winter season of 2022-2023, in a loamy soil. The study aimed toinvestigate the response of three varieties of oats (Avena sativa L.) to foliar spraying with differentconcentrations of the growth regulator paclobutrazol. The study included three oat varieties (Ganzania,Shafa, and CarlIoup) under the influence of spraying three concentrations of paclobutrazol (25, 50, and100 mg/L) and a control treatment. The interaction between the two factors was also studied. Thespraying was carried out in two stages, the first at the beginning of branching, and the second after threeweeks from the first spray.The experiment was arranged using a randomized complete block design(RCBD) with three replicates. The results showed the superiority of the paclobutrazol treatment (50 mg.l-1), which recorded the highest averages for most of the studied yield characteristics, as the lowestaverage number of panicles m-2was recorded at 458.05 dalia - and the highest average number of grainsper dalia was 55.45 grains g-1, and the highest average weight was recorded. 1000 grains amounted to37.33 g, while the Ganzania variety was superior in terms of the number of days and the number ofgrains in of panicl and 1000 grains amounted to 458.05 and amounted to 55.45 grains in of panicl-1 and 34.93 g and its components, protein content in grains, and chlorophyll in leaves were studied. Theresults showed the superiority of the paclobutrazol treatment (50 mg/L), which recorded the highestmeans for most of the studied growth traits. It had the lowest average plant height, reaching 70.75 cm. Italso recorded the highest average chlorophyll content, reaching 143.90 mg/100 g, and the highest averagefor the number of tillers, reaching 488.97 tillers/m². Additionally, it recorded the highest average for stemdiameter, reaching 4.49 mm.

Implementing multi-trait genomic selection to improve grain milling quality in oats (Avena sativa L.).

Oats (Avena sativa L.) provide unique nutritional benefits and contribute to sustainable agricultural systems. Breeding high-value oat varieties that meet milling industry standards is crucial for satisfying the demand for oat-based food products. Test weight, thins, and groat percentage are primary traits that define oat milling quality and the final price of food-grade oats. Conventional selection for milling quality is costly and burdensome. Multi-trait genomic selection (MTGS) combines information from genome-wide markers and secondary traits genetically correlated with primary traits to predict breeding values of primary traits on candidate breeding lines. MTGS can improve prediction accuracy and significantly accelerate the rate of genetic gain. In this study, we evaluated different MTGS models that used morphometric grain traits to improve prediction accuracy for primary grain quality traits within the constraints of a breeding program. We evaluated 558 breeding lines from the University of Illinois Oat Breeding Program across 2 years for primary milling traits, test weight, thins, and groat percentage, and secondary grain morphometric traits derived from kernel and groat images. Kernel morphometric traits were genetically correlated with test weight and thins percentage but were uncorrelated with groat percentage. For test weight and thins percentage, the MTGS model that included the kernel morphometric traits in both training and candidate sets outperformed single-trait models by 52% and 59%, respectively. In contrast, MTGS models for groat percentage were not significantly better than the single-trait model. We found that incorporating kernel morphometric traits can improve the genomic selection for test weight and thins percentage.

Estimation of adaptability parameters of collection hulled oats varieties according to productivity in the Kirov region

The current paper has presented the study results conducted on the experimental plot of the Falenskaya breeding station of the branch of the FSBI FANC of the North-East in 2021–2023. The purpose of the study was to evaluate the collection hulled oat varieties according to grain productivity and adaptability parameters in the soil and climatic conditions of the Kirov region. The materials for the study were 9 collection oat varieties and the standard variety ‘Krechet’. According to the trait ‘productivity’, there have been estimated adaptability parameters. During the testing years, the studied variety samples differed significantly in productivity variability (V = 16.8–30.0 %). The factor ‘year’ had the greatest effect on the formation of oat yields (90.3 %). In the arid year of 2021, the varieties’ productivity was low (276–401 g/m2), with a negative index of environmental conditions (Ij = -163), in the most favorable year of 2022, it was 463–682 g/m2 and Ij = 104, respectively. The variety samples ‘k-3967 0144’ (-190 g/m2) and ‘15584 Mutant 258’ (-214 g/m2) were characterized by high stress resistance. High genetic flexibility of mean productivity under contrasting conditions was established in the variety samples ‘15472 Sumphony’ (504 g/m2), ‘k-3967 0144’ (496 g/m2) and ‘15340 Uran’ (487 g/m2). There have been identified such gene sources as plastic ‘k-3967 0144’, ‘15472 Sumphony’, ‘15340 Uran’, ‘k-3951 0146’, ‘k-3954 0149’ (IEP = 1.13–1.0), and with high stability of the trait ‘productivity’ ‘k-3967 0144’, ‘15584 Mutant 258’, ‘k-3954 0149’ (SF = 1.47–1.78). Low variability and high homeostaticity were found in the variety samples ‘k-3967 0144’, ‘15584 Mutant 258’, ‘k-3954 0149’ (V = 16.8–22.8 %; Hom = 31.31–20.75) with a high stability index (Is = 5.95–4.38). The adaptability coefficient has confirmed the high adaptability of variety samples ‘k-3967 0144’, ‘15472 Sumphony’ and ‘15340 Uran’ (KA =112.3–108 %). In adaptive breeding for further work, the varieties ‘k-3967 0144’, ‘15584 Mutant 258’, ‘k-3954 0149’, ‘15472 Sumphony’, ‘15340 Uran’ are of great interest, since they are the most adaptive in terms of the sum of ranks. Based on a comprehensive estimation of productivity and adaptability parameters, ‘k-3967 0144’ was recognized as the best variety sample, which can give a large mean productivity (526 g/m2) in changing weather conditions.

Exploring the genetic variability in yield, nutritional and digestibility traits in oat grains through ruminant nutrition

Oat is a dual-purpose crop used for both food and feed for animals. The objective of this work is to characterize oat varieties for their genetic diversity in yield, physical traits, and nutritional composition, aiming to identify potential parent varieties for breeding programs to develop new oat varieties for improved livestock feed and diverse industrial applications. To conduct, chemical analysis for protein and carbohydare fractions, energy and digestible nutrient estimated, stastical analyses performed to assess genetic variations for traits among vaieties. Significant genetic variation (p < 0.05) for grain yield, grain density, sieving percentage, crude protein, ether extract, neutral and acid detergent fiber, cellulose, lignin, neutral and acid detergent insoluble nitrogen were observed in grains of eight oat varieties. All protein fractions exhibited significant differences (p < 0.05). Total carbohydrate content ranged significantly (p < 0.05) from 73 % to 79 %. The grains contained higher levels of intermediately degradable starch and pectin (54.12–60.16 %) compared to the slowly degradable cell wall (26–33 %), lignin bounded cell wall (6–10 %), and rapidly degradable sugars (2–8%). Significant variation (p < 0.05) was observed in terms of gross energy, digestible energy, metabolizable energy, net energy for maintenance and lactation about (2 Mcal/kg dry matter), gain (1.6–1.8 Mcal/kg dry matter), total digestible nutrients, digestible dry matter, rumen degradable protein, and total digestible nutrients related to crude protein, fatty acid, neutral detergent fiber, and non-fiber carbohydrate. Organic matter and ether extract were positively associated (p < 0.01) with total digestible nutrients, digestible and metabolizable energy, dry matter digestible and truly digestible non fibrous cabohydrates, while neutral and acid detergent fiber and cellulose showed negative correlation. The research shows that oat varieties vary widely in their yield, physical features, and nutritional content, offering potential for breeding better varieties for both animal feed and industrial uses.

Effects of fertilizer levels and varieties on fodder yield productivity, nutrient use efficiency, and profitability of oat (Avena sativa L.) in the central highlands of Ethiopia

This study aimed to assess the effect of fertilizer rates on agro-morphological traits, nutrient uptake and use efficiency, and economic feasibility of fodder yield in oat. The study was carried out using a factorial arrangement of eight fertilizer rates (0, 25, 50, 75, 100, 125, 150, and 175 %) and three oat varieties (CI-8251, SRCPX80Ab2291, and Sorataf) in a randomized complete block design with three replications. The results revealed that variety, fertilizer, and year had a significant effect on fodder yield. The fodder yield increased up to 175 % of the blanket recommended fertilizer and application of 100, 125, 150, and 175 % produced fodder yield of 14.0, 15.6, 15.8, and 15.9 t/ha, respectively. The fodder type oat (CI-8251) and dual type oat (SRCPX80Ab2291) variety had 15.4 and 10.6 % fodder yield advantages over grain type oat (Sorataf), respectively. Significantly higher fodder yield was obtained in 2021 than 2020 cropping season. Moreover, the interaction effect of variety by fertilizer by year was significant for nitrogen and phosphorus concentrations, nitrogen and total nutrient uptake, partial factor productivity, internal utilization efficiency, agronomic efficiency, and agro-physiological efficiency. The Pearson correlation analysis indicated that the number of tillers per plant, yields of morphological fractions (leaf, stem, and panicle), forage yield production rate, nutrient concentration, and uptake had significant positive associations with fertilizer levels and fodder yield. In contrast, all nutrient use efficiency except agro-physiological efficiency had inverse associations with fertilizer levels and fodder yield. The combined partial budget analysis for the three oat varieties showed that the application of 125 % fertilizer produced a higher net benefit (83,440 ETB) and marginal rate of return (12,635.6 %). Therefore, the blanket recommended fertilizer rate of 125 % (51.3 kg N + 25 kg P/ha) could increase fodder yield and farm profitability in the study area.

Study of genetic variations of oat varieties under insufficient and insufficient irrigation

A field experiment was carried out in the fields belonging to the Faculty of Agricultural Engineering Sciences /University of Baghdad-Jadriya during the season 2020-2021 in order to study the genetic variations of varieties of oats under lack of irrigation. The experiment was carried out using the design of complete random sectors and the distribution of splintered panels and two factors, the first was the treatment of irrigation at the arrival of humidity ( 50%, 25% and 10%), while the second factor was the varieties (Genzania, Anatolia, plmulao Algoda and healing ) the experiment was carried out three times. The results showed that the third irrigation level exceeded the number of days of planting up to 75% flowering in the second irrigation treatment gave the highest averages for all other qualities studied, and the V4 variety gave the highest average for all qualities except for the weight of 250 grains. . While there was a significant significant difference for the second irrigation level in the values of genetic variation for all accuracy except for the single trait, while we note that accuracy is the number of branches and the total number of branches only and it was highly effective at the first irrigation level. Later, the effect was good at the second irrigation level. The difference is in appearance. It is noted that the values at the second level of road types, except for the obtained characteristic and the nature ratio, gave the second level of irrigation the highest average for most types of resistance. Why is it not different from what was mentioned above, which did not adopt a study of scientific variations in the expected researchers, as you would expect, the second irrigation and the V4 variety, which recorded the highest averages for the characteristics that were studied.

Nutritional Traits, Pasting Properties and Antioxidant Profile of Selected Genotypes of Sorghum, Oat and Maize Eligible for Gluten-Free Products.

The technological and nutritional traits of food-grade sorghum hybrids, hulled/naked oat varieties and maize genotypes of different colors were studied for novel and healthier gluten-free foods. Oat genotypes showed the highest protein content, followed by maize and sorghum. The total starch and the total dietary fiber content were quite similar among the three species. Great variation was found in the amylose content, and the highest was in sorghum (27.12%), followed by oat 16.71% and maize 10.59%. Regarding the pasting profile, the rank of Peak Viscosity was sorghum (742.8 Brabender Unit, BU), followed by maize (729.3 BU) and oat (685.9 BU). Oat and sorghum genotypes had similar average breakdown (407.7 and 419.9 BU, respectively) and setback (690.7 and 682.1 BU, respectively), whereas maize showed lower values for both parameters (384.1 BU and 616.2 BU, respectively). The total antioxidant capacity, only in maize, significantly correlated with total flavonoid, phenolic and proanthocyanidin contents, indicating that all the measured compounds contributed to antioxidant capacity. The study indicated the importance of sounding out the nutritional and technological characteristics of gluten-free cereals in order to select suitable cultivars to be processed in different gluten-free foods with better and healthier quality.

Change and influence of agroclimatic conditions on oat yield in Yakutia

The results of the dynamics analysis of multi-year meteorological indicators and study of their influence on the yield of the released oat varieties in different agroclimatic zones of Yakutia are presented. It is shown that in the period from 1960 to 2021 the mean annual air temperature in Central Yakutia (Pokrovsk meteorological station) increased annually by 0.045 °C, from 1999 to 2021 – by 0.088 °C, the number of dry years in June increased from 67 to 92% due to a 21% increase in years with medium (HTC = 0.41-0.60) and very strong (HTC &lt; 0.21) drought. In July, drought was recorded in 50% of the years, in August – in 38%. In all agroclimatic zones in the period from 2014 to 2021 for June – August the average daily air temperature annually increased by 0.16...0.34 °С and their sum by 14...28 °С, with precipitation decreasing by 3-16 mm and HTC value by 0.04-0.12 units. Climate change from 2014 to 2021 was 3 to 8 times more intense than from 1999 to 2022. There was a strong variability of yields of the released oat varieties in each agroclimatic zone: Central – from 0.38 to 2.14 t/ha (V = 60–73%), Zarechnaya – from 0.31 to 3.55 t/ha (V = 41–49%), Srednelenskaya – from 1.51 to 4.66 t/ha (V = 30–36%). The greatest annual decrease in grain yield of oat varieties (by 0.25–0.26 t/ha) was observed in arid agroclimatic zones – Central (Yakutsk SCTS) and Zarechnaya (Megino-Kangalassky SCTS) – compared to the wetter Srednelenskaya zone (Olyokminsky SCTS – by 0.10 t/ha). More significant influence on the yield in the Central and Zarechnaya zones was made by precipitation and HTC of the growing season (r = 0.77...0.84), in the Srednelenskaya zone – by temperature (r = – 0.79). Negative effect of temperature on the yield in the Central zone was observed in June (r = – 0.83), Zarechnaya zone - in July (r = – 0.83), in the Srednelenskaya zone – in August (r = –0.86). Precipitation and HTC had the greatest influence on the yield in the Central zone in June and July (r = 0.56...0.59), Zarechnaya zone – July and August (r = 0.60...0.76). In the Srednelenskaya zone the relationship of the yield with precipitation and HTC in June and July was positive (r = 0.23...0.37), in August – negative (r = – 0.57...– 0.71). Significant variability in grain yields of the released oat varieties indicates the variability of weather conditions and the need to cultivate oat varieties that provide a more stable yield in different agroclimatic zones of Yakutia.

Assessment of stability and plasticity of hulless spring oat varieties by yield and 1000 seed weight in the Northern Russia

Assessment of stability and plasticity of hulless spring oat varieties by yield and 1000 seed weight in the Northern Russia

Enhancing Soil Health and Sustainability: The Impact of Melia Dubia-based Agroforestry in a Semi-Arid Region of Haryana, India

This study investigates the impact of a Melia dubia-based agroforestry system on soil properties in Gillan Khera, Fatehabad district, located in the semi-arid region of Haryana. Soil samples were collected from a 7-year-old plantation with a 3m × 3m spacing, where three oat varieties were intercropped. Parameters such as pH, electrical conductivity (EC), organic carbon, soil moisture, and available nitrogen, phosphorus, and potassium were analysed at depths of 0-15 cm and 15-30 cm. Results revealed a decrease in soil pH and EC under trees, with values decreasing from 8.09 to 7.89 and 0.46 to 0.44 dSm-1, respectively. However, intercropped conditions exhibited higher levels of nitrogen (131.38 kg/ha), phosphorus (16.00 kg/ha), potassium (301.10 kg/ha), and organic carbon (0.46%) at both soil depths. Additionally, there was more soil moisture under the plantation. These findings suggest a positive correlation between tree growth and soil health. The study recommends the Melia dubia-based agroforestry system as a promising approach for enhancing soil fertility and promoting environmental sustainability.

ВЛИЯНИЕ КОЛИЧЕСТВА СКАШИВАНИЙ НА ЭНЕРГОСОДЕРЖАНИЕ И ЭНЕРГОПРОДУКТИВНОСТЬ ЯРОВОГО ОВСА В КРАСНОЯРСКОЙ ЛЕСОСТЕПИ

The purpose of the study is to establish the influence of the method of use on the energy content and energy productivity of spring oat varieties. Objectives: to study the effect of the number of mowing on the energy content and dry matter collection; determine the dependence of energy productivity on the method of use, weather conditions and oat variety. The study was carried out in the forest-steppe zone of the Krasnoyarsk Region. Two cultivation methods were studied: single-cut (traditional technology with combine harvesting) and double-cut. The double-mowing method of use was carried out by mowing the green mass of oat varieties during the booting phase, and after their regrowth and the formation of a grain harvest, harvesting it. The yield of green mass was determined by a continuous method, by weighing the entire mass from the accounting area of the plot. Accounting, observations, and calculation of energy productivity were carried out according to the methodology of the All-Russian Research Institute of Feeds named after V.R. Williams. The objects of the study are oat varieties included in the list of breeding achievements approved for use in the Krasnoyarsk Region and promising for use: Tubinsky, Sig, Sayan, Uzhursky, Ural 2, Krasnoobsky and Poloves. Control is single cutting use and Tubinsky variety. The energy content depended on the method of use and type of product. The energy content of dry matter of green mass ave¬raged 9.681 MJ/kg; energy content of grain with double mowing – 13.003; with a single mowing – 13.515 MJ/kg. In the forest-steppe conditions of the Krasnoyarsk Region, the energy content of oat varieties corresponded to the first quality class. In terms of energy content of dry matter of green mass, the Tubinsky variety Sayan was superior to the control. The dry matter energy content of this variety averaged 11.133 MJ/ha. The energy productivity of oats depended on the variety, weather conditions of the years of research and energy content. In terms of energy productivity, all studied varieties showed an advantage when used with double-cutting compared to single-cutting. The maximum increase in single cutting use was shown by the Sig variety, which exceeded the control by 127.1 %. The Sayan variety stood out in terms of energy productivity; its increase per cutting was 60.2 GJ/ha.

Registration of the Oat (Avena sativa) Variety "Walqaa" for Vertisols in the Central Highlands of Ethiopia

In this study, fifteen oat varieties including the standard checks were evaluated for agro-morphological traits, nutritional qualities, disease, and insect pest reactions during the main cropping seasons of 2015- 2018. Based on the overall performance, two best-performing oat varieties (CI-1742 and SRCPX80Ab2596) were selected and verified with the two standard checks at the Kuyu and Ginchi sub-site of Holetta Agricultural Research Center during the main cropping season of 2018. The overall mean result indicated that the released variety, Walqaa (SRCPX80Ab2596), showed the highest mean DM yield followed by the recent check (SRCPX80Ab2291) while the check variety (CI-8251) which was released earlier and commonly used for vertisol gave the lowest DM yield. Walqaa (SRCPX80Ab2596) had the highest percent increase in DM yield, CP yield, and digestible yield advantages over the standard check variety (CI-8251) which was released earlier and commonly used for vertisol conditions. Similarly, the Walqaa (SRCPX80Ab2596) had a relatively better percent increase in DM yield, CP yield, and digestible yield advantages over the recently released variety (SRCPX80Ab2291). Moreover, the Walqaa (SRCPX80Ab2596) had seeds yield advantage over the earlier released variety (CI-8251). The nutritional qualities indicated that the candidate varieties had advantages over the standard check variety (CI-8251) in terms of the leafto-stem ratio, CP, and IVDMD contents. Generally, the Walqaa (SRCPX80Ab2596) had a relatively better leaf-tostem ratio, CP, and IVDMD advantages over the standard check variety (CI-8251). The national variety releasing committee evaluated the varieties at field conditions in October 2018. Based on their evaluation result, oat variety Walqaa (SRCPX80Ab2596) was officially released in November 2019 for production in the high altitude areas and similar agro-ecologies of the country. The pre-basic and basic seeds of the released variety Walqaa (SRCPX80Ab2596) are maintained by the feeds and nutrition research program of Holetta Agricultural Research Center. Keywords: Oat, quality, variety release, variety verification, yield

Effect of kilning on the macronutrient composition profile of three Swedish oat varieties

AbstractBackground and ObjectivesKilning is crucial in oat processing, to prevent rancidity and extend shelf‐life. This study examines kilning effects on the macronutrient composition in Swedish oat varieties Galant, Fatima, and Belinda. We compared two kilning methods: one mimicking industrial practice and a simplified version. We analyzed dietary fibers (arabinoxylan, β‐glucan), protein and amino acids, lipid profile, lipase activity, and antioxidant capacity in these oat samples.FindingsDistinct compositional differences were found: Galant had low lipid content, Fatima had elevated lipid and protein levels with fewer carbohydrates, and Belinda was rich in β‐glucan and dietary fibers. Both kilning procedures had similar impacts on all varieties, causing no major changes in dietary fiber or total protein content, but resulting in a 20% decrease in soluble proteins. Kilning decreased levels of several amino acids in Belinda, while the l‐glutamate/glutamine ratio increased across all varieties. Lipid analysis showed minimal kilning‐induced changes; yet, antioxidative capacity diminished. Both kilning methods effectively inactivated lipases.ConclusionsThese findings emphasize macronutrient variations among oat varieties and the effect of kilning on soluble proteins, amino acids, and antioxidative capacity.Significance and NoveltyThis study underscores the need for precise control in oat processing, crucial in plant‐based protein and novel food development for optimal quality and yield.

Lipidomics studies reveal dynamic changes in polar lipids of developing endosperm of oat and wheat varieties with differing oil contents

Polar lipids have biosynthetic pathways which intersect and overlap with triacylglycerol biosynthesis; however, polar lipids have not been well characterized in the developing endosperms of oat with high oil accumulation. The polar lipids in endosperms of oat and wheat varieties having different oil contents were analyzed and compared at different developmental stages. Our study shows that the relative contents of polar lipid by mass were decreased more slowly in wheat than in oat. Phosphatidylcholine and phosphatidylethanolamine were the major phospholipids, which showed similar abundance and gradual decreases during endosperm development in oat and wheat, while lysophospholipids were noticeably higher in oat. Monogalactosyldiacylglycerol showed a gradual increase in wheat and a decrease in oat during endosperm development. The relative contents of some polar lipid species and their unsaturation index were significantly different in their endosperms. These characteristics of polar lipids might indicate an adaption of oat to accommodate oil accumulation.

Оценка коллекции овса по основным биохимическим показателям качества в условиях Тюменской области

Abstract. Oats are an important source of valuable nutrients, especially protein and fat, with a balanced amino acid composition. This study presents long-term data on the study of the quality of grain of filmy oats in the Tyumen region. The purpose of this study was to evaluate the biochemical potential of various collectible varieties of filmy oats for involvement in the breeding process in the conditions of the Tyumen region. Materials and methods of the research. In 2019–2021, 167 varieties of filmy oats of various ecological and geographical origin were sown on the experimental field of the Northern Trans-Urals Research Institute, Tyumen Region (Russia), using generally accepted methods. Results. The most favorable conditions for the formation of protein in oat grain were 2019 (correlation coefficient r = –0.59) and 2020 (r = –0.34). The correlation of yield with fat content and starch content was not significant. The protein content in oat grain over the years of study was: in 2019 7.87–13.58 %, in 2020 – 8.74–13.33 %, in 2021 – 7.25–14.05 %. According to the protein content in the grain, the following varieties were distinguished: k-15321 (Leningrad region); k-15262 (USA). Fat content in oat grain in 2019 – 4.35 %, in 2020 – 4,00 %, in 2021 – 3,62 %. The most interesting varieties are k-15311 (Leningrad region); k-15353 (Norway); k-15256 (USA); k-15254 (Canada). The starch content in oat grain was in 2019 – 57.63 %, in 2020 – 58.40 %, in 2021 – 51.04 %. Varieties were distinguished: k-15330, k-15331, k-15329 (Ulyanovsk region); k-14863 (Estonia); k-15378 (Germany); k-15307 (USA); k-14953 (Australia). The most interesting are oat varieties that combine a complex of biochemical characteristics: k-15278 (Moscow region); k-15311 (Leningrad region); k-15330, k-15329 (Ulyanovsk region); k-15378 (Germany); k-15307 (USA); k-14953 (Australia). Scientific novelty. A long-term evaluation of 167 varieties of spring oats according to biochemical quality indicators was carried out, the sources that are of the greatest interest for breeding were identified.

Photosynthetic Activity as a Factor of the Yield Formation and Quality of the New Grain Irtysh 33 Fodder Oat Variety

Photosynthetic Activity as a Factor of the Yield Formation and Quality of the New Grain Irtysh 33 Fodder Oat Variety

Белковый азот как показатель определения степени стресса овса при почвенной засухе

Abstract. The purpose is to studying the response of oats to soil drought by the degree of change in the content of protein nitrogen in the leaves and identifying the most drought-resistant varieties for the breeding process. Methods. In the experiment, 40 varieties of oats were used, for which soil drought during tillering and flowering was artificially modeled. Determination of protein content in oat leaves was carried out on the twelfth day after the cessation of irrigation, using the Bradford reagent. Results. The reaction of oats to soil drought in the critical phases of ontogeny (tillering and flowering), expressed as a change in the protein content in the vegetative parts, was studied. On average for the collection, the protein content in tillering was 13.6 %, varying from 10.3 to 16.9 %. In flowering, this indicator decreased to 6.6–14.4 %. It was established that soil drought during the tillering period had a minimal impact on the varieties Assol’, Novosibirskiy 5, Ulov, KROSS, Fakel, Sig, Privet, Megion, Barguzin and Argument. The decrease in protein content in these varieties varied from 7.4 to 10.8 %. In the varieties Pesets, Uran, L’govskiy, Bulanyy, Tigrovyy, Ekspress, Dedal, Narymskiy 943, Pokrovskiy 9, Rovesnik and Borets, soil drought during tillering led to a decrease in the protein content in the leaves by 20.4–30.8 %. The varieties Sig, Ulov, KROSS, Chizh, Novosibirskiy 5, Megion and Fakel were the most resistant to drought during flowering. During the analysis of the data obtained, it was revealed that the varieties L’govskiy 82, Privet, Peredovik, Assol’, Ulov, Novosibirskskiy 5, and Sig reacted to a minimum degree to drought during tillering and flowering. Scientific novelty. The response to drought for 40 oat genotypes was established and the relationship between protein content and plant biomass was determined by the beginning of harvesting. The most promising genotypes for breeding drought-resistant oat varieties have been identified.

Genome-wide analysis and expression of the aquaporin gene family in Avena sativa L.

Oat (Avena sativa L.) belongs to the early maturity grass subfamily of the Gramineae subfamily oats (Avena) and has excellent characteristics, such as tolerance to barrenness, salt, cold, and drought. Aquaporin (AQP) proteins belong to the major intrinsic protein (MIP) superfamily, are widely involved in plant growth and development, and play an important role in abiotic stress responses. To date, previous studies have not identified or analyzed the AsAQP gene family system, and functional studies of oat AQP genes in response to drought, cold, and salt stress have not been performed. In this study, AQP genes (AsAQP) were identified from the oat genome, and various bioinformatics data on the AQP gene family, gene structure, gene replication, promoters and regulatory networks were analyzed. Quantitative real-time PCR technology was used to verify the expression patterns of the AQP gene family in different oat tissues under different abiotic stresses. In this study, a total of 45 AQP genes (AsAQP) were identified from the oat reference genome. According to a phylogenetic analysis, 45 AsAQP were divided into 4 subfamilies (PIP, SIP, NIP, and TIP). Among the 45 AsAQP, 23 proteins had interactions, and among these, 5AG0000633.1 had the largest number of interacting proteins. The 20 AsAQP genes were expressed in all tissues, and their expression varied greatly among different tissues and organs. All 20 AsAQP genes responded to salt, drought and cold stress. The NIP subfamily 6Ag0000836.1 gene was significantly upregulated under different abiotic stresses and could be further verified as a key candidate gene. The findings of this study provide a comprehensive list of members and their sequence characteristics of the AsAQP protein family, laying a solid theoretical foundation for further functional analysis of AsAQP in oats. This research also offers valuable reference for the creation of stress-tolerant oat varieties through genetic engineering techniques.

PERSPECTIVES AND OPPORTUNITIES OF GROATS PRODUCTION IN UKRAINE

Since the beginning of the 21st century, there has been an increase in the share of small enterprises in the grain industry, the number of which is increasing even today. At most of such enterprises, it is impossible to apply the existing longterm full grain processing technologies, there is a reduction in the number of basic technological operations, often not sufficiently substantiated, which affects the quality of the end products, which do not always meet all the basic norms of the regulations applicable to end products. Considering the modern technological solutions offered by leading manufacturers for the production of flaked products and quick-cooking products, it is possible to note the equipment of the Buhler company, which allows to monitor production control and, accordingly, achieve greater production efficiency. To date, the Buhler company offers the batch steamer MBDA for the steaming stage, which is recommended to be combined with the BCFB flaking machine. This technological equipment in combination gives high quality and reliability of the equipment itself, allows to implement energy-saving technologies at enterprises and obtain controlled production of quality products. Today, in addition to traditional grain, such crops as lentils, chickpeas, beans, wheat-spelt, spelt, naked varieties of oats and barley are processed in part or in small batches at the enterprises of the industry. Lentil is a promising leguminous crop for the grain industry. In the world, it is widely grown as a food and fodder crop. Among leguminous crops, lentils occupy an intermediate place in terms of protein content, with a protein content of 25-36%, while the share of carbohydrates is 45-55%, fat - 3-4%. The first variety of «Linza» edible lentils was registered in 2005. After that, the following varieties were registered only in 2017-2018: «Antonina» (2017), «Darinka» (2017), «YeSMaksymum» (2017), «Blondie» (2018), «Harry» (2018), «SNIM 18» (2018), Chrysolite (2018), which indicates an understanding of the potential of this culture both for the domestic market and for increasing the export potential of our country. The last to enter the register were «Serpanok» (2020) and «RED» (2021) varieties, which indicates the continued work of breeders to expand the varietal base of this crop in Ukraine.

Transcriptome Analysis Reveals Drought-Responsive Pathways and Key Genes of Two Oat (Avena sativa) Varieties.

To cope with the yield loss caused by drought stress, new oat varieties with greater drought tolerance need to be selected. In this study, two oat varieties with different drought tolerances were selected for analysis of their phenotypes and physiological indices under moderate and severe soil drought stress. The results revealed significant differences in the degree of wilting, leaf relative water content (RWC), and SOD and CAT activity between the two oat genotypes under severe soil drought stress; moreover, the drought-tolerant variety exhibited a significant increase in the number of stomata and wax crystals on the surface of both the leaf and guard cells; additionally, the morphology of the guard cells was normal, and there was no significant disruption of the grana lamella membrane or the nuclear envelope. Furthermore, transcriptome analysis revealed that the expression of genes related to the biosynthesis of waxes and cell-wall components, as well as those of the WRKY family, significantly increased in the drought-tolerant variety. These findings suggest that several genes involved in the antioxidant pathway could improve drought tolerance in plants by regulating the increase/decrease in wax and cell-wall constituents and maintaining normal cellular water potential, as well as improving the ability of the antioxidant system to scavenge peroxides in oats.