Oak Forests Research Articles

Forest soil CO2 emission in Quercus robur level II monitoring site

Abstract In this study, the soil CO2 emission was analysed at the level II ICP Forests monitoring plot in Serbia in the pedunculate oak forest. Two plots of pedunculate oak (Quercus robur L.) were selected for this study. The main question was to determine the differences in the impact of management (human impact) on CO2 emission. Different time periods were compared to identify the main factors affecting soil CO2 emission. Sampling was done by chambers. During the study period, climate indicators were quite different. A strong positive correlation between the soil temperature and soil CO2 emission, as well as a strong negative correlation between the soil moisture and soil CO2 emission, was found in the spring aspect (Plot). In other cases, a moderate to weak correlation was found. Multiple linear regressions showed that CO2 emission from soil was primarily controlled by soil moisture. Increasing soil water content had a positive effect on soil respiration (except in spring). The effect of soil temperature appeared in the multiple regressions as a secondary factor during the period studied, and an increase in temperature resulted in a decrease in soil respiration (except in spring).

Utilidad de los portales en línea de ciencia ciudadana para estudios florísticos: Orquídeas de Santa María Yucuhiti, Oaxaca, México

Background: The beauty of orchids attracts the interest of users of citizen science portals, turning them into additional sources of information for the study of biodiversity. Currently, iNaturalist records observations of almost 70 % of Mexican orchid species. Here, we evaluate the usefulness of the platform to create a local floristic list of this group of plants. Question: Does the information available on iNaturalist allow for the creation of a local orchid checklist as a conventional floristic study does? Study site and dates: Observations recorded between 2007 and 2024 in Santa María Yucuhiti, Oaxaca, Mexico. Methods: Orchid observations in iNaturalist were reviewed and curated, assigning vegetation type and site elevation for each record. The resulting floristic checklist was commented and compared, using a richness index, with those of other regions of Oaxaca, that were prepared through conventional floristic studies. Results: The study area holds 107 species of orchids, 50 % are endemic to Mexico. The highest richness is found between 1,500-1,999 m elevation and in the oak forest. Acianthera hintonii, Habenaria tetranema and Prosthechea mulasii represent new records for Oaxaca. The study area showed the highest orchid richness index compared to other regions of Oaxaca. Conclusions: Information from iNaturalist allowed the preparation of the orchid checklist for Santa María Yucuhiti, supported by the validated observations on the platform. This, along with the availability of high-resolution photographs, the temporal continuity in documentation, and the exploration of sites with restricted access, enhances the quality of the checklist.

Terrain's steepness governs sensitivity of urban oak forests to climate variability

Terrain's steepness governs sensitivity of urban oak forests to climate variability

Fungal Footprints: Soil Fungal Communities in Black Walnut and Red Oak Forests

Soil fungal communities are critical for forest ecosystem functions in the Central Hardwood Region (CHR) of the USA. This evaluation, which took place in 2022–2023, investigates the influence of Juglans nigra (BW, black walnut) and Quercus rubra (NRO, Northern red oak) on soil properties and fungal community structures across three CHR sites. The objectives of this study are to investigate how the fungal communities identified beneath J. nigra and Q. rubra serve to influence biodiversity and soil health within hardwood plantations. Soils from two locations in Indiana and one in Michigan were examined and assessed for variations in fungal composition and diversity. Soil fungal communities were characterized using Illumina high-throughput sequencing while multivariate analysis was applied to analyze patterns in these fungal communities. These data provided insights into how environment, location, and tree species affect fungal community structure. Results indicate that J. nigra soils exhibited higher carbon (0.36%, 1.02%, 0.72%), nitrogen (25%, 29%, 56%), and pH (0.46, 1.08, 1.54) levels than Q. rubra soils across all three sites and foster greater fungal diversity. Specifically, J. nigra was associated with increased Ascomycota diversity, whereas Q. rubra supported a higher prevalence of Basidiomycota. Basidiomycota were negatively correlated with carbon and pH, while Ascomycota showed positive correlations with these variables. These findings highlight how crucial it is to understand how different tree species influence fungal communities and, consequently, how they influence forest soil health. Our findings serve to improve forest management practices by emphasizing the importance of fungal communities in maintaining the function and resilience of an ecosystem. Our study underscores that grasping these specific interactions is essential for effective forest management, especially when considering how to use fungal communities to boost plant growth. This work focuses on hardwood plantations rather than either agricultural ecosystems, monocultures, or native forests, thus filling a gap in the current literature where many studies are limited to specific fungal groups such as mycorrhizae. In future research, it is important to examine a wider range of tree species. This will deepen our understanding of fungal community dynamics and their impact on maintaining healthy forest ecosystems. Our hardwood plantation focus also notes the potential for adaptive forest management as environmental conditions change.

Topological differentiation of vegetation of the Lublin-Volyn geobotanical district

The position of the Lublin and Volyn (Volhynian) uplands within the geobotanical zonation system is ambiguous and disputable. An analysis of the syntaxonomical diversity and landscape distribution of the egetation of the Lublin and Volyn uplands revealed their similarity, and we consider this whole territory as the Lublin-Volyn geobotanical district of hornbeam-oak, oak forests, and steppe meadows. This district differs from the adjacent territories and is classified within the Central European mixed forests ecoregion. Typical and diagnostic syntaxa have been identified, and ecological-coenotic profiles have been constructed to characterize the distribution patterns of shrub-forest and herbaceous vegetation within the landscape. Based on calculations of phytosociological indicators for the main ecological factors, graphical patterns of their variation were developed. It was determined that the characteristic forests of the district, Tilieto-Carpinetum (Carpinion betulis), are represented by four variants (T.-C. var. galeobdolosum; T.-C. var. hepaticosum; T.-C. var. caricosum pilosae; T.-C. var. isopyrosum). The district is also characterized by the presence of syntaxa associated with carbonate soils, both of the marsh type (Caricion davallianae) and the meadow-steppe type (Cirsio-Brachypodion, Festucion valesiacae), with the dominance of Carex humilis and Stipa capillata. Using DCA-analysis, the correlation between ecological factor indicators and the distribution of plant communities within the ecological space has been established, forming six fields distributed along three vectors: by moisture (aquatic and marsh communities), xerophytism, and chemical properties of soils (meadow-steppe communities), as well as the structure of coenoses with a specific ombroregime (forest communities).

Development of Polymorphic Index Model for Assessing Subtropical Secondary Natural Oak Forest Site Quality Under Complex Site and Climate Variables

Site and climate conditions are the key determinants controlling dominant height growth and forest productivity, both independently and interactively. Secondary natural oak forests are a typical forest type in China, especially in Hunan Province, but little is known about the site index of this forest under the complex site and climate variables in the subtropics. Based on survey data of dominant trees and site variables from 101 plots in Hunan oak natural secondary forests and climate data obtained using spatial interpolation, we used the random forest method, correlation analysis, and the analysis of variance to determine the main site and climate factors affecting oak forest dominant height and proposed a modeling method of an oak natural secondary forest site index based on the random effect of site–climate interaction type. Of the site variables, elevation affected stand dominant height the most, followed by slope direction and position. Winter precipitation and summer mean maximum temperature had the greatest impact on stand dominant height. To develop the modeling method, we created 10 popular base models but found low performance (R2 ranged from 0.1731 to 0.2030). The optimal base model was Mitscherlich form M3 (R2 = 0.1940) based on parameter significance tests. Since site and climate factors affect the site index curve, the dominant site and climate factors were combined into site types and climate types, respectively, and a nonlinear mixed-effects approach was used to simulate different site types, climate types, site–climate interaction types, and their combinations as random effects. Site–climate interaction type as a random factor enhanced model (M3.4) performance and prediction accuracy (R2 from 0.1940 to 0.8220) compared to the optimum base model. After clustering the 62 site–climate interaction types into three, five, and eight groups using hierarchical clustering, a mixed-effects model with the random effects of eight groups improved model performance (R2 = 0.8265) and applicability. The modeling method developed in this study could be used to assess a regional secondary natural oak forest site index under complex site and climate variables to evaluate the forest productivity.

Soil quality assessment and its response to water flow connectivity in different vegetation restoration types, eastern China

Vegetation restoration is the most widely used forest management practice for degraded soils, but the responses of soil quality in different vegetation restoration types may vary. In addition, the relationship between water flow paths connection and soil quality needs to be further explored. In this study, the soil quality index (SQI) and the index of water flow connectivity (IWFC) in the six water flow patterns (PFP, SSB, WSB, CPS, CPW, and CSW) were explored at three forest stands (oak, pine, and bamboo forests) with 50 years for enclosure. The results showed that the bamboo forest stand had the best soil quality as a whole (0.534 ± 0.135), followed by pine (0.530 ± 0.180) and oak forest stands (0.435 ± 0.205). The IWFC in PFP, CPS and CPW water flow patterns decreased gradually with increasing soil depth, while in SSB, WSB and CSB water flow patterns, the IWFC increased at first and then decreased. Finally, the IWFC in the CPW water flow pattern showed the largest positive correlation with the SQI (P < 0.05). The IWFC in the CPW water flow pattern driven by soil physical properties could mainly control the changes in the SQI indirectly (average IE = 0.825) by influencing soil nutrient (average IE = 0.447) and biological (average IE = 0.485) properties, while its direct effects could be ignored (average DE = −0.074), which demonstrated that the joint effects of preferential flow paths and weak stream buffer zones can dramatically reflect the changes in soil quality. The better soil improvement effect of bamboo and pine forest stands compared to the oak forest stand should be given more consideration. This study provided new insights for the assessment of the relationship between soil hydrology and soil quality.

Robust Trend Analysis in Environmental Remote Sensing: A Case Study of Cork Oak Forest Decline

We introduce a novel methodological framework for robust trend analysis (RTA) using remote sensing data to enhance the accuracy and reliability of detecting significant environmental trends. Our approach sequentially integrates the Theil–Sen (TS) slope estimator, the Contextual Mann–Kendall (CMK) test, and the false discovery rate (FDR) control. This comprehensive method addresses common challenges in trend analysis, such as handling small, noisy datasets with outliers and issues related to spatial autocorrelation, cross-correlation, and multiple testing. We applied this RTA workflow to study tree cover trends in Los Alcornocales Natural Park (Southern Spain), Europe’s largest cork oak forest, analysing interannual changes in tree cover from 2000 to 2022 using Terra MODIS MOD44B data. Our results reveal that the TS estimator provides a robust measure of trend direction and magnitude, but its effectiveness is dramatically enhanced when combined with the CMK test. This combination highlights significant trends and effectively corrects for spatial autocorrelation and cross-correlation, ensuring that genuine environmental signals are distinguished from statistical noise. Unlike previous workflows, our approach incorporates the FDR control, which successfully filtered out 29.6% of false discoveries in the case study, resulting in a more stringent assessment of true environmental trends captured by multi-temporal remotely sensed data. In the case study, we found that approximately one-third of the area exhibits significant and statistically robust declines in tree cover, with these declines being geographically clustered. Importantly, these trends correspond with relevant changes in tree cover, emphasising the ability of RTA to detect relevant environmental changes. Overall, our findings underscore the crucial importance of combining these methods, as their synergy is essential for accurately identifying and confirming robust environmental trends. The proposed RTA framework has significant implications for environmental monitoring, modelling, and management.

The Effect of Spinosad on the Oak Lace Bug Corythucha arcuata (Hemiptera: Tingidae)-A Preliminary Study Performed Under Laboratory Conditions.

The effect of biopesticide compound spinosad in different concentrations was tested for the first time under laboratory conditions against the rapidly spreading forest pest, oak lace bug (Corythucha arcuata, Say 1832), and its effects were compared with the synthetic pesticide lambda-cyhalothrin. These results revealed a significant effect of spinosad at 2 mL/4 L and 2 mL/2 L water concentrations against C. arcuata nymphs. The mortality rate after 3 days was similar to synthetic insecticide effects and reached 94% and 98%, respectively. Overall, it can be concluded that spinosad is an effective biological method to control oak lace bug; treatments under field conditions should consider the high diversity of other insects in oak forests.

Differences in Soil CO2 Efflux and Microbial Community Composition among Slope Aspects in a Mountain Oak Forest

As one of the main terrain factors, the slope aspect generally shows remarkable effects on microclimate and vegetation distribution. The purpose of this study was to determine the potential effects of the slope aspect on soil respiration and the soil microbial community in a temperate mountain forest. A field investigation was carried out in a mountain oak forest located at different slope aspects (northeast, northwest, southeast, and southwest), and soil respiration was continuously measured for 12 months. The soil’s bacterial and fungal taxa were analyzed during the growing season. Our results showed that average soil respiration on the southwest and southeast slope aspects was 72.9% higher than the average on the northeast and northwest slope aspects. The coefficient of variation of soil respiration had the highest value on the northwest aspect (20.9%) and the lowest value on the southeast aspect (6.9%). The southeast slope had significantly higher soil respiration and temperature sensitivity compared to the other three slope aspects. The slope aspect substantially affected the soil’s bacterial and fungal r/K strategy, showing the lowest values on the northwest aspect and the highest values on the southeast aspect. Differences in bacterial r/K, the ratio of microbial biomass carbon (MBC) to soil organic carbon (SOC), and SOC among slope aspects contributed to a 43%, 38%, and 32% variation in soil respiration, respectively. The variation of soil temperature across slope aspects showed an indirect effect on soil respiration through changing bacterial r/K and MBC/SOC. Our findings highlight that terrain plays a critical role in regulating the spatial heterogeneity of soil respiration in mountain forests, which could be explained by the differences in SOC and microbial community composition across slope aspects.

Exploring the distribution pattern of native and alien forests and their woody species diversity in a small Mediterranean city

Recent studies have explored the ecological relationship between native urban forests and self-sown non-native forests in large cities and metropolises but further research efforts dedicated to analyzing this relationship in small cities are still needed. To improve our understanding of the ecology of urban native and alien forests in Mediterranean small cities, we analyzed the woody species richness, the community-weight mean of moisture and nitrogen ecological indicators, and soil disturbance indicators in the main urban wood types of the city of Campobasso (Italy), as well as their spatial distribution pattern across a gradient of cover and aggregation of green areas. The study showed that both native oak forests and Robinia pseudoacacia forests contributed to the maintenance of woody species richness. If we exclude the riparian environment, R. pseudoacacia forests occupied small marginal patches, tolerating soil disturbance and a high disturbance frequency, thus occupying habitats where the native oak forests could not grow. Conversely, R. pseudoacacia forests shared the ecological niche with the native riparian forests, which calls for action to prevent the spread of this alien species along river banks. Our results highlighted that urban remnant forests should be considered important assets for the planning and upkeep of urban green areas.

Application timing affects the efficacy of Bacillus thuringiensis subsp. kurstaki against Tortrix viridana in deciduous oak forests

The green oak leaf roller moth, Tortrix viridana, is one of the main defoliators of deciduous oaks in the Mediterranean region. Although aerial applications of Btk-based insecticides represent the most effective method to control the larval populations of this pest at a large spatial scale, the optimal time window for sprayings has not yet been defined. Accordingly, experimental trials were conducted in a Quercus pubescens forest district in Sardinia (Italy) involving both large-scale aerial treatments and small-scale applications from the ground. The aim of our work was to study how different larval development stages and/or sprouting phenological phases of the host trees affect Btk efficacy. Aerial sprayings were conducted in 2003 and 2004 in two different areas of about 300 ha, selected according to altitude and exposure to simulate three different application timings against different larval instars and sprouting phenological phases. From-the-ground applications were carried out in 2021 in a Q. pubescens forest stand by applying Btk-based insecticides at different timings, and the overall Q. pubescens phenological stage and the distribution of T. viridana larval development stages were assessed accordingly. The effectiveness of Btk applications was evaluated by estimating larval mortality 7, and 14 days after aerial applications, and 7, 14 and 21 days after ground applications. Defoliation due to T. viridana was also evaluated at the end of larval development in all years. In both 2003 and 2004, the average larval mortality due to Btk exceeded 80 % two weeks after aerial applications across all application timings. Damage caused by T. viridana varied with Btk application timing, with the later applications showing a higher defoliation than other timings. In ground application experiments, larval mortality significantly increased when Btk was sprayed against a larval population primarily composed of IV instars, which were feeding on sprouts with leaves that were spread out and had not fully developed internodes, thus resulting in a significative reduction of insect damage. Our findings indicate that, in accordance with the strict synchrony between the larval development and the oak flushing, the optimal timing for Btk application against T. viridana can be defined based on the bud burst phenological phase. In particular, the prevalence of sprout with expanding leaf surface allowing insecticidal droplet interception and the simultaneous presence of susceptible larval instars (within the IV), resulted in an increased treatment efficacy.

Climate change and exotic pathogens shift carbon allocation in Mediterranean mixed forests

Abstract Forecasting the effects of global change drivers on ecosystems is one of the most pressing challenges for scientists worldwide. Particularly, climate change and exotic pathogens might have a large impact on plant community dynamics and ecosystem functioning through changes in carbon uptake and sinks. Nevertheless, we still have a poor understanding of the combined effects of these two drivers on plant communities. Here, we explored the impact of rainfall reduction and exotic pathogens on the carbon balance of Mediterranean tree species. For this, we performed a 3‐year field experiment taking advantage of rainfall exclusion infrastructures (30% exclusion) installed in the southernmost European oak forests invaded by the aggressive exotic pathogen Phytophthora cinnamomi. We measured a set of 10 variables representative of tree carbon sources (photosynthetic rates) and sinks (primary production, reproduction, defence, and reserves) in adult trees of three species in two forest types: closed forests of Quercus suber and Q. canariensis, and open woodlands of Q. suber and Olea europaea. We found a large variability in the sensitivity of the different carbon sources and sinks to the effects of drought and pathogens, from variables highly sensitive to both factors (carbon fixation and reproduction, root chemistry) to variables only responsive to drought (litter production) or totally unresponsive (tree trunk, leaf chemistry). Although negative effects predominated, positive effects of rainfall exclusion were also detected in wet years, likely due to a reduction of pathogen abundance in drier soil. Trade‐offs between carbon sinks appeared in all tree species, but rainfall exclusion only modified trade‐offs in Q. suber, the species most susceptible to P. cinnamomi. Synthesis. We provide evidence on the complexity of the combined effects of abiotic (drought) and biotic (pathogens) global change drivers on carbon source and sinks of adult trees, including both negative direct effects and positive indirect effects. Our results showed that these effects varied among co‐existing species, particularly for carbon sinks directly related to tree demography (reproduction). Therefore, long‐term changes in the structure of Mediterranean mixed forests might be expected towards the dominance of species highly resistant to both drought and pathogens.

Latitudinal gradients of biodiversity and ecosystem services in protected and non-protected oak forest areas can inform climate smart conservation

Adaptive governance of areas set aside for future protection of biodiversity, sustainable production, and recreation requires knowledge about whether and how effects of area protection are modulated by climate change and redistribution of species. To investigate this, we compare biodiversity of plants (assessed using vegetation plots) and arthropods (collected with Malaise traps, analyzed using metabarcoding) and productivity (tree growth, determined using dendrochronology) in protected and non-protected oak (Quercus spp.) forests along a latitudinal gradient (55.6 °N – 60.8 °N) in Sweden. We also compare historical, recent and projected future climate in the region. In contrast to established global latitudinal diversity gradients, species richness of plants and arthropods increased northwards, possibly reflecting recent climate-induced community redistributions, but neither was higher in protected than in non-protected areas, nor associated with contemporary ground temperature. Species composition of arthropods also did not differ between protected and non-protected areas. Arthropod biomass increased with latitude, suggesting that the magnitude of cascading effects mediated via their roles as pollinators, herbivores, and prey for other trophic levels, varies geographically and will change with a moving climate. Annual growth rate of oaks (an ecosystem service in the form of biomass increase and carbon sequestration) was independent of latitude and did not differ between protected and non-protected areas. Our findings question the efficacy of contemporary designation and management of protected oak forests, and emphasize that development and implementation of modified climate smart conservation strategies is needed to safeguard ecosystem functioning, biodiversity, and recreational values of protected forest areas against future challenges.

Soil moisture and water redistribution patterns in white oak (Quercus alba) saplings and trees in fragmented urban woodlands

In the midwestern United States, models predict extended summer heatwaves and increasingly frequent and prolonged drought conditions. In the Chicago region, the potential for large-scale mortality of white oak trees (Quercus alba) coupled with the ongoing decline of white oak sapling recruitment are major concerns for researchers and practitioners. In this study, we determined the sources of water used by mature white oak trees and saplings in three qualitatively different sites within a remnant oak forest in Chicago during the 2021 drought. We investigated soil moisture dynamics (volumetric water content, VWC) and water isotope composition of leaf tissues (δD, δ18O), rainwater, and groundwater. These data were linked to sapling height (proxy for biomass) and ectomycorrhizal (ECM) functional types. We predicted that: (i) mature oak trees use deeper water sources and conducted hydraulic redistribution (HR), and (ii) mature trees shared water with saplings during dry periods via long-distance ECM functional types. Soil moisture decreased progressively from June to October (spring to fall), with August and September having the lowest moisture (<20 % VWC). Following rainfall recharge, temporal patterns of soil moisture showed gravity drainage and then ongoing stair-stepwise drawdown consistent with plant evapotranspiration. Leaf δD and δ18O values in mature trees and saplings were consistent with water uptake from rainfall and subsequent enrichment via evapotranspiration. In two sites, mature trees and saplings demonstrated distinct δD: δ18O slopes, with mature trees more enriched than saplings. In the third site, mature trees and saplings δD: δ18O slopes overlapped but here, the ECM community was dominated by contact-type ECM and sapling height increased with distance from the mature oak. Our findings indicate that HR was not a component of site ecohydrology, and future climate conditions may present increasing challenges for white oak recruitment as both mature trees and saplings compete for limited rainfall-derived soil moisture.

Applying fluorescence and 2D-correlation spectroscopy to elucidate changes in the chemical composition of DOM from soils with different vegetation types

ABSTRACT To understand the decomposition of the dissolved organic matter (DOM), four different soils (from grassland, spruce forest, oak forest, and agricultural land) were incubated at 15 °C, and the DOM changes were monitored using UV-Vis, synchronized fluorescence and 2D-correlation spectroscopy. The DOM fraction of the agricultural and grassland soils showed a significant increase in both SUVA values in the middle of the incubation ranging from an initial 3–5 L mg−1 m−1 to 40–50 L mg−1 m−1. Synchronous fluorescence spectroscopy revealed that at the beginning of incubation, on day 3, there was an increase in the proportion of low molecular-weight, readily biodegradable compounds (PLF, protein-like fluorescence), while from day 8 onwards, microbial degradation generated a large number of medium-molecular-weight molecules (MHLF, microbial humus-like fluorescence). At further sampling dates, the proportion of compounds of microbial origin decreased systematically, due to a decrease in microbial activity caused by the lack of substrate. 2D-correlation measurements revealed further details of the changes in the quality of DOM, revealing two distinct patterns in the DOM dynamics of the soils, the sequence MHLF→PLF being observed for grassland and arable land and PLF→HLF for forest soils, probably due to the different N supplies of the soils.

Response of parasitoid communities to insecticide application during a Lymantria dispar outbreak in mixed oak forests

Abstract In the temperate mixed oak forests of Central Europe, outbreaks of insects such as the spongy moth, Lymantria dispar, can cause severe defoliation and insecticide is sometimes applied for their control. Parasitoids, mainly Hymenoptera and Diptera, are among the most diverse and important natural enemies of caterpillars in these forests. However, due to their cryptic lifestyle and taxonomic difficulties, we lack knowledge on the impact of insecticide applications on complex host‐parasitoid networks. In a large‐scale field experiment, we tested the effect of spraying the lepidopteran‐specific insecticide Mimic (tebufenozide) on the abundance and community composition of both adult and larval parasitoids. We combined morphological identification, DNA barcoding and metabarcoding to identify parasitoids adult or inside caterpillars, both sampled by canopy fogging during an outbreak and two subsequent years. We analysed the abundance of parasitoids, community composition and network specialisation using statistical methods that account for sample incompleteness in host‐parasitoid data. For adult parasitoid assemblages, we found strong annual effects on abundance, with highest numbers of adult parasitoids occurring in the outbreak year, as well as on annual changes in community composition, but no effect linked to insecticide application. However, the abundance and species number of immature parasitoids revealed negative effects of insecticide application, while community composition was only affected by annual variation. Coverage‐based network analyses showed a reduction of taxonomic network diversity and network specialisation associated with insecticide application in the first 2 years. Synthesis and applications: This real‐world experiment shows that parasitoid populations respond immediately to large‐scale outbreaks but only limited to local disturbances. Results indicate that this group of natural enemies exhibits high mobility, enabling them to track host populations across large spatial scales. However, our observation of reduced network specialisation after insecticide application is a warning signal that ecosystem function, and consequently natural pest control services, may be impaired by human interference at the local stand scale.

Contribution to the Iberian thermomediterranean oak woods (Spain, Portugal): the importance of their teaching for the training of experts in environmental management

The study of the holm oak forests of the lower and upper mesomediterranean of the south of the Iberian Peninsula, and new syntaxons of holm oak forests not assigned until now are contributed. The plant dynamics is provided and completed, and the importance of the learning of plant description and dynamics by university students, future environmental managers, is specified. Taking into account the strong anthropic action with the consequent destruction of holm oak forests, it is necessary to enhance the knowledge of plant dynamics to decipher the potential vegetation, and to be able to apply the vegetation series as a basis for agronomic and forestry management. In this work, we propose a new vegetation series. We propose the association Aristolochio baeticae-Quercetum rotundifoliae nova for the biogeographic sector Granadino-Almijarense (Montes de Málaga) and as a first dynamic stage of the microforest of Olea europea var. sylvestris obtained by deforestation. The study of the Iberian olive groves allows us to propose two new associations Aristolochio baeticae-Oleetum sylvestris and Ephedro fragilis-Oleetum sylvestris.

Isolation and Characterization of Compounds from Truffle Melanogaster broomenaus and their Bioactivities.

Melanogaster broomeanus Berk. is a type of truffle common in pine, oak, and hazelnut forests and which naturally spreads in the Black Sea, Mediterranean, South-West Anatolia in Turkey, is consumed as food and traded. Chemical investigation of M. broomenaus led to isolate of ten known compounds namely, brassicasterol (1), ergosterol (2), ergosterol peroxide (3), 5α, 6α-epoxy ergosta-7,22-diene-3β-ol (4), trametenolic acid (5), maleic acid (6), fumaric acid (7), mannitol (8), kojibiose (9) and trehalose (10). Assessments of the antioxidant, anticholinesterase, anti-urease, and cytotoxic properties of extracts and isolated compounds were carried out. Compound 5 showed the highest cytotoxic activity against H-1299 with IC50: 23.78±0.98 μg/mL, while compound 2 demonstrated most potent cytotoxicity against MCF-7 with IC50: 30.91±0.15 μg/ml. Also, compound 5 showed the highest antioxidant activity in all methods, particularly in DPPH and ABTS assays with IC50 values of 90.24±0.87, 75.41±0.10 μg/mL, respectively. Similarly, compound 5 exhibited highest activity with 38.84±1.22 % and 44.58±0.96 % inhibitions against AChE and BChE, respectively, while compound 7 were found to be most potent inhibitory against urease with value of 55.20±0.45 % at 100 μg/mL concentration. These findings suggest that M. broomenaus, like numerous other edible mushrooms, is a steroid-rich truffle and a potential source of steroids.

Multitemporal Analysis of Land Cover Changes in Areas with Contrasted Forest Management and Conservation Policies in Northern Mexico

This study evaluates and contrasts changes in vegetation cover over three decades in two forest areas in the State of Chihuahua in northern Mexico with different management statuses: one with sustainable forest management and the other protected as a Flora and Fauna Protection Area. The hypothesis proposed that both areas would have maintained or increased their vegetation cover since 1995. Satellite images from the periods 1995–2008, 2008–2014, 2014–2022, and 1995–2022 were analyzed. The results showed that Ejido El Largo y Anexos significantly increased forest areas and reduced grasslands due to sustainable management practices, with a notable expansion of pine–oak and pine forests. In contrast, the Tutuaca Flora and Fauna Protection Area experienced a notable loss of oak and oak–pine forests, suggesting ineffectiveness in its conservation policies. However, there was less loss in Douglas Fir forests, indicating some effective protection efforts. The comparison reveals opposing dynamics: while Ejido El Largo y Anexos demonstrates success in sustainable management, the Tutuaca Flora and Fauna Protection Area faces conservation challenges. In conclusion, this study highlights the need for active management approaches to maintain ecosystem cover and functionality.