SignificanceThe concurrent use of double photosensitizers with multiple irradiation sessions is emerging as a promising modality for photodynamic therapy. ApproachCandida albicans (ATCC10231) biofilm was treated with either erythrosine 220 μM, 100 mM KI, or 220 μM erythrosine+100 mM KI. Control groups were phosphate buffer saline, 1:100,000 U/ml nystatin solution, and Light-only respectively. After 60-secs incubation, experimental groups were irradiated with LED (520-540 nm, 250 mW/cm2, 20 J/cm2/session) for 1 or 3 sessions. The logarithmic transformation of biofilm count was calculated and a comparison at 1, 24 hrs was performed using median±interquartile range. The statistically significant difference was determined using Kruskal Wallis test with post hoc test at p<0.05 Results220 μM Erythrosine+100 mM KI with 60 J/cm2 (20 J/cm2x3 sessions) exerted the most anticandidal effect that comparable to nystatin effect. ConclusionsErythrosine 200 μM+KI 100 mM+3 sessions green LED (total energy 60 J/cm2) effectively reduced C. albicans biofilm.