Nutritional Requirements Research Articles

Nutraceuticals: New Perspective and Approach to Prevent Dysmenorrhea

Background: The words "nutrition" and "pharmaceutical" are combined to form the phrase "nutraceutical." Nutraceuticals are foods or dietary components that have an important role in regulating and sustaining normal physiological function in humans. For the treatment of dysmenorrhoea, a variety of pharmacological medications are available, however, they all have significant side effects. Dietary supplements and lifestyle management, on the other hand, were found to have a major influence on the occurrence and control of dysmenorrhea. Objective: The objective of this paper is to study a comprehensive review of the dietary and nutritional supplements with special emphasis on dysmenorrhea. This paper focuses on understanding and interpreting the details of menstrual pain and its effective nutritional diet that can be used as a treatment for avoiding menstrual discomfort. Methods: The selection of data has been done by studying a combination of various research and review papers from different databases like PubMed, NCBI, Science Direct, WHO, Cochrane Library, and Web of Science from the year 2000-2022. Results: Based on various papers and literature reviews, we have concluded about various dietary supplements and herbs that can be used to avoid pain during mensuration followed by its all-daily requirement of nutrition on different age groups and efficacy of treatments. Conclusion: According to current evidence, young women should be instructed in the proper use of dietary supplements, nutrition, and vitamins, as well as the inclusion of effective diet and lifestyle changes such as exercise and a well-balanced diet with adequate nutrition, as these are likely to reduce the negative effects of dysmenorrhea.

Proactive cursorial and ambush predation risk avoidance in four African herbivore species.

Most herbivores must balance demands to meet nutritional requirements, maintain stable thermoregulation and avoid predation. Species-specific predator and prey characteristics determine the ability of prey to avoid predation and the ability of predators to maximize hunting success. Using GPS collar data from African wild dogs, lions, impala, tsessebes, wildebeest and zebra in the Okavango Delta, Botswana, we studied proactive predation risk avoidance by herbivores. We considered predator activity level in relation to prey movement, predator and prey habitat selection, and preferential use of areas by prey. We compared herbivore behaviour to lion and wild dog activity patterns and determined the effect of seasonal resource availability and prey body mass on anti-predator behaviour. Herbivore movement patterns were more strongly correlated with lion than wild dog activity. Habitat selection by predators was not activity level dependent and, while prey and predators differed to some extent in their habitat selection, there were also overlaps, probably caused by predators seeking habitats with high prey abundance. Areas favoured by lions were used by herbivores more when lions were less active, whereas wild dog activity level was not correlated with prey use. Prey body mass was not a strong predictor of the strength of proactive predation avoidance behaviour. Herbivores showed stronger anti-predator behaviours during the rainy season when resources were abundant. Reducing movement when top predators are most active and avoiding areas with a high likelihood of predator use during the same periods appear to be common strategies to minimize predation risk. Such valuable insights into predator-prey dynamics are only possible when using similar data from multiple sympatric species of predator and prey, an approach that should become more prevalent given the ongoing integration of technological methods into ecological studies.

Influence of supplemental choline on milk yield, fatty acid profile, and weight changes in postpartum ewes and their offspring.

The most intensive nutritional requirements occur during milk production's peak. Ewe milk contains more protein and fat than cow milk. The nutritional factors significantly determine the composition. The liver undergoes high stress during lactation but is relieved by essential nutrients. Choline acts metabolically as a lipotrope. This compound functions in cell structure construction, maintenance, and acetylcholine synthesis. The animal nutrition industry provides choline from various sources, such as synthetic and natural kinds. This study evaluated the influence of two distinct choline sources on dairy ewes' peripartum and postpartum milk production, composition, and offspring growth. Twenty-four Rambouillet ewes, each weighing around 63.7 ± 1.7 kg, aged three with two previous births, spent 30-day pre-partum and post-partum in individual pens (2 × 2 m). They were given different experimental treatments 30 days before and after birth according to a randomized design; no choline (a), 4 g/day rumen-protected choline (RPC) (b), or 4 g/day thiocholine (c). Milk samples for milk composition and long-chain fatty acid (FA) analysis were taken every 30 days during milk collection. Significant differences (p < 0.05) in ewe body weight, lamb birth weight, and 30-day-old lamb body weight were observed at lambing and on day 30 of lactation due to choline treatment. Milk yield was significantly higher (1.57 kg/day) compared to the control (1.02 kg/day) and RPC (1.39 kg/day), due to the herbal choline source. There was no significant difference in the milk's protein, lactose, fat, non-fat solids, and total milk solids content between the treatments. Herbal choline lowers (p < 0.05) the concentrations of caproic, caprylic, capric, lauric, and myristic acids while boosting (p < 0.05) those of oleic and cis-11-eicosenoic acid, the changes influencing long-chain FA levels (p < 0.05). Providing choline from both sources to ewes enhanced milk production and body weight at lambing and on 30-day post-lambing. The herbal choline supplement altered short-chain milk FAs, while representative concentration pathways affected medium-chain ones.

Scoping Review of Home Parenteral Nutrition Requirements in Adults with Chronic Intestinal Failure

Scoping Review of Home Parenteral Nutrition Requirements in Adults with Chronic Intestinal Failure

Deciphering nutritional stress responses via knowledge-enriched transcriptomics for microbial engineering

Understanding diverse bacterial nutritional requirements and responses is foundational in microbial research and biotechnology. In this study, we employed knowledge-enriched transcriptomic analytics to decipher complex stress responses of Vibrio natriegens to supplied nutrients, aiming to enhance microbial engineering efforts. We computed 64 independently modulated gene sets that comprise a quantitative basis for transcriptome dynamics across a comprehensive transcriptomics dataset containing a broad array of nutrient conditions. Our approach led to the i) identification of novel transporter systems for diverse substrates, ii) a detailed understanding of how trace elements affect metabolism and growth, and iii) extensive characterization of nutrient-induced stress responses, including osmotic stress, low glycolytic flux, proteostasis, and altered protein expression. By clarifying the relationship between the acetate-associated regulon and glycolytic flux status of various nutrients, we have showcased its vital role in directing optimal carbon source selection. Our findings offer deep insights into the transcriptional landscape of bacterial nutrition and underscore its significance in tailoring strain engineering strategies, thereby facilitating the development of more efficient and robust microbial systems for biotechnological applications.

Granulicatella adiacens: An uncommon pathogen in prosthetic joint infections

Granulicatella adiacens is an uncommon culprit in prosthetic joint infections, especially when predisposing factors are absent. Here, we present the case of a 78-year-old female who developed an acute surgical site infection shortly after undergoing left total knee arthroplasty. Despite initial negative cultures, subsequent analysis identified Granulicatella adiacens in the knee fluid, marking only the second reported instance in the United States and the seventh globally. This singular case highlights the considerable challenges associated with isolating this organism, emphasizing its atypical presentation and diagnostic elusiveness. Typically associated with endocarditis and bacteremia, its involvement in isolated surgical site infections is particularly unusual. Our case underscores the necessity for heightened clinical suspicion and the adoption of specialized diagnostic methodologies to accurately detect Granulicatella adiacens infections. Given its fastidious nature and nutritional requirements, standard culture methods may fail to identify this pathogen, necessitating alternative diagnostic approaches. As such, the utilization of specialized media becomes paramount in clinical diagnostics to facilitate appropriate management and optimize healthcare resource allocation. By enhancing our ability to detect and treat infections caused by Granulicatella adiacens, we can mitigate patient morbidity and reduce the burden of unnecessary interventions, thereby improving patient outcomes and healthcare efficiency.

Nutrition Requirements and Nutrition Intervention for People Living with HIV/AIDS (Adults)

AbstractNutrition plays a crucial role in the overall health and well-being of people living with human immunodeficiency virus (HIV). This review aims to provide a current summary of the macronutrient and micronutrient requirements for HIV-positive individuals. The author emphasizes the fundamentals of nutrition treatment as a guide for medical professionals to adopt the right strategy based on risk-adapted nutrition therapy. The majority of nutrition guidelines were published over 10 years ago and have failed to address the protein requirements for people with human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS). Proper nutrition can reduce the risk of malnutrition, infections, and other complications, while also assisting with symptom management, maintaining a healthy weight, and improving immune function. In this review, the author has identified the latest reliable and validated nutrition screening tool specifically designed for people with HIV/AIDS, along with a risk-stratified nutrition therapy based on the screening tool to facilitate the nutrition care process. People living with HIV/AIDS have unique nutritional needs due to the virus's impact on the body's immune system, metabolism, and nutrient absorption. It is important for them to work with a healthcare professional, namely a dietitian, to assess nutrition risk and develop an individualized dietary intervention for a healthy lifestyle.

Assessment of the nutritional quality of weaning food prepared from fermented blends of sorghum (Ogi) and soybean

This study investigated the nutritional quality of weaning food prepared from fermented blends of sorghum (ogi) and soybean. The raw samples were processed into flour and were mixed in ratio of 60:40 and 70:30 respectively. Lactic acid bacteria (LAB) were isolated from fermented ogi and were accessed for their technological properties. The pH and total titratable acidity of the ogi was accessed, proximate composition and shelf-life of the weaning blends were determined by standard methods. A total of 10 LAB isolates were obtained, characterized and identified as L. plantarum (50%), L. fermentum (30%), L. delbrueckii (10%), and L. brevi (10%). Lactic acid, diacetyl and hydrogen peroxide produced by the LAB isolates ranged from 1.203 ± 0.006 - 3.410 ± 0.010, 1.21 ± 0.02 - 3.63 ± 0.05 and 0.650 ± 0.060 - 1.910 ± 0.040 (mg/mL) respectively. The fermentation of the ogi resulted in pH reduction from 4.5 ± 0.01- 2.40 ± 0.01 and increased titratable acidity 12.49 ± 0.01 - 23.00±0.01 g/L. The 60:40 weaning blend of sorghum ogi flour and soybean improved protein, ash, crude fiber, moisture, fat, vitamin A, ascorbic acid, riboflavin, and beta carotene content compared to a 70:30 blend. The blend also showed improvements in mineral content, with a significantly higher overall nutritional value compared to the 70:30 blend. Coliforms were not observed during the shelf-life monitoring. This study concluded that these blends meet daily nutritional requirements, provide health benefits, and meet recommended standards for weaning.

Contribution of livestock to organic agriculture: Modelling nitrogen flows at the national scale

Organic agriculture (OA) is the promoted sustainable agriculture model in the European Union (EU), yet its expansion is hindered by limited nutrient availability, particularly nitrogen (N). OA's main sources of N include biological N fixation by legume crops and manure from both conventional and organic livestock. However, potential stricter EU regulations on allowed external N resources for OA and pressure to reduce livestock numbers could impact N availability in OA. Understanding national-scale N flows is essential. Here, we analysed N flows in organic agri-food systems in France, the largest OA area in Europe. We show that approximately 20 % of the manure used to fertilise organic cropland came from conventional agriculture and 15 % from the dietary N nutritional requirements of organic livestock imported from outside France. N surplus is half that of the conventional agriculture at national scale. This first national assessment highlights biophysical and regulatory constraints providing insights into the possibilities of achieving the EU's target of having 25 % of agricultural land under OA.

Разработка хлебобулочных изделий нутриентно-адаптированных для питания спортсменов-легкоатлетов

Proper nutrition of an athlete is an important component of the effectiveness of sports results. An athlete’s daily diet should cover his increased nutrient needs due to heavy physical activity. The purpose of the research was to develop bakery products that were nutrient-adapted to the nutritional requirements of athletes using the example of track and field athletes. The recipe for the new product included wheat flour, oat flour, micellar casein, flaxseed oil, date syrup, and crushed walnut kernels. A study of the influence of new ingredients on the quality of semi-finished products and finished products showed that the addition of oat flour and casein slightly increased the moisture and acidity of the finished products, but the indicators remained within the limits defined by the regulatory documentation for bakery products made from wheat flour. With an increase in the dosage of oatmeal and casein, the products acquired a slight taste of oatmeal; casein had no effect on taste. A new type of bakery product is characterized by good organoleptic and physicochemical quality indicators and has increased nutritional value. Consumption of 100 g of the developed “Fitbulka” bakery product allows you to satisfy the daily need for biologically complete protein by 8.9%, carbohydrates by 5.3%, fat by 7.2%, including polyunsaturated fatty acids of the omega-3 family by 39.6% and omega-6 by 5.4%. The degree of satisfaction of the daily requirement for minerals increases in comparison with the control sample and amounts to 16.0% for calcium, 13.5% for phosphorus, 17.4% for magnesium, 7.4% for potassium, and 7.5% for iron. Analysis of amino acid score calculations showed that the biological value of the protein fraction of the test sample was 17.6% higher compared to the control. The developed “Fitbulka” bakery product can be recommended for inclusion in the diet of athletes as an additional source of polyunsaturated fatty acids, dietary fiber, protein, calcium and magnesium in the diet.

Potential of cassava peel collected from Bauchi (Nigeria) as culture media for growing specific fungi

The objective of this paper is to assess the potential of using cassava peel for A. niger and penicillium growth using standard methods and chemicals of analytical grade. The result of the study indicates that, the peel is rich in proximate contents as the analyzed cassava peel is composed of 8.70% moisture, 4.89% crude protein, 6.9.00% carbohydrates, 4.00 % fat content, 8.93% ash, and 8.75% fibre. Considering the observed morphological and physical characteristics of the test fungi; therewith, A. niger, and penicillium were present. There is indication that, the two utilized media (control and formulated media) are in support of the two microbes growth and depicted positive results of degradation, as well as fermentation ability on the cassava peels. The enriched cassava agar supports higher growth of the two analyzed microbes (viz, A. niger, and penicillium). There was significant difference pertaining the colonies number made on the formulated media (cassava peel agar, and enriched cassava agar). Potato dextrose agar (PDA) shows lower colony count amounting to 3.4 0.1 CFU/ml for A. niger, and amounting to 3.3 0.2 CFU/ml for penicillium. Cassava peel agar (CPA) possessed 6.5 0.5 CFU/ml mean colony count (MCC) for A. niger, and 5.6 0.1 CFU/ml for penicillium. Enriched cassava agar media (ECA) had highest MCC as 7.8 0.1 CFU/ml with respect to A. niger, and 7.3 0.6 CFU/ml pertaining penicillium. Utilization of cassava peel as substrate due to its contents of nutrients that can meet the nutritional requirement of fungi growth is a better move. The peel may be considered as substitute and alternative substrate for producing culture media to cultivate fungi and consequently reducing pollution.

Exploring Phosphate Solubilizing Bacterial Communities in Rhizospheres of Native and Exotic Forage Grasses in Alkaline-Sodic Soils of the Flooding Pampa.

The flooding pampa is one of the most important cattle-raising regions in Argentina. In this region, natural pastures are dominated by low-productivity native grass species, which are the main feed for livestock. In this context, previous studies in the region with the subtropical exotic grass Panicum coloratum highlight it as a promising species to improve pasture productivity. Cultivable phosphate solubilizing bacteria (PSB) communities associated to native (Sporobolus indicus) and exotic (Panicum coloratum) forage grasses adapted to alkaline-sodic soils of the flooding pampa were analyzed. PSB represented 2-14% of cultivable rhizobacteria and Box-PCR fingerprinting revealed a high genetic diversity in both rhizospheres. Taxonomic identification by MALDI-TOF showed that PSB populations of P. coloratum and S. indicus rhizospheres are dominated by the phylum Proteobacteria (92,51% and 96,60% respectively) and to a lesser extent (< 10%), by the phyla Actinobacteria and Firmicutes. At the genus level, both PSB populations were dominated by Enterobacter and Pseudomonas. Siderophore production, nitrogen fixation, and indoleacetic acid production were detected in a variety of PSB genera of both plant species. A higher proportion of siderophore and IAA producers were associated to P. coloratum than S. indicus, probably reflecting a greater dependence of the exotic species on rhizospheric microorganisms to satisfy its nutritional requirements in the soils of the flooding pampa. This work provides a novel knowledge about functional groups of bacteria associated to plants given that there are no previous reports dedicated to the characterization of PSB rhizosphere communities of S indicus and P coloratum. Finally, it should be noted that the collection obtained in this study can be useful for the development of bioinputs that allow reducing the use of chemical fertilizers, providing sustainability to pasture production systems for livestock.

Temporal variation in nutritive value of forage in kermes oak shrublands

This study was carried out to determine the seasonal floristic composition of grasses, forbs, and shrubs in a kermes oak (Quercus coccifera L.) shrubland in Chalkidiki, Greece, to estimate their nutritive value and to relate it to the nutritional requirements of small ruminants. All the herbaceous and woody species that were present in the study area were identified and collected at the beginning of each season. The samples were categorized into grasses, forbs, and shrubs. Dry matter (DM), ash, ether extract (EE), neutral detergent fiber assayed with heat stable amylase (aNDF), crude protein (CP), in vitro dry matter digestibility (IVDMD), calcium (Ca), and phosphorus (P) contents were determined. Forty-three plant taxa were identified (30 forbs, 7 grasses, and 6 shrubs). Forbs had higher mean values of ash (94.7 g/kg DM), CP (120.9 g/kg DM), Ca (16.9 g/kg DM), P (1.2 g/kg DM), and IVDMD (590.5 g/kg DM) compared to grasses (72.5 g/kg DM, 86.5 g/kg DM, 3.9 g/kg DM, 0.9 kg-1 DM, and 517.8 g/kg DM), and shrubs (44.5 g/kg DM, 79.9 g/kg DM, 11.0 g/kg DM, 0.7 g/kg DM, and 494.7 g/kg DM), respectively. Cool-season grasses can offer sufficient nutrients to grazing animals during autumn and winter, while shrubs support them during spring and winter.

Need for operational simplicity and timely disbursal of benefits—a qualitative exploration of the implementation of a direct benefit transfer scheme for persons with tuberculosis in India

BackgroundNi-kshay Poshan Yojana (NPY) is a direct benefit transfer scheme of the Government of India introduced in 2018 to support the additional nutritional requirements of persons with TB (PwTB). Our recent nationwide evaluation of implementation and utilization of NPY using programmatic data of PwTB from nine randomly selected Indian states, reported a 70% coverage and high median delay in benefit credit. We undertook a qualitative study between January and July 2023, to understand the detailed implementation process of NPY and explore the enablers and barriers to effective implementation and utilization of the NPY scheme.MethodsWe followed a grounded theory approach to inductively develop theoretical explanations for social phenomena through data generated from multiple sources. We conducted 36 in-depth interviews of national, district and field-level staff of the National Tuberculosis Elimination Programme (NTEP) and NPY beneficiaries from 30 districts across nine states of India, selected using theoretical sampling. An analytical framework developed through inductive coding of a set of six interviews, guided the coding of the subsequent interviews. Categories and themes emerged through constant comparison and the data collection continued until theoretical saturation.ResultsStakeholders perceived NPY as a beneficial initiative. Strong political commitment from the state administration, mainstreaming of NTEP work with the district public healthcare delivery system, availability of good geographic and internet connectivity and state-specific grievance redressal mechanisms and innovations were identified as enablers of implementation. However, the complex, multi-level benefit approval process, difficulties in accessing banking services, perceived inadequacy of benefits and overworked human resources in the NTEP were identified as barriers to implementation and utilization.ConclusionThe optimal utilization of NPY is enabled by strong political commitment and challenged by its lengthy implementation process and delayed disbursal of benefits. We recommend greater operational simplicity in NPY implementation, integrating NTEP activities with the public health system to reduce the burden on the program staff, and revising the benefit amount more equitably.

Feed balances for ruminant livestock: gridded estimates for data−constrained regions

Demand for animal−source foods and livestock feed are forecast to increase across sub-Saharan Africa. In this context, there is a need to estimate the availability of livestock feed to support decision−making at local, sub-national and national levels. In this study, we assess feed balances for ruminant livestock in Ethiopia and Burkina Faso. Feed availability was estimated using remotely sensed products and detailed feed composition data. Feed requirements were estimated for maintenance, growth, lactation, gestation and locomotion using a data−intensive model. Biomass available as animal feed was estimated to be 8.6 tonnes of DM per hectare in the Ethiopian highlands and midlands, 3.2 tonnes DM per hectare in the Ethiopian lowlands, 2.9 tonnes DM per hectare in Burkina Faso’s Sudanian agro-ecological zone and 1.0 tonne DM per hectare in the Sahel. The energy requirements of lactating cows were estimated to be 62.1 Megajoules (MJs) per animal per day in the Ethiopian highlands and midlands, 62.7 MJ in the Ethiopian lowlands, 88.5 MJ in Burkina Faso’s Sudanian agro-ecological zone and 53.1 MJ per animal per day in the Sahel. Feed scarcity hotspots are most prominently located in the Ethiopian highlands and the Sahelian agro-ecological zone of Burkina Faso. Demand−side policy and investment initiatives can address hotspots by influencing herd sizes, nutritional requirements and herd mobility. Supply−side policy and investment initiatives can secure existing feed resources, develop new sources of feed and incentivise trade in feed resources. Improving feed balances will be of value to decision−makers with the aims of optimising livestock productivity, minimising exposure to climatic shocks and minimising greenhouse gas emission intensity.

Enhancing potassium assimilation in soil for Zingiber officinale plant by rhizosphere bacterial isolate and optimization studies by response surface modeling

Rhizosphere bacteria have always proven their worth towards their associated plant in their defense mechanism and nutritional functions. This study was made to understand the impact of rhizosphere-associated bacterial isolate PGK01 in complimenting associated Zingiber officinale plants in their potassium nutritional requirements. The screening of the isolate suggested that it can solubilize potassium, which the plant shall use for its nutritional purposes. Molecular sequencing suggested that the strain belonged to Bacillus subtilis, whose sequence has been submitted to NCBI under the accession number OQ652138. In-situ analysis of the effect of bacteria on the growth parameters and yield of the plant indicated that in the presence of microbe, plants tend to grow more when compared to without the organism. The optimization studies conducted by response surface modeling suggested that only half of the potassium fertilizer is necessary when a plant is grown in the presence of isolate compared to the conventional method. The adequacy of the statistical model was verified by analyzing the Sequential Model Sum of Squares and Cook’s distance diagnostic, wherein the observation proved the significance of the mathematical model.

Malnutrition in sickle cell anemia: Prevalence, impact, and interventions: A Review.

Sickle Cell Anemia (SCA) is a hereditary hemoglobinopathy characterized by chronic hemolytic anemia, vaso-occlusive events, and a wide range of clinical complications. Malnutrition, often an underexplored aspect of this complex condition, plays a critical role in disease management and overall patient well-being. This publication provides a comprehensive review of the prevalence, impact, and interventions related to malnutrition in individuals with SCA. A thorough literature review reveals the multifaceted challenges faced by SCA patients in maintaining adequate nutrition. The pathophysiology of SCA, involving chronic inflammation, oxidative stress, and hypermetabolism, contributes to increased nutritional requirements and altered dietary patterns. Factors such as reduced appetite, nutrient malabsorption, dietary restrictions, and socioeconomic disparities further exacerbate the risk of malnutrition. Malnutrition is a prevalent issue among individuals with SCA, affecting patients of different age groups and disease severities. Nutritional deficiencies, including vitamins, minerals, and essential nutrients, are common in this population. The impact of malnutrition on disease outcomes is significant, with associations between nutrient status and complications such as pain crises, infections, and impaired quality of life. This paper also reviews nutritional interventions aimed at addressing malnutrition in SCA patients. While dietary counseling, supplementation, and personalized nutrition plans have shown promise in improving nutritional status, challenges such as patient adherence and access to healthcare must be addressed to optimize their effectiveness.

Effect of Milk Sources on Whey Protein and Fractions of Casein

Aim: Milk from Cow, Buffalo and goat was used for the study of isolation and fractionation of casein and whey proteins. Buffalo and goat milk were subjected to physicochemical attribute studies and a detailed protein profile namely total protein, casein fractions α-, β- and κ casein and whey protein were separated by urea fractionation method. The aim of this study was to compare various casein fractions isolated from cows, Buffalo and Goat milk. The method of separating casein into its fractions is based on the solubility of the individual components in urea the solution. The separation of casein fractions was carried out in dissolving molar concentration of urea and the yield of fractions and total protein percentages were calculated. Buffalo and goat milk have comparatively higher total protein and whey protein content as compared to cow milk. Maximum whey proteins found in goat milk (20.58%) whereas cow milk had the lowest whey protein (19.29%). Among casein fractions, α fraction (16.64%) was found maximum in Buffalo milk followed by cow (14.92%) and goat milk (5.42%). The goat milk showed the highest level of β casein (17.81%) whereas, the lowest concentration of β casein was found in cow milk (9.38%). Study Design: A significant contribution to the total milk production of India comes from buffalo milk and goat milk. Despite that, Buffalo milk and goat milk are not being utilized for many products because of difference in composition of different species milk and their inherent problems associated in the production of products. Place and Duration of Study: Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Dairy Science College, Hebbal, Bangalore Karnataka, India Materials: The Goat milk samples were collected from Sinchana Goat and Sheep farm, Marenahalli village (Bengaluru Rural Dist) and Buffalo milk was obtained from Country Delight Pvt. Ltd., J. P. Nagar, Bengaluru, Karnataka. Cow milk used in this investigation was collected from SEDP, Dairy Science College, Hebbal, Bengalore.. All the glassware used was soaked in chromic acid solution, repeatedly washed with water, rinsed with distilled water and dried before use. For microbiological analysis dried test tubes, conical flasks, pipettes were cotton plugged and sterilized in a hot air oven. The chemicals and reagents used in this study were mainly of analytical grade procured from Prince Laboratory Company Pvt. Limited, Bangalore. The protein molecular weight markers used for the electrophoretic study were procured from Bangalore Genei Pvt. Ltd. Methodology: Standard urea fractionation method adopted by Hipp et al was used for isolation and fractionation of caseins. Skim milk was prepared by subjecting whole milk for centrifugation, the skim milk obtained was acidified using 1 % hydrochloric acid at 200 C to obtain casein precipitate. Obtained whole casein was dissolved in 3.3 M urea at pH 7.5 and adjusted to pH 4.6 which precipitates the bulk of α casein and leaving k-caseins into soluble filtrates. Again the supernatant was adjusted to pH 4.9 diluted to 1.0 M urea and warmed to 300 C precipitating the β-caeisn. Further, addition of ammonium sulphate was used to isolate k-casein from the precipitate. Results: The sources of protein had a significant influence on the total casein, casein fractions and whey protein content of different species. The highest quantity of total caseins (34.30g/l) and whey protein (8.87 g/l) were observed in buffalo milk followed by cow (28.52 g/l) and lowest was observed in goat milk (28.45 g/l). Total protein, casein and whey protein contents were greatly affected by the source of milk. Conclusion: The significant effect of source of milk on total protein, casein, its fractions and their yield was observed. highest yield of casein (54.31%) was observed in cow milk than buffalo milk (48.95 %) and lower yield was found in goat milk. Amongst the three species, highest per cent of β casein was noted in goat milk (54.05 %) followed by buffalo (36.03%) and cow milk (34.14%). Recommendation: Rasagulla will be consumed universally by all age groups globally. Thus, the consumption of Rasagulla helps in providing overall nutritional requirements. However, the study has been conducted on a pilot scale. Moreover, commercial production may necessitate huge cost on enzymatic modification of proteins and separation peptides for value addition.

Managing malnutrition and multimorbidity in primary care: dietary approaches to reduce treatment burden.

There are many health and nutrition implications of suffering from multimorbidity, which is a huge challenge facing health and social services. This review focuses on malnutrition, one of the nutritional consequences of multimorbidity. Malnutrition can result from the impact of chronic conditions and their management (polypharmacy) on appetite and nutritional intake, leading to an inability to meet nutritional requirements from food. Malnutrition (undernutrition) is prevalent in primary care and costly, the main cause being disease, accentuated by multiple morbidities. Most of the costs arise from the deleterious effects of malnutrition on individual's function, clinical outcome and recovery leading to a substantially greater burden on treatment and health care resources, costing at least £19·6 billion in England. Routine identification of malnutrition with screening should be part of the management of multimorbidity together with practical, effective ways of treating malnutrition that overcome anorexia where relevant. Nutritional interventions that improve nutritional intake have been shown to significantly reduce mortality in individuals with multimorbidities. In addition to food-based interventions, a more 'medicalised' dietary approach using liquid oral nutritional supplements (ONS) can be effective. ONS typically have little impact on appetite, effectively improve energy, protein and micronutrient intakes and may significantly improve functional measures. Reduced treatment burden can result from effective nutritional intervention with improved clinical outcomes (fewer infections, wounds), reducing health care use and costs. With the right investment in nutrition and dietetic resources, appropriate nutritional management plans can be put in place to optimally support the multimorbid patient benefitting the individual and the wider society.

EFFECTS OF DEFICIENT DIETARY INFORMATION, WITH A FOCUS ON THE WORKING CLASS OF INDIA

Maintaining dietary equilibrium is essential for the well-being of individuals and future generations. However, a significant portion of the Indian population, specifically the working class, lack access to adequate dietary information and resources. This impedes their ability to maintain a balanced diet. This paper explores the importance of adequate nutrition, the dire ramifications of its absence, and possible solutions to this deficiency in nutrition. Using research techniques such as case studies and government data analysis, the paper investigates the current state of nutrition in India, particularly among the working class. It identifies cases of inadequate nutrition and food poisoning and their causes. The findings reveal that many Indians do not meet their daily nutritional requirements, with common deficiencies in vitamins B12 and D3, zinc, iron, calcium, folic acid, and Vitamin C. This inadequacy’s root causes are traditional dietary practices, vegetarianism, and, most importantly, the lack of dietary education and information among the working class of India. These nutritional deficits contribute to health issues such as obesity, heart disease, strokes, type 2 diabetes, and various cancers. Furthermore, food poisoning poses a significant threat to one's health, causing numerous deaths annually. Several methods of improvement for this bleak situation were deduced, such as awareness campaigns, collaborations between healthcare practitioners and schools or community centers, and the distribution of educational pamphlets in local languages, making dietary information easily accessible to the masses.