The objective of this research was to determine the difference on growth between a rice cultivar with Clearfield® technology (Only Rice 228) and a hybrid (Benja 1); to characterize nutrient uptake, distribution, accumulation and removal between these two commercial genotypes. Tests under shade house and field conditions were performed to estimate macro and micronutrient uptake patterns. Plants were sampled at nine growth stages (emergence, initiation of tillering, active tillering, initiation of panicle primordia, booting, flowering, milky, soft dough and mature grain) and divided into different organs for nutrient determination. The results showed that “Benja 1” plants (92 d) had a shorter cycle than “Only Rice 228” (OR 228) plants (118 d). “OR 228” exhibited a greater biomass production (16.575 kg ha−1 vs. 12.621 kg ha−1) in field. The nutrient acquisition was faster in the hybrid Benja 1 between tillering initiation and the milky grain stage in which the N, K, Ca, Mg, Mn, B, and Cu uptake was more evenly and highly distributed throughout these stages in both conditions. “Benja 1” showed a higher nutrient harvest index (HI). HI values above 50% (P (62%), N (61%), Cu (67%), S (55%), and Mg (52%)) were found in Benja 1 under field conditions. The results also highlight Si removal in both rice genotypes, in which Benja 1 stands out. These results provide information on the nutrient uptake and partitioning of modern rice genotypes, and give the knowledge to optimize fertilizer programs and timing recommendations for rice biomass and grain production in Colombia.