Nutrient Composition Research Articles

Production And Quality Evaluation Of Kokoro (A Maize Based Snack) From Sprouted Maize Suipplemented With African Yam Bean

“Kokoro”, a maize-based snack characterized by low protein was evaluated for nutrients composition by supplementation with African yam bean (AYBF)flour using standard analytical procedures. The maize-based snack (Kokoro) produced from this research work was a blend of unsprouted maize and sprouted maize supplemented with AYBF at different substitutional levels. The proximate content revealed that moisture content, crude protein, fat, ash and fibre as well as carbohydrate contents ranged from 9.23-1054%, 7.82-13.13%, 20.82- 31.73%, 1.52—1.96%, 2.16-2.74% and 41.21-57.14% respectively. Increase in the levels of protein, fat, ash and fibre as the level of substitution increases was observed. Functional properties revealed AYBF can be useful in food application, most especially with cereal based crops. The pasting characteristics although revealed significant variation (P<0.05) among samples, also revealed its uses in food systems. The sensory qualities showed that sample supplemented with 30% AYBF came closely to the reference kokoro, indicating consumers’ preference for supplemented kokoro that an favourably supply needed nutrients when consumed.

Effects of seasonal factors on the milk composition of dairy cows in Tafawa Balewa L.G.A, Bauchi State

This study compared the nutrient composition of milk between cattle breeds of Bunaji, Rahaji, Sokoto gudali and Adamawa gudali for both rainy and dry season. One hundred questionnaires were administered to respondents and 5% had milk yield of 3litre/day. 55% recorded 2 litre/day of milk while 40% had the least yield of 1litre/day. That early lactation produces highest milk quantity. The result of laboratory analysis showed that season have effect on nutrient composition of cow milk. It was observed that the early dry season milk composition was better for Bunaji, Rahaji, Sokoto gudali except for Adamawa gudali. For rainy season comparison, early rainy season milk composition was better for Bunaji, Sokoto gudali, Adamawa gudali except for Rahaji. Moisture content in dry season (70.08%) was lower (P>0.05) than moisture content in rainy season (87.36%) for Bunaji breed, similar result was obtained for Rahaji, Sokoto gudali and Adamawa gudali. The energy content and carbohydrate were higher (P<0.05) in the dry season for Bunaji(132.15KCal and 14.06%), Rahaji (126.20KCal and 14.55%), Sokoto gudali (148.00KCal and 22.05%) and Adamawa gudali (135.18KCal and 20.99%). The crude protein of analysed milk was slightly higher (P<0.05) in rainy season for Bunaji (5.64%), but higher in dry season for Rahaji (5.48%), Sokoto gudali (7.47%) and Adamawa gudali (6.01%). ether extract were all higher in dry season for breeds except Sokoto gudali. crude fibre was higher (5.34% Bunaji, 5.28% Rahaji, 3.45% S. gudali, 4.04% A. gudali) in dry season for all breed. Milk in all the four breeds of cattle have more of the nutrient composition in dry season at a higher level. Seasonal variation and climatic factors like rain, humidity and wind affects milk composition and cattle production in terms of pasture availability and the quality. The herders need to be encouraged to engaged in modern way of herding by possessing land for farming, they can rent it in exchange of crop residues and they can cultivate their personal farm produce and faster yielding pasture can be cultivated.

Enhancing resistant starch fraction in modified elephant foot yam starch: optimizing preparation conditions and exploring tribological properties

IntroductionElephant foot yam starch is highly susceptible to enzymatic digestion. To address this, heat-moisture treatment (HMT) has been used to modify its digestive properties and functionalities. However, when using modified starch in various food compositions, it is ultimately necessary to cook it in water-rich environments. The present work thus offers a systematic understanding of the heating and cooking procedures for modified starch, with a view to maintaining its structural and digestive properties.MethodsExperimental investigations employing X-ray diffraction, scanning electron microscopy, and enzymatic digestion have been utilized to gain insights into the characteristics of cooked starches. The interactions between amylose and amylopectin in native starch are more susceptible to disruption during cooking, which results in changes in texture and a reduction in resistant starch (RS) retention. Additionally, these preparations have been investigated by tribology to test structure and and friction.Results and discussionThe application of HMT has been observed to induce changes that stabilize the interactions between amylose and amylopectin, thereby ensuring better maintenance of granular integrity and higher RS retention under specific cooking conditions. The findings indicate that following a 2-min cooking period at 100°C, the modified elephant foot yam starch (EFYS) demonstrated enhanced granular stability, which was subsequently compromised after 10 min of cooking. Additionally, when modified elephant foot yam starch (EFYS) was heated at a temperature of 86°C for 2 and 6 min, it demonstrated superior preservation of resistant starch (RS) in comparison to a cooking time of 10 min. Moreover, the HMT starch exhibited the highest RS retention when cooked at 100°C for 2 and 6 min at approximately 39.12% and 20.23%, respectively. However, this retention decreased significantly to 1.08% after 10 min of cooking. Furthermore, the insights from tribology, when combined with the proposed naive models, enhance the understanding of how preparation conditions affect the functionalities of starches, which is crucial for designing food products with desired textural and nutritional qualities, especially in water-rich environments, where maintaining structural integrity and RS content is important.

Biological characteristics of Moolgarda cunnesius (Valenciennes, 1836) in Thua Thien Hue, Vietnam

Moolgarda cunnesius (Valenciennes, 1836) is a species of the Mugilidae family, commonly known as a delicious fish with high nutritional and economic value, making it very promising. Zoological research methods and DNA barcoding are used to study this fish. Morphological Characteristics: The Leaf mullet has 62 to 75 gill rakers on the lower limb of the first gill arch. The upper jaw is slightly curved downward, and the anterior margin of the anterior alveolar is slightly concave. The body has 37 to 43 scales. The first dorsal fin has 4 spines, while the second dorsal fin is smaller and starts behind the anal fin. Pectoral Fins: These fins are 2/3 of the head length. Anal and Pelvic Fins: These fins are small. The second dorsal and anal fins have few scales, with scales only on the anterior and basal parts. The dorsal surface is green, the flanks and abdomen are iridescent silver, and the fins are slightly dull. There is a difference in the measurement criteria between the study areas, but there is no difference between male and female groups. Mahalanobis distance analysis showed no conformational difference between male and female groups of M. cunnesius and no similarity between sexes. Nutritional biological characteristics: The food composition includes a variety of zooplankton phytoplankton benthic animals, and organic detritus. Organic humus has the highest occurrence, followed by dinoflagellates, blue-green algae, diatoms, and the lowest is arthropods. This diverse food composition illustrates that M. cunnesius is an omnivorous species. Genetic Characteristics: COI gene DNA barcoding was used to study the genetic diversity of M. cunnesius in two regions of the North and the South of Tam Giang lagoon. Amplified and sequenced 10 samples of the COI gene of M. cunnesius in Tam Giang lagoon recorded a size of 650 bp. The rate of difference in COI sequences of the 2 samples is low compared to previous studies. The similarity coefficient of M. cunnesius in the two areas of Tam Giang lagoon is very high (0.9953).

Survey on the salt usage among Fish Cracker (Keropok lekor) manufacturers in several districts of Kuala Terengganu, Malaysia

Fish crackers (keropok lekor) are known for their tastiness. Although rich in protein and iodine, it contains a high amount of salt or sodium. Therefore, this study aimed to determine the manufacturers’ practice in the preparation of keropok lekor, perception and challenges concerning salt usage, and motivation to reduce salt content. This study also aimed to estimate the sodium content in keropok lekor. Seven manufacturers of keropok lekor in Terengganu were interviewed either face-to-face or online to obtain relevant information. All interviews were recorded, transcribed, and analyzed based on a thematic analysis framework. The recipes of keropok lekor were analyzed using the Nutritionist Pro software to determine the nutritional and sodium content, calculated based on the Malaysian and ASEAN Food Composition Tables. Based on the interviews, most manufacturers (90%) supported the effort to reduce salt in keropok lekor. However, the effort was met with several challenges, such as customer acceptance and negative perception of the taste, decreased sales, and increased material costs. The enabling factors include maintaining the original flavor of keropok lekor, customer requests, marketing opportunities, and the availability of salt substitutes. Support for marketing and financial technology know-how is also necessary. An estimate of 606.43 mg/100g of sodium content in keropok lekor was obtained in this study, which was mainly derived from the usage of sodium chloride and monosodium glutamate (MSG) during the preparation. This study revealed the high salt content in keropok lekor due to the addition of salt and seasonings. There is a potential to reduce salt content in keropok lekor with appropriate support provided to the manufacturers. Nevertheless, further research is needed to produce keropok lekor with reduced salt content, improved sensory, and enhanced microbial and physicochemical characteristics, which can improve customer perception.

The role of zeolite and mineral fertilizers in enhancing Table Beet (Beta vulgaris L.) productivity in dark chestnut soils of Southeast Kazakhstan

This study evaluated the effectiveness of zeolite, both alone and in combination with mineral fertilizers, in improving the yield and quality of table beets (Beta vulgaris L.) grown in dark chestnut soils of southeast Kazakhstan. The research was conducted at the Kazakh Research Institute of Horticulture during the 2022-2023 growing seasons using a randomized complete block design with six treatments: control (no fertilizers), zeolite 2 t/ha, N45P45K45 (single dose of mineral fertilizers), N90P90K90 (double dose of mineral fertilizers), zeolite 2 t/ha + N45P45K45, and zeolite 2 t/ha + N90P90K90, replicated three times. The application of zeolite significantly improved soil physical properties, such as water permeability and soil density, enhancing root development and water retention. Nutrient availability, particularly nitrate nitrogen and mobile phosphorus, increased significantly in zeolite-treated plots. The combination of zeolite and mineral fertilizers resulted in the highest improvements, with nitrate nitrogen content reaching 40.5 mg/kg and mobile phosphorus 89.2 mg/kg. Moreover, zeolite reduced heavy metal concentrations, particularly cadmium, by 50% compared to the control. Table beet yield significantly increased with zeolite application, with the highest yield of 62.7 t/ha achieved with 2 t/ha zeolite combined with double dose N90P90K90 fertilizers, compared to 42.8 t/ha in the control. Marketable yield also improved, indicating better crop quality. Nutrient composition of the beets improved, with increased dry matter content (21.9%) and reduced nitrate content (240 mg/kg) in zeolite-treated variants. In conclusion, zeolite, especially when combined with mineral fertilizers, effectively enhances soil health, nutrient availability, and table beet yield and quality.

Study on Rumen Degradability and Intestinal Digestibility of Mutton Sheep Diets with Different Concentrate-to-Forage Ratios and Nonfiber Carbohydrates/Neutral Detergent Fiber Ratios.

This study was conducted to investigate the rumen degradability and intestinal digestibility of mutton sheep diets different in concentrate-to-forage ratio, NFC/NDF, and ingredient combination, providing a guideline for the selection of a fattening diet for mutton sheep. Twenty-eight diets composed of four raw material combinations and seven concentrate-to-forage ratios and four three-year-old mutton sheep with permanent rumen fistulas were used in the experiments. The nutrient composition of the diets was first analyzed, and then an in situ method and in vitro three-step method were separately used to measure the rumen degradability and intestinal digestibility, mainly focusing on the effects of dietary concentrate-to-forage ratio and NFC/NDF as well as the effects of soybean meal and soybean meal replacement and peanut vine and peanut vine replacement. The results showed that a dietary concentrate-to-forage ratio of 70:30~80:20 and an NFC/NDF ratio of 1.5~2.0 are recommended for fattening mutton sheep, and low-cost cottonseed meal and rapeseed meal can be feasible alternative protein sources to soybean meal. In addition, the nutritional values of sunflower seed hulls and rice hulls for mutton sheep are lower than that of peanut vine. Such a study can provide practical guidelines for enterprises and farmers, being of important significance for the high-quality development of the mutton sheep industry.

How do plant-based milks compare to cow's milk nutritionally? An audit of the plant-based milk products available in Australia.

This cross-sectional study aims to explore the nutritional composition, cost, country of origin and fortification status of plant-based milk products available for purchase in Illawarra supermarkets and make various comparisons between types of plant-based milks and cow's milk. Plant-based milk information was collected from nutrition information panels on packaging and manufacturer websites. Product ingredient lists, including fortifiers, were analysed to estimate the nutrient composition of the identified plant-based milks, including nutrients beyond those listed by manufacturers. Descriptive statistics were used to summarise the characteristics of the plant-based milks identified in the audit. For non-normally distributed data, a Kruskal-Wallis H test with pairwise multiple comparisons and a Bonferroni adjustment were undertaken to explore the differences between various types of plant-based milk and cow's milk. One hundred twenty-nine plant-based milk products were identified in the audit, primarily almond, oat and soy-based beverages. Of these, 80.6% were fortified with calcium; however, fortification with other micronutrients was less common, ranging from 27.1% being fortified with vitamin B12 and 3.1% being fortified with iodine. The median plant-based milk cost was AU$3.5/L (Q1-Q3: AU$2.8-4.5/L) and 87.6% of products were Australian made. Overall, particularly due to low fortification rates, plant-based milks identified in the audit had significantly lower levels of protein, sugar, iodine, phosphorus, zinc and vitamins A, B2 and B12 compared to cow's milk. However, there was no significant difference in protein content between soy milk and cow's milk. The nutritional content of plant-based milks identified in this audit varied, and in most instances, Australian plant-based milks were found to be nutritionally different to cow's milk.

(S)-2,5-dioxopyrrolidin-1-yl-1-(4,6-dimethoxy-1,3,5-triazin-2-yl)pyrrolidine-2-carboxylate derivatization combined with UPLC/ESI-MS/MS as a powerful method for dipeptide determination in foods: An application example in fermented cocoa beans

Dipeptides play a crucial role in food flavor, as well as in physiological and nutritional functions. However, as comprehensive methods for determining dipeptides are still under development, the dipeptide composition of foods remains largely unexplored. This study proposed a novel analytical method for dipeptides by applying an amino acid determination technique we previously developed: (S)-2,5-dioxopyrrolidin-1-yl-1-(4,6-dimethoxy-1,3,5-triazin-2-yl)pyrrolidine-2-carboxylate (DMT-(S)-Pro-OSu) derivatization coupled with ultra-performance liquid chromatography–electrospray ionization tandem mass spectrometry (UPLC/ESI-MS/MS). The DMT-(S)-Pro-OSu derivatization of dipeptides improved both separation in LC and detection sensitivity in MS, allowing for the optimized acquisition of m/z and elution times for 313 available dipeptides, and enabling the absolute quantification of 126 dipeptides in a simultaneous analysis. Subsequent analysis of fermented cocoa beans detected and quantified 42 dipeptides, revealing distinct dipeptide profiles depending on the origin of the beans. Additionally, by conducting in silico analysis of the elution times for 313 dipeptides, a machine learning model was constructed that accurately predicts dipeptide elution times. The DMT-(S)-Pro-OSu-UPLC/MS/MS method and the elution time prediction model developed in this study are expected to contribute to the understanding of dipeptide composition in foods.

Bacterial cell size modulation along the growth curve across nutrient conditions.

Under stable growth conditions, bacteria maintain cell size homeostasis through coordinated elongation and division. However, fluctuations in nutrient availability result in dynamic regulation of the target cell size. Using microscopy imaging and mathematical modelling, we examine how bacterial cell volume changes over the growth curve in response to nutrient conditions. We find that two rod-shaped bacteria, Escherichia coli and Salmonella enterica, exhibit similar cell volume distributions in stationary phase cultures irrespective of growth media. Cell resuspension in rich media results in a transient peak with a five-fold increase in cell volume ≈ 2h after resuspension. This maximum cell volume, which depends on nutrient composition, subsequently decreases to the stationary phase cell size. Continuous nutrient supply sustains the maximum volume. In poor nutrient conditions, cell volume shows minimal changes over the growth curve, but a markedly decreased cell width compared to other conditions. The observed cell volume dynamics translate into non-monotonic dynamics in the ratio between biomass (optical density) and cell number (colony-forming units), highlighting their non-linear relationship. Our findings support a heuristic model comparing modulation of cell division relative to growth across nutrient conditions and providing novel insight into the mechanisms of cell size control under dynamic environmental conditions.

The Impact on People who Trade on the Fish Species ‘Limnothrissa miodon’ - Kapenta for their Livelihood: Practical Perceptions of Traders of Siavonga

Lake Kariba is the world’s largest man-made lake and reservoir by volume. It lies 1300km upstream from the Indian Ocean, along the border between Zambia and Zimbabwe. Lake Kariba was filled between 1958 and 1963 following the completion of the Kariba dam at its north eastern end flooding the Kariba Gorge on the Zambezi River. Culturally controversial, the local people believed that the construction of the dam would anger the Zambezi river god Nyami-nyami, who rise up and cause the dam would wall to collapse destroying the bridge as well as resulting in floods of catastrophic proportions. It is believed that the Nyami-nyami lives under a rock close to the Kariba dam wall. The rock is called ‘Kariwa’ meaning trap as the local people believed if you canoed past its location you would be sucked down in whirlpool never to be seen again. This where the name Kariba originates from, laced with the local mythology. In the late 1960s a type of fish Limnothrissa miodon locally known as Kapenta was introduced in Lake Kariba from lake Tanganyika. Kapenta is a small sardine fish which supports a large and viable fishery for Zimbabwe and Zambia. Kapenta, a type of fish also known as fresh water sardine has several chemical compositions that help reduce the risk of various diseases including prostate cancer. A recent chemical composition analysis of Kapenta by the National Food and Nutrition Commission (NFNC) shows a great variation in the nutrient composition for the dry and fresh Kapenta. NFNC principal nutritionist Mr. Musonda Mofu says dry Kapenta contains 209 calories of food energy compared with 85 calories of food energy in fresh Kapenta. In addition dry Kapenta contains 63 grammes of protein and only 16 grams of protein in fresh Kapenta. The paper reflects on the declining population of this small nutrient rich fish due to over-harvesting, climatic changes with increasing temperatures and with the decreasing levels of water storage. A cumulative study on the pattern of nurturing the fish for economic and social flexibility is the main concern of this paper.

Impact of cultural, nutrition, and lifestyle transition on human milk composition between the Orang Asli communities in Peninsular Malaysia: A study protocol.

Background: Human milk provided by healthy, well-nourished mothers represents the best food available for infants. Human milk is species-specific and recommended as the sole source of nutrition for the first 6 months of life and is an important food source until the infants reach 2 years of life. Unlike infant formula, which has a standardized composition, human milk composition varies among and within women. Aim: While many studies of human milk composition have been conducted, none have been done among the Orang Asli people in Malaysia. Hence, this study protocol is designed to determine the human milk composition of the Orang Asli in Peninsular Malaysia and how the transition of culture, nutrition, and different lifestyle experiences will affect the nutrient content of their milk. Methods: Quantitative (interviewer-administered questionnaire) and qualitative (focus group discussion) approaches will be employed in this study and in addition, laboratory works will also be carried out to analyze the nutrient composition of the milk samples collected from various Orang Asli subethnic groups in Peninsular Malaysia. Conclusion: Standardized, multipopulation studies of human milk composition are sorely needed to create a rigorous, comprehensive reference inclusive of nutrients and other factors. The establishment of any dietary parameters positively affecting the nutrient content of human milk may help design healthcare strategies in Malaysia to improve the quality of human milk and therefore the infants' health status.

Repercussion of Controlled Decomposition Process on Carbon Dynamics and Quality Evaluation in Chicken Manure Pellets

An incubation experiment was conducted to determine the decomposition potential of the chicken manure pellets and to know the nutrient composition. The chicken manure pellets produced from the bedding material of commercially grown broiler farm was tested for decomposition rate through CO2 evolution and nutrient content. The rate of decomposition of poultry manure pellets (CO2 emission) was less during the first week of incubation and linearly enhanced during 2nd and 3rd week after incubation. The rate of emission of CO2 has been reached maximum (150 mg of CO2 per gram per day during the 4th week of incubation. The enhanced CO₂ evolution indicated addition of more organic matter content and balanced steady release of nutrients on incorporation in to soil.

Native Forbs Provide Pollinator Resources and Improve Forage Nutrient Composition, Animal Performance, and Pasture Productivity

Pollinator declines and expectations for more sustainable agriculture, including pasture-based enterprises, bring attention to strategies to enhance the habitat value of grazing lands. We evaluated native warm-season grass (NWSG) pastures with (FORB) and without (CONT) interseeded native forbs in 2021–2023. An analysis was conducted using R with the significance set at p ≤ 0.05. The grass appeared to be weakened predominantly by grazing management practices. Forb density and mass had an inverse relationship in seasons two and three. Total forage mass declined in response to increased grazing days and weakened stands. The forage nutritive compositions differed, with more stable, season-long crude protein and lower fiber concentrations in late-season FORB, which supported higher bodyweight gains and season-long average daily gain. Black-eyed Susan (Rudbeckia hirta; BESU), lanceleaf coreopsis (Coreopsis lanceolata; LCOR), and showy ticktrefoil (Desmodium canadensis; STTF) were the most abundant forbs, and BESU, LCOR, and purple coneflower (Echinacea purpurea; PURC) produced long flowering windows. Cattle grazed STTF, cup plant (Silphium perfoliatum; CUPP), and oxeye sunflower (Helopsis helianthoides) the most. Under continuous stocking, a blend of BESU, LCOR, PURC, STTF, and CUPP produced acceptable cattle gains and provided pollinator resources, suggesting that this model may be a viable means to enhance the sustainability of pastures.

Impacts of Bacillus amyloliquefaciens and Trichoderma spp. on Pac Choi (Brassica rapa var. chinensis) grown in different hydroponic systems.

Soilless production systems (i.e hydroponics, aeroponics, aquaponics) have become commonplace in urban settings and controlled environments. They are efficient nutrient recyclers, space savers, and water conservers. However, they lack high levels of biological richness in the root microbiome when compared to soil production systems, which may affect plant health and nutrient uptake. To address this issue and incorporate more sustainable practices, beneficial microorganisms (i.e. Trichoderma spp., Bacillus sp.) can be added in the form of biofertilizers. However, many factors affect impacts of microorganisms and their interactions with plants. In this experiment, Black Summer Pac Choi (Brassica rapa var. Chinensis) was grown for two trials in a Deep-Water system (DWS) or a Nutrient Film Technique system (NFT) with commercial biofertilizers containing Trichoderma spp., Bacillus amyloliquefaciens, a combination of both, and a control. Plant physiology, nutrient composition, and nutrient uptake efficiency (NUE) were generally negatively affected by Trichoderma spp. both growing systems, indicating that Trichoderma may not be recommended for hydroponic production. However, Bacillus amyloliquefaciens showed promise as an effective biofertilizer in the NFT systems and had a positive influence on NUE in DWS.

In Vitro Human Gastrointestinal Digestibility and Colonic Fermentation of Wheat Sourdough and Yeast Breads.

Bread can vary in textural and nutritional attributes based on differences in the bread making process (e.g., flour type, fermentation agent, fermentation time). Four bread recipes (BRs) made with sourdough preferments (BR1, white flour; BR2, whole grain flour) or regular yeast breads (BR3, white flour; BR4, whole grain flour) were evaluated for texture, digestibility, and their effect on the metabolic activity and composition of the gut microbiota using texture profile analysis (TPA) coupled with in vitro upper gastrointestinal (GIT) digestion and colonic fermentation (Colon-on-a-plate™ model), using fecal samples from eight healthy human donors. TPA revealed significantly higher values for hardness, fracturability, gumminess, and chewiness, and significantly lower values for springiness, cohesiveness, and resilience with whole grain versus white breads (all p < 0.001); values for springiness, cohesiveness, and resilience were significantly higher for sourdough versus yeast bread (p < 0.001). Nutrient composition and bioaccessibility were generally comparable between sourdough and yeast bread with similar flours. Following simulation of upper GIT digestion, all BRs demonstrated good digestibility of minerals, carbohydrates, and proteins. Colonic fermentation revealed changes in gut microbiota composition, significant increases in short-chain fatty acids, and a significant decrease in branched short-chain fatty acids with all BRs versus a blank. Overall, new insights into wheat bread digestibility and colonic fermentation were provided, which are important aspects to fully characterize bread nutritional profile and potential.

A superior chitin product: Black soldier fly larvae chitin, beneficial to growth performance, muscle quality and health status of largemouth bass Micropterus salmoides, in comparison to shrimp chitin

We appraised the effects of black soldier fly larvae chitin (BSFC) with four levels (0.4 %, 0.8 %, 1.2 % and 1.6 %) and shrimp chitin (SC) with three levels (0.4 %, 0.8 % and 1.6 %) in diets on the growth performance, nutrient composition and health status of largemouth bass (8.00 ± 0.29 g) fed for 56 days. The results showed that the dietary 0.4 % BSFC distinctly increased the specific growth rate and weight gain rate of fish (P < 0.05), and evidently promoted the deposition of C22:1n-9, C24:1n-9 and increased total monounsaturated fatty acid content in the muscle (P < 0.05). BSFC and SC distinctly stimulated the secretion of β-N-acetylglucosaminidase in the hindgut (P < 0.05). The antioxidant status of fish was improved by BSFC and SC, which was attributed to the decreased malonaldehyde contents in the muscle and midgut and the increased total superoxide dismutase and catalase activities in the midgut. The 1.2 %–1.6 % BSFC and 0.8 %–1.6 % SC enhanced the non-specific immunity of fish, which was attributed to the marked promotion of lysozyme, acid phosphatase and alkaline phosphatase activities by BSFC and the distinct enhancement of lysozyme activity by SC in the serum or midgut (P < 0.05). BSFC and SC notably reduced the abundance of potential pathogenic bacterium Vibrio and Photobacterium (P < 0.05), and 0.4 %, 1.2 %, 1.6 % BSFC and 0.4 % SC markedly increased the abundance of probiotic bacterium Cetobacterium (P < 0.05), and the BSFC and SC of 0.8 % significantly increased the abundance of the probiotics Bifidobacterium and Bacillus respectively (P < 0.05). The increase of acetic acid, propionic acid, butyric acid and valeric acid concentrations in the hindgut might be related to the increase of intestinal probiotics, and 0.8 %–1.6 % BSFC and 0.4 % SC obviously increased the total short-chain fatty acid levels in the hindgut (P < 0.05). BSFC and SC remarkably reduced muscle fiber area and diameter (P < 0.05), while fiber densities were significantly increased under 0.4 %, 0.8 % BSFC and 0.4 % SC supplementation (P < 0.05), which was accordant with increased mRNA expression levels of myod, myf-5, smad-2, tgf-β1 and decreased mRNA expression levels of myos in the muscle. Collectively, BSFC and SC had no negative effect on the growth of largemouth bass, and improved the health status of fish. The dietary 0.4 % BSFC supplementation was beneficial to fish growth and muscle quality, and BSFC was a superior chitin product in comparison to SC.

Assessment of the Antioxidant Potential of Blackthorns and Hawthorns: Comparative Analysis and Potential Use in Ruminants’ Nutrition

The food industry is intensifying its effort to enrich food composition in various nutrients through animal feeding, but these challenges can be limited by the costly feed resources, water scarcity, and pesticide pollution, making it crucial to explore alternative feedstuffs with fewer requirements. Blackthorns and hawthorns are characterized by their rich phytochemical and antioxidant profiles, suggesting their potential to enhance the performance of ruminants though the supply of bioactive substances. Our study revealed their rich composition of micronutrients; hawthorns showed a remarkable amount of polyunsaturated fatty acids (57.23 g FAME/100 g total FAME), particularly omega-3 and omega-6, while blackthorn presented higher concentration of monounsaturated fatty acids, specifically oleic acid (56.99 g FAME/100 g total FAME). In terms of lipo-soluble antioxidants, blackthorn exhibited higher levels of xanthophyll and vitamin E (123.83 mg/kg DM), including its isomers (alpha, gamma, and delta). Concerning the water-soluble antioxidants, hawthorns showed elevated composition of the total content of flavonoids and polyphenols, comparing with blackthorn. Moreover, hawthorns showed a high antioxidant capacity, as assessed through DPPH, ABTS, and TAC analyses. In terms of the scavenging capacity of blackthorn and hawthorn against superoxide radicals, blackthorn had higher radical scavenging potential against superoxide radicals, compared to hawthorn.

Unlocking the Potential of Food Waste: A Review of Multifunctional Pectins.

This review comprehensively explores the multifunctional applications of pectins derived from food waste and by-products, emphasizing their role as versatile biomaterials in the medical-related sectors. Pectins, known for their polyelectrolytic nature and ability to form hydrogels, influence the chemical composition, sensory properties, and overall acceptability of food and pharmaceutical products. The study presents an in-depth analysis of molecular parameters and structural features of pectins, such as the degree of esterification (DE), monosaccharide composition, galacturonic acid (GalA) content, and relative amounts of homogalacturonan (HG) and rhamnogalacturonan I (RG-I), which are critical for their technofunctional properties and biological activity. Emphasis is placed on pectins obtained from various waste sources, including fruits, vegetables, herbs, and nuts. The review also highlights the importance of structure-function relationships, especially with respect to the interfacial properties and rheological behavior of pectin solutions and gels. Biological applications, including antioxidant, immunomodulatory, anticancer, and antimicrobial activities, are also discussed, positioning pectins as promising biomaterials for various functional and therapeutic applications. Recalled pectins can also support the growth of probiotic bacteria, thus increasing the health benefits of the final product. This detailed review highlights the potential of using pectins from food waste to develop advanced and sustainable biopolymer-based products.

Transcriptome and Metabolome Based Mechanisms Revealing the Accumulation and Transformation of Sugars and Fats in Pinus armandii Seed Kernels during the Harvesting Period.

Pinus armandii seed kernel is a nutrient-rich and widely consumed nut whose yield and quality are affected by, among other things, harvesting time and climatic conditions, which reduce economic benefits. To investigate the optimal harvesting period of P. armandii seed kernels, this study determined the nutrient composition and seed kernel morphology and analyzed the gene expression and metabolic differences of P. armandii seed kernels during the harvesting period by transcriptomics and metabolomics. The results revealed that during the maturation of P. armandii seed kernels, there was a significant increase in the width, thickness, and weight of the seed kernels, as well as a significant accumulation of sucrose, soluble sugars, proteins, starch, flavonoids, and polyphenols and a significant decrease in lipid content. In addition, transcriptomic and metabolomic analyses of P. armandii seed kernels during the harvesting period screened and identified 103 differential metabolites (DEMs) and 8899 differential genes (DEGs). Analysis of these DEMs and DEGs revealed that P. armandii seed kernel harvesting exhibited gene-metabolite differences in sugar- and lipid-related pathways. Among them, starch and sucrose metabolism, glycolysis, and gluconeogenesis were associated with the synthesis and catabolism of sugars, whereas fatty acid degradation, glyoxylate and dicarboxylic acid metabolism, and glycerophospholipid metabolism were associated with the synthesis and catabolism of lipids. Therefore, the present study hypothesized that these differences in genes and metabolites exhibited during the harvesting period of P. armandii seed kernels might be related to the accumulation and transformation of sugars and lipids. This study may provide a theoretical basis for determining the optimal harvesting time of P. armandii seed kernels, changes in the molecular mechanisms of nutrient accumulation, and quality directed breeding.