Nutraceutical Delivery Research Articles

Kokum Butter and Sesame Oil-Based Oleogels as Novel Nutraceutical Delivery Systems

Kokum Butter and Sesame Oil-Based Oleogels as Novel Nutraceutical Delivery Systems

Integrated approaches in nutraceutical delivery systems: optimizing ADME dynamics for enhanced therapeutic potency and clinical impact

Abstract Objectives Nutraceuticals is a general term for a variety of bioactive compounds such as polyphenols and omega-3 fatty acids which have shown promising ability in the field of health. Efficacy of these drugs somewhat relies on their absorption, distribution, metabolism, and excretion (ADME) processes in the body. Methods This review provides a comprehensive understanding of ADME principles blended with the pharmacological insights into design customized nutraceutical interventions dependent on individual well-being. This review discusses absorption dynamics starting from ingestion and excretion and further examines how they relate to a therapeutic outcome. In addition, special focus is paid to breakthrough methods like artificial intelligence and multidisciplinary cooperations among other ways of improving nutraceutical science. Both nanotechnology and encapsulation techniques, together with other novel approaches, aim at improving the bioavailability of foods. This is important for the continuing advancements in food engineering. Key findings Any combination of such strategies will ensure effectiveness of nutraceuticals in personalized medicine, which in turn enables better lifestyle choices for the population. Conclusions It is crucial to add ADME-understanding to pharmacology-based insights into make nutraceutical adjustments according to the situation. Innovations in the forms of AI and multidisciplinary cooperation remain crucial in the progress of nutraceutical science. Methods like nanotechnology and encapsulation are powerful tools of improving bioavailability and tailor-made medicine, which in turn, will be responsible for healthier lives.

Enhancing Nutraceutical Bioavailability with Bilosomes: A Comprehensive Review

Nutraceuticals, encompassing vitamins, minerals, and dietary supplements, play a pivotal role in promoting health and wellness. However, their therapeutic efficacy is often hindered by poor bioavailability. Bilosomes, a burgeoning lipid-based nanocarrier, have emerged as a promising solution to overcome this challenge. This comprehensive review explores the potential of bilosomes as carriers for enhancing the bioavailability of nutraceuticals, shedding light on their formulation, characterization, mechanisms of action, safety considerations, and future prospects. The review begins by delineating the significance of nutraceuticals in promoting well-being and discusses the obstacles associated with their delivery. It subsequently introduces bilosomes, lipid vesicles equipped with bile salts, and elucidates their advantages over traditional delivery systems. A comparative analysis with other lipid-based carriers underscores the unique attributes that make bilosomes an attractive choice for nutraceutical delivery. A deeper dive into bilosome formulation and characterization provides insights into lipid composition, encapsulation techniques, and physicochemical characterization methods. Factors influencing bilosome stability and bioavailability, including size, surface charge, and interactions with physiological barriers, are comprehensively reviewed. Safety and toxicological considerations related to bilosome-based nutraceutical delivery are meticulously examined, drawing from preclinical and clinical studies. Regulatory aspects pertinent to bilosome incorporation in nutraceutical products are outlined. Future perspectives delve into emerging trends, formulation challenges, and the potential for personalized nutrition with bilosomes. In conclusion, this review underscores the transformative potential of bilosomes in enhancing nutraceutical bioavailability, opening new vistas in the field of personalized nutrition and health promotion.

Nutraceutical delivery vehicles: enhanced stability, bioavailability

Nutraceutical delivery vehicles: enhanced stability, bioavailability

Liposomal Freight and Their Advanced Microstructure Characterization Techniques for Food and Nutraceutical Delivery

Liposomal Freight and Their Advanced Microstructure Characterization Techniques for Food and Nutraceutical Delivery

Effects of microencapsulated phenethyl isothiocyanate on gastrointestinal cancer cells and pathogenic bacteria

Gastrointestinal cancers remain a global health burden, demanding more effective prevention and treatments. Phenethyl isothiocyanate (PEITC), a compound derived from cruciferous vegetables, stands out as a promising nutraceutical agent due to its chemopreventive and therapeutic properties. However, its therapeutic translation remains limited mainly due to its poor water solubility and rapid metabolism. Herein, we encapsulated PEITC into biocompatible chitosan-based microparticles with an extra virgin olive oil core to improve its bioavailability and stability. Pure PEITC's biocompatibility and microencapsulated PEITC's stability and antibacterial activity were evaluated. The antibacterial activity analysis showed microencapsulated PEITC as a promising antibacterial agent against gastrointestinal pathogenic bacteria (two Gram-positive and two Gram-negative). The impact of both pure and microencapsulated PEITC was assessed on gastrointestinal cancer cells (MKN45 gastric cancer and SW48 colon cancer cell lines). PEITC exhibited threshold or hormetic dose-dependent toxicity in colon fibroblasts and decreased gastric cancer cells' migration capacity, enhanced upon encapsulation into microparticles. In addition, microencapsulated PEITC induced downregulation of phosphorylated AKT, FAK, and ERK1/2 proteins, disrupting motility signaling pathways and tubulin expression. These findings suggest that the delivery of PEITC via chitosan-based microparticles holds promise as a nutraceutical delivery strategy against gastrointestinal disorders that predispose to cancer.

Role of egg white protein particles’ structure and interface profiles in tailoring high-internal-phase Pickering emulsions with desired applicability

Tailoring food-grade high-internal-phase Pickering emulsions (HIPPEs) with desired applicability has been extremely hindered due to the limited stabilization mechanism deciphering. This study illustrated the specific role of egg white protein (EWP) particlesꞌ structure and interface distribution to the stabilization of HIPPEs. Herein, gelation induced EWP aggregation into its microgel particles (EWPG) with apparent conformation transition (α-helix to β-sheet) and surface activity improvement (e.g., hydrophobicity, interface tension, and wettability). Besides, larger EWPG tended to sparsely absorb on the HIPPEsꞌ interface and achieve admirable long-term stability (6-month storage and 18-days lipid oxidation), owing to higher particle rigidity and steric repulsion. Comparably, EWP (small, soft, and rough) was superior in ready-to-use HIPPEs with centrifugation, freeze-thawing, and nutraceutical delivery demands, accompanied by a stronger rattan-like interfacial network with higher viscoelasticity. Overall, this research would facilitate the facile design of food matrices (e.g., EWP)-based HIPPEs via the gelation-tailored balance of particle structure and interface distribution.

Formation of pea protein amyloid-like nanofibrils-derived hydrogels mediated by epigallocatechin gallate

This study investigated the interaction between pea protein amyloid-like nanofibril and epigallocatechin gallate, constructed and characterized the novel pea protein nanofibrils-derived hydrogel mediated by epigallocatechin gallate, and researched the functionalities of the hydrogel. Epigallocatechin gallate remodeled the structure of pea protein nanofibrils, and a stable and strong hydrogel was formed at a relatively low protein concentration (4.5%). Additionally, the hydrogels exhibited various surface structures and hydrogel properties dependent on the mass ratio. Strongest gel strength (51 g) was attained at 0.25 epigallocatechin gallate/pea protein nanofibrils mass ratio. Whereas, the hydrogels exhibited the highest water holding capacity (87%) at 0.05 mass ratio. The primary driving forces in the formation and maintaining of the hydrogels were hydrophobic interactions and ionic bonds. Progressive rise of β-sheet content of pea protein nanofibrils occurred increasing epigallocatechin gallate concentration. This hydrogel holds great potential for applications in food processing, targeted delivery of nutraceuticals and biomedicine.

Development of pea protein nanoparticle/hydrolyzed rice glutelin fibril emulsion gels for encapsulation of curcumin

The potential of using emulsion gels stabilized by binary plant protein nanoparticle mixtures for the encapsulation and delivery of lipophilic nutraceuticals was evaluated. The particle characteristics, physical stability, water diffusivity, microrheology, large amplitude oscillating shear (LAOS) properties, and in vitro digestion of emulsion gels prepared by different ratios of hydrolyzed rice glutelin fibrils (HRGFs) and pea protein nanoparticle (PNP) were characterized. The emulsion gel with P/H = 2:1 (0.84 μm) exhibited the best storage stability and freeze-thaw stability, as seen by the smaller oil droplet size (1.02 and 1.42 μm, respectively). Low-field pulsed NMR indicated that the majority of water in samples was highly mobile. All the samples were predominantly elastic-like materials. The P/H 2:1 emulsion gel had the lowest FI value (6.21 × 10−4 Hz), the highest MVI value (5.57 s/nm2), G′/ G″ values and enclosed area, showing that it had denser 3D network structures, higher stiffness values, and a high sensitivity to changes in strain. Additionally, P/H 2:1 emulsion gel had a relatively high lipid digestibility (96.1 %), curcumin bioaccessibility (58.9 %), and curcumin stability (94.2 %). This study showed that emulsion gels stabilized by binary protein nanoparticle mixtures (PNP/HRGF) have potential as edible delivery systems for lipophilic nutraceuticals.

Chicken Egg White Gels: Fabrication, Modification, and Applications in Foods and Oral Nutraceutical Delivery.

Chicken egg white (EW) proteins possess various useful techno-functionalities, including foaming, gelling or coagulating, and emulsifying. The gelling property is one of the most important functionalities of EW proteins, affecting their versatile applications in the food and pharmaceutical industries. However, it is challenging to develop high-quality gelled foods and innovative nutraceutical supplements using native EW and its proteins. This review describes the gelling properties of EW proteins. It discusses the development and action mechanism of the physical, chemical, and biological methods and exogenous substances used in the modification of EW gels. Two main applications of EW gels, i.e., gelling agents in foods and gel-type carriers for nutraceutical delivery, are systematically summarized and discussed. In addition, the research and technological gaps between modified EW gels and their applications are highlighted. By reviewing the new modification strategies and application trends of EW gels, this paper provides insights into the development of EW gel-derived products with new and functional features.

Biological Activities of Citrus-Derived Extracellular Vesicles on Human Cells: The Role of Preservation.

Extracellular vesicles (EVs) have been identified as important mediators for cell-to-cell communication. Citrus-based EVs in particular offer an excellent platform for nutraceutical delivery systems, as their endemic cargo includes micronutrients (e.g., ascorbic acid), which contribute to their antioxidant capacity. Despite being extensively investigated as to their therapeutic and diagnostic potential, their cargo is inherently unstable and thus directly affected by their storage and preservation. In this study, EVs were isolated from citrus fruit using tangential flow filtration and evaluated for their physicochemical characteristics, antioxidant activity and effects on human cells. To assess how their isolation and preservation methods affect these properties, the EVs were tested immediately after isolation (from fresh and freeze-thawed juices) or following freeze-drying. A measurable biological effect of cryoprotection on citrus-derived EVs was evident, whether during or after isolation. This was more pronounced in the cell-based assays, ranging from -4% to +32% in human skin fibroblast proliferation. Nevertheless, the effects on human cancer cells varied depending on the cell line. Although these results should be considered preliminary observations, subject to further investigation, it is safe to state that any type of preservation is expected to impact the EVs' biological activity.

Effects of different solid particle sizes on oat protein isolate and pectin particle-stabilized Pickering emulsions and their use as delivery systems

Oat protein isolate (OPI)/high methoxyl pectin (HMP) complexes (OPP) were prepared to stabilized Pickering emulsions and applied as nutraceutical delivery systems. The different mass ratios and pH changed the interactions between OPI and HMP that caused the different size of OPP. Specifically, smaller particle size of OPP (125.7–297.6 nm) were formed when hydrophobic interactions along with electrostatic forces predominant in OPP (OPI:HMP = 3:1, pH 4, 5). Among these particles, OPP-2 could stabilize Pickering emulsion efficiently through formation of dense interfacial film, which exhibited the highest apparent viscosity and the smallest average droplet size (23.39 μm). Moreover, OPP-2 stabilized Pickering emulsions with superior stability not only exhibited higher encapsulation efficiency of 85.63%, but also could control curcumin release in simulated gastrointestinal fluids to improve curcumin's bioaccessibility. These results verified the possibility of OPP to be a Pickering emulsions stabilizer, and also identified its potential to be a stable delivery system for bioactive compounds.

The Therapeutic Potential of Harpagophytum procumbens and Turnera subulata and Advances in Nutraceutical Delivery Systems in Neurodegenerative Diseases

This review article covers the therapeutic potential of the plants Harpagophytum procumbens and Turnera subulata in the treatment of neurodegenerative diseases. Despite the recognition of their beneficial properties, there is notable shortage of specific clinical and in vitro studies on these species regarding neurodegenerative diseases. Compounds such as harpagosides and vite-xin-2-O-rhamnoside, found in Harpagophytum procumbens and Turnera subulata, respectively, as well as other antioxidants and anti-inflammatory agents, are associated with mechanisms of action that involve reducing oxidative stress and modulating the inflammatory response, indicating their therapeutic potential in these pathologies. Additionally, the use of nutraceuticals derived from medicinal plants has emerged as a promising approach, offering natural therapeutic alternatives. However, the pressing need for studies focusing on the pharmacokinetics, safety, and pharmacological interactions of these extracts for the treatment of neurodegenerative diseases is emphasized. This review also evaluated advances in nutraceutical delivery systems, highlighting technological innovations that can optimize the precise delivery of these compounds to patients. Such findings highlight the gaps in the study of these plants for the treatment of neurodegenerative diseases and, at the same time, the potential for opening new perspectives in the treatment of neurodegenerative diseases, providing expectations for innovative solutions in this critical domain of medicine.

Novel nutraceutical delivery system utilizing a bigel formulated with sesame oil, kokum butter, and pectin.

The online version contains supplementary material available at 10.1007/s10068-024-01559-3.

Transglutaminase-crosslinked cold-set pea protein isolate gels modified by pH shifting: Properties, structure and formation mechanisms

The poor gelation properties of pea protein isolate (PPI) limit their use in food industries. In the present study, PPI was modified by pH-shifting combined with heat treatment, followed by cold-set gelation of PPI gels induced by transglutaminase. Both pH 2–3 and pH 10–12 promoted the unfolding of PPI molecules and the transformation of secondary structures from ordered to disordered. In particular, PPI molecules with pH 12-shifting tended to expand and unfold, which resulted in the exposure of internal active groups and improvement in surface hydrophobicity. Accordingly, after the addition of 50 U/g transglutaminase, PPI gels with pH 12-shifting and heating treatment exhibited homogeneous and dense gel network with high cross-linking degree (88.12 ± 1.80%) and water holding capacity (92.13 ± 2.40%), since pH 12-shifting and heating treatment promoted the conversion of random coils and β-turns to β-folds and α-helices in PPI gels. However, with pH 10 and 11-shifting, the PPI molecules was partially unfolded and the PPI gels had a high non-network protein content, resulting in low-strength gel networks of PPI gels with rough and loose microstructures and a low WHC. These results showed that TGase-crosslinked cold-set PPI gels after pH 12-shifting and heat treatment could promote the formation of homogeneous and dense gel network structure, so that improved the PPI gelation properties, which had great potential to be used as gelling agents or nutraceutical delivery systems for thermal sensitive bioactive compounds in food and non-food fields.

Therapeutic Potential of Nanomaterial-based Drug Delivery

The medical market for sophisticated therapeutic medicine delivery systems is growing at a breakneck pace. There are many benefits to using nanotechnology and nanomaterials in treating chronic human illnesses, such as the ability to target the delivery of drugs to particular areas. Improving the therapeutic effectiveness of current and future medications might be as simple as creating new drug delivery methods. The prime applications related to nanomaterial drug delivery include immunotherapeutic agents, diagnostic testing, cancer therapy and nutraceutical delivery. Nanotechnology has the capacity to collaborate with physics, biologists, chemists and pharmaceutics to form a multidisciplinary contribution in the rise of novel diagnostic and therapeutic technology. Nanomaterials can be used in specific sites targeted by drugs for brain illnesses. These nanodrugs can cause lesser immune response and maintain low inflammatory conditions compared to heavy drug particles. Along with this, nanodrugs can cause damage at the genetic level, create oxidative stress and inhibit cell death according to their particle size, shape, surface and its composition. In this current review various applications, current trends and recent developments in nanomaterials are elucidated. The detailed mechanism and cutting edge nanomaterial based drug delivery were explained along with this.

Hydrocolloid-based nutraceutical delivery systems: Potential of κ-carrageenan hydrogel beads for sustained release of curcumin

This study investigates the potential of κ-carrageenan hydrogel beads as a delivery system for curcumin, a bioactive compound with various health benefits. Hydrogel beads were prepared using the extrusion technique with a hypodermic needle. The encapsulation efficiency of curcumin in the κ-carrageenan hydrogel beads was found to be 74.61 ± 3.2 %. FTIR spectroscopy analysis revealed shifts in absorption peaks, indicating possible hydrogen bonding and/or ionic interactions between the polymer and salt. An increase in the melting point of curcumin, by 25 °C, in curcumin- κ-carrageenan beads suggests the heat protection offered by the carrageenan chains to curcumin molecules. The in vitro release of curcumin from the beads suggests a sustained and pH-dependent release nature. The release kinetics follow the first order and the Korsmeyer-Peppas model. The outcome offers value-added delivery systems of bioactive compounds toward developing novel food and pharmaceutical applications.

Whey protein isolate-stabilized gardenia fruit oil nanoemulsions: Ultrasonic preparation, characterization and applications in nutraceuticals delivery

In the current research, ultrasound was applied to make whey protein isolate (WPI)-gardenia fruit oil nanoemulsion. Effects of preparation conditions on physicochemical characteristics of nanoemulsions were investigated. The smallest droplet size was obtained at the oil content of 10% (v/v) and the WPI concentration of 0.5% (w/v) with ultrasound (2.4 W/mL, 12 min). Ultrasound led to a more uniform nanoemulsion with smaller droplet sizes (< 300 nm). The nanoemulsion prepared with ultrasound remained relatively stable for more than one week, while the crude emulsion prepared without ultrasound layered after 1 day storage. The nanoemulsion was more stable at lower temperature, alkaline condition and lower Na+ concentration. The formation of nanoemulsions significantly reduced the generation rate and amount of POV and TBARS during the 14-day accelerated oxidation experiment, increasing the oxidation stability of gardenia fruit oil. Additionally, the nanoemulsion could encapsulate β-carotene, hesperetin and naringenin at the efficiency of 79.61%, 51.29% and 46.06%, which further improved the oil oxidation stability and retarded free fatty acids release during the stimulated digestion in vitro.

Thermodynamic and network characteristics of optimized lysine-modified kutki (Panicum sumatrense) millet starch hydrogels.

Three-dimensional polymeric network of hydrogels avoids its dissolution into the aqueous region. Hydrogels must have strong structural integrity to be used for drug/nutraceutical delivery. A three factor-three-level Box-Behnken design was used to understand the effects of starch concentration, NaCl, and pH on the textural and structural integrity of Lysine modified kutki millet starch hydrogels. Various kinetic models were fitted to the time-course measurement of shrinkage behavior of both conventionally (CDHG) and freeze-dried (FDHG) hydrogel. Increasing the swelling temperature (5-50°C) showed values of higher molecular weight of polymer chains between neighboring crosslinks (g/mol) for FDHG (9539.59-56,769.72) than CDHG (1096.28-11,420.48). Similarly, mesh size (ξ) was more for FDHG (38.63-109.53 Ȧ) than CDHG (10.97-42.74 Ȧ). However, other network parameters such as polymer volume fraction ( ) was lower for FDHG (0.229-0.146) than CDHG (0.3882-0.222). These values suggest low swelling power of CDHG compared to FDHG. Thermodynamically, FDHG took less energy to swell than CDHG. The study showed that FDHG has better properties than CDHG and could be employed in nutraceutical delivery. The online version contains supplementary material available at 10.1007/s13197-024-05953-x.

Spontaneous nanoemulsification of cinnamon essential oil: Formulation, characterization, and antibacterial and antibiofilm activity against fish spoilage caused by Serratia rubidaea BFMO8.

The contemporary food industry's uses of nanoemulsions (NEs) include food processing, effective nutraceutical delivery, the development of functional chemicals, and the synthesis of natural preservatives, such as phytocompounds. Although cinnamon essential oil (CEO) is widely used in the cosmetic, pharmaceutical, and food industries, it is difficult to add to aqueous-based food formulations due to its weak stability and poor water solubility. This study describes the formulation of a CEO nanoemulsion (CEONE) by spontaneous emulsification and evaluates its antibacterial and antibiofilm properties against biofilm-forming Serratia rubidaea BFMO8 isolated from spoiled emperor fish (Lethrinus miniatus). Bacteria causing spoilage in emperor fish were isolated and identified as S. rubidaea using common morphological, cultural, and 16S RNA sequencing methods, and their ability to form biofilms and their susceptibility to CEONE were assessed using biofilm-specific methods. The spontaneous emulsification formulation of CEONE was accomplished using water and Tween 20 surfactant by manipulating organic and aqueous phase interface properties and controlling particle growth by capping surfactant increases. The best emulsification, with highly stable nano-size droplets, was accomplished at 750rpm and a 1:3 ratio concentration. The stable CEONE droplet size, polydispersity index, and zeta potential values were 204.8nm, 0.115, and -6.05mV, respectively. FTIR and high-resolution liquid chromatography-mass spectrometry (HR-LCMS) analyses have revealed carboxyl, carbonyl, and phenol-like primary phytochemical functional groups in CEO and CEONE, which contribute to their antibacterial and antibiofilm properties.