Objective To construct nurse′s training and assessment information system in level three general hospitals and apply it in clinical. Methods According to our hospital designed nursing training and assessment plan, medical nursing management information system divided itself into Nursing Department, Education Department, and Ward three grade nursing training management model, and the function maintenance and application of system were took by Nursing Department, Education Department, and Ward. All nursing staff members could choose the type of training according to the study plan and work requirement, and were assessed theory and skills once per half year. Before (December 2013) and after (December 2014) training management system application, Nursing Department investigated 468 nurses for training effects by the awareness of training information and satisfaction of training management. Results After the application of system, it improved the awareness of training information and training management satisfaction (χ2=31.26, 28.64; P<0.01). Conclusions Nursing training and assessment system effectively achieves annual training plan made by Nursing Department, and further strengthen nursing training management and nursing quality management. Key words: Nurses; Training; Assessment; Information management system; Satisfaction