Background: Enteral nutrition is a critical aspect of care for patients in intensive care units (ICUs) who cannot consume food orally. Understanding the practices and knowledge of ICU nurses regarding enteral nutrition is essential for ensuring effective patient care and outcomes. Objective: This study aimed to assess the knowledge and practices of ICU nurses regarding enteral nutrition and identify any gaps that could potentially impact patient care. Methods: A cross-sectional study was conducted at Jinnah Hospital, Lahore, using purposive sampling to recruit 142 ICU nurses. Data were collected over a nine-month period using a validated questionnaire designed to evaluate the nurses' knowledge and practices concerning enteral nutrition. Statistical analysis was performed using SPSS software version 25, focusing on descriptive and inferential statistics to understand the trends and associations within the data. Results: The study revealed that 65% of nurses correctly identified pancreatitis as an indication for starting enteral nutrition. However, 73% incorrectly believed that a continuous drip method of enteral feeding does not require a pump. Furthermore, 63% recognized tube dislodgement as a complication, yet 57% either did not know or incorrectly handled protocols for flushing enteral feeding tubes before use. The assessment of nasogastric tube placement every 24 hours was misunderstood by 57% of the participants. Conclusion: The findings indicate a considerable deficiency in the knowledge and practices of ICU nurses regarding enteral nutrition, with significant implications for patient care. There is a clear need for targeted educational programs and updated protocols to enhance the skills and understanding of nurses in this crucial area.