AbstractThis study investigates spreading and generation of spiciness anomalies of the Subantarctic Mode Water (SAMW) located on 26.6 to 26.8 σθ in the south Indian Ocean, using in situ hydrographic observations, satellite measurements, reanalysis datasets, and numerical model output. The amplitude of spiciness anomalies is about 0.03 psu or 0.13°C and tends to be large along the streamline of the subtropical gyre, whose upstream end is the outcrop region south of Australia. The speed of spreading is comparable to that of the mean current, and it takes about a decade for a spiciness anomaly in the outcrop region to spread into the interior up to Madagascar. In the outcrop region, interannual variability in mixed layer temperature and salinity tends to be density compensating, which indicates that Eulerian temperature or salinity changes account for the generation of isopycnal spiciness anomalies. It is known that wintertime temperature and salinity in the surface mixed layer determine the temperature and salinity relationship of a subducted water mass. Considering this, the mixed layer heat budget in the outcrop region is estimated based on the concept of effective mixed layer depth, the result of which shows the primary contribution from horizontal advection. The contributions from Ekman and geostrophic currents are comparable. Ekman flow advection is caused by zonal wind stress anomalies and the resulting meridional Ekman current anomalies, as is pointed out by a previous study. Geostrophic velocity is decomposed into large-scale and mesoscale variability, both of which significantly contribute to horizontal advection.