Patulin (PAT) contamination and changes of nonvolatile and volatile compounds caused by Penicillium expansum in fresh juices of 8 kiwi cultivars were investigated. Growth and PAT production of P. expansum were greater at 25°C than at 4°C. P. expansum grew in kiwi juices under pH ranging from 2.75 to 3.27 and produced 45.10-268.88μg/mL of PAT at 25°C. Decreases occurred in malic acid and soluble protein; while consumption and production simultaneously happened for reducing sugar, total soluble solid, titratable acid, citric acid, ascorbic acid and total phenolics. A large number of volatile organic compounds (VOCs) were produced during infection and each cultivar presented a distinct profile. Most of the alcohols, acids, ketones and phenols increased while aldehydes decreased. VOCs that were specific to infected kiwi juices are potential biomarkers for GC-MS analysis of kiwifruit infected by P. expansum.