Number Of Bacterial Cells Research Articles

ABILITY OF AMILOLYTIC BACTERIA (Bacillus paramycoides and Enterobacter cloacae) IN DEGRADING ORGANIC MATERIALS OF MANGROVE LITTLE

This study aims to find out that Bacillus paramycoides and Enterobacter cloacae bacteria can produce amylase enzymes and have the ability to degrade organic matter, especially mangrove litter. From this study it was found that the optimal growth of B.paramycoides and E. cloacae bacteria occurred at 12th hour. The results of measurements and calculations of absorbance values ​​at 630 10.238 x 108 cells/mL (B. paramycoides) and 12.030 x 108 cells/mL (E. cloacae) using the spectrophotometric method. Meanwhile, with the TPC method at 12 hours, the number of bacterial cells was 2.08 x 108 CFU's/ml (B. paramycoides) and 2.44 x 108 CFU's/ml (E. cloacae). The ability to produce the largest amylolytic bacterial amylase enzyme also occurred at 12 hours as much as 0.306 mg/mL (B.paramycoides) with an increase of 0.046 mg/mL and 0.243 mg/mL (E. cloacae) with an increase of 0.028 mg/mL. The bacteria that have the highest amylase enzyme ability is E.cloacae as evidenced by the diameter of the clear zone of 10.10 mm. Testing the ability of amylolytic bacteria in degrading mangrove litter was carried out by adding amylase enzyme as much as 0%, 50% and 100%. Amylolytic bacteria can degrade organic matter by hydrolyzing starch contained in mangrove litter. The most degraded starch content was in the 100% enzyme treatment, which was 1.021 mg/mL (B. paramycoides) and 1.189 mg/mL (E.cloacae).

Role of biofilms in the survival of Legionella pneumophila to sodium chloride treatment.

Background and Objectives:Legionnaires’ disease continues to be a public health concern. Colonized water distribution systems are often implicated in Legionella transmission, despite the use of various disinfection strategies, the bacterium is capable to persist and survive in water systems. The aim of this study was to investigate the persistence of Legionella pneumophila to sodium chloride over time at different temperatures and analysing the role of biofilms in the survival of this bacteria.Materials and Methods:L. pneumophila serogroup 1 and L. pneumophila serogroup 2–15 were used to study the effect of sodium chloride on planktonic and sessile cells. The tested concentrations were: 0.5%, 1%, 2%, 3%, 4%, 6% and 8% (W/V) NaCl. Biofilms were grown on 24-well microplates.Results:At 20°C, L. pneumophila planktonic cells were able to survive in sodium chloride concentrations up to 2%. However, at 37°C, a sodium chloride concentration over 1.5%, reduced systematically the numbers of bacterial cells. Biofilms were grown for 20 days in the absence and presence of sodium chloride. The results show that bacterial strains were able to survive and regrow after the sodium chloride shock (2–3%). Moreover, it seems that this effect is less expressed with the age of the biofilm; old biofilms were more persistent than the young ones.Conclusion:Results from this study demonstrate that the sodium chloride disinfection strategy was effective on Legionella pneumophila planktonic cells but not on biofilms, which demonstrate the role of biofilms in the persistence and recolonization of L. pneumophila in water distribution systems.

Antimicrobial Efficacy and Spectrum of Phosphorous-Fluorine Co-Doped TiO2 Nanoparticles on the Foodborne Pathogenic Bacteria Campylobacter jejuni, Salmonella Typhimurium, Enterohaemorrhagic E. coli, Yersinia enterocolitica, Shewanella putrefaciens, Listeria monocytogenes and Staphylococcus aureus.

Contamination of meats and meat products with foodborne pathogenic bacteria raises serious safety issues in the food industry. The antibacterial activities of phosphorous-fluorine co-doped TiO2 nanoparticles (PF-TiO2) were investigated against seven foodborne pathogenic bacteria: Campylobacter jejuni, Salmonella Typhimurium, Enterohaemorrhagic E. coli, Yersinia enterocolitica, Shewanella putrefaciens, Listeria monocytogenes and Staphylococcus aureus. PF-TiO2 NPs were synthesized hydrothermally at 250 °C for 1, 3, 6 or 12 h, and then tested at three different concentrations (500 μg/mL, 100 μg/mL, 20 μg/mL) for the inactivation of foodborne bacteria under UVA irradiation, daylight exposure or dark conditions. The antibacterial efficacies were compared after 30 min of exposure to light. Distinct differences in the antibacterial activities of the PF-TiO2 NPs, and the susceptibilities of tested foodborne pathogenic bacterium species were found. PF-TiO2/3 h and PF-TiO2/6 h showed the highest antibacterial activity by decreasing the living bacterial cell number from ~106 by ~5 log (L. monocytogenes), ~4 log (EHEC), ~3 log (Y. enterolcolitca, S. putrefaciens) and ~2.5 log (S. aureus), along with complete eradication of C. jejuni and S. Typhimurium. Efficacy of PF-TiO2/1 h and PF-TiO2/12 h NPs was lower, typically causing a ~2–4 log decrease in colony forming units depending on the tested bacterium while the effect of PF-TiO2/0 h was comparable to P25 TiO2, a commercial TiO2 with high photocatalytic activity. Our results show that PF-co-doping of TiO2 NPs enhanced the antibacterial action against foodborne pathogenic bacteria and are potential candidates for use in the food industry as active surface components, potentially contributing to the production of meats that are safe for consumption.

The Effect of the Addition of Chokeberry Fiber on the Quality of Sheep's Milk Fermented by Lactobacillus rhamnosus and Lactobacillus acidophilus.

The global market for sheep's milk and its products is increasing due to higher demand for cheese and traditional dairy products, and as a novelty, sheep's milk is an ingredient in infant formulas and nutraceuticals. The aim of this study was to determine the properties of fermented sheep's milk, which combines probiotic benefits with increased dietary fiber content. The influence of the applied dose of chokeberry fiber on the growth of living cells of Lactobacillus acidophilus and Lactobacillus rhamnosus in fermented sheep's milk was also evaluated. Sheep milk with the addition of 0% (control sample), 1.5%, and 3.0% chokeberry fiber was fermented by two different probiotic monocultures L. acidophilus and L. rhamnosus. In the fermented milk, pH value, syneresis (%), color, and texture were determined. Furthermore, the microbiological analysis and an organoleptic evaluation were performed. With the increasing dose of chokeberry fiber, the pH value decreased already before the fermentation process. After fermentation, the milk's pH values with fiber were still lower than those in the control sample. Presumably, more acidic metabolites were produced by L. acidophilus, causing a more intense reduction of the pH value than L. rhamnosus both in control milk and in milk with the addition of fiber. The addition of chokeberry fiber affected the stimulation of the growth of both types of bacteria. In the milk sample without fiber addition, a more significant number of viable cells were counted for L. acidophilus by 0.5 log CFU g−1 more than the milk fermented with L. rhamnosus. Furthermore, in milk fermented by L. acidophilus with 1.5% chokeberry fiber (LA1), the number of viable bacterial cells was higher than that in milk fermented by L. rhamnosus with the same addition of fiber (LR1). However, in sample LA3, the number of viable bacterial cells was lower than that in sample LR3. Tested fermented sheep milk met the Recommendation of the International Dairy Federation's requirements regarding the number of live bacterial cells for dairy probiotics. The addition of chokeberry fiber increased syneresis in each fermented milk group, regardless of the bacteria used for fermentation. Moreover, the use of fiber caused a significant reduction in brightness L∗, an increase of red color, and a decrease of yellow color. Milk fermented with L. acidophilus was characterized by a harder gel, compared to their analogues fermented with L. rhamnosus. The addition of fiber intensified the sour taste and the taste of the additive in both types of fermented sheep milk.

Formation of biofilms by representatives of the oral microflora on the surfaces of basic materials

The aim of the work is to conduct a comparative analysis of the biofilm formation by representatives of the oral microflora on the surfaces of basic materials. Materials and methods. The process of biofilm formation was examined on 7 types of basic plastic samples: Polyan, Breflex, Nylon, Protakryl, Vinakryl, Biocryl, which were used for the manufacture of removable prosthetic basis constructions, and SYNMA, which was used for comparison. Biofilm formation was analyzed by the method Y. Zhang (2017) with minor modifications. The test sample was placed in a test tube with 2.0 ml of nutrient broth Brain Heart Infusion to model the biofilm growth of microorganisms (HiMedia Laboratories Pvt. Ltd., India) supplemented with 1 % glucose, pre-inoculated with test strains at a final concentration of 1 × 104 CFU/ml. The strains were cultivated for 24 hours at a temperature of 37 °C under continuous stirring in a shaker MR-1 (SIA BIOS AN, Latvia) at 20 rpm. Evaluation of the biofilm massiveness was performed after gentian violet staining followed by elution of the stain with ethanol and registration of the eluent optical density (OD). The OD was measured with a Synergy™ HTX S1LFTA microplate multimode photometer (BioTek Instruments, Inc., USA) at 595 nm wavelength using Gen5™ Data Analysis Software. The number of viable bacterial cells in the formed biofilms was determined by the method of ten-fold serial dilutions. The obtained results were converted per unit area of the sample tested. Processing of the results was performed using a two-sample t-test with the software package Statistica 13.0 and Microsoft Office Excel, the differences were considered statistically significant at a P value of < 0.05. Statistical analysis of the obtained data was presented as mean values of measurements ± standard deviation for three independent experiments. Results. According to the microbiological analysis results it was found that α-hemolytic streptococci S. oralis and S. sanguinis showed the ability to form biofilms on the surfaces of basic materials, namely Protacryl and Vinacryl, the total biomass of S. sanguinis biofilms was 47.7 % (P < 0.01) and 14.7 % (P > 0.05) greater, respectively, in comparison to a glass slide. Inhibition of biofilm formation processes was observed on the surfaces of Nylon and Biocryl basic materials. S. oralis and S. gordonii showed the highest ability to survive in biofilms. The intensity of C. albicans biofilms formation on Biocryl basic materials, comparative plastics SINMA and Breflex basic materials was greater than on glass slides by 48.3 %, 43.0 % and 34.9 % (P < 0.01), respectively. The least massive C. albicans biofilms were formed on Breflex surfaces and SINMA comparative plastics in comparison to glass slides by 33.6 % and 24.8 % (P < 0.01), respectively. Both Candida strains had the highest level of fungal viability in biofilms on Breflex, Polyan and Protacryl basic materials (P < 0.01), and C. tropicalis biofilms on Biocryl and Vinacryl basic materials (P < 0.05). Integral coefficients indicated the inhibition of the oral microflora ability to form biofilms on the surfaces of basic materials. Conclusions. Oral α-hemolytic and β-hemolytic streptococci have the ability to intensive biofilm growth on the surfaces of the basic materials Protacryl and Vinacryl. Oral Candida albicans form massive biofilms on the surfaces of Biocryl and Vinacryl basic materials and comparative SYNMA plastics. The basic materials Breflex, Nylon and comparative plastics SYNMA are the most inert to biofilm formation by the oral microflora representatives.

DIAZOTROPH ACTIVITY REGULATING STRATEGY UNDER THEIR INTRODUCTION IN AGROCENOSES

Objective. Investigate approaches to managing the activity of soil diazotrophs and propose a strategy for its regulation. Methods. Theoretical, vegetation and field experiments, microbiological, gas chromatographic, mathematical and statistical. Results. The activity of beneficial soil microorganisms can change under the action of temperature, humidity, chemical compounds of various origin, and other microorganisms. It was established that, taking into account a significant variety of factors, it is necessary to develop a set of specific ways to increase the growth and functional activity of nitrogen-fixing bacteria, as well as their viability. It has been proved that the combination of diazotrophs forms an effective symbiotic leguminous-rhizobial system, which provides additional biological nitrogen in agrocenoses. At the same time, there was an increase in plant mass, chlorophyll content in the leaves, protein and oil content in the products. The combined use of diazotrophs increases the yield, in particular, soybeans by 9–16 % compared with inoculation by pure bacterial culture. Conclusion. Based on the analysis and generalization of the obtained research results, a strategy for regulating the activity of diazotrophs for their effective introduction into agrocenoses is proposed, which consists in combining bacteria of different species, selecting conditions for their co-cultivation and application upon stabilisation of the number of viable bacterial cells. The proposed strategy involves solving the problem by obtaining an inoculant, which is characterized by a high titre and a stable number of viable cells, which allows to obtain an effective nitrogen-fixing system. The strategy is tried-and-tested on the example of regulating the growth and functional activity of soybean nodule bacteria by combining diazotrophs of different species, substantiating the conditions of their co-cultivation and application to ensure positive interaction in the form of commensalism, as well as by regulating viability of diazotrophs by adding stabilisers to the medium.

Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60

Introduction. In recent years, due to wide applications of nanotechnologies in various fields, the safety of nanomaterials has become a pressing issue. Fullerene C60 is not an exception. Research on the activity of microorganisms and their interaction with nanoparticles is of major importance, both for microorganisms and for the ecosystem as a whole.
 Material and methods. Fullerene C60 powder was purchased from Sigma-Aldrich. The object of study was R. rhodochrous CNMN-Ac-05 strain. The number of viable bacterial cells was estimated by colony-forming units (CFU). The morphological features of the rhodococci colonies have been described according to the usual microbiological method.
 Results. It was established that fullerene C60 in concentrations of 1-25 mg/L fullerene C60 stimulated the growth of R. rhodochrous by 2.4-2.8 times. As the concentration of fullerene C60 increased up to 50-100 mg/L, the multiplication and growth of rhodococci decreased by 29.5% and 38% respectively. In the presence of 1-10 mg/L fullerene C60 the rhodococci population remained homogeneous, being composed of 100% S type colonies. The increase of fullerene C60 concentration led both to the decrease in the CFU number and to the appearance of R type colonies, up to 1.3% of population.
 Conclusions. Fullerene C60 in concentrations 1-100 mg/L had no obvious toxic effect on the rhodococci strain. The optimum concentration is 10 mg/L. The concentrations higher than 25 mg/L led to the dissociation of rhodococcal population and diminution in the CFU counts, but not to the total inhibition.

Combined endodontic treatment: microbiological aspects by using scanning electronical microscopy

Aim. To determine possible alternative algorithms for root canal disinfection through the combined use of chemical and physiotherapeutic irrigation techniques under in vitro conditions.Materials and methods. Single-root teeth removed according to orthodontic indications were used as a substrate for the formation of a biofilm. The formation of a mixed biofilm is carried out in several stages, successively introducing the following strains: Streptococcus sanguinis; Fusobacterium nucleatum; Porphyromonas gingivalis. Cultivation was carried out under fluid conditions under anaerobic conditions, for 168 hours. After the cultivation, the teeth were treated using: a solution of chlorhexidine bigluconate 2%; a solution of sodium hypochlorite 3%; photodynamic exposure with Fotoditazin photosensitizer; 1.0% sodium hypochlorite solution in combination with photodynamic exposure. The results were evaluated using scanning electron microscopy.Results. The results of scanning electron microscopy revealed differences between the samples of the control group and the comparison group. The number of bacterial cells was significantly lower in the comparison group than in the control group, however, in all samples, the presence of microbial biofilm sites was noted with varying degrees of severity. In the samples where standard concentrations were used, there was a change in the ultrastructure of the walls of the dentine tubules with its partial destruction and obturation by its own fragments of the walls with the presence of microbial cells. When using the photodynamic effect of destruction was not observed. The microbial landscape had a similar species composition, and more fragments of biofilm and individual cells were observed only in the case of PDT without a combination with an antiseptic.Conclusions. A comprehensive approach to endodontic treatment using photodynamic therapy together with antiseptic solutions in low concentrations allows us to achieve the best effect of pathogen eradication in comparison with the standard chemomechanical method.

PASSAGE OF WHEAT FIRE IMPACT ON SOIL PROPERTIES AND ENVIRONMENT IN KURDISTAN REGION

The study was conducted to examine the effect of surface burn severity (Moderate, Severe and Unburned) of wheat straw on soil properties. The results showed statistical differences in some soil physical, chemical and biological properties. Bulk density and field capacity increased statistically by the severity of fire; however, porosity and infiltration rate were statistically lower in sever burned plot when compared to unburned plot. The chemical properties, soil organic matter (SOM), P, Ca, S, Cl, K, Mo, Fe and As were not affected by the fire. The pH value was increased slightly by increasing the fire severity, while, EC was decreased when compared with the unburned plot. It was found a statistical reduction in the number of bacterial and fungal cells per gram soil in the burned plots. A moderate and severe fire reduced seed germination percentage significantly. This finding suggests that fire severity may destruct the biological, physical and some of the chemical properties of the soil, and this may impact negatively on plant growth in the next growing season.

\u201cLimiting access to iron decreases infection of Atlantic salmon SHK-1 cells with bacterium Piscirickettsia salmonis\u201d

BackgroundVertebrate hosts limit the availability of iron to microbial pathogens in order to nutritionally starve the invaders. The impact of iron deficiency induced by the iron chelator deferoxamine mesylate (DFO) was investigated in Atlantic salmon SHK-1 cells infected with the facultative intracellular bacterium Piscirickettsia salmonis.ResultsEffects of the DFO treatment and P. salmonis on SHK-1 cells were gaged by assessing cytopathic effects, bacterial load and activity, and gene expression profiles of eight immune biomarkers at 4- and 7-days post infection (dpi) in the control group, groups receiving single treatments (DFO or P. salmonis) and their combination. The chelator appears to be well-tolerated by host cells, while it had a negative impact on the number of bacterial cells and associated cytotoxicity. DFO alone had minor effects on gene expression of SHK-1 cells, including an early activation of IL-1β at 4 dpi. In contrast to few moderate changes induced by single treatments (either infection or chelator), most genes had highest upregulation in the infected groups receiving DFO. The mildest induction of hepcidin-1 (antimicrobial peptide precursor and regulator of iron homeostasis) was observed in cells exposed to DFO alone, followed by P. salmonis infected cells while the addition of DFO to infected cells further increased the mRNA abundance of this gene. Transcripts encoding TNF-α (immune signaling) and iNOS (immune effector) showed sustained increase at both time points in this group while cathelicidin-1 (immune effector) and IL-8 (immune signaling) were upregulated at 7 dpi. The stimulation of protective gene responses seen in infected cultures supplemented with DFO coincided with the reduction of bacterial load and activity (judged by the expression of P. salmonis 16S rRNA), and damage to cultured host cells.ConclusionThe absence of immune gene activation under normal iron conditions suggests modulation of host responses by P. salmonis. The negative effect of iron deficiency on bacteria likely allowed host cells to respond in a more protective manner to the infection, further decreasing its progression. Presented findings encourage in vivo exploration of iron chelators as a promising strategy against piscirickettsiosis.

Targeting Candida albicans in dual-species biofilms with antifungal treatment reduces Staphylococcus aureus and MRSA in vitro.

Polymicrobial biofilms consisting of fungi and bacteria are frequently formed on endotracheal tubes and may contribute to development of ventilator associated pneumonia (VAP) in critically ill patients. This study aimed to determine the role of early Candida albicans biofilms in supporting dual-species (dual-kingdom) biofilm formation with respiratory pathogens in vitro, and investigated the effect of targeted antifungal treatment on bacterial cells within the biofilms. Dual-species biofilm formation between C. albicans and three respiratory pathogens commonly associated with VAP (Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus) was studied using quantitative PCR. It was shown that early C. albicans biofilms enhanced the numbers of E. coli and S. aureus (including methicillin resistant S. aureus; MRSA) but not P. aeruginosa within dual-species biofilms. Transwell assays demonstrated that contact with C. albicans was required for the increased bacterial cell numbers observed. Total Internal Reflection Fluorescence microscopy showed that both wild type and hyphal-deficient C. albicans provided a scaffold for initial bacterial adhesion in dual species biofilms. qPCR results suggested that further maturation of the dual-species biofilm significantly increased bacterial cell numbers, except in the case of E.coli with hyphal-deficient C. albicans (Ca_gcn5Δ/Δ). A targeted preventative approach with liposomal amphotericin (AmBisome®) resulted in significantly decreased numbers of S. aureus in dual-species biofilms, as determined by propidium monoazide-modified qPCR. Similar results were observed when dual-species biofilms consisting of clinical isolates of C. albicans and MRSA were treated with liposomal amphotericin. However, reductions in E. coli numbers were not observed following liposomal amphotericin treatment. We conclude that early C. albicans biofilms have a key supporting role in dual-species biofilms by enhancing bacterial cell numbers during biofilm maturation. In the setting of increasing antibiotic resistance, an important and unexpected consequence of antifungal treatment of dual-species biofilms, is the additional benefit of decreased growth of multi-drug resistant bacteria such as MRSA, which could represent a novel future preventive strategy.

CONCENTRATION OF WATER POLLUTION INDICATORS BACTERIA IN SIRIH PADANG SUMATERA BARAT

This research was conducted in October 2019 in the waters of Sirih Bay Padang, West Sumatra Province. The objective of this research aimed was to determine the number of bacterial cells as an indicator of water pollution (total coliform and Escherichia coli) in the waters of Sirih Bay, Padang. The method used in this study was a survey method. Polluted waters in Sirih Bay as sampling location was divided into 3 stations, namely the PLTU area (St. I), near BPBALP Padang (St. II) and a residential area (St. III). Isolation of total coliform and fecal coliform bacteria was carried out by assumption and affirmation tests which were further identified and carried out physiological and biochemical properties. The results showed the total number of coliforms in Sirih Bay waters in the assertion test using BGLB media ranged from 75-460 MPN/ml. This indicates the existence of pollution of coliform bacteria in these waters based on the Decree of the State Minister of Environment No. 51 of 2004 concerning Sea Water Quality Standards, the maximum level of total coliform bacteria is 1000 MPN / 100 ml. The results of this study indicated that the waters of Sirih Bay are considered to be in poor condition

Integration of pre-colonized and mediator immobilized mixed culture for the improvement of electricity production of microbial fuel cells

In this study, we have immobilized bacterial cells with mediators as bioaugmentation to increase the numbers of bacterial cells in anodic biofilm for better microbial fuel cell (MFC) performance. Four individual bacterial strains (strain E1, strain SCS5, strain B2 and Shewanella putrefaciens) and their mixed culture were first evaluated for electricity production in MFCs. The mixed culture MFC produced higher power density (3.021 Wm−3) than pure strains. The pre-colonized (PC) biofilms together with mediator immobilized mixed culture (MIMC) beads as bioanodes further enhanced the performance of MFCs. Integrated PC/MIMC MFCs containing anthraquinine-2,6-disulfonate (AQDS) and Fe3O4 immobilized mixed culture beads produced 1.50 and 1.47 times power density with respect to mixed culture biofilm, respectively. AQDS immobilization showed better performance compared to Fe3O4 in energy output. The integrated use of PC biofilms and MIMC exhibited lower charge transfer resistance than corresponding individual anode biofilms in MFCs according to electrochemical impedance spectroscopy analysis. The MFC having PC/MIMC as anode showed the ability to store electrons generated by bacteria, but the biocapacitance of anode was altered after integrating MIMC bead particles. Thus, the collective use of PC biofilm and MIMC beads will be one of the potential choices for improving MFC performance.

Olive mill wastewater effects on durum wheat crop attributes and soil microbial activities: A pilot study in Syria

Olive mill wastewater is one of the environmental problems in semiarid regions. The main goals of this study were to investigate the impacts of different olive mill wastewater levels on durum wheat (Triticum aestivum var. Douma1) production and soil microbial activities (i.e., bacteria and fungi). A pot experiment was conducted during the growing seasons 2015/2017 to evaluate the effect of three levels of olive mill wastewater on both growth and productivity attributes of wheat. Vertisol soil samples were collected from southern Syria. Two months before wheat cultivation, three levels of olive mill wastewater: T5 (5 L/m 2), T10 (10 L/m2) and T15 (15 L/m 2) were added to pots filled with the collected soil samples. Also, a control (T0) free of olive mill wastewater was considered as a reference. Results showed a significant increase (p < 0.05) in germination rate (%), plant height (cm), ear length (cm), kernels number, kernels weight per ear (g) and grain yield (g/m2) compared to control. However, T5 treatment did not induce a significant increase in terms of ear length, kernels weight per ear or yield (in the second season). On the other hand, T10 treatment had recorded the best results compared with the other two treatments (T5, T15). Similarly, the results showed a significant increase in the number of bacterial and fungi cells by increasing olive mill wastewater concentration. This research provides promising results toward using olive mill wastewater in an eco-friendly way under Syrian conditions.

Comparison of three qPCR-based commercial tests for detection of periodontal pathogens

In periodontal practice microbial results of periodontal test kits for identification of key pathogens are an aid in the treatment planning. Information on the performance of commercially available test kits is therefore essential for the clinician. In this retrospective analysis three commercially available qPCR kits for detection and quantification of selected periodontal bacterial species were compared, using 100 clinical samples from patients with untreated periodontitis. The analysis involved two separate comparisons in which kit A (LabOral Diagnostics, The Netherlands) was compared with kit B (Advanced Dental Diagnostics, The Netherlands), and with kit C (OralDent diagnostics, The Netherlands). Analytic procedures for detection and quantification of selected periodontal bacterial species were carried out according to the instructions of the laboratories. Kit A detected target species more often, and absolute numbers of bacterial cells were higher than with kit B. A high degree of similarity was found between the test outcomes by kit A and kit C. All three kits performed satisfactory but small and significant differences exist between kits.

Bank on Microbiome to keep the Body Healthy

Generally our human body contains large number of bacteria, viruses and fungi and they are collectively known as the microbiome community. While some bacteria are associated with disease, others are actually very important for strengthening of our immune system, proper functioning of the body organs including heart, maintenance of body weight and many other aspects of health. Trillions of these microbes exist in our body system particularly in the intestines and skin. Most of the microbes are associated in the large intestine in the form of a pocket called the cecum, and they are referred to as the gut microbiome. Although many different types of microbes are present in our body, however most of the studies have been carried on bacterial composition of the digestive gut system. In fact, there are several reports that the human body contains more number of bacterial cells than the actual human cell counts. It has been estimated that roughly 40 trillion bacterial cells are present in our body whereas human cells counts are around 30 trillion. Scientists all over the world have reported about 1,000 species of bacteria in the human gut microbiome, and each of them plays a different role in the body. Most of them are extremely important for maintaining our body health, while few species may be harmful in creating some serious diseases. Altogether, these microbes may weigh as much as 2-5 pounds (1-2 kg), which is roughly the weight of our brain.

Cobalt-Chromium Alloy Has Superior Antibacterial Effect Than Titanium Alloy: In Vitro and In Vivo Studies.

In vitro and in vivo laboratory studies. This study aimed to compare bacterial survival on titanium alloy (Ti) and cobalt-chromium alloy (CC) using in vitro and in vivo experiments. Spinal implants are frequently manufactured from Ti and CC. These foreign materials are thought to be susceptible to biofilm formation that contributes to the development of surgical site infections. Certain metals (i.e., silver, cobalt) are known to have antibacterial properties. In the in vitro study, discs made of Ti or CC were incubated with one of two common bacteria: Staphylococcus aureus (S. aureus) and Propionibacterium acnes (P. acnes). After incubation, discs were assessed to determine the number of viable bacterial cells. In the in vivo study, the discs that were made of CC or Ti were implanted into the subcutaneous layer of BALB/c mice. After skin closure, a suspension including either S. aureus or P. acnes was directly inoculated on the implanted discs. The discs were retrieved and analyzed to determine the number of viable bacteria at 0.5, 1, and 3 days after inoculation. The number of viable S. aureus cultured from the CC discs was 0.9 ± 0.2 × 103 CFU/disc, which was significantly lower than the cultured Ti discs (114.8 ± 18.3 × 103 CFU/disc). Moreover, a significantly lower mean number of P. acnes were cultured with CC (1.9 ± 1.2 × 103 CFU/disc) compared with the Ti (180.0 ± 72.1 × 103 CFU/disc). The in vivo infection model testing against S. aureus or P. acnes showed a significantly lower number of viable S. aureus or P. acnes on CC discs than Ti discs. The result was seen at all measured time points. CC suppressed S. aureus and P. acnes proliferation compared with Ti in vitro and in an in vivo infection model.Level of Evidence: N/A.

Bacterial and abiogenic carbonates formed in caves-no vital effect on clumped isotope compositions.

Speleothems (dominated by cave-hosted carbonate deposits) are valuable archives of paleoclimate conditions. As such, they are potential targets of clumped isotope analyses that may yield quantified data about past temperature variations. Clumped isotope analyses of stalagmites, however, seldom provide useful temperature values due to various isotope fractionation processes. This study focuses on the determination of the microbially induced vital effect, i.e., the isotope fractionation processes related to bacterial carbonate production. A cave site with biologically mediated amorphous calcium carbonate precitation was selected as a natural laboratory. Calcite deposits were farmed under a UV lamp to prevent bacterial activity, as well as under control conditions. Microbiological analyses and morphological investigations using scanning electron microscopy showed that the UV lamp treatment effectively reduced the number of bacterial cells, and that bacterial carbonate production strongly influenced the carbonate’s morphology. Stable oxygen isotope analyses of calcite and drip waters, as well as clumped isotope measurements revealed that, although most of the studied carbonates formed close to oxygen isotope equilibrium, clumped isotope Δ47 values varied widely from equilibrium to strongly fractionated data. Site-specific kinetic fractionations played a dominant role in the distribution of Δ47 values, whereas bacterial carbonate production did not result in a detectable clumped isotope effect.

Evaluation of The Effects of Milking Hygiene and Sanitation Education on Total Bacterial and Somatic Cell Number of Bulk Tank Milk in Dairy Cattle Breeding

Hygiene and sanitation, which are of great importance for the protection of human. Beside this quality and quantity of the milk is very important for human health. The aim of this project is to develop a training model in raw milk hygiene in Aydın province, and to increase knowledge, skills and competencies of vocational/professional groups in raw milk production. Two surveys were conducted for the farm workers to be trained within the scope of the project. Firstly, a demonstrative survey was conducted for the farms where milk hygiene training was provided. The education satisfaction survey was conducted for the people who were educated after the seminar. The surveys were prepared by the project executives and the questions were answered by face to face interview method. The milk samples of the study were collected from 157 dairy farm bulk tanks located in Aydin province, before the beginning of the project and after training courses. The milk samples were analyzed for Total Viable Count (TVC) and Somatic Cell Count (SCC). In conclusion, 11% of tank milk samples were below the norm value of 100000cfu/mL out of 157 farms. It is also seen that 27 farms reduced their SCC values below 400000cells/mL out of 56 farms with a norm value of ≥400000cells/mL after hygiene training. When the results were evaluated in general, hygiene was improved by 11% in average TVC value and 1% in SCC value after milking hygiene education.

Sampling challenges in diagnosis of chronic bacterial infections.

In recent decades there has been an increase in knowledge of the distribution, species diversity and growth patterns of bacteria in human chronic infections. This has challenged standard diagnostic methods, which have undergone a development to both increase the accuracy of testing as well as to decrease the occurrence of contamination. In particular, the introduction of new technologies based on molecular techniques into the clinical diagnostic process has increased detection and identification of infectious pathogens. Sampling is the first step in the diagnostic process, making it crucial for obtaining a successful outcome. However, sampling methods have not developed at the same speed as molecular identification. The heterogeneous distribution and potentially small number of pathogenic bacterial cells in chronic infected tissue makes sampling a complicated task, and samples must be collected judiciously and handled with care. Clinical sampling is a step in the diagnostic process that may benefit from innovative methods based on current knowledge of bacteria present in chronic infections. In the present review, we describe and discuss different aspects that complicate sampling of chronic infections. The purpose is to survey representative scientific work investigating the presence and distribution of bacteria in chronic infections in relation to various clinical sampling methods.