Number Of Siliques Research Articles

Foliar application of silver (Ag-NPs) and copper (Cu-NPs) nanoparticles enhances phenotypic traits and oil quality in Brassica napus L.

Nanotechnology represents a burgeoning field of science that enables advanced research across various domains. In recent years, there has been a notable increase in the utilization of silver nanoparticles (AgNPs) for diverse agricultural and industrial applications. Similarly, copper nanoparticles (CuNPs) have significant attention in agriculture due to their cost-effectiveness and practicality. This study aimed to investigate the potential impact of silver and copper nanoparticles on enhancing both the yield and quality of Brassica napus L. cultivar CON-1. The combined foliar application of green synthesized silver and copper nanoparticles at varying concentrations (0, 5, 10, 15, 20, 25, 30, and 35mg/L) resulted in notable improvements in the growth, yield and quality of Brassica napus L. compared to untreated plants. Particularly, the concentration of 25mg/L for both silver and copper nanoparticles demonstrated superior outcomes in terms of plant height, number of primary and secondary branches/plant, number of siliques /plant, number of seeds/silique, siliqua length, 1000-seed weight, biological yield/plant, seed yield/plant, oil yield/plant, and harvest index. Furthermore, the nanoparticles effectively reduced the accumulation of erucic acid in the oil, thereby enhancing the overall oil quality of canola. In summary, the application of silver and copper nanoparticles shows promising potential in improving the productivity and quality of Brassica napus L., highlighting their role as beneficial agricultural additives in modern farming practices.

Deciphering the Arf (ADP-ribosylation factor) gene family in Brassica napus L.: Genome-wide insights into duplication, expression, and rapeseed yield enhancement

The Arf gene family is essential for crop growth and development by regulating vesicle transport. However, few studies exist on the role of Arfs in the growth and yield formation of Brassica napus. Here we provide an exhaustive account of the phylogeny and expression of the 66 Arfs in rapeseed. We found that the expansion of Arf gene family is mainly through whole genome duplication, and some genes are loss during the expansion process. Expression analysis revealed that the Arfs in group X, with the exception of BnaC02.ARFA1B, BnaC06.ARFA1A.2, and BnaA07.ARFA1A.2, exhibited high expression levels across various tissues of B. napus at different developmental stages. These results indicate that the Arfss in group X were important in influencing rapeseed growth and development. We have found that Arfs in B. napus may have a more complex regulatory mechanism due to homologous recombination and gene sub-functionalization. Haplotype analysis indicated that Arfs regulate B. napus yield formation. We found high expression of BnaC07.ARFA1A in all tissues, and its overexpression significantly increased rapeseed silique number and yield. The comprehensive analysis will further characterize the functions of Arfs in B. napus and enhance regulatory networks for yield formation in B. napus.

Growth and Yield Performance of Different Mustard Varieties Grown in Barind Tract Soils of Bangladesh

The present research was conducted to investigate the growth and yield performance of thirteen mustard varieties grown in Chapai Nawabganj district, Bangladesh. Thirteen mustard varieties, viz. “BINA Sarisha-4”, “BINA Sarisha-7”, “BINA Sarisha-9”, “BINA Sarisha-10”, “BARI Sarisha-14”, “BARI Sarisha-15”, “BARI Sarisha-16”, “BARI Sarisha-17”, “BARI Sarisha-18”, an “Indian tall” variety, “Indian short” variety, a Japanese variety “Sarisha” and a local variety were cultivated during “Rabi” (rainy) season 2021-2022. The preparation of land with recommended fertilizer doses, the experiment was arranged in a randomized complete block design consisting of three replications. All the growth, yield, and yield attributes significantly differed among the mustard varieties. In growth attributes, the highest value in plant height (149.97 cm), number of leaves per plant (16.77), leaf length (12.33 cm), number of branches per plant (11.40), and tap root length (22.53 cm) for “Indian tall” variety, “Indian short” variety , “BARI Sarisha-18”, “BINA Sarisha-7”, and “Japanese Sarisha”, respectively. In terms of yield and yield attributes, number of siliqua per plant (102.00), siliqua length (6.37 cm), number of grains per siliqua (39.50), grain yield per plot (576.33), 1000-seeds weight (3.75 g), biological yield (5.06 ton.ha-1), and harvest index (35.23%) was highest in the “Indian tall” variety , “BARI Sarisha-14”, “BARI Sarisha-14”, “BINA Sarisha-9”, and “Indian short” variety , respectively. The maximum benefit cost ratio (BCR) was 2.21, observed in “BINA Sarisha-9”. Besides, BCR 1.71 and 1.66 were for “BARI Sarisha-14” and “Japanese Sarisha”, respectively. The findings of the study suggested that “BINA Sarisha-9”, “BARI Sarisha-14”, and “Japanese Sarisha” would be suitable for better productivity and recommended for cultivation in the medium highland of Chapai Nawabganj district of Bangladesh.

A comparative study of yield components and their trade-off in oilseed crops (Brassica napus L. and Brassica carinata A. Braun)

Canola (Brassica napus L.) and carinata (Brassica carinata A. Braun) are two oilseed crops that have seen a surge in demand in recent years. However, current yields achieved by farmers are inconsistent and significantly lower than those achieved in research experiments. Therefore, a better understanding of the trade-off between yield components is crucial to help breeders develop new high-yielding cultivars and for farmers to manage their crops more effectively. Unfortunately, scientific literature focused on trade-offs between yield components in rapeseed is scarce for canola and lacking for carinata. To address this issue, we conducted field experiments in Uruguay over three consecutive seasons (2016–2018) using the cultivars “Rivette” and “Avanza 641” for canola and carinata, respectively. Our goal was to study yield compensation and determine the comparative performance of canola and carinata across a wide range of sowing dates. Our results showed that carinata yields ranged from 854 kg ha−1 to 7283 kg ha−1, while canola yields ranged from 1152 kg ha−1 to 4489 kg ha−1. Higher biomass production was linked to increased yields in carinata, while an increase in biomass did not necessarily result in higher yields in canola due to a harvest index reduction. Carinata yield was positively correlated with grain number, silique number, and grains per silique. In canola, moderate to large yield changes were primarily influenced by grain number and grain weight, as silique number had a negative trade-off with yield, while grains per silique had a neutral relationship with yield. Our study revealed a distinct yield building process in canola and carinata, despite the general belief that they share physiological similarities and can be managed alike. These findings enhance our understanding of crop knowledge and management, potentially leading to increased crop productivity, and generate more effective breeding programs. Enhancing the yield performance of canola and carinata will position them as economically profitable crops, allowing for greater crop diversification and meeting the growing global demand for biofuels.

Stability analysis on elite genotypes of Indian Mustard (Brassica Juncea L.) in Terai Agro-Climatic Region

Abstract Among the oilseed crops, mustard has gained precedence in the human diet due to its superior oil quality. Genotype × environment interactions have a significant effect on plant breeding because it reduces the stability of the genotypes in diverse environments. In order to assess the impact of environments on genotypes and determine the stable genotypes in terms of seed yield and yield attributing characters in the terai agro-climatic zone, seventy-one Indian mustard genotypes were evaluated during two consecutive years,namely, 2017-18 and 2018-19, focusing on nine quantitative characters across six environments. Eberhart and Russell model (1966) of stability analysis revealed that various genotypes exhibited adaptability to favourable and unfavourable environments for different characters, influenced by the different sowing dates over the two years. However, Rohini (SC) and KMR-15-14 to be well adapted in terai agro-climatic zone in respect to seed yield and plant height, while Pusa mustard 27 (EJ 17) were adapted to specifically favorable (rich) environment for the seed yield. In terms of secondary branches per plant and number of siliquae on branches, Pusa mustard 27 (EJ 17) showed adaptability across all types of environments. Therefore, Rohini (SC) and KMR-15-14 were identified as stable genotypes for optimum seed yield across different environmental conditions. Keyword: Mustard, G × E interaction, stability, , Regression, Deviation from regression, Brassica juncea

Study on gene action, combining ability and heterosis for different traits in Indian mustard (Brassica juncea L. Cxern & Coss)

Abstract Combining ability and heterosis was investigated in mustard hybrids obtained from 7 × 7 half diallel cross. Twenty one F1s along with seven parents were evaluated in Randomized Complete Block Design. The combined analysis of variance revealed very significant differences among the parents for all morphological variables with the exception of secondary branches and siliqua length, indicating wide diversity among the parental material used in the present study. Significant GCA and SCA variance indicated additive and non-additive gene action across all the characters. The SCA variance components were larger than the GCA variance components for most traits indicating the prevalence of non-additive gene effects. Jawahar mustard × PM-30, showed a significantly positive SCA and better parent heterosis for seed yield per plant whereas five hybrids (Kranti × PM-30, Gujarat Mustard-3 × Pusa Mahak, Jawahar Mustard × PM-30, Jawahar Mustard × Pusa Mahak and PM-30 × Pusa Mahak) exhibited significant positive SCA effect for number of siliqua/ plant. Jawahar Mustard × PM-30, Varuna×Jawahar Mustard, Gujarat Mustard-3 × PM-30, exhibited highly significant heterosis over the mid-parent. Keywords: Brassica juncea, GCA, SCA, gene action, Heterosis

Developing genomic tools to assist turnip rape [Brassica rapa (L.) subsp.oleifera (DC.) Metzg.] breeding

IntroductionTurnip rape is recognized as an oilseed crop contributing to environmentally sustainable agriculture via integration into crop rotation systems. Despite its various advantages, the crop’s cultivation has declined globally due to a relatively low productivity, giving way to other crops. The use of genomic tools could enhance the breeding process and accelerate genetic gains. Therefore, the present research investigated 170 turnip rape accessions representing its global gene pool to identify SNP markers associated nine phenological and agro-morphological traits and estimate the genomic breeding values (GEBVs) of the germplasm through GWAS and genomic prediction analyses, respectively.MethodsField trials were conducted at two sites in northern and southern Sweden to obtain the phenotypic data while genotyping was conducted via the genotyping-by-sequencing (GBS) method. The traits studied include days to flowering (DTF) and maturity (DTM), plant height (PH), seed yield (YLD), thousand seed weight (TSW), silique length (SL), number of siliques (NS), number of seeds per silique (SS), and pod shattering resistance (PSHR).Results and conclusionAnalysis of variance revealed substantial variation among accessions, with significant genotype-by-environment interaction for most traits. A total of 25, 17, 16, 14, 7, 5, 3, and 3 MTAs were identified for TSW, DTF, PH, PSHR, SL, YLD, SS and DTM, respectively. An 80%–20% training-test set genomic prediction analysis was conducted using the ridge regression – BLUP (RR-BLUP) model. The accuracy of genomic prediction for most traits was high, indicating that these tools may assist turnip rape breeders in accelerating genetic gains. The study highlights the potential of genomic tools to significantly advance breeding programs for turnip rape by identifying pivotal SNP markers and effectively estimating genomic breeding values. Future breeding perspectives should focus on leveraging these genomic insights to enhance agronomic traits and productivity, thereby reinstating turnip rape as a competitive and sustainable crop in Sweden and broader global agriculture.

Overexpression of forage millet (Setaria italica) SiER genes enhances drought resistance of Arabidopsis thaliana.

ERECTA (ER) is a type of receptor-like kinase that contributes a crucial mission in various aspects of plant development, physiological metabolism, and abiotic stresses responses. This study aimed to explore the functional characteristics of the SiER family genes in millet (Setaria italica L.), focusing on the growth phenotype and drought resistance of Arabidopsis overexpressed SiER4_X1 and SiER1_X4 genes (SiERs ). The results revealed that overexpression of SiER4_X1 and SiER1_X4 genes in Arabidopsis significantly enhanced the leaf number, expanded leaf length and width, further promoted the silique number, length and diameter, and plant height and main stem thickness, ultimately leading to a substantial increase in individual plant biomass. Compared to the wild-type (WT), through simulated drought stress, the expression level of SiER genes was notably upregulated, transgenic Arabidopsis seeds exhibited stronger germination rates and root development; after experiencing drought conditions, the activities of antioxidant enzymes (superoxide dismutase and peroxidase) increased, while the levels of malondialdehyde and relative electrical conductivity decreased. These results indicate that overexpression of SiERs significantly enhanced both biomass production and drought resistance in Arabidopsis . The SiER4_X1 and SiER1_X4 genes emerge as promising candidate genes for improving biomass production and drought resistance in forage plants.

Evaluation of the stability of quantitative traits of winter oilseed rape (Brassica napus L.) by AMMI analysis

Agronomical traits of crop plants exhibit quantitative variation that is controlled by multiple genes and is dependent on environmental conditions. The main objective of this study was to decipher the genotype-by-environment interaction (GEI) for six yield-related traits of 25 winter oilseed rape (WOSR) genotypes using the additive main effects and multiplicative interaction (AMMI) model. The genotypes chosen included canola cultivars, our newly developed WOSR breeding lines, yellow-seeded, semi-resynthesized and mutant genotypes, together with ogu-INRA F1 hybrids and their parental lines. These were tested in field trials at two locations over three growing seasons. Field experiments were conducted in a randomized block design with four replicates. We recorded the beginning of flowering, seed yield (SY) and SY components, the number of siliques per plant, the length of siliques, the number of seeds per silique, and the weight of 1000 seeds. The average SY in six environments varied from 16.55 to 41.64 dt·ha−1. The AMMI analysis showed significant effects of both G and E, as well as GEI, for the above traits. In this study, we observed that the climate condition, especially precipitation in addition to the soil type were the most influential factors on the SY and SY-trait value. Seed yield was positively correlated with: the number of siliques per plant, the length of siliques, the number of seeds per silique and the weight of 1000 seeds. We also found that our new ogu-INRA F1 hybrids, as well as cultivars Monolit, Mendel, Starter and Sherlock, showed stability for the analyzed traits.

Analysis of Genetic Architecture through Generation Mean Analysis for Yield and Yield Contributing Traits in Crosses of Indian Mustard (Brassica juncea)

Generation mean analysis was deployed to study the inheritance of 12 yield and its component traits in two crosses viz. PR-2009-6 × ALBELI (Family A) and PR-2009-6 × RGN-73 (Family B) of Indian mustard [Brassica juncea (L.) Czern& Coss] for parents P1 and P2, F1 , F2 , BC1 and BC2 generations. Analysis of variance showed presence of significant variability among families and also within families (among progenies) for various traits under study except for days to maturity, seeds per siliquae and siliquae length. Family A showed considerable interaction effects for siliquae on main raceme, number of seeds per siliquae and oil content. Family B displayed epistatic interactions for characters such as plant height, length of main raceme, siliquae on main raceme, siliquae length, oil content seed yield per plant and glucosinolate content. Fixable effects [d] and [i] were found important in the inheritance of plant height, seed yield per plant and glucosinolate content. Among non fixable effects, dominance [h] gene action was significantly important for determining length of main raceme, number of siliquae on main raceme, siliquae length, oil content, seed yield per plant and glucosinolate content. Interaction gene effects were found significant in controlling plant height, length of main raceme, number of secondary branches per plant, siliquae on main raceme, siliqua length,1000-seed weight, oil content, seed yield per plant and glucosinolate content. Thus, both main effects and epistatic effects were found important in controlling the various traits under study.

Exemplification of Manganese with Salicylic Acid on Growth, Antioxidants and Productivity of Indian Mustard (Brassica juncea L.)

Background: In India, mustard, an essential oilseed crop, encounters several challenges including diminished yield and environmental apprehensions. The diligent mix of micronutrients and plant growth regulators indicates the potential for enhancing mustard crop output while retaining environmental sustainability. Manganese and salicylic acid play a vital role in plant metabolism. Methods: The trial was planned with ten treatments with three replications under randomized complete block design. The treatments consisted of control, varying concentrations of salicylic acid (75, 150, 300 ppm), varying concentrations of manganese (0.25, 0.5, 0.75 mM MnSO4) and combinations of both. Result: The experimental findings indicate the synergistic use of salicylic acid and manganese resulted in enhanced growth dynamics and improved yield potential compared to the separate treatments of manganese and salicylic acid. The exogenous application of 0.5 mM manganese with 150 ppm salicylic acid increased plant height by 10.83%, total dry matter by 49.6%, chlorophyll content by 51.61%, number of siliquae by 81.94%, test weight by 5.8%, grain yield by 41.26% and stover yield by 48.76% over the control. Growth and yield attributes positively correlated with grain and stover yield. Hence the application of both 0.5 mM Mn with 150 ppm SA is feasible for enhancement of Indian mustard productivity.

Effect of Different Levels of Integrated Nutrient Management in Mustard (Brassica juncea L.)

A field experiment was conducted during the Rabi session of 2020-21 in Jaunpur district which lies Indo-Gangetic plains of Central Uttar Pradesh to study the effect of integrated nutrient management in mustard (Brassica juncea L.). The trial was laid down in randomized block design (RBD) with three replications and twelve treatments viz, T1 100% RDF, T2 100% RDF + Azotobacter, T3 100% RDF + Azotobacter + PSB, T4 75% RDF + 25% N from FYM, T5 75% RDF + 25% N from FYM + Azotobacter, T6 75% RDF + 25% N from FYM + Azotobacter + PSB, T7 50% RDF + 50% N from FYM, T8 50% RDF + 50% N from FYM + Azotobacter, T9 50% RDF + 50% N from FYM + Azotobacter + PSB, T10 25% RDF + 75% N from FYM, T11 25% RDF + 75% N from FYM + Azotobacter, T12 25% RDF + 75% N from FYM + Azotobacter + PSB. Result of this study revealed that there was significant effect on growth and yield parameters. Plant height, number of branches, number of siliques, length of silique, number of seed per siliqua, test weight, seed and stover yield in T6 which was statistically at par with T9, T8, T7, T3. Maximum yield (1807 kg/ha) was recorded in T6. It may be concluded that RDF with organic Manure (FYM) and Biofertilizer (Azotobacter + PSB) is possible to produce more yield in mustard crops cultivated without much soil productivity under Indo-Gangetic plains of central Uttar Pradesh.

Soil Test Crop Response Concerning Soil Properties and Yield Attributes of Mustard (Brassica juncea L.) var. Krishna

We experimented in the field at the central research farm of the Department of Soil Science and Agricultural Chemistry, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj during Rabi season of 2022-23. The soil in the experimental area was characterized by a sandy loam texture. There were 27unit plots in total, with each plot sized at 2 X 2 m. We used a randomized block design, incorporating different levels of NPK (80:40:40), vermicompost, and two levels of sulfur (50% and 100%). The T9 (STCR+ 5t ha-1 Vermicompost+ 100% S) demonstrated that although there was a slight decrease in pH, bulk density, and particle density, there was a significant (P<0.05) increase in pore space, water holding capacity, electrical conductivity (EC), organic carbon, and the availability of nitrogen, phosphorus, and potassium. Improvements were observed in plant growth and yield attributes, which showed the best results in terms of plant height, number of siliquae per plant, and total mustard yield compared to the control conditions. However, the use of organic manures and their combination with a full NPK treatment significantly enhanced the growth and overall yield attributes of mustard.

The Results of a Pre-study to Determine The Effects of Zinc and Iron Foliar Fertilizer on Canola (Brassica napus L.)

Increasing canola yield, a winter oil crop, is important for its cultivation in the product pattern of the Mediterranean climate zone. Therefore, a study was planned to determine the impact of foliar application zinc (Zn), iron (Fe) and Zn + Fe combination on the yield and quality of canola. The experiment was arranged in a Randomized Complete Block Design with four replications. Zinc (3.0% v/v), iron (7.5% w/w), and their combination were foliar sprayed at rosette and flowering stages. The highest number of siliques per plant, number of seeds per silique, seed index, and seed yield were recorded in the Fe application. Zn + Fe combination significantly increased the oil and protein content, whereas Zn application exhibited higher oil and protein yield performance. It was concluded that using iron and zinc separately regarding yield and quality is economically beneficial.

Correlation Studies of Morpho-physiological Characters with Seed Yield in Rapeseed (Brassica rapa var. Toria)

Background: In the North-Eastern states, Assam has the highest area of cultivation of rapeseed and found to be potential hub for increasing productivity to a great extent. To fulfil the increasing demand for edible oils, interdisciplinary approaches including physiological parameters must be paid off. The suitable genotype for a particular region has to be identified based on physiological efficiency and yield. Methods: A field experiment was carried out at the Instructional-cum-Research (ICR) Farm, Assam Agricultural University, Jorhat-13, Assam during rabi seasons of 2021-22 and 2022-23. The experiment was laid out in randomized block design with three replications comprising of 22 different genotypes of rapeseed including TS-38 (Check), TS-46, TS-67, TS-36, TS-29, TS-75-1, TS-75-1TL, TS-75-2ME, TS-75-2-MM, TS-76-1, TS-76-2, JT-90-1, Panchali, Bhawani, CG Toria-4, TKM-20-1, TKM-20-2, JT-14-5, PT-2018-09, CG Toria-3, Tapeshwari and PT-303. The crops were grown following the recommended package of practices. All the morpho-physiological parameters, yield attributes and yield were recorded following the standard methodologies. The correlation coefficients of morpho-physiological characters and different yield components with seed yield were worked out from the pooled values of two years following the standard method. Result: The results indicated that number of green leaves per plant, stem diameter, total dry weight, total leaf chlorophyll content, AGR and CGR showed highly significant positive correlation with seed yield, clearly indicating their contribution towards higher yield in rapeseed. The number of primary branches and SLW exhibited significant positive correlation with seed yield. Among the yield attributes, number of siliqua per plant, seeds per silique, seeds per plant, sink capacity, stover yield and harvest index showed a highly significant positive correlation with the seed yield.

Contrasting Impacts of Ubiquitin Overexpression on Arabidopsis Growth and Development.

In plants, the ubiquitin (Ub)-26S proteasome system (UPS) regulates numerous biological functions by selectively targeting proteins for ubiquitylation and degradation. However, the regulation of Ub itself on plant growth and development remains unclear. To demonstrate a possible impact of Ub supply, as seen in animals and flies, we carefully analyzed the growth and developmental phenotypes of two different poly-Ub (UBQ) gene overexpression plants of Arabidopsis thaliana. One is transformed with hexa-6His-UBQ (designated 6HU), driven by the cauliflower mosaic virus 35S promoter, while the other expresses hexa-6His-TEV-UBQ (designated 6HTU), driven by the endogenous promoter of UBQ10. We discovered that 6HU and 6HTU had contrasting seed yields. Compared to wildtype (WT), the former exhibited a reduced seed yield, while the latter showed an increased seed production that was attributed to enhanced growth vigor and an elevated silique number per plant. However, reduced seed sizes were common in both 6HU and 6HTU. Differences in the activity and size of the 26S proteasome assemblies in the two transgenic plants were also notable in comparison with WT, suggestive of a contributory role of UBQ expression in proteasome assembly and function. Collectively, our findings demonstrated that exogenous expression of recombinant Ub may optimize plant growth and development by influencing the UPS activities via structural variance, expression patterns, and abundance of free Ub supply.

Interactive Effect of Nitrogen, Zinc (Zn) and Iron (Fe) on the Growth and yield Characteristics of Gobhi Sarson (Brassica napus L.)

Brassica napus L., commonly known as Gobhi Sarson in India, is a vital oilseed crop contributing significantly to the agricultural economy. India stands as the fourth-largest producer of oilseeds, with mustard-rapeseed, including Brassica napus, constituting about 28.6% of the total oilseed production [1]. The experiment titled "Study on Foliar Application of Nitrogen, Zinc (Zn), and Iron (Fe) on the Growth and Yield Characteristics of Gobhi Sarson (Brassica napus L.)" was conducted at the research farm of Lovely Professional University, Punjab, during the Rabi season of 2023-24. A total of nine treatments with three replications were evaluated using a Randomized Block Design. The findings indicate that Treatment 9 (75%RDF + 1% Urea + 0.5% FeSO4 + 0.5% ZnSO4) demonstrated the highest plant height, fresh weight, dry weight, leaf area, primary and secondary branches per plant, number of siliquae per plant, seeds per siliquae, 1000-seed weight, seed yield, biological yield, and oil content. Treatment 5 (50%RDF + 1% Urea + 0.5% FeSO4 + 0.5% ZnSO4) also performed well across these parameters, albeit to a slightly lesser extent. (T9) recorded the highest seed yield (19.7 q ha-1), significantly outperforming all other treatments, with Treatment 5 being statistically comparable. Moreover, Treatment 9 exhibited the maximum oil content (41.2%), followed closely by Treatment 5 with oil content of 40.6%. Conversely, the control treatment (T1) yielded the lowest oil content. These results underscore the efficacy of foliar application of 75% RDF with 1% Urea, 0.5% FeSO4, and 0.5% ZnSO4 in enhancing the growth and yield characteristics of Gobhi Sarson, with Treatment 5 showing similar trends.

Detecting directional epistasis and dominance from cross-line analyses in alpine populations of Arabidopsis thaliana.

The contribution of non-additive genetic effects to the genetic architecture of fitness and to the evolutionary potential of populations has been a topic of theoretical and empirical interest for a long time. Yet, the empirical study of these effects in natural populations remains scarce, perhaps because measuring dominance and epistasis relies heavily on experimental line crosses. In this study, we explored the contribution of dominance and epistasis in natural alpine populations of Arabidopsis thaliana for 2 fitness traits, the dry biomass and the estimated number of siliques, measured in a greenhouse. We found that, on average, crosses between inbred lines of A. thaliana led to mid-parent heterosis for dry biomass but outbreeding depression for an estimated number of siliques. While heterosis for dry biomass was due to dominance, we found that outbreeding depression for an estimated number of siliques could be attributed to the breakdown of beneficial epistatic interactions. We simulated and discussed the implication of these results for the adaptive potential of the studied populations, as well as the use of line-cross analyses to detect non-additive genetic effects.

An auxin regulated Universal stress protein (TaUSP_3B-1) interacts with TaGolS and provides tolerance under drought stress and ER stress.

A previously identified wheat drought stress responsive Universal stress protein, TaUSP_3B-1 has been found to work in an auxin dependent manner in the plant root tissues in the differentiation zone. We also found a novel interacting partner, TaGolS, which physically interacts with TaUSP_3B-1 and colocalizes in the endoplasmic reticulum. TaGolS is a key enzyme in the RFO (Raffinose oligosaccharides) biosynthesis which is well reported to provide tolerance under water deficit conditions. TaUSP_3B-1 overexpression lines showed an early flowering phenotype under drought stress which might be attributed to the increased levels of AtTPPB and AtTPS transcripts under drought stress. Moreover, at the cellular levels ER stress induced TaUSP_3B-1 transcription and provides tolerance in both adaptive and acute ER stress via less ROS accumulation in the overexpression lines. TaUSP_3B-1 overexpression plants had increased silique numbers and a denser root architecture as compared to the WT plants under drought stress.

Overexpression of Larch SCL6 Inhibits Transitions from Vegetative Meristem to Inflorescence and Flower Meristem in Arabidopsis thaliana (L.) Heynh.

SCARECROW-LIKE6 (SCL6) plays a role in the formation and maintenance of the meristem. In Larix kaempferi (Lamb.) Carr., an important afforestation tree species in China, SCL6 (LaSCL6) has two alternative splicing variants-LaSCL6-var1 and LaSCL6-var2-which are regulated by microRNA171. However, their roles are still unclear. In this study, LaSCL6-var1 and LaSCL6-var2 were transformed into the Arabidopsis thaliana (L.) Heynh. genome, and the phenotypic characteristics of transgenic A. thaliana, including the germination percentage, root length, bolting time, flower and silique formation times, inflorescence axis length, and branch and silique numbers, were analyzed to reveal their functions. It was found that LaSCL6-var1 and LaSCL6-var2 overexpression shortened the root length by 41% and 31%, respectively, and increased the inflorescence axis length. Compared with the wild type, the bolting time in transgenic plants was delayed by approximately 2-3 days, the first flower and silique formation times were delayed by approximately 3-4 days, and the last flower and silique formation times were delayed by about 5 days. Overall, the life cycle in transgenic plants was prolonged by approximately 5 days. These results show that LaSCL6 overexpression inhibited the transitions from the vegetative meristem to inflorescence meristem and from the flower meristem to meristem arrest in A. thaliana, revealing the roles of LaSCL6-var1 and LaSCL6-var2 in the fate transition and maintenance of the meristem.