Abstract This study aims to investigate the factors that influence the success of artificial insemination, the effect of successful artificial insemination on livestock rearing, and the income of beef cattle farmers in Palon Village, Jepon District, Blora Regency. The research method is quantitative descriptive with a survey approach. Primary data were obtained through direct observations, interviews, and questionnaires with beef cattle farmers in Palon Village and inseminators of the Food, Agriculture, Livestock, and Fisheries Office (DP4) of Blora Regency, while secondary data were obtained from DP4 and the Statistics Indonesia (BPS) of Blora Regency. Multiple linear regression and beef cattle farming analysis were used in this study. The findings show that the number of repeat breeding cases in Palon Village remains significantly high. Several factors were identified as influencing the success of artificial insemination, including female livestock characteristics, thawing and handling semen, breeder conditions, and inseminators’ skills and attitudes. Livestock health, administration of anthelmintics and vitamins, rectal palpation examination, body condition score, age of the breeder, feed, and the origin or breed of the livestock are all important characteristics of female livestock. The handling and thawing of semen were discovered to be critical factors influencing the type of straw used. Farmer conditions, which include age, education level, length of time involved in beef cattle farming, basic livelihood, and the number of owners, also play a role. Age, experience in the field, and the extent of training received were discovered to influence the success of artificial insemination. Repeated mating contributes to increased maintenance costs and the costs associated with artificial insemination in beef cattle.