Seventy-seven (77) potato accessions were collected from production areas in Western highlands of Cameroon (5°10’-6°30’N and 9°30’- 10° 80’E). Out of the 77 accessions, 69 were traditional and 8 were modern varieties. The genotypes were grown under Menoua (5°27’N, 10°04’E) ecological conditions. 23 morphological and agronomical traits from the internationally accepted descriptor list for potato were used. When considering 7 agronomic traits used in this study, there were no significant difference among collection zone and among altitudes; however, highly significant differences among the accessions were found. Dried matter content (21.25%), total tubers number (15.55), mean eyes number (10.46) and mean stem number (3.21) were significantly higher in traditional varieties than in modern varieties which however had the highest percentage of marketable tubers (84.89%). Significant correlations were found between marketable tuber number and plant height (r = 0.44; p ≤ 0.0001) and between dried matter content, mean stems number (r = 0.38; p ≤ 0.001), mean eyes number (r = 0.30; p ≤ 0.01) and total tubers number (r = 0.44; p ≤ 0.0001). Cluster analysis identified 2 distinct groups with high level of variation. Significant differences were observed on all agronomic traits between the 2 groups; the first group was made up of Forty-seven (47) individuals, all of them were traditional varieties out of the sixty-nine (69) collected; The second group comprised et mixture of local and exotic varieties. Implications of these results in connection with the potato breeding programs in Cameroon are discussed.