Number Of Malignant Tumors Research Articles

Dynamics of the abdominal organs malignant tumors regional morbidity, taking into account certain factors

The aim of the work is to determine the dynamics of the incidence of malignant neoplasms of the abdominal cavity in the Bukovina region and possible determinants. Material and methods. The study was performed on the basis of RMNE "Chernivtsi Regional Clinical Oncology Center" and Clinic of Oncology of Bukovinian State Medical University and covers observation materials during 2015-2019 and statistical reporting on 102,247 cases of tumors of various localizations. The indicators were calculated using Access and Excel software packages based on the Microsoft Office environment. The results. The total number of malignant neoplasms of different localizations is relatively stable throughout the study period and ranged from 19903 to 21259 cases per year (average 20449.4±508.2 registered during the year). Neoplasms of the abdominal cavity are about the third of the total number of tumors of different localization with a certain tendency to grow. The distribution of patients with neoplasms of the abdominal cavity by stages of development is relatively uniform, despite significant variations in different localizations of tumors. Conclusions. A significant part of tumors of the abdominal cavity is diagnosed in the late (III-IV) stages of the pathological process, which leads to a decrease in the effectiveness of treatment. Significant differences in the clinical and epidemiological characteristics of neoplasms of various abdominal organs have been identified, which requires further study.

RELATIONSHIP BETWEEN THE BLOOD LEVELS OF INFLAMMATORY CYTOKINES AND THE NUMBER OF CIRCULATING TUMOR CELLS WITH RESPONSE TO STANDARD CHEMOTHERAPY IN PATIENTS WITH OVARIAN CANCER

Introduction. Serum chemokines are inflammatory mediators, which role is shown in the occurrence and progression of a number of malignant tumors. Produced by white blood cells, stem cells, tumor and endothelial cells, chemokines control their movement and positioning. Chronic inflammation underlies the progression of ovarian cancer (OC ). This increases the likelihood of chemokines stimulating or blocking tumor progression.The aim of the study was to evaluate the relationship between the blood levels of inflammatory cytokines in blood and the number of circulating tumor cells (CTC s) with the response to standard chemotherapy (CT ) in patients with cancer.Material and Methods. In patients with primary OC before and after 2–4 courses of chemotherapy and in patients with benign ovarian tumors (as a control), serum levels of CCL 2, CCL 3, CCL 4, CXCL 8 and CX3CL 1 were evaluated by multiplex xMAP analysis. The amount of CTC s (population CD 45-/ Epcam+/CK+) was determined using a flow cytometer. Patients with ovarian cancer were divided into 3 groups according to the platinum sensitivity criterion of GC JG 4th, and progression-free interval (PFI) was determined. Results. It was found that the levels of CCL 2, CCL 3, CCL 4, CXCL 8, and CX3CL 1 in case of OC did not significantly differ from that in the control, strongly negatively correlated with age (except for the CCL 2 level). CT significantly increased the level of CCL 2 in the group of refractory OC ; of CCL 3 – in the group of sensitive OC , of CCL 4 – in the groups of resistant and sensitive OC , and C XCL 8 level increased in the groups with resistant and sensitive OC and decreased in the group of refractory OC . The number of CTC s in patients with OC was significantly higher than in the control. After CT , a decrease in the amount of CTC s strongly and significantly correlated with a decrease in the level of CX3CL 1 in the groups of refractory andsensitive OC . The maximum PFI occurred with an increase in serum levels of CCL 3, CXCL 8, a decrease in CCL 4 and a constant level of CX3CL 1.Conclusion. Thus, no significant differences in the levels of CCL 2, CCL 3, CCL 4, and IL -8 between patients with OC and control groups were found. The levels of chemokines studied and the amount of CTC s differed in the groups divided by the tumor sensitivity to CT . We observed significant correlations between the amount of CTC s and the level of CX3CL 1 in the group of platinumsensitive OC .

Efficacy of liver metastasectomy in pancreatic cancer

Background: Standard treatment for metastatic pancreatic cancer is systemnic chemotherapy. Significance of liver metastasectomy in pancreatic cancer remains unknown. This study aims to investigate efficacy of liver metastasectomy in pancreatic cancer. Methods: Between 2001 and 2019, 14 cases who underwent liver metastasectomy for pancreatic cancer at Tohoku University Hospital, including 7 synchronous metastasis cases and 7 metachronous metastasis cases, were retrospectively investigated. Results: Synchronous metastasis: Simultaneous resection of primary pancreatic tumor and liver metastases was performed in 5 cases, including 4 cases with neoadjuvant therapy and one cases with upfront surgery. Two-stage resection, which means prior metastasectomy and subsequent resection of primary tumor after chemotherapy, was performed in 2 cases. The number of metastatic tumors was within 3 in 6 cases. Median survival time (MST) after surgery, which was calculated from the primary tumor resection date in two-stage resection, was 12.5 months. One case with two-stage resection has been alive without recurrence for 81 months after surgery. Metachronous metastasis: The median time between primary tumor resection and liver metastases was 10.7 months. All cases were treated with systemic chemotherapy before liver metastasectomy. Duration of chemotherapy before metastasectomy was 3 months in 1 case, 6-12 months in 4 cases, and 12-24 months in 2 cases. The number of metastatic tumors was within 2 in all cases. MST after metastasectomy was 19.8 months. Conclusion: There are cases who has benefit of metastasectomy in liver metastasis of pancreatic cancer.

Neoplasias malignas em cavidade oral associadas ao uso de narguilé: Revisão integrativa da literatura

Tobacco is the main risk factor for oral cancer, with hookah/waterpipe being a device used to smoke tobacco, originally from India. According to the World Health Organization, an hour of hookah session is equivalent to smoking 100 units of ordinary cigarettes. It was believed that the hookah caused less damage to health than the conventional cigarette, but there are already scientific data that disagree with this belief. It was believed that the toxins present in the smoke would be 'filtered' when passing through the water of the device, however, the water has the function of cooling the smoke, facilitating deeper inhalation, together with the toxins. In this perspective, evaluating the increase in tobacco consumption through the hookah, this integrative review aims to research on malignant neoplasms in the oral cavity caused using the hookah, and its relationship with the increase in the number of malignant neoplasms in the oral cavity.

Dextran Sulfate Effects EMT of Human Gastric Cancer Cells by Reducing HIF-1α/ TGF-β.

The peritoneal implant metastasis is one of the main pathway and main cause for high mortality for gastric cancer metastasis. Researchs show that epithelial-mesenchymal transition (EMT) playing essential role in modulating gastric cancer metastasis, and the expression of hypoxia inducible factor-1α (HIF-1α) can promote EMT in tumor cells. This research aims to explore the influence and mechanism of Dextran Sulfate (DS) affecting EMT of human gastric cancer. In the present study, we found that DS can enter into the cytoplasm and function in it. Inhibition of HIF-1α or DS significantly inhibit the migration and invasion of human gastric cancer cells, and decrease the mRNA and protein expressions of HIF-1α, matrix metalloproteinase-2 (MMP-2), transforming growth factor-β (TGF-β), Twist and N-cadherin (N-cad), rise E-cadherin (E-cad) expression, DS with HIF-1α knockdown has a stronger effect. In vivo studies indicated that compared with using DS or HIF-1α knockdown alone, DS with HIF-1α knockdown can better suppress the volume and number of metastatic tumors, and reduce the mRNA and protein expressions of HIF-1α, MMP-2, TGF-β, Twist and N-cad in metastatic tumor tissues of nude mice. We further demonstrated that the expression of HIF-1α, MMP-2, TGF-β , Twist and N-cad were higher in well and poorly differentiated gastric cancer than paracancerous tissue, and poorly differentiated gastric cancer were even higher, while E-cad expression was opposite. Taken together, this study shows that DS can interfere the expression of HIF-1α, thereby inhibiting TGF-β-mediated EMT of gastric cancer cells, and demonstrated a promising application of DS in gastric cancer therapy.

Percutaneous ethanol injection enhanced the efficacy of radiofrequency ablation in the treatment of HCC: an insight into the mechanism of ethanol action

Purpose To discuss the possible reasons why percutaneous intratumoral ethanol injection (PEI) combined with radiofrequency ablation (RFA) to treat hepatocellular carcinoma (HCC) reduced the recurrence and metastasis compared with RFA alone. Materials and methods Forty VX2 tumor-bearing rabbits were randomly divided into four groups (n = 10): the PEI, RFA, PEI-RFA, and control groups. Five rabbits from each group were sacrificed on the 3rd and 7th days after ablation. The number of metastatic tumors in the lung was counted. The ablation volume was measured, and residual tumor specimens were prepared for hematoxylin and eosin staining and caspase-3, Ki-67, and VEGF immunohistochemical staining. Results The volume of ablation in the PEI-RFA group was significantly larger than that in the RFA and PEI groups (p < 0.05). However, no significant differences in the number of lung metastases after ablation were observed among the groups (p > 0.05). The number of microthrombi in the PEI-RFA group was greater than that in the control and RFA groups (p < 0.001 and p < 0.05). The Ki-67 labeling index (LI) and H-score of VEGF in the PEI-RFA group were lower than those in the RFA group, while the H-score of caspase-3 was higher than that in the RFA group on the 7th day after ablation (p < 0.05). Conclusion PEI occluded blood vessels by inducing microthrombi formation, and thereby reducing heat dissipation and increasing the effect of RFA. More importantly, in comparison with an incomplete RFA, PEI-RFA inhibited the increase in the Ki-67 and VEGF expression levels and the decrease in the caspase-3 expression level to happen at some extent and therefore improved the prognosis.

The role of defining the sentinel lymph node in the treatment of low-risk highly differentiated thyroid cancer (review of literature)

The definition of a sentinel lymph node is a technique that appeared at the middle of the 20th century. For a number of malignant neoplasms, the definition of a sentinel lymph node is standard procedure, but for highly differentiated thyroid cancer, the data is conflicting. The role of prophylactic central compartment lymph node dissection in the clinical N0 stage is not established. Sentinel lymph node detection seems to be the possible way to reduce the risk of persistence and recurrence of highly differentiated thyroid cancer.

Risk factors for short recurrence-free survival after resection of pancreatic neuroendocrine tumor (PanNET) liver metastases: which patients should undergo resection?

Background: In the treatment of metastatic pancreatic neuroendocrine tumors (PanNETs), surgical resection is the first choice if curative resection is expected. However, most patients develop recurrence after resection of liver metastasis. Because one of the benefits of resection is to gain a tumor-free period for the patients, it is important to identify which patients achieve longer recurrence-free survival (RFS) by resection. In this study, the clinicopathological factors associated with RFS after resection of metastatic PanNETs in the liver were evaluated to identify the patient group that is suitable for resection.Methods: Consecutively diagnosed patients with PanNET liver metastasis with resection at our hospital from January 2000 to July 2019 were evaluated. A total of 26 metastatic PanNET patients with primary liver resections were evaluated. The median follow-up time was 48.3 months.Results: There were 18 NET recurrences of the total 26 resections, with a median RFS of 17.9 months. Independent risk factors for short RFS were a high Ki67 index (p = .009) and the number of resected tumors (p = .045). When the cut-off value for the Ki67 index was 5.0% and that for the number of resected tumors was 6, Ki67 > 5.0% tumors had shorter RFS (4.9 months vs. 38.2 months p = .006), and patients with tumors > = 7 tumors had shorter RFS (4.7 months vs. 27.5 months p = .001).Conclusions: These findings indicate that good candidates for resection of metastatic tumors of PanNETs could be patients with low Ki67 tumors and a small number of metastatic tumors.

Genomic characterization of human brain metastases identifies drivers of metastatic lung adenocarcinoma.

Brain metastases from lung adenocarcinoma (BM-LUAD) cause significant patient mortality. To identify genomic alterations that promote brain metastases, we performed whole-exome sequencing of 73 BM-LUAD cases. Using case-control analyses, we discovered candidate drivers of brain metastasis by identifying genes with more frequent copy-number aberrations in BM-LUAD compared to 503 primary lung adenocarcinomas. We identified three regions with significantly higher amplification frequencies in BM-LUAD, including MYC (12% vs 6%), YAP1 (7% vs 0.8%), and MMP13 (10% vs 0.6%) and significantly more frequent deletions in CDKN2A/B (27% vs 13%). We confirmed that amplification frequencies of MYC and YAP1/MMP13 were elevated in an independent cohort of 105 patients. Functional assessment in patient-derived xenograft mouse models validated that MYC, YAP1 or MMP13 overexpression increased the brain metastasis incidence. These results demonstrate that somatic alterations contribute to brain metastases and that genomic sequencing of a large number of metastatic tumors can reveal novel metastatic drivers.

The study of rate and spectrum of frequency of mutations of gene KRAS in patients with colorectal cancer in the Republic of Kazakhstan

The sampling included tumor material of 332 patients diagnosed with colorectal cancer, fixed in formalin and enclosed in paraffin, and treated in oncology dispensaries and centers and in the Kazakh Research Institute of Oncology and Radiology in 2010-2014. After morphological evaluation of the material quality, the molecular genetic analysis was applied to establish mutation of the KRAS gene using polymerase chain reaction in real-time. The study established that KRAS mutation rate in patients with colorectal cancer had no reliable dependency with the region of their residence, since statistical analysis of pair "region of residence - all KRAS mutations" had value of correlation coefficient rp = 0.1 (p = 0.05). colorectal cancer It was established that there is no reliable dependence of rate of KRAS gene mutation in patients with colorectal cancer in the region of their residence. The high rate of occurrence of mutated type of gene was detected in 3 regions (Kyzyl-Orda, North Kazakhstan and Almaty) within the range of 58.3-76.5% of cases. The wild type of KRAS gene was most frequently detected in patients from the Karaganda region (65.5%), South Kazakhstan (71.4%) and Almaty (60.7%).

Thermal Ablation Induces Transitory Metastatic Growth by Means of the STAT3/c-Met Molecular Pathway in an Intrahepatic Colorectal Cancer Mouse Model.

Background Systemic protumorigenic effects have been noted after radiofrequency ablation (RFA) of normal liver and have been linked to an interleukin 6/signal transducer and activator of transcription 3 (STAT3)/hepatocyte growth factor (HGF)/tyrosine-protein kinase Met (c-Met)/vascular endothelial growth factor (VEGF) cytokinetic pathway. Purpose To elucidate kinetics of RFA protumorigenic effects on intrahepatic metastatic implantation and growth and determine potential molecular targets for pharmacologic suppression of these effects. Materials and Methods An intrahepatic metastasis model was established by implanting CT26 and MC38 tumor cells into 216 7-8-week-old male Balb/C and C57BL6 mice, respectively, by means of splenic injection. Between June 2017 and March 2019, mice underwent tumor injection, followed 24 hours later by either standardized RFA (70°C ± 1, 5 minutes, 1-cm tip) or a sham procedure (needle placement without heating) (12 animals per arm, n = 48). Next, RFA or sham procedures were performed, followed by splenic tumor cell injection at 1 day, 3 days, or 7 days later (six animals per arm, n = 72). Finally, PHA-665752 and S3I-201 were used to block c-Met or STAT3, respectively, prior to either RFA or sham treatment (six animals per arm, n = 96). Livers were harvested at 14 days for CT26 and 21days for MC38 for tumor quantification. Ki-67 and CD34 immunohistochemistry measured proliferative indexes and microvascular density, respectively. Data were compared with analysis of variance and the two-tailed Student t test. Results RFA performed after tumor cell injection induced increased metastatic tumor number (103 ± 45 vs 52 ± 44 [CT26], P = .009 and 87 ± 51 vs 39 ± 20 [MC38], P = .007), cellular proliferation (P < .001 for both), and intratumoral neovascularization (P < .001 for both), compared with the sham procedure. Tumor cell injection performed 1 day and 3 days after RFA also increased these indexes (P < .05), while no difference was demonstrated for cell injection 7 days after RFA (P > .05). Adjuvant c-Met or STAT3 inhibition reduced intrahepatic metastatic parameters after RFA to baseline (P < .03), equivalent to the sham group (P > .05). Conclusion Radiofrequency ablation of normal liver promotes intrahepatic metastatic implantation and increased growth over a short-lived (1-3 days) temporal window in animal models. This phenomenon can be potentially neutralized with specific inhibition of pathways including hepatocyte growth factor/tyrosine-protein kinase Met and signal transducer and activator of transcription 3. © RSNA, 2019 Online supplemental material is available for this article. See also the editorial by Nikolic in this issue.

В чем причины изменения профиля онкологической заболеваемости населения Северо-Восточной Сибири за последние 100 лет (на примере Якутии)?

The structure of the oncological incidence among the population of Yakutia has changed significantly over the past 100 years. During this period, esophageal and gastric cancers lost the leading positions in the structure of oncological pathology; there was a sharp upsurge in the number of lung cancer and its decline by the end of the analyzed period; there has been a rise in the number of malignant neoplasms of the reproductive organs and large intestine. It should be noted that a sharp decrease in the number of esophageal cancer among the region’s population was due not to only the improvement of socio-economic conditions, but also to the well-coordinated and focused work of practical healthcare coordinated by the research team of Professor Ara Bezrodnykh that focused on the early diagnosis and prevention of malignant neoplasms of the upper digestive tract. The early 21st century was characterized by the beginning of the formation of the structure of oncological morbidity connected with the post-industrial development in Yakutia. The reasons for the recent changes are still being analyzed to be clarified.

Prognostic and metastasis-related factors in colorectal neuroendocrine tumors: A cross-sectional study based on the Surveillance, Epidemiology and End Results.

The incidence of colorectal neuroendocrine tumors (NETs) is gradually increasing with the increasing availability of colonoscopy and computed tomography. However, prognostic and metastatic factors for colorectal NETs are unknown. The aim of the present study was to identify clinicopathological prognostic and metastasis-related risk factors for colorectal NETs. Based on the Surveillance, Epidemiology and End Results (SEER) program, the data of 3,093 patients with colorectal NETs were included in the present study. χ2 test, crosstabulation, Kaplan-Meier method, Cox proportional hazard regression model, nomogram and logistic regression analysis were used to evaluate the risk factors. Systemic analysis of data from 3,093 patients revealed that age (P<0.001), marital status (P<0.001), number of malignant tumors (P<0.001), histopathological grade (P<0.001) and clinical stage (P<0.001) were independent prognostic factors of cancer-specific survival (CSS). Among them, married patients exhibited longer CSS compared with unmarried patients (P<0.05). A nomogram based on these five factors with an area under the curve of 0.921 [95% confidence interval (CI), 0.908–0.934; P<0.001] was constructed. Age (P=0.010), primary tumor site (P<0.001), surgery (P<0.001), tumor size (P<0.001) and histopathological grade (P<0.001) were risk factors for distant metastasis of colorectal NETs. In conclusion, age, marital status, number of malignant tumors, histopathological grade and clinical stage may be independent prognostic factors for CSS of colorectal NETs, and the nomogram may have higher predictive efficiency compared with clinical stage. Age, primary tumor site, surgery, tumor size and histopathological grade may be risk factors for the distant metastasis of colorectal NETs.

Role of non-coding RNA in pancreatic cancer.

Pancreatic cancer is a malignant disease that develops rapidly and carries a poor prognosis. Currently, surgery is the only radical treatment. Non-coding RNAs (ncRNAs) are protein-free RNAs produced by genome transcription; they play important roles in regulating gene expression, participating in epigenetic modification, cell proliferation, differentiation and reproduction. ncRNAs also play key roles in the development of cancer; microRNA (miRNA) and long non-coding RNA (lncRNA) may lead the way to new treatments for pancreatic cancer. miRNAs are short-chain ncRNAs (19–24 nt) that inhibit the degradation of protein translation or their target gene mRNAs to regulate gene expression. lncRNAs contain >200 nt of ncRNA and play important regulatory roles in a number of malignant tumors, in terms of tumor cell proliferation, apoptosis, invasion and distant metastasis. lncRNAs can be exploited for the diagnosis and treatment of pancreatic cancer and have substantial prospects for clinical application. Nevertheless, the molecular mechanism of their regulation and function, as well as the significance of other ncRNAs, such as piwi-interacting RNA, in the pathogenesis of pancreatic cancer, are largely unknown. In this review, the structures of ncRNAs with various classifications, as well as the functions and important roles of ncRNAs in the diagnosis and treatment of pancreatic cancer are reviewed.

Silencing of estrogen receptor β promotes the invasion and migration of osteosarcoma cells through activating Wnt signaling pathway.

PurposeThis study aimed to evaluate the specific roles of estrogen receptor β (ERβ) on the invasion and migration of osteosarcoma (OS) cells and explore the regulatory mechanisms relating with Wnt signaling pathway.MethodsThe expression of ERβ was detected in human OS tissues by quantitative real-time PCR and immunohistochemistry. U2-OS cells were transfected with siRNA-ERβ (si-ERβ) to downregulate ERβ and treated with FH535 to inhibit Wnt signaling. The migration and invasion ability was detected by scratch and transwell assay, respectively. The expression of β-catenin, MMP-7, and MMP-9 was detected by Western blot. Subcutaneous tumor-bearing model was established by injection of U2-OS cells into mice, and the tumor volumes were measured. Orthotopic transplantation model was established by transplantation of tumor tissues into the liver of mice, and the metastatic tumors were counted.ResultsERβ was downregulated in human OS tissues and U2-OS cells. The transfection of si-ERβ significantly increased the scratch healing rate; the number of invasion cells; and the expression of β-catenin, MMP-7, and MMP-9 in U2-OS cells. The injection of si-ERβ-transfected U2-OS cells into mice significantly increased the subcutaneous tumor volume; the expression of β-catenin, MMP-7, and MMP-9; and the number of metastatic tumors in liver tissues. The promoting effects of si-ERβ on the invasion and migration of U2-OS cells were significantly reversed by FH535 in vitro and in vivo.ConclusionSilencing of ERβ promotes the invasion and migration of OS cells via activating Wnt signaling pathway.

Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model

Immune checkpoint inhibitors (ICIs) have become an effective therapeutic option for colorectal cancer and studies on these drugs have therefore increased greatly. Efficacy assessments of ICIs in preclinical orthotopic colorectal cancer using MRI have not been reported however due to the difficulties in conducting colorectal imaging. The purpose of this present study was to investigate the feasibility of using magnetic resonance colonography (MRC) to evaluate the efficacy of an ICI, an anti-PD-L1 antibody, in an orthotopic colorectal cancer mouse model. The mouse model was generated by the engraftment of colorectal cancer cells into the submucosal layer of the colon. Anti-cancer efficacy was assessed by tumor volume and metastatic tumor number analyses, and these values were significantly lower in the PD-L1 antibody-treated group compared to the controls. Histological analyses using H&E and Ki-67 immunohistochemical staining confirmed a highly efficacious tumor growth inhibition and enhanced infiltration by CD4+ and CD8+ lymphocytes in the PD-L1 antibody-treated group. We conclude that MRC has the potential to be used for ICI efficacy assessments against orthotopic colorectal cancer mouse model.

A survey of tumors affecting cattle, buffaloes and sheep, in El-Dakahlyia Governorate

In a 2-year survey of tumors occurring in ruminants reared in El-Dakahlyia Governorate, Egypt during the period from October 2016 to October 2018. 66 tumor masses were collected: 60 from cattle, 2 from buffaloes and 4 from sheep. According to histopathology of cattle tumors, 15 neoplasms were diagnosed as cutaneous papilloma, 5 neoplasms were diagnosed as cutaneous fibropapillomas, 25 neoplasms were diagnosed as squamous-cell carcinoma (SCC) of the eye; 8 neoplasms were diagnosed as SCC of perineum, 1 neoplasm was diagnosed as SCC in skin of muzzle, 2 neoplasms were diagnosed as epulis in dental pad, 2 neoplasms were diagnosed as vaginal leiomyoma, 1 neoplasm was diagnosed as fibroma and 1 neoplasm was diagnosed as liposarcoma. Histopathology of buffalo tumors revealed SCC in head skin (1 case) and buccal cavity (1 case). Histopathology of sheep tumors revealed SCC in skin under the tail (2 cases), skin of groin (1 case) and skin of abdomen (1 case). In conclusion, cattle (60/66) were more susceptible to tumors than buffaloes (2/66) and sheep (4/66). Females accounted for a higher percentage of the neoplasms (47/66) than males (19/66). Adult animals aged above 2 years were more frequently affected than young. The occurrence of epithelial tumors (60/66) was greater than the mesenchymal tumors (6/66). The most common epithelial tumors were ocular SCC and cutaneous papilloma. Number of malignant tumors (41/66) was higher than number of benign tumors (25/66). Variable degrees of bovine SCC differentiation were detected. Meanwhile ovine SCC appeared only well differentiated.

A survey of tumors affecting cattle, buffaloes and sheep, in El-Dakahlyia Governorate

In a 2-year survey of tumors occurring in ruminants reared in El-Dakahlyia Governorate, Egypt during the period from October 2016 to October 2018. 66 tumor masses were collected: 60 from cattle, 2 from buffaloes and 4 from sheep. According to histopathology of cattle tumors, 15 neoplasms were diagnosed as cutaneous papilloma, 5 neoplasms were diagnosed as cutaneous fibropapillomas, 25 neoplasms were diagnosed as squamous-cell carcinoma (SCC) of the eye; 8 neoplasms were diagnosed as SCC of perineum, 1 neoplasm was diagnosed as SCC in skin of muzzle, 2 neoplasms were diagnosed as epulis in dental pad, 2 neoplasms were diagnosed as vaginal leiomyoma, 1 neoplasm was diagnosed as fibroma and 1 neoplasm was diagnosed as liposarcoma. Histopathology of buffalo tumors revealed SCC in head skin (1 case) and buccal cavity (1 case). Histopathology of sheep tumors revealed SCC in skin under the tail (2 cases), skin of groin (1 case) and skin of abdomen (1 case). In conclusion, cattle (60/66) were more susceptible to tumors than buffaloes (2/66) and sheep (4/66). Females accounted for a higher percentage of the neoplasms (47/66) than males (19/66). Adult animals aged above 2 years were more frequently affected than young. The occurrence of epithelial tumors (60/66) was greater than the mesenchymal tumors (6/66). The most common epithelial tumors were ocular SCC and cutaneous papilloma. Number of malignant tumors (41/66) was higher than number of benign tumors (25/66). Variable degrees of bovine SCC differentiation were detected. Meanwhile ovine SCC appeared only well differentiated.

“Liquid biopsy” for brain tumors: state of problem

The possibilities of traditional methods of diagnosis (radiological and morphological) of brain tumors are now almost exhausted. With their availability and visibility, they have a number of drawbacks in the form of risks of subjectivity in the evaluation of images and microscopic pictures, limited capabilities of existing equipment, the need to use invasive techniques to obtain material. In addition, they do not meet the requirements for individualization of treatment methods, which becomes available as knowledge about the molecular genetic characteristics of tumors deepens. Developed in recent years, the method of “liquid biopsy”, based on the definition in the biological fluids of cells or other components of the tumor has shown its informative in a number of malignant tumors of internal organs. With its help, it is possible to identify the genotype of the tumor and on this basis to individualize the treatment process, as well as to evaluate its effectiveness. The process of finding methods and developing techniques for noninvasive diagnosis of refined genotypes of brain tumors is currently under development. By identifying tumorspecific markers in peripheral blood and cerebrospinal fluid, it is already possible to identify the presence and condition of IDH1 and MGMT genes that are critical for gliomas and to start solving the problem of individualization of therapy.

The overexpression of IGF-1 is a poor prognostic factor in multiple myeloma

Introduction . Insulin-like growth factors (IGF) are one of the widely studied factors in oncology. For tumors with a high expression level of IGF typical postoperative relapse, they are invasive and give distant metastases. There are also data on the participation of IGF in the emergence of resistance to anticancer drugs. The mechanisms that determine the influence of insulin-like growth factors on the progression of a number of malignant neoplasms remain undisclosed and carrying out fundamental research in this direction is relevant. Objective: to study the role of IGF type 1 (IGF-1) in multiple myeloma (MM).Materials and methods . 26 samples of bone marrow aspirates received from 26 patients – 14 men and 12 women – were studied in the work. All patients were diagnosed with stage III ММ. The age of patients ranged from 52 to 72 years. From the obtained bone marrow aspirates, using centrifugation in the Ficoll gradient, a mononuclear fraction of bone marrow cells containing plasma cells was obtained. Then we carried out the procedure of extracting RNA and using polymerase chain reaction with reverse transcription, we studied the expression of mRNA of the genes of IGF-1 and MDR1/ABCB1.Results . The paper analyzes the overall survival (OS) of patients with MM depending on the expression of the gene IGF-1. It is shown that for patients with MM who have a high level of IGF-1 expression, a decrease in OS is characteristic and, conversely, with a weak expression of IGF-1 or in the absence of its expression, an increase in OS is observed. Studies of expression of IGF-1 gene and MDR1/ABCB1 gene responsible for the occurrence of multiple drug resistance showed that these genes are co-expressed in patients with MM. Conclusion . The obtained results indicate that the high level of IGF-1 gene expression may be a poor prognostic factor in ММ. IGF-1 may participate in regulation of the mechanisms of emergence of multiple drug resistance in patients with MM.