Limited Number Research Articles

Long-term Kidney Transplant Survival Across the Globe.

The outcomes after kidney transplantation (KT), including access, wait time, and other issues around the globe, have been studied. However, issues do vary from one country to another. We obtained data from several countries from North America, South America, Europe, Asia, and Australia, including the number of patients awaiting KT from 2015, transplant rate per million population (pmp), proportion of living donor and deceased donor (LD/DD) KT, and posttransplant survival. We also sought opinions on key difficulties faced by each of these countries with respect to KT and long-term survival. Variation in access to KT across the globe was noted. Countries with the highest rates of KT pmp included the United States (79%) and Spain (71%). A higher proportion of LD transplants was noted in Japan (93%), India (85%), Singapore (63%), and South Korea (63%). A higher proportion of DD KTs was noted in Spain (90%), Brazil (90%), France (85%), Italy (85%), Finland (85%), Australia-New Zealand (80%), and the United States (77%). The 5-y graft survival for LD was highest in South Korea (95%), Singapore (94%), Italy (93%), Finland (93%), and Japan (93%), whereas for DD, it was South Korea (93%), Italy (88%), Japan (86%), and Singapore (86%). The common issues surrounding KTs are access and a limited number of LDs and DDs. Key issues identified for long-term survival were increasing age of donors and recipients, higher recipient comorbidity, and posttransplant events, such as alloimmune injury to the kidney, infection, cancer, and suboptimal adherence to therapy. A unified approach is necessary to improve issues surrounding KT as the demand continues to increase.

Use of electrical stimulation for accelerated orthodontics in humans: a systematic review and meta-analysis.

The main objective of this systematic review is to comprehensively evaluate all the relevant studies to evaluate the effectiveness of electrical stimulation to accelerate orthodontic tooth movement in human patients. Using a suitable search strategy, various databases like PubMed, CENTRAL, Scopus and Google Scholar were surveyed for relevant randomized controlled trials (RCTs); after which a hand search of related orthodontic journals was completed. The risk of bias of studies was checked using Cochrane's ROB-2 tool. The quality of evidence of the included studies was evaluated using the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) framework. Rate of orthodontic tooth movement was compared and thus efficiency of electrical stimulation to accelerate tooth movement was assessed. Meta-analysis was done on 2 studies out of the 4; rate of orthodontic tooth movement was compared at two time periods after start of treatment (3rd month and 5th month) with standardized mean difference (SMD) as summary estimate, with p < 0.05 denoting statistical significance. Publication bias was checked using a funnel plot. Via exploration of the online databases and hand searching,1080 studies were found after removing duplicates. After full text assessment, 13 studies in total were selected that used electrical stimulation, out of which 4 studies were included in the qualitative analysis as they met the inclusion criteria. The meta-analysis was done for 3rd month and 5th month with SMD as 0.69(-1.26-0.12) and 1.64(-3.44-0.16), respectively. The strength of evidence in the meta-analysis is moderate. There was no publication bias as shown by funnel plot. The studies included showed increased risk of selection and performance bias. Also due to the limited number of studies available, there was difficulty in reaching definitive conclusions. The meta-analysis showed a significant difference among the control and experimental group for the 3rd month. Even though a positive correlation is found between electrical stimulation and acceleration of orthodontic tooth movement, it is advised to conduct further studies, particularly with human participants, to establish a more conclusive outcome. This review has received no funding. The protocol for the study was registered in the Prospective Registration of Systematic Review (PROSPERO)(CRD42023495077).

Pediatric trauma volume fell during the initial COVID-19 wave but rebounded to new highs for the remainder of 2020.

Previous studies have shown a reduction in pediatric trauma volume during COVID-19, but many have looked at a limited number of facilities, analyzed a narrow timeframe, or both. The objective of this analysis was to assess the impact of COVID-19 on pediatric trauma volume for a statewide sample during 2020. Based on previous literature, researchers hypothesized a reduction in volume during the implementation of these policies. Retrospective cross-sectional analysis of five years (2016 - 2020) of Indiana's statewide trauma patient registry. Patients under age 15 were included. Those who were transfer patients or missing key data were excluded. In total, 10,926 patients were included in analysis. Baseline years (2016 - 2019) were compared to 2020 to estimate the impact of COVID-19 on pediatric trauma volume. Overall monthly volume of pediatric traumas were lower than baseline in March and April 2020 (though not significantly), but rebounded quickly and were above trend in the latter half of the year. Injury patterns differed in both mechanism and location from previous years. Gunshot wounds were more prevalent than previous years, while the volume of non-accidental traumas fell slightly. Injuries that occurred in private residences rose significantly, while fewer took place in schools. Results indicated an initial drop in injury volume consistent with previous findings, but these were offset by increased volume in the second half of 2020. The growth in gun violence is concerning and warrants additional research. Changes in behavior in response to the pandemic such as reduced participation in sports and use of playgrounds, reduced driving, and increased time at home help explain the changes observed in injury patterns. These findings emphasize the continued need for pediatric trauma care during the pandemic.

Methylene Blue in Metformin Intoxication: Not Just Rescue But Also Initial Treatment.

Metformin (MTF) is a widely used oral antidiabetic medication. Regardless the reason, high doses of MTF cause lactic acidosis as a result of its effects on mitochondrial ATP production and no-mediated vascular smooth muscle relaxation. Metformin-associated lactic acidosis can be life-threatening despite all treatments. Methylene blue (MB) has the potential to reverse the toxic effects of MTF through its effects on both the mitochondrial respiratory chain and nitric oxide production. The use of MB in MTF intoxication has only been reported in a limited number of cases. Herein, we present a 16-year-old female patient who attempted suicide by ingesting high doses of MTF. Supportive treatments, such as vasopressor, inotropic treatments, and sodium bicarbonate, were started in the patient who developed fluid-resistant hypotension after pediatric intensive care unit admission. Because of rising lactate levels, Continuous renal replacement therapy (CRRT) was started immediately. Despite all treatments, hypotension and hyperlactatemia persisted; MB was given as a rescue therapy. Noticeable hemodynamic improvement was observed within 30 minutes of initiating MB infusion, allowing a gradual decrease in the doses of inotropic infusions within the first hour of therapy. Patient's cardiovascular support was discontinued on the second day, and she was discharged on the fifth day. We speculate that, considering the mechanisms of MTF toxicity and the mechanisms of action of MB, it is suggested that early administration of MB, not only as a rescue treatment but as the initial approach to MTF poisoning in combination with other treatments, may result in improved outcomes.

Borreliella burgdorferi factor H-binding proteins are not required for serum resistance and infection in mammals.

The causative agent of Lyme disease (LD), Borreliella burgdorferi, binds factor H (FH) and other complement regulatory proteins to its surface. B. burgdorferi B31 (type strain) encodes five FH-binding proteins (FHBPs): CspZ, CspA, and the OspE paralogs OspEBBN38, OspEBBL39, and OspEBBP38. This study assessed potential correlations between the production of individual FHBPs, FH-binding ability, and serum resistance using a panel of infectious B. burgdorferi clonal populations recovered from dogs. FHBP production was assessed in cultivated spirochetes and by antibody responses in naturally infected humans, dogs, and eastern coyotes (wild canids). FH binding specificity and sensitivity to dog and human serum were also assessed and compared. No correlation was observed between the production of individual FHBPs and FH binding with serum resistance, and CspA was determined to not be produced in animals. Notably, one or more clones isolated from dogs lacked CspZ or the OspE proteins (a finding confirmed by genome sequence determination) and did not bind FH derived from canines. The data presented do not support a correlation between FH binding and the production of individual FHBPs with serum resistance and infectivity. In addition, the limited number and polymorphic nature of cp32s in B. burgdorferi clone DRI85A that were identified through genome sequencing suggest no strict requirement for a defined set of these replicons for infectivity. This study reveals that the immune evasion mechanisms employed by B. burgdorferi are diverse, complex, and yet to be fully defined.

Neuropsychological longitudinal study of patients with low-grade gliomas: Cognitive impairment

This study is part of a longitudinal research program, in which patients diagnosed with low-grade gliomas (LGG: n = 13), as well as healthy controls (n = 13), were consecutively recruited and neuropsychologically followed for 7 years. The patients are followed up regardless of variations in treatment. A composite score is used (Global Deficit Score: GDS) included cognitive measures where at least five patients had a negative change: information processing speed, speed of naming, construction ability, verbal fluency, non-verbal thinking, and immediate non-verbal memory. The most important finding in this 7-year follow-up study is that two-thirds of the patients developed cognitive impairment. The remaining third of the patients showed stability in their cognitive ability and were still alive 17 years after diagnosis. Younger patients with tumors in the right frontal or posterior regions showed a more favorable development. Patients with frontal tumors and a declined GDS show also significant changes in executive functions. Given the limited number, no firm conclusions can be drawn regarding the impact of tumor localization. The impact of LGG on cognition and the survival time after diagnosis varies considerably between patients. However, most of the patients (69%) showed cognitive impairment during the seven years we followed them.

A New Macro-Element for Predicting the Behavior of Masonry Structures under In-Plane Cyclic Loading

A new macro model for the finite element modeling of unreinforced masonry (URM) exhibiting in-plane nonlinear cyclic behavior is proposed. The ultimate objective is to predict the seismic response of multi-story URM buildings. The macro model enables the modeling of URM shear walls with a limited number of degrees of freedom (DOF) at low computation times. The macro model consists of a deformable elastic frame supported by diagonal struts with nonlinear behavior aiming to capture all dissipative phenomena occurring during seismic events. The nonlinear constitutive behavior of diagonal struts is inspired by models documented in the literature, ensuring a robust foundation for the proposed approach. This paper first provides a comprehensive review of the principal models currently available for URM analysis. It then articulates the rationale behind the development of this new numerical model, aiming to address the limitations encountered in existing methodologies and to offer a simple and fast tool for predicting the seismic behavior of URM buildings. Afterward, the new model is presented and tested with the simulations of two experimental campaigns performed on different URM walls. The comparison between experimental and numerical results shows that with a limited number of DOF and parameters, it is possible to obtain a prediction of the experimental results with satisfying accuracy.

Subscription Pricing for Free Delivery Services

Many firms that offer home delivery of products purchased online now provide consumers the option of subscribing to a membership plan for free delivery of limited or unlimited number of orders instead of customers paying delivery charges for each order. This paper seeks to understand the benefits to firms and consumers of introducing such free-delivery subscription (FDS) plans to complement their traditional pay-for-delivery (PFD) option, characterize the firm's optimal FDS subscription pricing strategy, and study the effects of product, consumer, and market characteristics on the subscription fees and firm's profits. We develop and analyze an economic model that incorporates salient features of product delivery settings that prior models of subscription pricing do not fully capture. The features include simultaneous availability of PFD and FDS options, lift in a consumer's demand when she switches from PFD to FDS, the possibility of attracting new consumers when the firm introduces FDS, and order batching by customers due to per-order delivery charges and free-delivery order allowances. Our utility-based approach to model consumers’ purchasing choices incorporates consumer heterogeneity both in terms of their utility for the product and their shopping preference under the PFD option. For homogeneous consumers, we show that introducing a FDS plan raises the firm's profit, establish the optimality of offering unlimited free-delivery allowance, and analyze comparative statics. With multiple customer types, our analysis of two subscription pricing strategies for FDS plans—a single “Universal” plan and multiple “Tiered” plans with varying subscription fees and free-delivery limits—leads to several interesting insights on delivery pricing strategies, as well as drivers and sensitivity of the optimal policy, profits, and number of subscribers. We supplement this analysis with extensive computational experiments that reveal a substantial increase in profits when a firm adds one or more FDS plans to its PFD option even though the optimal subscription fee(s) only covers part of the firm's actual shipping costs. These results also permit us to gauge the influence and understand the trends in the profitability of the Universal and Tiered plans as various parameters change.

Alkaloid chemistry in pill-millipedes: Defensive secretion in two species of Typhloglomeris Verhoeff, 1898 (Diplopoda, Glomerida, Glomeridellidae)

AbstractMillipedes of the order Glomerida are known to produce quinazolinone alkaloids as defensive substances. However, chemical data are rather scarce and all hitherto available publications refer to a single glomeridan family, the Glomeridae. To contribute to the knowledge of the chemical ecology of glomeridans, we collected species, Typhloglomeris coeca Verhoeff, 1898 and T. varunae Makarov, Lučić, Tomić &amp; Karaman, 2003, from a second family – Glomeridellidae. The defensive exudates were extracted in methylene chloride and analyzed by gas chromatography-mass spectrometry. Our results show that the secretions of the two glomeridellids contain the quinazolinone alkaloid homoglomerin (2-ethyl-1-methylquinazolin-4(1H)-one) as the sole defensive constituent. This report provides initial data on the chemoprofiles of members of the family Glomeridellidae and supports the phenomenon of chemical homogeneity of glomeridan defensive secretions. So far, all analyzed representatives of the order possess glomerin and/or homoglomerin as defensive equipment. The phylogenetic origin of alkaloidal compounds in the secretions of millipedes in general, and particularly the origin of quinazolinone alkaloids in Glomerida is discussed. Considering that the quinazolinone alkaloid defensive secretions may be the same (or very similar) in other Glomerida, our results have important implication for further studies of defensive secretions in these arthropods. Also, as data are available only for a limited number of glomeridan and colobognathan species, additional analyses are necessary to elucidate the evolution of alkaloid defensive secretion in Diplopoda.

The role of the pulmonary veins on left atrial flow patterns and thrombus formation.

Atrial fibrillation (AF) is the most common human arrhythmia, forming thrombi mostly in the left atrial appendage (LAA). However, the relation between LAA morphology, blood patterns and clot formation is not yet fully understood. Furthermore, the impact of anatomical structures like the pulmonary veins (PVs) have not been thoroughly studied due to data acquisition difficulties. In-silico studies with flow simulations provide a detailed analysis of blood flow patterns under different boundary conditions, but a limited number of cases have been reported in the literature. To address these gaps, we investigated the influence of PVs on LA blood flow patterns and thrombus formation risk through computational fluid dynamics simulations conducted on a sizeable cohort of 130 patients, establishing the largest cohort of patient-specific LA fluid simulations reported to date. The investigation encompassed an in-depth analysis of several parameters, including pulmonary vein orientation (e.g., angles) and configuration (e.g., number), LAA and LA volumes as well as their ratio, flow, and mass-less particles. Our findings highlight the total number of particles within the LAA as a key parameter for distinguishing between the thrombus and non-thrombus groups. Moreover, the angles between the different PVs play an important role to determine the flow going inside the LAA and consequently the risk of thrombus formation. The alignment between the LAA and the main direction of the left superior pulmonary vein, or the position of the right pulmonary vein when it exhibits greater inclination, had an impact to distinguish the control group vs. the thrombus group. These insights shed light on the intricate relationship between PV configuration, LAA morphology, and thrombus formation, underscoring the importance of comprehensive blood flow pattern analyses.

Reporting a Cyber Security Breach: How Organizations Respond

Cyber breaches and ransomware attacks now occur so frequently that they have become facets of organizational life. These breaches are unique in that they are initially silent; a limited number of organizational members know about the incident so firms usually have ample time to prepare a Cyber Breach Revelation (CBR). This study analyzes 378 press releases acknowledging a cyber security breach. It finds four response clusters that highlight how compromised organizations reveal cyberattacks to external stakeholders: (1) empathetic acknowledgment; (2) assurance of the organization’s breach-handling capability; (3) restoration of confidence; and, (4) re-establishment of trust and loyalty. These clusters provide meaningful insights as to how firms reveal cyber breaches to their stakeholders and, equally as important, open the door for further studies as to the effectiveness and sequencing of these approaches.

Acquired immune resistance is associated with interferon signature and modulation of KLF6/c-MYB transcription factors in myeloid leukemia.

Resistance to immunity is associated with the selection of cancer cells with superior capacities to survive inflammatory reactions. Here, we tailored an ex vivo immune selection model for acute myeloid leukemia (AML) and isolated the residual subpopulations as "immune-experienced" AML (ieAML) cells. We confirmed that upon surviving the immune reactions, the malignant blasts frequently decelerated proliferation, displayed features of myeloid differentiation and activation, and lost immunogenicity. Transcriptomic analyses revealed a limited number of commonly altered pathways and differentially expressed genes in all ieAML cells derived from distinct parental cell lines. Molecular signatures predominantly associated with interferon and inflammatory cytokine signaling were enriched in the AML cells resisting the T-cell-mediated immune reactions. Moreover, the expression and nuclear localization of the transcription factors c-MYB and KLF6 were noted as the putative markers for immune resistance and identified in subpopulations of AML blasts in the patients' bone marrow aspirates. The immune modulatory capacities of ieAML cells lasted for a restricted period when the immune selection pressure was omitted. In conclusion, myeloid leukemia cells harbor subpopulations that can adapt to the harsh conditions established by immune reactions, and a previous "immune experience" is marked with IFN signature and may pave the way for susceptibility to immune intervention therapies.

Attention Autism™ training for occupational therapists: a pilot study

Purpose At present, there is no reference to Attention Autism (AA) as a framework and therapeutic tool with autistic children in occupational therapy (OT) literature. By way of introducing AA as a potential intervention to the OT community, this study aims to investigate the extent to which participation in a two-day AA training could contribute to increasing confidence and inspire changes in practice for Irish occupational therapists (OTs) supporting autistic children. Design/methodology/approach A pilot study design with mixed qualitative and quantitative methods was used to evaluate the impact of a two-day AA training on six OTs. The OTs support autistic children throughout Ireland across public, private and voluntary sectors. They completed brief, non-standardised questionnaires 2 weeks before the training (Time 1) and again 12 weeks post (Time 2) training session. At Time 2, additional exploratory questions were answered by OTs regarding their use of AA in practice. Findings This explorative study’s quantitative findings presented percentage change increases within three areas of confidence for all OTs. These include establishing attention, motivating and developing functional skill goals with autistic children. One of the participants did not score any change in confidence in a fourth area, building rapport, however, the five other participants scored percentage change increases. Qualitative data provided by participants showed that they were implementing AA in practice since attending the training. Five of the participants reported positive experiences of using AA and one participant reported the programme was not suitable for her caseload because of their level of understanding and need. Research limitations/implications This was a small, exploratory, practice-based study. As this is the first study exploring this area of practice for OTs, to the best of the authors’ knowledge, there were no standardised methods of assessment available, therefore a self-designed survey was used by the author which had a limited number of open-ended questions and four Likert scale questions. This study was also limited in that there was one main researcher who also delivered the two-day AA training. The sample data set was small which resulted in the limitation of the choice of methods used to analyse the quantitative data. Percentage changes were used as the only available and reliable method for a small data set. Originality/value Findings of this study, despite their preliminary nature, indicate that AA training may be a useful professional development consideration for OTs who provide a service for autistic children. Further AA research in OT is required including larger and more rigorous studies. An alternative training option of The Curiosity Programme may be considered for OTs supporting children who may not yet be ready to participate in AA.

Upregulated expression of lamin B receptor increases cell proliferation and suppresses genomic instability: implications for cellular immortalization.

Mammalian somatic cells undergo terminal proliferation arrest after a limited number of cell divisions, a phenomenon termed cellular senescence. However, cells acquire the ability to proliferate infinitely (cellular immortalization) through multiple genetic alterations. Inactivation of tumor suppressor genes such as p53, RB and p16 is important for cellular immortalization, although additional molecular alterations are required for cellular immortalization to occur. Here, we aimed to gain insights into these molecular alterations. Given that cellular immortalization is the escape of cells from cellular senescence, genes that regulate cellular senescence are likely to be involved in cellular immortalization. Because senescent cells show altered heterochromatin organization, we investigated the implications of lamin A/C, lamin B1 and lamin B receptor (LBR), which regulate heterochromatin organization, in cellular immortalization. We employed human immortalized cell lines, KMST-6 and SUSM-1, and found that expression of LBR was upregulated upon cellular immortalization and downregulated upon cellular senescence. In addition, knockdown of LBR induced cellular senescence with altered chromatin configuration. Additionally, enforced expression of LBR increased cell proliferation likely through suppression of genome instability in human primary fibroblasts that expressed the simian virus 40 large T antigen (TAg), which inactivates p53 and RB. Furthermore, expression of TAg or knockdown of p53 led to upregulated LBR expression. These observations suggested that expression of LBR might be upregulated to suppress genome instability in TAg-expressing cells, and, consequently, its upregulated expression assisted the proliferation of TAg-expressing cells (i.e. p53/RB-defective cells). Our findings suggest a crucial role for LBR in the process of cellular immortalization.

Antifungal drug resistance in Candida: a special emphasis on amphotericin B.

Invasive fungal infections in humans caused by several Candida species, increased considerably in immunocompromised or critically ill patients, resulting in substantial morbidity and mortality. Candida albicans is the most prevalent species, although the frequency of these organisms varies greatly according to geographic region. Infections with C. albicans and non-albicans Candida species have become more common, especially in the past 20 years, as a result of aging, immunosuppressive medication use, endocrine disorders, malnourishment, extended use of medical equipment, and an increase in immunogenic diseases. Despite C. albicans being the species most frequently associated with human infections, C. glabrata, C. parapsilosis, C. tropicalis, and C. krusei also have been identified. Several antifungal drugs with different modes of action are approved for use in clinical settings to treat fungal infections. However, due to the common eukaryotic structure of humans and fungi, only a limited number of antifungal drugs are available for therapeutic use. Furthermore, drug resistance in Candida species has emerged as a result of the growing use of currently available antifungal drugs against fungal infections. Amphotericin B (AmB), a polyene class of antifungal drugs, is mainly used for the treatment of serious systemic fungal infections. AmB interacts with fungal plasma membrane ergosterol, triggering cellular ion leakage via pore formation, or extracting the ergosterol from the plasma membrane inducing cellular death. AmB resistance is primarily caused by changes in the content or structure of ergosterol. This review summarizes the antifungal drug resistance exhibited by Candida species, with a special focus on AmB.

An Expert System for Indian Sign Language Recognition Using Spatial Attention–based Feature and Temporal Feature

Sign Language (SL) is the only means of communication for the hearing-impaired people. Normal people have difficulty understanding SL, resulting in a communication barrier between hearing impaired people and hearing community. However, the Sign Language Recognition System (SLRS) has helped to bridge the communication gap. Many SLRs are proposed for recognizing SL; however, a limited number of works are reported for Indian Sign Language (ISL). Most of the existing SLRS focus on global features other than the Region of Interest (ROI). Focusing more on the hand region and extracting local features from the ROI improves system accuracy. The attention mechanism is a widely used technique for emphasizing the ROI. However, only a few SLRS used the attention method. They employed the Convolution Block Attention Module and temporal attention but Spatial Attention (SA) is not utilized in previous SLRS. Therefore, a novel SA based SLRS named Spatial Attention-based Sign Language Recognition Module (SASLRM) is proposed to recognize ISL words for emergency situations. SASLRM recognizes ISL words by combining convolution features from a pretrained VGG-19 model and attention features from a SA module. The proposed model accomplished an average accuracy of 95.627% on the ISL dataset. The proposed SASLRM is further validated on LSA64, WLASL, and Cambridge Hand Gesture Recognition datasets where, the proposed model reached an accuracy of 97.84%, 98.86%, and 98.22%, respectively. The results indicate the effectiveness of the proposed SLRS in comparison with the existing SLRS.

Triple Problems of Nepalese Hills: Low Income, Migration and, Land Use Changes – One Possible Solution: Specialty Rice

The hills of Nepal face a complex triad of interrelated challenges: low income, outmigration, and land abandonment leading to aquifer desiccation, with low income serving as the precursor of this vicious cycle. The Nepalese hills harbor two significant yet underutilized potentials. First, the picturesque terraces, a legacy of preceding generations, are unfortunately abandoned and neglected. Second, these hills harbor diverse landrace varieties of rice, each with unique traits, though only a limited number among them have received the deserved recognition and fair market prices. This study seeks to identify and analyze these rice varieties, investigate their value chain, and conduct a comparative cost-benefit analysis against other rice varieties across Nepal to evaluate their economic feasibility. This study, conducted across 12 districts in Eastern Nepal, involved 12 focused group discussions with rice farmers, 200 individual interviews with rice farmers, as well as 10 individual interviews each with rice millers, agro vets, and rice traders. The research employed cost-benefit analysis as a tool to assess economic feasibility. The findings indicated that some of these rice varieties demonstrate a benefit-to-cost ratio ranging from two to four times higher than conventional rice varieties. The economic viability of most other varieties remains unknown as they have not yet been tested in the market. These findings present crucial insights for researchers, policymakers, development practitioners, and the private sector to further explore, act upon, facilitate, and invest in the untapped potential, aiming to break the vicious cycle and ensure increased income, reduced migration, and conservation of ecological and environmental resources.

Achieving White Light Emission with High Luminescence Efficiency via Combustion Produced Sr3Y(PO4)3: Dy3+ Nanophosphors for Photonic Applications.

Extensive investigations were conducted on the structural and photoluminescence characteristics of the present nanosamples, encompassing PL, TEM, PXRD, EDAX, CCT, and CIE research. PXRD studies established a single phase, and TEM instruments were used to examine the dimensions and topographical behavior. The EDAX analysis examined the magnitude of the different components that were present. Decay lifetimes, radiative and non-radiative energy transfer rates, and a number of intensity limitations have all been found using PL spectra. Two significant peaks were visible in the blue (B) and yellow (Y) regions of the photoluminescence (PL) spectra upon NUV excitation, at 486nm and 577nm. At 7mol% Dy3+ ions, the PL intensity peaked. After that, it began to decline as a result of the concentration quenching process brought on by multipolar exchanges (s = 4.1445). The values of 0.86423ms, 81%, and 226s-1 were discovered to be the decay life time, non radiative rates, and quantum efficiency of the ideal powder, respectively. Further analysis of Sr3Y0.93Dy0.07(PO4)3 nanocrystals revealed that their chromaticity coordinates (0.305, 0.321), and CCT value (6902K) matched those of NTSE and commercial LEDs, certifying their use in innovative optoelectronic appliances, particularly single phased WLEDs.

Business model innovation and Industry 5.0: a possible integration in GLAM institutions

PurposeThis research delves into the realm of Business Model Innovation (BMI), integrating it with the human-centric, sustainable, and resilient principles of Industry 5.0, proposing a new theoretical framework.Design/methodology/approachAn abductive approach has been chosen to expand existing knowledge developing new ideas based on emerging phenomena. Data were gathered via semi-structured interviews with directors, managers and curators of public institutions in Italy, Switzerland, Germany and Spain encompassing Galleries, Libraries, Archives, and Museums (GLAM). These data were subsequently subjected to thematic analysis.FindingsThe findings indicate that the main enablers for Business Model Innovation (BMI) in combination with Industry 5.0 encompassed stakeholder, customer and organizational engagement, collaborative environment, knowledge and innovation management, and sustainability. These drivers were effectively leveraged through three pivotal facilitators-inhibitors: technology, resources, and leadership.Research limitations/implicationsThe principal constraints are rooted in the narrow contextual focus and the limited participants number. However, upcoming research efforts may broaden the horizons of this multifaceted and extensive investigation.Originality/valueThis study is groundbreaking as it fills a significant gap in the existing literature by integrating Business Model Innovation (BMI) with the Industry 5.0 paradigm, a novel approach that has not been explored previously. Additionally, the inclusion of GLAM institutions in this research adds a unique dimension, as they have been largely overlooked in both research domains.

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection.

In recent years, the importance of isolating single cells from blood circulation for several applications, such as non-invasive tumour diagnosis, the monitoring of minimal residual disease, and the analysis of circulating fetal cells for prenatal diagnosis, urged the need to set up innovative methods. For such applications, different methods were developed. All show some weaknesses, especially a limited sensitivity, and specificity. Here we present a new method for isolating a single or a limited number of cells adhered to SBS slides (Tethis S.p.a.) (a glass slide coated with Nanostructured Titanium Dioxide) by Laser Capture Microdissection (LCM) and subsequent Whole Genome Amplification. SBS slides have been shown to have an optimal performance in immobilizing circulating tumour cells (CTCs) from early breast cancer patients. In this work, we spiked cancer cells in blood samples to mimic CTCs. By defining laser parameters to cut intact samples, we were able to isolate genetically intact single cells. We demonstrate that SBS slides are optimally suited for isolating cells using LCM and that this method provides high-quality DNA, ideal for gene-specific assays such as PCR and Sanger sequencing for mutation analysis.