Number Of Lateral Shoots Research Articles

Analysis of the Impact of Treatments Stimulating Branching on the Quality of Maiden Apple Trees

Nursery material intended for establishing intensive apple orchards should be characterised by a dominant and straight leader with an appropriate number of shoots that develop at the right height and are regularly spaced along the leader. The use of well-branched trees can lead to fruiting in the first year after planting. However, many apple varieties have difficulty forming lateral shoots due to strong apical dominance. The aim of the study was to assess the effectiveness of treatments stimulating the branching of maiden apple trees of the ‘Gloster’ variety. The research was carried out in 2017–2019 at a private nursery farm located in eastern Poland. The studied trees were subjected to a mechanical branching stimulation treatment, which consisted of pinching off 4–5 of the youngest leaves located below the growth cone, and chemical branching stimulation treatments, which consisted of applying growth regulator mixtures in the form of an aqueous solution, i.e., BA+GA3 and BA+GA4+7. The conducted studies showed that the type of branching-stimulating treatment had a significant effect on the height and trunk diameter of the maiden trees, the number of lateral shoots, the average length of one shoot and the sum of the lengths of all sylleptic shoots. The maiden trees treated with BA+GA3 were characterised by the best quality among the analysed combinations. Maiden apple trees treated with BA+GA3 were the tallest (2017—167.7 cm; 2018—175.3 cm; 2019—164.4 cm), produced the largest number of shoots (2017—6.5 pcs; 2018—6.8 pcs; 2019—6.3 pcs) and had the largest sum of lateral shoot lengths (2017—148.0 cm; 2018—155.4 cm; 2019—140.6 cm) among the evaluated combinations. The number of treatments and the concentration of applied growth regulators had a significant effect on the structure of the crown of the maiden apple trees of the ‘Gloster’ cultivar.

Study of the toxicity of a corrosion inhibitor for macrophytes

This article presents the results of studies of the toxicity of a corrosion inhibitor used to treat oilfield equipment in relation to a representative of submerged aquatic plants – Elodea canadensis. The characteristics of the toxicant and research methods are given in detail. The method of mathematical processing is indicated. The work presents the results of studying the toxicity of the substance in a wide range of concentrations: from 100 to 5000 mg/dm³ (4 days – acute experience) and 0,05–500,0 mg/dm³ (30 days – chronic experience). During the experiment, changes in the condition and survival of plants, morphological indicators (growth of the main shoot, number of lateral shoots and their length, number of roots and their length, total growth of elodea) were observed. It has been established that the maximum amounts of the substance (5000 and 500 mg/dm³ – acute and chronic experiments, respectively) cause 100% plant death. Lower concentration ranges stimulate morphological parameters. According to the research results, 0,5 mg/l can be taken as a threshold concentration for an inhibitor, and 0,05 mg/l is acceptable.

Growth and yield response of pepper (Capsicum annuum L.) to foliar application of carrageenan plant growth promoter

The study determined the growth and yield response of pepper (Capsicum annuum L.) to varying concentrations and frequencies of Carrageenan Plant Growth Promoter (CPGP). The study was set-up in a split-plot randomized complete block design with three replications. The three varying application frequencies were designated as main-plots, and the four concentrations of CPGP, as sub-plots. Peppers applied with 120 ppm and two to three times application of CPGP significantly matured earlier based on number of days to flowering and number of days to first priming. The same treatment combination resulted in significantly taller plants and higher number of lateral shoots. In terms of yield, however, CPGP at 120 ppm applied three times gave significantly higher number and heavier weight of fruits. The concentration of 120 ppm CPGP applied thrice in growing pepper is shown in the study to be the best treatment combination, thus, is recommended to improve the growth and increase the yield of pepper.

Efficiency of the main application of fertilizers and top dressing of eggplant under drip irrigation on ordinary chernozems of the Rostov region

Relevance. Fertigation is the application of fertilizers, in which nutrients are dissolved in water for irrigation. This method is relevant when growing eggplant in the Rostov region, allowing effective use of fertilizers, since nutrients enter directly into the roots of plants, which eliminates their loss to evaporation or weathering. During fertigation, there is no need to carry out separate procedures for fertilization and irrigation, operations are combined into one, which reduces time and costs. The Rostov region is characterized by variable climatic conditions, including drought and strong winds. Fertigation ensures uniform and constant nutrition of plants, allowing them to better adapt to weather conditions, and can help increase yields by optimizing plant growth and development conditions.Material and methodology. In the conditions of the Rostov region, on ordinary heavy loamy chernozems, studies were conducted on the cultivation of eggplant on different backgrounds of the main application of mineral fertilizers in calculated doses before planting and using root fertilizing with complex fertilizers with trace elements with irrigation (fertigation), as well as leaf fertilizing with organomineral fertilizer Arksoil during the growing season.Results. It was revealed that mineral fertilizers and top dressing accelerated the growth and development of plants, and in the best case, the increase in indicators was 2 times higher compared with the control, while the height of the main stem, the number of lateral shoots and the number of ovaries on the plant, as well as the average weight of the fruit, significantly increased, which ensured an increase in plant productivity. The combined use of basic fertilizer and complex soluble fertilizers in the drip irrigation system has significantly increased plant productivity and increased yields to 77.82 t/ha. The application of high doses of fertilizers in combination with fertigation with water-soluble fertilizers did not actually affect the quality of fruits. The content of dry matter and nitrates remained at an optimal level.

Effects of different shading levels on the growth of Bornmuellera emarginata, a nickel hyperaccumulator for use in agromining

AbstractThe Greek endemic Bornmuellera emarginata, known for its Ni hyperaccumulation capacity, is a promising candidate species for use in agromining. At the same time, the installation of photovoltaic systems on agricultural land has recently been increasing. Field observations that populations of this species usually occupy shaded places under shrubs or trees led us to study the effect of different shading levels on the growth of B. emarginata, with a view to combine the cultivation of the species with photovoltaic panels in the same area. The experiment was conducted under greenhouse conditions and lasted 210 days after sowing (DAS). Seeds were sown directly into 2‐L pots filled with ultramafic soil collected from the field. Three plant samplings (81, 140, and 210 DAS) were conducted during the experiment to determine a set of morphological and physiological parameters of the species (fresh and dry plant biomass, plant height, number of lateral shoots, total number of leaves, proline and chlorophyll concentrations) alongside with its nickel yield at different levels of shading (0%, 20%, 35%, 60%, and 90%). Growth of B. emarginata was only significantly affected at the highest shading level. Nickel yield of the species increased over time and was maximized at the end of the experiment. The highest nickel yield per plant was observed at the intermediate shading level (35%). Bornmuellera emarginata can be considered as a candidate nickel hyperaccumulator for cultivation in ultramafic or nickel‐polluted areas under photovoltaic panels with corresponding shading, providing important environmental benefits.

Influence of plant habitus traits on formation of seed productivity in flax

The studies were carried out at the Institute of Oilseed Crops of the National Academy of Agrarian Sciences of Ukraine in 2019/2020. The material of the study was interspecific hybrids of the second generation obtained in the system of dialel crosses with the participation of two wild annual homostylic species L. angustifolium and L. hispanicum with n = 15 and three accessions of cultivated flax: L 5 (Czech Republic), L 6 (India), M 32/2 (Ukraine). Correlation analysis of habitus traits of a flax plant with the number of bolls was carried out. As a result of studies between the height of the plant and the number of stems on the plant, there is no relationship; between the height of the plant with the number of lateral shoots and the number of bolls, weak and medium correlations, and sometimes their absence, were established (r = +0.218…+0.559; r = +0.253…+0.698, respectively). Medium and strong correlations were found between the number of stems on a plant with the number of side shoots and the number of bolls (r = +0.334…+0.892; r = +0.482…+0.818). Exceptionally strong and stable over the years correlations were observed between the number of lateral shoots on the plant and the number of bolls (r = +0.700…+0.922). The main trait of the habitus of a flax plant, which significantly affects the number of bolls on them, is the number of lateral shoots. This trait can be used as a reliable criterion for selecting plants for breeding for high seed productivity.

How searcher shoots grow and branch in mature liana mats: the case of Merremia boisiana.

The proliferation of vigorous lianas usually forms liana canopies over the crowns of host trees and liana mats on the ground of open areas or large forest gaps. While research on liana canopies has increased significantly in recent decades, our understanding of liana mats remains limited. Merremia boisiana (Convolvulaceae), a fast-growing liana, forms mature liana mats that can persist for decades, characterized by numerous upright searcher shoots that extend from the liana mats in search of supports. However, the reasons behind the proliferation of these searchers, as well as their growth and branching patterns in mature liana mats that lack support, are not well understood. We hypothesize that searchers are an inevitable phase in the growth rhythm of M. boisiana within these mature liana mats. We tested our hypothesis by tracking the lifespan and status of M. boisiana searchers during the early, middle, and late periods of the year. Our findings reveal the following: (1) M. boisiana searchers exhibit apical dominance and have a short lifespan; over 95% of searchers lost their terminal buds within two months during the early period, while it took only one month during the middle and late periods. (2) The original lateral buds of the searchers also have a limited lifespan, yet the nodes can sprout new lateral buds after the loss of their originals. (3) With the withering of terminal buds, the total number of lateral shoots decreased, while the quantity of long lateral shoots (≥ 50 cm) and their elongation rates increased. (4) Each surviving segment of a searcher typically develops one or two long lateral shoots, none of which grow into searchers. We conclude that the shoots of M. boisiana in mature liana mats periodically alternate between searchers and stolons, collectively forming a zigzag branching system. The high rate of lateral bud resprouting may facilitate the rapid recovery of mature liana mats early in the year, while the reduced lifespan of searchers and limited number of long lateral shoots represent effective strategies for M. boisiana, balancing the investment and risk associated with foraging in mature liana mats.

Variations in growth and biochemical parameters in annual cherry plants under the infl uence of growth regulators

This study addresses the influence of the Applin drug on the growth and biochemical parameters of the leaves in three annual cultivars of cherries (Tyutchevka, Podarok Ryazani and Chermashnaya) grafted onto the Izmailovsky rootstock. The research was carried out in 2021-2022 both in field and laboratory conditions at the Federal Horticultural Centre for Breeding, Agrotechnology and Nursery. It was established that following treatment with Applin, the biometric indicators improved, with the yield of branched annual cherry seedlings increasing to 100 %. The treatment and removal of apical leaves stimulated the formation and growth of lateral branches in annual plants. The number of lateral shoots in experimental plants of the Chermashnaya cultivars increased by 6 times, while in the Tyutchevka and Podarok Ryazani cultivars, it increased by 3-4 times relative to the control. Growth regulators induced an increase in the average length of lateral shoots to 30-50 cm in all cultivars. The total length of the branches in the experimental Chermashnaya cultivar was 118.5 cm, which is 6.7 times higher than in the control. The other two cultivars exhibited a 4–4.5 times increase in total growth in treated plants compared with the control. Biochemical studies revealed an increase in photosynthetic pigment content in leaves following Applin treatment. The total content of phenolic compounds and antioxidant activity exhibited significant changes. A positive correlation (r = 0.709) was observed between the antioxidant activity of the aqueous extract and the amount of phenolic compounds. The content of chlorophylls (a+b) in the leaves of treated plants increased by 5-10%. Depending on the cultivar, the content of carotenoids varied from 1.1 to 1.8 mg/g, remaining practically constant following treatment. All cultivars showed an increase in the “chlorophyll (a+b)/carotenoids” ratio. Applin treatment also influenced the synthesis of primary and secondary metabolites, stimulating an increase in the content of carbohydrates (fructose, sucrose, turanose, and trehalose) and phenolic compounds (quinic and chlorogenic acids) in cherry plant leaves

The Evaluation of the Water Consumption and the Productive Parameters of a Table Grapevine, Cardinal Cultivar, Grafted onto Two Rootstocks

This trial was carried out over two years in southern Italy. Two grapevine rootstocks, 110R and SO4, were compared to evaluate their ability to extract water from the soil and the effect on the yield and quality of the Cardinal grapevine table cultivar. Therefore, a new approach to plant water consumption based on sap flow was adopted. The earlier and faster water refilling of the xylem in Cardinal onto 110R (C/110R) appears responsible for the earlier evolution of the phenological phases than Cardinal onto SO4 (C/SO4). The maximum length of the principal shoot was reached in Cardinal/110R compared to the C/SO4, while a higher number of lateral shoots with lower internode changed the canopy architecture and light distribution in the C/SO4. The 110R used more water compared to the SO4. It was possible to quantify the real transpired flux of the plant per day: the sap flow was 12.3 L.plant−1.d−1 and 11.7 L.plant−1.d−1 in C/110R in the first and second year, respectively, while it was 14% lower in the alternative graft combination. The overall lower C/SO4 water status does not compromise the production result, with similar or higher-quality aspects compared to the alternative graft combination C/110R, which can be also attributed to the strong resilience of Cardinal to water deficit.

Effect of Growth Regulators on the Quality of Apple Tree Whorls

High-quality nursery stock ensures the dynamic development of orchard crops. The most desirable by orchardists is a tree with a strongly developed crown, which makes it possible to shorten the investment period of newly planted orchards. The quality of the perianth and the degree of its branching depend on the growth strength of the rootstock, the ability of a given variety to form lateral shoots, the type and effectiveness of the treatments used to stimulate branching, soil and climatic conditions related to the location of the nursery and the course of the weather during the period of initiation and growth of young shoots. The study was conducted in 2017–2019 at a private nursery farm near Lublin. The aim of the experiment was to study the effect of mixtures based on the compounds benzyladenine (BA) and gibberellins (GA3) and (GA4+7) for chemical stimulation of lateral shoot outgrowth in apple cv. ‘Alwa’ and ‘Najdared’. In the course of the research, significant effects of the type of growth regulations used and the variety on the height of the perianths, the number and length of lateral shoots and the degree of branching were demonstrated.

The transcription factor SPL13 mediates strigolactone suppression of shoot branching by inhibiting cytokinin synthesis in Solanum lycopersicum.

Plant architecture imposes a large impact on crop yield. IDEAL PLANT ARCHITECTURE 1 (IPA1), which encodes a SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor, is a target of molecular design for improving grain yield. However, the roles of SPL transcription factors in regulating tomato (Solanum lycopersicum) plant architecture are unclear. Here, we show that the expression of SPL13 is down-regulated in the lateral buds of strigolactone (SL)-deficient ccd mutants and is induced by GR24 (a synthetic analog of SL). Knockout of SPL13 by CRISPR/Cas9 resulted in higher levels of cytokinins (CKs) and transcripts of the CK synthesis gene ISOPENTENYL TRANSFERASES 1 (IPT1) in the stem nodes, and more growth of lateral buds. GR24 suppresses CK synthesis and lateral bud growth in ccd mutants, but is not effective in spl13 mutants. On the other hand, silencing of the IPT1 gene inhibited bud growth of spl13 mutants. Interestingly, SL levels in root extracts and exudates are significantly increased in spl13 mutants. Molecular studies indicated that SPL13 directly represses the transcription of IPT1 and the SL synthesis genes CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7) and MORE AXILLARY GROWTH 1 (MAX1). The results demonstrate that SPL13 acts downstream of SL to suppress lateral bud growth by inhibiting CK synthesis in tomato. Tuning the expression of SPL13 is a potential approach for decreasing the number of lateral shoots in tomato.

GOURD GROWING EFFICIENCY USING SILICON-CONTAINING FERTILIZERS IN THE SOUTH OF UKRAINE

Purpose. To evaluate the effect of pre-sowing soaking of seeds on the plant performance and economic efficiency of watermelon and melon growing on drip irrigation in the South of Ukraine. Methods. Field, measuring, computational, comparative, and mathematical/statistical methods, analysis of economic efficiency. Results. Pre-sowing soaking of seeds in solutions of silicon-containing fertilizers was shown to help obtain early and full-bodied watermelon and melon seedlings, increase the length and number of lateral shoots, and improve fruit setting. Balanced plant nutrition contributed to high performance of watermelon and melon plants in the South of Ukraine. The highest yields of watermelon (23.4 t /ha) and melon (17.5 t/ha) fruits were harvested with 10% Quantum AquaSil solution, which increased the yield by 35.3 and 35.7%, respectively, compared to the untreated control. Silicon-containing fertilizers, 15% Bai-Si and Kelik PotassiumSilicon solutions, increased the fruit yield in watermelon fields by 22.5% and 19.7%, respectively, compared to the untreated control. The greatest economic effect of watermelon and melon growing was achieved by soaking seeds in 10% Quantum AquaSil solution prior to sowing; the profitability amounted to 122 and 101%, respectively. Conclusions. Pre-sowing soaking of seeds in silicon-containing fertilizer solutions contributed to high performance of plants and allowed increasing the economic efficiency of cultivation. The maximum percentage of fruit setting was recorded after seed treatment with 10% Quantum AquaSil solution. For example, this parameter was 67% in watermelon ‘Charivnyk’ and 87% in ‘Didona’ melon. In the south of Ukraine, the economic effect of outdoor watermelon and melon cultivation increased by 16,030–19,220 UAH/ha and 21,230–26,340 UAH/ha, respectively.

A NAC transcription factor ZaNAC93 confers floral initiation, fruit development, and prickle formation in Zanthoxylum armatum

Zanthoxylum armatum is a dioecious prickly plant which developed apomictic reproduction. The increases in male flowers and prickle density in female plants lead to low yield and picking efficiency. However, little is known concerning the mechanisms of floral development and prickle formation. NAC is a well-known transcription factor that participates in multiple aspects of plant growth and development. Herein, we characterize the functions and regulatory mechanisms of candidate NACs controlling both traits in Z. armatum. A total of 159 ZaNACs were identified, and 16 of these were male-biased, represented by the NAP subfamily members ZaNAC93 and ZaNAC34, orthologs of AtNAC025 and AtNARS1/NAC2 respectively. Overexpression of ZaNAC93 in tomato led to modifications in flower and fruit development, including earlier flowering, increased numbers of lateral shoots and flowers, accelerated plant senescence, and reduced size and weight of fruits and seeds. In addition, the trichome density in leaves and inflorescences was dramatically reduced in ZaNAC93-OX lines. Overexpression of ZaNAC93 resulted in the up-/downregulation of genes associated with GA, ABA and JA signaling pathways, such as GAI, PYL and JAZ, as well as several TFs, including bZIP2, AGL11, FBP24 and MYB52. Yeast two-hybrid analysis revealed that ZaNAC93 protein could interact with AP1, GAI, bZIP2 and AGL11 in Z. armatum, which might contribute to floral induction, fruit growth, and trichome initiation. This work provides new insights into the molecular mechanisms of ZaNAC93 in reproductive development and prickle formation in Z. armatum.

NFLUENCE OF GREENHOUSE MAINTENACE TREATMENTS ON GROWTH OF SEEDLING GRAPEVINES (Vitis spp.)

Vegetative clonal propagation of scions and rootstocks represent the commercial method of grapevine production; currently, techniques such as hardwood rooting, grafting, and tissue culture are readily exploited by the nursery and grapevine industries. Seedlings of highly heterozygous grapevines are not true to type, as a result they are not utilized in the mass production of planting stock for commercial grape vineyards. However, seedling growth and quality is key for plant breeding programs initiating thousands of unique seedlings each year. To investigate treatments for their impact on diverse interspecific cold-hardy grapevines, seedlings were grown under greenhouse conditions. Four weeks after transplant, seedlings were cut back to one true node (CUT), leaf thinned (LT), or left as untreated control vines (Control). Eight weeks after treatment, plants were analyzed for total node number, periderm encompassed nodes, lateral shoot number, and total number of base stems. Control and LT vines had more periderm encompassed nodes and total nodes than CUT vines, while CUT vines had the highest number of shoots arising from the base nodes of the vine.

Development of elements of technology for growing branched sweet cherry seedlings

The results of two-year studies on the influence of rootstocks, physiologically active substances and mechanical techniques on biometric indicators of annual seedlings of two varieties of cherries (Tyutchevka and Chermashnaya) are presented. The research was carried out in 2020-2021 in the experimental nursery of the Federal State Budgetary Research Center of Horticulture in the conditions of the Moscow region. The scheme of the experiment included 5 options: I – removal of apical leaves; II – treatment with Apllin (0.6 g/l); III – removal of apical leaves with treatment with Apllin (0.6 g/l); IV – pinching of the tip at a height of 60 cm; st – control, without processing and pinching. The eyepieces were sprayed twice, with an interval of 10 days. It was found that treatment with the preparation Apllin, removal of the apical leaves and their combined use contributed to a significant improvement in the biometric indicators of annual cherry seedlings of weakly branching varieties, increased the number of branches by 2.7-4.6 times and provided in most combinations an increase in the yield of first-class seedlings up to 90-100%. The greatest effect from mechanical and chemical influences was achieved when using the Izmailovsky rootstock. Pinching the top at a height of 60 cm caused a decrease in the linear and radial growth of annuals, an increase in the number of lateral shoots by 1.43 times, but only 50 % of seedlings obtained in this variant corresponded to 1 commercial grade.

Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species.

Citronella mucronata (C. mucronata), a tree species endemic to Chile, has become threatened in its natural habitat and is currently listed as vulnerable. Tree population parameters have deteriorated due to indiscriminate logging and other anthropogenic activities, warranting research on mass propagation as a means of recovery. This study, unprecedented for this native species, has developed a successful method for its micropropagation. The objective was to establish a protocol for in vitro propagation of C. mucronata to produce large quantities of high-quality seedlings in an accelerated plant acquisition process. The best results were achieved by growing explants on Murashige and Skoog (MS) basal culture medium supplemented with 4.44 μM 6-benzylaminopurine (BAP) and 14.76 μM indole-3-butyric acid (IBA). Explant survival rate was 78 %, the average shoot length reached 3.2 cm, the number of lateral shoots was 3.9, and rooting rate was 60%. Furthermore, stimulation with red and blue light in a 1:2 ratio, supplemented with 14.76 μM IBA, improved the rooting rate to 93%. The survival rate of rooted explants reached 100% in the acclimatization stage when using peat and perlite substrate (1:1 v/v).

English

A study was conducted to evaluate the influence of various sowing times on flowering, pollen viability and seed yield of zinnia (Zinnia elegans) cultivars ‘Lilliput᾿ and ‘Super Yoga᾿ under Faisalabad, Pakistan conditions. Seeds of both Zinnia cultivars were sown in 128-cell plug trays at 20 days interval from March 14 to August 20, during 2019. Seedlings were transplanted in thoroughly prepared field beds after 15 days of each sowing, according to factorial arranged randomized complete block design, with three replications. Data were recorded for various growths, flowering traits, pollen viability and seed related parameters. Super Yoga excelled over Lilliput for most the studied traits except number of lateral shoots, flowers per plant, unopened flower buds, pollen viability and seed yield per plant; values of these traits were higher for Lilliput than Super Yoga. Significant variation among various sowing times was recorded in both cultivars. Seeds sown on March 14, 2019 performed best compared to later sowing times for plant height, number of lateral shoots per plant, length of the lateral shoots, leaf area, number of flowers per plant, flower diameter, pollen viability, seed yield per plant and seed viability. However, total number of un-opened flower buds, seed weight per flower and 100-seed weight were more in April 03 sowing, although statistically similar to March 14 sowing. Seed yield declined gradually with delay in sowing; 66% less seed yield in June 20 sowing. Further delay in sowing did not produce seed. It was concluded that Zinnia should be sown by mid-March for getting high seed yield of good quality; later sowing, even during first week of April, resulted in 17.5% decrease in seed yield.

Effect of the exposure period to different water salt levels on the morphological behavior of olive plants

The increasing salinity of water and soil is one of the environmental factors that most threatens the sustainability of olive cultivation systems in the Mediterranean basin. The identification of plant material with high tolerance to this stress would be one of the ways to solve this challenge, but it is generally a slow and expensive process. The selection of the most reliably parameters involved in the response of the plant to salinity and that are easy to evaluate, could help to speed up the identification of the most tolerant genotypes. The objective of this study is to determine the most interesting morphological characters which could be used in future as early criteria in the selection process of olive tolerant genotypes. For that, young plants, three-month-old, were exposed to salinity (0, 4 and 8 g/L NaCl) during different periods of time (30, 50 and 70 days), and several morphological parameters were assessed. The obtained results showed that most of the parameters were affected by the concentration 8 g/L of NaCl after a treatment period of 50 days. The most affected parameters by this level of salinity were the plant height, the leaf number and the number of lateral shoots. The PCA analysis showed that the number of lateral shoots was poorly correlated with the other parameters, but the height and the leaf number were highly correlated with each other.

Micropropagation of the Halophyte Sarcocornia fruticosa (L.) A. J. Scott

Details of investigation to evaluate the effects of the number of nodes (one, two or three) of Sarcocornia fruticosa explants on growth and multiplication rate of plantlets are presented in this paper. The responses of the 3-node explants to some supplementary sources of different aminoacids and growth regulators indol-3-acetic acid, 6-benzylaminopurine and gibberellins A3 were also analysed. Plantlets from 3-node explants showed a marked increase in growth and number of lateral shoots, indicating that Sarcocornia does not respond well when explants are very small. The addition of 100 mg l-1 casein hydrolysate plus 150 mg l-1 glutamine, and 100 mg l-1 casein hydrolysate plus vitamins showed to be good growth promoters in micropropagating Sarcocornia, giving longer plantlets and higher multiplication rates.

Estimation of oil flax intra-species hybrids by breeding-genetic parameters for the creation of material with altered habitus

The creation of a selection material for oil flax with a large number of stems, side shoots and an optimal height for cultivation is an urgent task, since the formation of additional bolls on the side shoots will increase the yield of seeds, and an increase in the vegetative mass of plants will increase its competitiveness against weeds and reduce moisture evaporation. from the soil surface. The use of genetic and statistical parameters allows to efficiently plan and carry out breeding work for oil flax, purposefully select parental pairs in crosses, select valuable genotypes, and reject low-value material at the first stages of breeding. The purpose of this study is to identify promising interspecific hybrid combinations based on breeding and genetic parameters for creating a new breeding material for multi-stem and multi-shoot forms. The studies were carried out at the Institute of Oilseeds NAАS of Ukraine in 2018-2020. The material of the research was F1 and F2 hybrids obtained in a system of dialle crosses with the participation of two wild species (L. angustifolium and L. hispanicum) and three samples of cultivated flax – L 6 (India), M 32/2 (Ukraine), L 5 (Czech Republic). The degree of dominance of traits in hybrids of the first generation was determined by the formula (Beil, Atkins 1965). The degree of heterosis is according to the formula (Rasul et al 2002). The coefficient of heritability in hybrids of the second generation – according to the formula (Ayala 1984). As a result of the research, interspecific hybrid combinations have been identified that are promising for obtaining breeding material with a modified habit: – with the effect of heterosis: by plant height – seven combinations of F1 (Ht = 3,58-13,76%); the number of stems per plant – one (Ht = 35,77%); the number of side shoots is six (Ht = 5,68-43,79%). The most valuable are combinations in which the effect of heterosis manifested itself simultaneously on two grounds – the height of the plants and the number of lateral shoots on the plant – L. angustifolium / L 6, L 5 / L. angustifolium, L. hispanicum / L 6, L 6 / L. hispanicum; plant height and number of stems per plant – M 32/2 / L. hispanicum; – with high coefficients of heritability: by plant height – four combinations of F2 (H = 0,67-0,87); the number of stems per plant – six (H = 0,66-0,81); the number of side shoots is four (H = 0,66-0,72). Interspecific combinations of L. hispanicum / M 32/2 and M 32/2 / L. hispanicum were distinguished by high rates of heritability by two characteristics – the number of stems and the number of lateral shoots on the plant. The potential of the selected combinations will be used in the further breeding process for increased branching.