The potential for increasing soybean productivity and reducing diseases has become evident for Bacillus spp. The objective of this study was to evaluate the efficiency of B. subtilis strain UFMT-Pant001 as a plant growth promoter in soybean under greenhouse conditions. Four independent experiments were conducted in a greenhouse using a B. subtilis UFMT-Pant001 liquid inoculant at 1x109 CFU mL-1. The results showed significant increases in biomass, nodulation, nutrients in the shoot, number of internodes, and root length in soybean cultivars inoculated with B. subtilis UFMT-Pant001 in greenhouse experiments. Positive effects on soybean biomass and other evaluated characteristics were observed with different doses of B. subtilis UFMT-Pant001, with the best results observed when doses of 200 to 300 mL were used for 50 kg of seeds. The use of B. subtilis UFMT-Pant001 did not interfere with soybean nodulation and contributed to an increase in nodule number and weight.
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