Number Of Hydatid Cysts Research Articles

Cystic Echinococcosis and Host Interleukin-2, Interleukin-9 and Interleukin-10 as Biomarkers for Evaluation of Albendazole and Praziquantel Treatment in Experimentally Infected Mice

Echinococcus granulosus sensu lato larvae cause cystic echinococcosis, a highly contagious zoonotic illness that poses a worldwide threat to human health and development. The purpose of this study was to determine the therapeutic effectiveness of albendazole and praziquantel, each alone and in combination, in vivo and to estimate the serum levels of interleukin-2, interleukin-9 and interleukin-10 as biomarkers in evaluating the responsiveness to albendazole and praziquantel treatment in experimentally infected mice. To assess treatment efficacy in vivo, mice were intraperitoneally inoculated with viable protoscolices and then gavaged daily for 1 mo with albendazole (15 mg/kg/d) and praziquantel (25 mg/kg/d), separately and in combination (albendazole 15 mg/kg/d and praziquantel 25 mg/kg/d. The mice were euthanized on d 120 of the experiment and the number, size and location of the generated secondary hydatid cysts were investigated and serum levels of interleukin-2, interleukin-9 and interleukin-10 were estimated. The size and number of hydatid cysts were decreased upon treatment with each drug, but the decrease was significant (p<0.05) in isolates 2, 3, 4, 6, 8, 9 and 10, and 2 and 3, respectively. The most often infected organ was the liver (44.91 %), followed by a peritoneal cavity (30.3 %), subcutaneous (21.1 %), spleen (3.29 %) and lungs (0.38 %). Interleukin-2 level was significantly elevated in mice inoculating with most parasite isolates, whereas interleukin-9 and interleukin-10 levels were not altered considerably experimentally in infected and treated mice compared with untreated control. Albendazole and praziquantel markedly reduced the number and size of secondary hydatid cysts compared to the untreated control group in Echinococcus granulosus-infected mice and stimulated the production of interleukin-2, which is helpful for parasite elimination. These results indicate that albendazole and praziquantel have protoscolicidal effects and the combination of albendazole and praziquantel has significant additive effects.

Prevalence and fertility Status of hydatid cysts in Sheep and Goats slaughtered in selected abattoirs in Adamawa State, Nigeria

Hydatidosis is one of the neglected zoonotic diseases of unrecognised importance that is caused by the dog tapeworm of thegenus Echinococcus. A study on the prevalence and fertility of hydatid cysts was conducted in sheep and goats slaughtered insome abattoirs in Adamawa State, Nigeria. A total of 1,603 animals comprising 760 sheep and 843 goats were examined forhydatid cysts by visual inspection, palpation and incision. A total of 31 (1.93%) of the study population harboured hydatidcysts, comprising 72 hydatid cysts in 13 (1.71%) sheep and 116 hydatid cysts in 18 (2.14%) goats. There was no significantdifference (p&gt;0.05) in prevalence of hydatid cyst infection among sheep and goats. Age –specific prevalence of hydatid cystswas higher in goats 18(2.1%) than Sheep 13(1.7%), goats and sheep that were &gt;3years old recorded highest prevalence(3.25%; 2.32%). There was no significant difference (p&gt;0.05) in prevalence of cysts in different age groups. Prevalence ofcysts was higher in female sheep (1.96%) and goats (2.98%) than male sheep (1.49%) and goats (1.66%), the difference wasnot statistically significant (p&gt;0.05). The number of hydatid cysts recovered in infected sheep was 72(25.0% fertile, 31.9%sterile and 43.0% calcified cysts) and 116 (47.4% fertile, 15.5% sterile and 25.9% calcified cysts) in goats. Highest number offertile cysts were recovered in lungs of sheep and goats (41.7%; 83.7%) than in their liver ( 16.7%; 37.8%). The differencewas not statistically significant (p&gt;0.05). The most commonly infected organ was liver (66.7%) in sheep and lungs (68.1%)in goats. In conclusion, fertility of cysts in sheep and goats may serve as a potential source of transmission of hydatidosis todogs and continuation of its lifecycle. Strict regulation of slaughtering process including proper disposal of infected offalwould minimize transmission of cysts from intermediate to definitive hosts.&#x0D; Keywords: Prevalence, Hydatid cysts, fertility, Sheep, Goat, Adamawa State

Cystic echinococcosis in slaughtered cattle at Addis Ababa Abattoir enterprise, Ethiopia

Cystic echinococcosis, caused by larval stages of the tapeworm Echinococcus granulosus, is responsible for hydatid cysts in the lungs, liver and other organs of intermediate hosts. A cross-sectional Abattoir study was conducted from October 2015 to May 2016 at Addis Ababa Abattoir enterprise, Ethiopia. For this purpose a total of 1209 cattle were examined using the classical meat inspection procedure for the presence of hydatid cysts. An overall prevalence of 21% (254/1209) of hydatid cyst was recorded in cattle slaughtered at Addis Ababa Abattoir enterprise. The prevalence of hydatid cyst was statistically significantly higher (P<0.05) in cattle with the age group of >6 years than those cattle with all other age categories. The study showed that lungs and livers were the most affected organs of cattle. Significantly (P < 0.05) higher overall number of hydatid cysts were observed in the lungs (63.9%; 1324/2071) than in the livers (35.4%; 734/2071). The study revealed the presence of an overall of 84.1% (333/396) sterile, 12.2% (48/396) calcified and 4.3% (17/396) fertile cysts. Of the total of 17 fertile hydatid cysts tested for viability, 17.6% (3/17) viable and 82.3% (14/17) nonviable cysts were observed. Overall, findings of the present study showed the widespread occurrence of hydatid cysts in internal organs of cattle that possibly incur significant economic losses through condemnation of edible organs rendering unfit for human consumption. Molecular studies on the role of intermediate host species, genotypes of E. granulosus, zoonotic impact and economic significance of echinococcosis are needed in different parts of Ethiopia.

Prevalence of hydatidosis and fertility of hydatid cysts in food animals in Northern Greece.

This study was conducted in order to determine the prevalence of hydatidosis due to Echinococcus granulosus sensu lato and the fertility rate of hydatid cysts in sheep, goats, cattle, and pigs slaughtered at the abattoir of Lagkada, in the Prefecture of Thessaloniki, Northern Greece. Lungs and livers from a total of 294 sheep (31.3% infected), 126 goats (8.7% infected), 372 cattle (4.8% infected), and 641 pigs (1.7% infected) were examined. The number of hydatid cysts found in infected sheep was 657 (58.3% fertile, 12.8% sterile, and 28.9% calcified cysts), in goats 54 (14.8% fertile, 38.9% sterile, and 46.3% calcified cysts), in cattle 108 (24.1% fertile, 50.0% sterile, and 25.9% calcified cysts) and in pigs 47 (10.6% fertile, 55.4% sterile, and 34.0% calcified cysts). Lung infection with hydatid cysts was higher (p<0.05) in sheep, goats, and cattle than in pigs (56.5%, 77.8% and 62.0% vs. 17.0%, respectively); while liver infection was lower (p<0.05) in sheep, goats, and cattle than in pigs (43.5%, 22.2% and 38.0% vs. 83.0%, respectively). Among the 4 species slaughtered for human consumption, sheep exhibited the highest frequency of hydatidosis and the highest proportion of fertile cysts.

Comparative prevalence of hepato-pulmonary hydatidosis among native and imported cattle in north of Sistan and Baluchestan: Iran

The objective of present study was comparison of hepato-pulmonary hydatidosis among imported and native cattle. The present study was performed during 2011-2012. 2,657 and 525 imported and native cattle were inspected in slaughter house of Zahedan and Zabol respectively. The present and number of hydatid cysts were evaluated. Also the fertility rate of cysts has been estimated. The prevalence of infection in imported and native cattle were 15.1 and 5.3% respectively and the statistical correlation was significant (P value <0.001). Also the fertility rate of hydatid cysts in the imported and native cattle were 16 and 18.2% respectively and the statistical correlation was not significant (P value >0.05). The results of present study shows significant differences in hydatidosis rate between native Iranian and imported cattle in Sistan and Baluchestan provinces of Iran. Slaughtering of imported livestock should be done in industrial centers to prevent potential increasing in canine echinococcosis and consequently human and farm animals hydatidosis.

Prevalence of cystic echinococcosis in pigs from Slovakia, with evaluation of size, fertility and number of hydatid cysts

Abstract The regional occurrence of cystic echinococcosis in slaughtered pig from Slovakia was studied in the period of 2000–2008, along with the quantitative parameters associated with the establishment of cysts (intensity of infection, fertility, size). From 103 pig livers collected from abattoirs in 35 sites Slovakia as suspected for Echinococcus infection, 63 were positive for cystic echinococcosis, whereas in 40 livers cysticercosis was diagnosed. Fertile cysts with echinococcal protoscoleces were recorded in 25.4 % of positive pigs, with the 8.9 % fertility rate and the intensity of infection reaching value of 5.9 cysts per liver. The average size of fertile cysts was more than three times larger than diameter of sterile cysts (diameters of 4.67 cm vs. 1.37 cm, respectively). No significant differences in relative amounts of fertile and sterile cysts were found among counties (P = 0.15). The annual prevalence of E. granulosus in pigs in 2000–2 008 has ranged from 0.02 % to 0.13 % (average rate 0.08 %), with the decreasing tendency over the period, especially after 2005. The most heavily affected areas with cystic echinococcosis over 2006–2008 were indicated in Prešov (eastern Slovakia, 0.68 % prevalence) and Komárno districts (southwestern Slovakia, 0.26 % prevalence). Obtained data are relevant in designing regional control strategies to suppress the occurrence of disease in livestock and risk for humans to be infected.

Povidone-iodine in experimental peritoneal hydatidosis

The effect of polyvinylpyrrolidone-iodine (PVP-I) on experimental peritoneal hydatidosis was evaluated in this randomized blind controlled study. Seventy-five white, 3-month-old rats were subjected to laparotomy. After the intraperitoneal inoculation of viable scolices, the rats were randomly divided into three groups. Their peritoneal cavities were irrigated with either 1 per cent PVP-I solution (the PVP-I group), hypertonic saline (the HS group) or Ringer's lactate (the control group). Each group was assigned a separate code number; observers blind to the meaning of the code numbers noted all findings during a period of 4 months, after which the rats were killed to allow assessment of the abdominal cavity. The results were decoded after the statistical analyses were completed. The incidence of peritoneal cysts was found to be lower (8.7 per cent) in the PVP-I group compared with the HS (50 per cent) and control (90.9 per cent) groups; the mean number of hydatid cysts per animal was also lower in the PVP-I group compared with the other two groups. We conclude that the scolicidal activity of PVP-I is significantly higher than that of hypertonic saline and that it can be employed as a prophylactic agent against peritoneal hydatidosis.

Ovine Echinococcus granulosus transmission dynamics in the province of Rio Negro, Argentina, 1980–1999

In this work, the impact of a hydatidosis control programme for dogs on the intermediate ovine host was specifically analysed to determine the levels of prevalence achieved and the dynamics of parasite transmission, as well as to evaluate the quality of diagnostic systems in slaughterhouses. A field study was conducted in four slaughterhouses (Valcheta, Los Menucos, Jacobacci, Bariloche) that process animals coming from all the departments within the work area. The control programme for dogs entailed treating dogs with 5 mg/kg praziquantel at 2-month intervals for 20 years. Sample size was determined with a 10% error margin and a 95% significance level. Harvested viscera (liver, lungs, kidneys) were preserved in 5% formaldehyde and sent to the laboratory for diagnostic confirmation of both positive and negative specimens. The 61% initial prevalence dropped to 18.3% at the end of the 10-year period, observed differences proving significant (Chi-square=15.454, P=0.00). There were statistically significant increases in infection prevalence with age (Pearson’s Chi-square=133.61, P=0.00). Overall, 37.2% of hydatidosis cases diagnosed in slaughterhouses were considered non-hydatid by histological study. On the other hand, 1.1% of those diagnosed as healthy were found to be infected with hydatidosis. The number of hydatid cysts per animal increased with age: 0.04 in lambs and 1.22 in adults (linear regression equation, −0.0539+0.0127×age), whereas the average for the whole period was 3.7% in lambs and 20.5% in adults. Viability studies indicated that 63.8% of parasitised animals had viable cysts, out of which 53.3% were fertile. Diagnosis of infection in sheep made by means of an adjusted statistical design and with histological confirmation of the presumptive diagnosis made in slaughterhouses demonstrated the flaws of the official systems for epidemic surveillance of hydatidosis. However, there was no overall significant difference in slaughterhouse and laboratory data. Ro was 0.53 and could imply a decrease in reproductive capacity of the parasite and steady state extinction.

Epidemiology of hydatid disease in Sardinia: a study of fertility of cysts in sheep

Hydatidosis, caused by Echinococcus granulosus, is a cyclozoonotic disease of economic significance in Sardinia. The life-cycle involves stray and sheep dogs as definitive hosts and sheep, pigs, goats and cattle as intermediate hosts. The most important intermediate host is sheep, due to home slaughtering with ready access of the viscera to dogs. This survey was undertaken in 1987 to ascertain the epidemiological significance of sheep in maintaining the life-cycle. A total of 700 (91.3%) of 767 sheep harboured hydatid cysts. The frequency distribution of the number of hydatid cysts was over-dispersed. Of 497 infected sheep, 7.6% had fertile cysts, 75.7% sterile cysts and 16.7% fertile + sterile cysts.

Prevalence of hydatid in buffaloes in India and report of a severe liver infection.

Of 3200 buffaloes examined at Bareilly abattoir 915 (28.6%) were positive for hydatidosis. The hydatid infected organs were lungs (60%), liver (32%), spleen (4%), kidneys (2%), heart (0.9%) and brain (0.1%). The number of hydatid cysts encountered in lungs of an individual buffalo was 1-9 and 1-7 in liver. One buffalo liver showed severe hepatomegaly, cirrhosis and had an exceptionally high number of hydatid cysts. Infectivity studies in pups showed 16-29 per cent worm establishment with the presence of shelled eggs within the uterus of the parasite on day 45 post-infection.