Number Of Antibody-producing Cells Research Articles

The Number of Antibody-Producing Cells in the Parathymic Lymph Nodes and the Spleen Following Intraperitoneal Injection of Antigen

Summary Following i.p. injection of antigen in the rat, the parathymic lymph nodes are α major, if not the major, site of antibody-producing cells. Though they contain only one-tenth the number of nucleated cells, these nodes have essentially as many antibody-forming cells as does the entire spleen. Technical limitations that occur when the spleen is employed for such studies are also minimized or absent when the PLN are used to determine the number of PFC by the modified Cunningham technique. Thus, the parathymic lymph nodes should receive increased attention in investigating the cellular kinetics of the immune response.

Cytokinetics of IGG vs IGM PFU response in the lungs of C3H mice.

The hemolytic plaque assay procedure developed by Jerne 1 and by Ingraham and Bussard 2 has been used to evaluate the relative number of antibody-producing cells in the population. The number of plaque-forming cells detected during the primary and secondary responses is related to the levels of antibody titers attained in the circulation which in turn determines the immune status of the animal. The contribution of antibodies to the circulation by antibody-forming cells residing in extralymphoidal tissues is not known. The possibility that significant amounts may come from this source cannot be ignored.If, in the course of preliminary stimulation, immunocompetent cells seed or are stimulated in situ in nonlymphoid organs, activation of these cells and their progeny by secondary stimulation will indicate their whereabouts.These studies were initiated to determine the presence of antibody-forming cells in the lung of hyperimmunized mice.Materials and Methods. C3H strain mice were injected with 0.1 ml of a 50...

Influence of human rheumatoid factor on numbers of antibody-producing cells.

AbstractHuman rheumatoid factor administered to mice 24 hours after intraperitoneal injection of human IgC as antigen causes a 68 per cent reduction in the numbers of spleen cells capable of forming rosettes with IgG‐coated sheep erythrocytes. Normal human macroglobulin does not cause such an inhibition of antibody‐producing cells. The inhibitory effect of rheumatoid macroglobulin appears immunologically specific.