Melanin concentrating hormone (MCH) is a highly conserved cyclic peptide present in vertebrates. In this study, we describe the organization of MCH-immunoreactive (MCH-ir) cells and fibres in different regions of the brain in the cichlid fish Oreochromis mossambicus. Only MCH-ir fibres were observed in dorsal and ventral subdivisions of the telencephalon, the preoptic area and magnocellular and parvocellular divisions of the nucleus preopticus, and in hypothalamic areas such as the suprachiasmatic nucleus and tuberal area. Distinctly labelled MCH-ir perikarya were observed in the paraventricular organ, lateral and medial subdivisions of the nucleus lateralis tuberis, nucleus recessus lateralis and in the nucleus posterior tuberis. The pituitary gland showed MCH-ir fibres in the proximal pars distalis, neurohypophyseal ramifications and in pars intermedia where the dark accumulations of MCH-ir content corresponded to enlarged axon terminals. In the diencephalon, MCH-ir fibres were also labelled in the pretectal area, thalamic nuclei and preglomerular complex. In the midbrain tegmentum, a cluster of MCH-ir neurons was detected in the dorsal tegmental nucleus, whereas MCH-ir fibres were distributed in the torus semicircularis and optic tectum. In the rhombencephalon, MCH-ir fibres were located in the nucleus lateralis valvulae, cerebellum and secondary gustatory nucleus. Overall, the widespread distribution of MCH-ir cells and fibres in the brain suggests diverse roles for MCH such as regulation of sensorimotor and neuroendocrine functions in the tilapia.