You have accessJournal of UrologyBladder Cancer: Basic Research I1 Apr 2010792 EVALUATION OF GENE EXPRESSION BY NUCLEOSTEMIN AND FAMILY MEMBERS IN BLADDER CANCER Hiroaki Yasumoto, Miho Hiraki, Naoko Arichi, Yozo Mitsui, Takeo Hiraoka, Koji Wake, Masahiro Sumura, Satoshi Honda, Robert Tsai, Hiroaki Shiina, and Mikio Igawa Hiroaki YasumotoHiroaki Yasumoto Izumo, Japan More articles by this author , Miho HirakiMiho Hiraki Izumo, Japan More articles by this author , Naoko ArichiNaoko Arichi Izumo, Japan More articles by this author , Yozo MitsuiYozo Mitsui Izumo, Japan More articles by this author , Takeo HiraokaTakeo Hiraoka Izumo, Japan More articles by this author , Koji WakeKoji Wake Izumo, Japan More articles by this author , Masahiro SumuraMasahiro Sumura Izumo, Japan More articles by this author , Satoshi HondaSatoshi Honda Izumo, Japan More articles by this author , Robert TsaiRobert Tsai Houston, TX More articles by this author , Hiroaki ShiinaHiroaki Shiina Izumo, Japan More articles by this author , and Mikio IgawaMikio Igawa Izumo, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1441AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Nucleostemin (NS) is a positive regulator of cell proliferation and highly expressed in a variety of stem cells, tumors, and tumor cell lines. NS, guanine nucleotide binding protein-like 3 (GNL3L), and GNL2 constitute the nucleostemin family, however, differences in their expression and physiologic functions in bladder cancer (BCa) remain unclear. Herein, we evaluated NS, GNL3L, and GNL2 expressions in BCa, and their effects on proliferation in vitro. METHODS BCa and non-BCa tissues (n=94) were collected from consecutive patients undergoing TUR or a cystectomy. Total RNA was isolated from the tissues and subjected to real time RT-PCR for NS, GNL3L, GNL2, and GAPDH using specific TaqMAN probes. We also established stable NS transfectants from T24 cells for gain of function studies, with which cellular proliferation was examined using MTT assays. RESULTS The mean NS, GNL3L, and GNL2 mRNA levels were 1.6-, 1.6- and 14.5-fold greater in BCa tissues (4.64±1.03, 0.36±0.09, and 7.92±1.4, respectively) as compared to non-BCa tissues (2.86±0.34, 0.22±0.03, and 0.054±1.4, respectively; P=0.014, =0.0016, and <0.0001, respectively; Wilcoxon signed-rank test) (Fig. 1). In vitro, cellular proliferation assays demonstrated a significant increase in growth of NS-transfected T24 cells as compared with the control and wild-type T24 cells (P=0.0001, repeated ANOVA) (Fig. 2). CONCLUSIONS This is the first known report to show that nucleostemin and family gene mRNA expressions are up-regulated in BCa, especially GNL2. Our results suggest that NS family genes are tumor promoting factors in BCa. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e309-e310 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Hiroaki Yasumoto Izumo, Japan More articles by this author Miho Hiraki Izumo, Japan More articles by this author Naoko Arichi Izumo, Japan More articles by this author Yozo Mitsui Izumo, Japan More articles by this author Takeo Hiraoka Izumo, Japan More articles by this author Koji Wake Izumo, Japan More articles by this author Masahiro Sumura Izumo, Japan More articles by this author Satoshi Honda Izumo, Japan More articles by this author Robert Tsai Houston, TX More articles by this author Hiroaki Shiina Izumo, Japan More articles by this author Mikio Igawa Izumo, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...