The Deep Impact mission succeeded in excavating inner materials from the nucleus of Comet 9P/Tempel 1 on 2005 July 04 (at 05:52 UT). Comet 9P/Tempel 1 is one of Jupiter family short period comets, which might originate in the Kuiper belt region in the solar nebula. In order to characterize the comet and to support the mission from the ground-based observatory, optical high-dispersion spectroscopic observations were carried out with the echelle spectrograph (UVES) mounted on the 8-m telescope VLT (UT2) before and after the Deep Impact event. Ortho-to- para abundance ratios (OPRs) of cometary ammonia were determined from the NH 2 emission spectra. The OPRs of ammonia on July 3.996 UT and 4.997 UT were derived to be 1.28 ± 0.07 (nuclear spin temperature: T spin = 24 ± 2 K ) and 1.26 ± 0.08 ( T spin = 25 ± 2 K ), respectively. There is no significant change between before and after the impact. Actually, most materials ejected from the impact site could have moved away from the nucleus on July 4.997 UT, about 17 h after the impact. However, a small fraction of the ejected materials might remain in the slit of UVES instrument at that time because an excess of about 20% in the NH 2 emission flux is observed above the normal activity level was found [Manfroid, J., Hutsemékers, D., Jehin, E., Cochran, A.L., Arpigny, C., Jackson, W.M., Meech, K.J., Schulz, R., Zucconi, J.-M., 2007. Icarus. This issue]. If the excess of NH 2 on July 04.997 UT was produced from icy materials excavated by the Deep Impact, then an upper-limit of the ammonia OPR would be 1.75 ( T spin > 17 K ) for those materials. On the other hand, the OPR of ammonia produced from the quiescent sources was similar to that of the Oort cloud comets observed so far. This fact may imply that physical conditions where cometary ices formed were similar between Comet 9P/Tempel 1 and the Oort cloud comets.