The global concerns about safety in the digital technology of the main control room (MCR) are growing as domestic and foreign nuclear power plants are developed with computerized control facilities and human–system interfaces. In a narrow space, the digital technology contributes to a control room environment, which can facilitate the acquisition of all the information needed for operation. Thus, although an individual performance of the advanced MCR can be further improved; there is a limit in expecting an improvement in team performance. The team performance depends on organic coherence as a whole team rather than on the knowledge and skill of an individual operator. Moreover, a good team performance improves communication between and within teams in an efficient manner, and then it can be conducive to addressing unsafe conditions. Respecting this, it is important and necessary to develop methodological technology for the evaluation of operators’ teamwork or collaboration, thus enhancing operational performance in nuclear power plant at the MCR. The objectives of this research are twofold: to develop a systematic methodology for evaluation of the team performance of MCR operators in consideration of advanced MCR characteristics, and to validate that the methodology is adaptable to the advanced MCR of nuclear power plants. In order to achieve these two objectives, first, team performance factors were extracted through literature reviews and methodological study concerning team performance theories. Second, the team performance factors were identified and behavior markers were deducted based on the factors. Lastly, an estimation model for the evaluation of team performance was developed, and the model thereon was validated. The estimation model consists of an evaluation of task characteristics, identification of team performance factors, evaluation of team performance, calculation of team performance, and analysis of team performance. The detailed contents and validations of the model are also presented in this paper.