Nuclear Characteristics Research Articles

The Accurate Interpretation and Clinical Significance of Morphological Features of Fine Needle Aspiration Cells in Papillary Thyroid Carcinoma.

Papillary thyroid carcinoma (PTC) is the most common malignant neoplasm of the thyroid gland; fine needle aspiration cytology is the most basic and reliable diagnostic method before PTC operation. However, it is not clear which cell morphological changes can be used as a reliable standard for the diagnosis of PTC. A retrospective analysis was performed on 337 patients with PTC confirmed by postoperative histology. An additional 197 randomly selected patients with benign thyroid lesions were included in the study and used as a control group. True papillary arrangements, swirl arrangements, and escape arrangements had high specificity, all of which were 100%, but only swirl arrangements had ideal sensitivity (77.61%). The nuclear volume characteristics had a high sensitivity of more than 90%, but the specificities of both nuclear crowding and nuclear overlap were too low, only 16.34% and 23.35%. The sensitivities of five nuclear structural characteristics were more than 90%, but only the specificity of intranuclear cytoplasmic pseudoinclusions (INCIs) reached 100%, nuclear contour irregularity and pale nuclei with powdery chromatin also had ideal interpretation value except for grooves and marginally placed micronucleoli. Although the sensitivity of psammoma bodies (PBs) was low, the specificity was 100%. In terms of preparation methods, the method of liquid-based preparation (LBP) is obviously better than that of conventional smears. The diagnostic efficiency by the combined detection method of parallel tests showed that without reducing the specificity, the sensitivity increased with the increase of the number of morphological characteristics and finally reached 98.81%. The INCIs and swirl arrangements are the most common and important indicators for the diagnosis of PTC, whereas papillary-like arrangements, the crowding and overlap of nuclear, grooves, marginally placed micronucleoli, and multinucleated giant cells are of little significance for the diagnosis of PTC.

Effect of blanching and ultrasound pretreatment on moisture migration, uniformity, and quality attributes of dried cantaloupe.

To overcome problems of browning and crusting during the pretreatment process and provide theoretical guidance for cantaloupe convection drying at 80°C, the effects of blanching (BL) and ultrasonic (US) treatments were examined. The effects of various BL (5, 10, and 15 s) and US (10, 20, 30, and 40 min) durations on convection drying were tested. The moisture ratio, drying rate, moisture effective diffusivity, color, browning, nuclear magnetic resonance characteristics, and texture were assessed. Compared with the control group, the maximal decreases in the drying time of BL and US pretreatment groups were 40% and 33.3%, respectively. BL and US pretreatments significantly increased the effective diffusion coefficient and shortened the drying time because of the destruction of the cell structure. Low-field nuclear magnetic resonance analysis showed that free water is mainly lost during the initial drying stage, while solidified water is mainly lost during middle and late stages. According to the results of magnetic resonance imaging, the moisture distribution shows that cavitation from US acts on internal tissue, while BL disrupts the structure of external tissue. Texture data define the area enclosed by SC-D as uniform. After BL and US pretreatment, the hardness of dried cantaloupe decreased and the uniformity increased significantly. The best pretreatment process for cantaloupe at 80°C was 10 min of US. These findings provide a reference for testing in the industrial production of dried cantaloupe and are deeply relevant for practice.

Investigation of nuclear reactor core thermal-hydraulic characteristics after partial loss of flow accident

In normal operation conditions of nuclear power plants, the distribution of primary coolant between fuel channels would be considered almost uniform. When different number of Reactor Circulation Pumps (RCPs) are switched off, known as an abnormal condition, this uniform distribution is disturbed and different conditions occur for each channel depending on its position in the core. In this research, the normal and abnormal condition (with one or two tripped RCPs) for a VVER-1000/446 is investigated. For evaluation of the core neutronic calculations and thermal power distribution, USNRC’s PARCS system code is employed. Then a thermal-hydraulics module was developed for performing the T/H calculation of the core zone. The input velocity of each channel in abnormal condition was calculated based on developed CFD model in downcomer and lower plenum of Reactor Pressure Vessel (RPV) by ANSYS-CFX. The results show that, in normal operation, the hot channel is related to the central fuel assembly of the reactor core with the highest relative power equal to 1.29 and total power of 23.74 MW. In this case, the minimum inlet velocity, the maximum coolant outlet velocity, and the maximum fuel temperature are 5.6 (m/s), 330.96 (°C), and 1345.8 (°C), respectively. In the cases of operation with one and two tripped RCPs, the hot channel is related to the fuel assemblies with the lowest inlet velocities. The lowest velocities are 0.32 m/s, 0.24 m/s, and 0.22 m/s respectively for the condition with one tripped pump, two tripped pumps placed oppositely, and two tripped pumps placed contiguously. The hot channel numbers in these cases are 158, 102, and 90, respectively. In these channel, the condition of outlet flow would be superheated, but the fuel temperature (1006.3, 1050.9, and 987.9) do not reach the maximum allowable margin. The study confirms the necessity of the coolant distribution consideration in OLCs as well as events that may disturb the symmetry of the coolant flow. It also showed that the lateral fuel assemblies are more at risk in this situation because of a significant reduction in coolant flow. Likewise, the investigations proved the safe continuation of the operation in PLOFA conditions with the preventive algorithm of the emergency protection system and without any need for immediate mitigation actions or operator intervention.

ОПРЕДЕЛЕНИЕ УДЕЛЬНОЙ АКТИВНОСТИ 177Lu И СОПУТСТВУЮЩИХ РАДИОНУКЛИДНЫХ ПРИМЕСЕЙ

Radioactive isotopes of rare earth elements (REE) are increasingly used in nuclear medicine for diagnostic and therapeutic purposes as they have effective nuclear physical properties. Seventeen elements of the secondary subgroup of group III of the periodic table of chemical elements of D.I. Mendeleev with atomic numbers 21, 39, 57, 58-71: scandium, yttrium, lanthanum and 14 lanthanides make up rare earth elements. Every year more and more attention is paid to the radioactive isotope of lutetium 177Lu, the most promising for use in therapeutic agents of a new generation, including for cell therapy of oncological diseases. The advantages of 177Lu are determined by its nuclear characteristics: the optimal depth of penetration into human tissues during radiotherapy of small tumors and low radiation load on healthy organs are provided due to the maximum energy of beta radiation of 496 keV, the presence of low-energy gamma radiation of 113 keV allows monitoring the treatment process, the half-life of 6.71 days makes it possible to transport drugs from 177Lu to significant distances.

Jets in FR0 radio galaxies

Context. The local radio-loud AGN population is dominated by compact sources named FR0s. These sources show features, for example the host type, the mass of the supermassive black hole (SMBH), and the multi-band nuclear characteristics, that are similar to those of FRI radio galaxies. However, in the radio band, while FR0 and FRI share the same nuclear properties, the kiloparsec-scale diffuse component dominant in FRI is missing in FR0s. Previous very-long-baseline interferometry (VLBI) observations of a small sample of FR0s show a complex structure, mostly symmetric (two-sided jets) with respect to the central core. Aims. With this project we would like to study the parsec-scale structure in FR0s in comparison with that of FRI sources. Jets in FRI are relativistic on the parsec scale and decrease their velocity becoming subrelativistic on the kiloparsec scale. We would like to test whether this result also applies to the jets in FR0s or, alternatively, whether they are subrelativistic on the parsec scale. This might be the reason why they are unable to grow, because of instabilities, related to a low jet bulk velocity. Methods. To this end we observed 18 FR0 galaxies with the VLBA at 1.5 and 5 GHz and/or with the EVN at 1.7 GHz and produced detailed images at milliarcsec resolution of their nuclear emission to study the jet and core structure. Results. All sources have been detected but one. Four sources are unresolved, even in these high-resolution images; jets have been detected in all other sources. We derived the distribution of the jet-to-counter-jet ratio of FR0s and found that it is significantly different from that of FRIs, suggesting different jet bulk speed velocities. Conclusions. Combining the present data with published data of FR0 with VLBI observations, we derive that the radio structure of FR0 galaxies shows strong evidence that parsec-scale jets in FR0 sources are mildly relativistic with a bulk velocity on the order of 0.5c or less. A jet structure with a thin inner relativistic spine surrounded by a low-velocity sheath could be in agreement with the SMBH and jet launch region properties.

Development of a Passive Reactor Shutdown Device to Prevent Core Disruptive Accidents in Fast Reactors: A Preliminary Study

AbstractFollowing the Fukushima Nuclear Power Plant accident in 2011, it has become increasingly important for reactor safety designs to consider measures that can prevent the occurrence of severe accidents. This report proposes a novel subassembly-type passive reactor shutdown device that expands the diversity and robustness of core disruptive accident (CDA) prevention strategies for sodium-cooled fast reactors. The developed device contains pins with a fuel material that is in the solid state during normal operation but melts into a liquid when the temperature exceeds a certain value (i.e., during a potential accident). When an unprotected loss of flow (ULOF) or unprotected transient overpower (UTOP) accident occurs, the device can passively provide significant negative reactivity by rapidly transferring liquefied device fuel into the lower plenum region of the pins via gravitation alone. The reactors containing some of the proposed devices in place of original fuel subassemblies become subcritical before the driver fuels are damaged, even if ULOF or UTOP transient events occur. The present study evaluates candidate materials for device fuels (e.g., metallic alloy, chloride), optimal device pin structures for liquefied fuel relocation, and nuclear and thermal-hydraulic characteristics of the device-loaded core under accident conditions to demonstrate the engineering applicability of the proposed device. This report discusses preliminary results regarding the nuclear requirements for inducing negative reactivity to achieve reactor shutdown under the expected device conditions during an accident.

Synthesis and Ready to Use Kit Formulation of EDTMP for the Preparation of 177Lu-EDTMP as a Bone Palliation Radiopharmaceutical.

With its suitable nuclear decay characteristics and large-scale production feasibility with adequate specific activity, 177Lu is regarded as an excellent radionuclide for developing bone pain palliation agent. Ethylenediamine-tetramethylene phosphonic acid (EDTMP) is a preferred carrier molecule for radiolanthanides, such as 177Lu. The present paper describes the synthesis of EDTMP and the development of a ready-to-use kit for the preparation of 177Lu-EDTMP and its quality control in accordance with the quality and safety criteria required for medicinal use. EDTMP was synthesized by a modified Mannich-type reaction, and the structure was characterized using NMR and IR spectroscopy. Optimization of radiolabeling conditions was done with two different salt forms of EDTMP. The labeling yield was checked by paper chromatography with radiation detection. Kit was developed as a lyophilized mixture of EDTMP and sodium bicarbonate in a maximum volume of 5 mL. Labeling efficiency, radionuclidic purity, radiochemical purity, sterility, and pyrogenicity analysis were performed as the quality control of the labeled kit. The analytical data for the structure determination and purity of the synthesized ligand were in agreement with authentic commercial samples used in radiopharmacy.177Lu-EDTMP complex was prepared using synthesized EDTMP ligand under optimized labeling conditions with high labelling yield (>99%). The radiolabeling yields of the EDTMP kit at room temperature after 30 min and 48 hours were 99.46% and 99.00%. The developed EDTMP kit enables an instant one-step preparation of the radiopharmaceutical of high radiochemical purity (>99%) and has a sufficiently long shelf life. This enables the routine production of the 177Lu-EDTMP in nuclear medicine clinics without requiring experienced staff.

Fractal Dimension and Nuclear Magnetic Resonance Characteristics of Surfactants for Coal Gas Desorption

In this paper, the fractal dimension of coal surfaces was calculated using the fractal theory, and the influence of different surfactants on the fractal dimension of coal surface was studied. Six kinds of sustainable and traditional surfactants used in coal gas desorption processes were compared and analyzed. We used mineral gas fertilizer coal from Huainan Liuzhuang, China, as the research object and studied sodium dodecyl benzene sulfonate (SDBS), cohol diethanolamide (CDEA), nonylphenol polyoxyethylene ether (NP-10), tea saponin, sucrose ester and rhamnolipid surfactants used to modify coal. The methane adsorption capacity of coal before and after surfactant modification was measured by low field nuclear magnetic resonance methane adsorption, and Langmuir volume and Langmuir pressure were obtained according to the Langmuir fitting equation. The results showed that from the perspective of fractal dimensions, the best surfactant in the context of sustainability (in order) is rhamnolipid, CDEA, tea saponin, sucrose ester. According to the two important parameters VL and PL in the Langmuir equation, the coal sample modified by sucrose ester had the strongest gas adsorption capacity. According to the numerical analysis of the surface fractal dimension DS of the coal modified by surfactants, the soluble organic matter in the raw coal samples dissolved, and the micropore morphology evolved to become mesoporous or macroporous, which is more favorable for desorption. The coal samples modified by rhamnolipid and SDBS had the strongest gas desorption ability.

Nuclear magnetic resonance response characteristics and quantitative evaluation method of fluid saturation of lacustrine shale oil

The quantitative evaluation of fluid saturation is important for formation evaluation of shale oil. However, there is currently no effective method to identify the fluid occurrence state and quantitative evaluate the fluid saturation of lacustrine shale oil because of the complexity of diagenetic minerals and pore types. In this paper, a method is proposed for the quantitative evaluation of fluid saturation based on nuclear magnetic resonance (NMR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) measurements for shale samples of Fengcheng Formation, Mahu sag in Junggar Basin, China. These studies revealed that the shale oil rocks mainly contain quartz, feldspar, dolomite, calcite and clay minerals, both develop organic and inorganic pores. The fluids mainly occur in the form of bitumen, clay bound water, bound water, bound oil and movable oil in this study area. Based on the findings from these experiments, a mixed rock index (MI) and mud index (SI) were proposed to divide the shale oil formations into three types, including sand shale, dolomitic shale and mudstone. A T1−T2 2D NMR fluid occurrence state characterization map was established to identify the different fluids by the MI, SI and NMR characteristics. Furthermore, a method was proposed to quantitatively calculate the coefficient distribution of bound and movable fluids for shale oil in different formations. Finally, the proposed method was successfully applied into the lacustrine shale oil in the Fengcheng Formation to identify fluid occurrence state and evaluate fluid saturation quantitatively.

CorTAF: A nuclear reactor core three-dimensional thermal-hydraulic characteristics analysis code based on OpenFOAM

In this paper, a thermal hydraulic analysis method based on open source CFD platform OpenFOAM was proposed. The models of coolant flow and heat transfer, fuel rod heat conduction and coupled heat transfer were established according to the bundle structure characteristics of PWR core, and then a nuclear reactor three-dimensional thermal–hydraulic characteristics analysis code CorTAF was developed based on finite volume method with the spatialresolution of subchannel level. The international benchmarks, including GE3 × 3, Weiss and PNL2 × 6 fuel assembly flow and heat transfer experiments, were selected to carry out the validation. The calculation results were basically consistent with the experimental data, illustrating that CorTAF is suitable for predicting the flow and heat transfer characteristics of coolant in the rod bundle fuel assembly. The thermal–hydraulic characteristics of fuel assembly and PWR core under full power operation and blockage accident conditions were simulated using CorTAF. The steady-state and transient spatial distribution of physical quantities in subchannel-scale including coolant and fuel rod temperature were obtained, and the influence of blockage condition on flow and heat transfer characteristics was analyzed. This work has reference significance for the development of core thermal hydraulic analysis tools for PWR.

Transportin 1 is a major nuclear import receptor of the nitric oxide synthase interacting protein

The nitric oxide synthase interacting protein (NOSIP), an E3-ubiquitin ligase, is involved in various processes like neuronal development, craniofacial development, granulopoiesis, mitogenic signaling, apoptosis, and cell proliferation. The best-characterized function of NOSIP is the regulation of endothelial nitric oxide synthase activity by translocating the membrane-bound enzyme to the cytoskeleton, specifically in the G2 phase of the cell cycle. For this, NOSIP itself has to be translocated from its prominent localization, the nucleus, to the cytoplasm. Nuclear import of NOSIP was suggested to be mediated by the canonical transport receptors importin α/β. Recently, we found NOSIP in a proteomic screen as a potential importin 13 cargo. Here, we describe the nuclear shuttling characteristics of NOSIP in living cells and invitro and show that it does not interact directly with importin α. Instead, it formed stable complexes with several importins (-β,-7,-β/7,-13, and transportin 1) and was also imported into the nucleus in digitonin-permeabilized cells by these factors. In living HeLa cells, transportin 1 seems to be the major nuclear import receptor for NOSIP. A detailed analysis of the NOSIP-transportin 1 interaction revealed a high affinity and an unusual binding mode, involving the N-terminal half of transportin 1. In contrast to nuclear import, nuclear export of NOSIP seems to occur mostly by passive diffusion. Thus, our results uncover additional layers in the larger process of endothelial nitric oxide synthase regulation.

Noninvasive Follicular Thyroid Neoplasm With Papillary-Like Nuclear Features: What a Surgeon Should Know.

The inclusion of the less aggressive follicular form of papillary thyroid cancer (PTC) is associated with an increase in the incidence of the condition, with the follicular variant of PTC being the most common of all variants. The majority of individuals with the encapsulated follicular variant of papillary thyroid carcinoma (EFVPTC) are treated as though they have classic thyroid cancer, despite the availability of mounting evidence to contradict the aforementioned. According to numerous research, a certain type of noninvasive-EFVPTC (NI-EFVPTC) demonstrated poor histopathologic diagnostic reproducibility and has received aggressive treatment similar to that of a classical thyroid neoplasm. Therefore, to replace the term NI-EFVPC, a new nomenclature for these tumors, called "noninvasive follicular thyroid neoplasm with papillary-like nuclear characteristics" (NIFTP), was introduced in the year 2016. The present paper explores this recently introduced terminology, clinical, histologic, and molecular features, and diagnostic criteria.

Development of a robust nuclear data adjustment method to outliers

We developed a new nuclear data adjustment method for experimental data containing outliers. This method mitigates the effect of outliers by applying M-estimation, a type of robust estimation, to the conventional nuclear data adjustment method using sensitivity coefficients. Based on the M-estimation, we derived a weighted nuclear data adjustment formula and developed a weight calculation method. The weighted nuclear data adjustment formula was derived by weighting the function to take the extremum of the conventional nuclear data adjustment. The weighting of each nuclear characteristic is calculated from the difference between the measured and calculated values of the nuclear characteristic. This weight calculation method can evaluate the validity of each nuclear characteristic by considering correlations between nuclear characteristics using singular value decomposition. The proposed method and the conventional method were compared and verified by twin experiments. In the twin experiments, the nuclear data were adjusted using experimental data that intentionally included outliers. As a result of twin experiments, it was confirmed that the nuclear data were adjusted robustly and appropriately even with the experimental data containing outliers.

Indiscriminate Nodule Sampling in Goitre: A Series of 5 Cases of Thyroid Microcarcinoma

Papillary Thyroid Carcinoma (PTC), which is a form of thyroid cancer, originates from follicular epithelial cells and can be identified histopathologically by distinct nuclear characteristics and architecture. It is the most common thyroid neoplasm and is typically found in young females, with a good prognosis. Within the subset of PTC, exists incidentally detected lesions called papillary microcarcinomas (IPMC), having a size ≤1 cm which are usually clinically not suspected, radiologically undetected, and often missed in gross pathological examinations. The present case series highlights five cases (2 males and 3 females) of papillary microcarcinomas that were detected over the course of one year. The patients’ ages ranged from 31 to 60 years, with a mean age of 48.8±10.1 years. The male-to-female ratio was 1:1.5. These patients presented with complaints of neck swelling and were initially diagnosed with benign nodular goitre in 47 cases based on clinical and radiological evaluations. The detection rate of papillary microcarcinomas was 10.6%, with one case showing bilaterality (20%). The authors of the present case series adopted a novel approach of indiscriminate nodule sampling of thyroidectomy specimens to observe changes in detection rates. This approach facilitated a higher yield of papillary microcarcinomas. The authors also considered the dilemma of surgical measures and prognostic outcomes in such cases.

Discussion on variety of cross-section candidate obtained by cross-section adjustment method

The cross-section adjustment method gives only one best-estimated cross-section set based on Bayesian theory. In this study, cross-section sets other than the best-estimated set were discussed. The cross-section sets were obtained by the random sampling method using a covariance matrix adjusted by the cross-section adjustment method. 1,000,000 cross-section sets were obtained by the random sampling method. From these cross-section sets, significant cross-section sets other than the best-estimated set were considered. 593,340 cross-section sets that accurately evaluate nuclear characteristics were obtained. The result of the cross-section adjustment method shows that the change due to U235 χ is the largest positive. However, keff is not changed to the positive side by U235 χ in 179,994 of the 593,340 cross-section sets obtained by the random sampling method. Although the best-estimated cross-section set is obtained by the cross-section adjustment method, the result suggests there are various candidates for the adjusted cross-section set.

Cell and nuclear osmotic characteristics of adherent HepG2 cells detected by using confocal microscopy and 3D reconstruction: Implications for cryopreservation

Cell and nuclear osmotic characteristics of adherent HepG2 cells detected by using confocal microscopy and 3D reconstruction: Implications for cryopreservation

Alteration in the optical, structural, and nuclear radiation attenuation characteristics of polyvinyl alcohol/lead oxide (PVA/PbO) films

Alteration in the optical, structural, and nuclear radiation attenuation characteristics of polyvinyl alcohol/lead oxide (PVA/PbO) films

A transition phase in late mouse oogenesis impacts DNA methylation of the early embryo

A well-orchestrated program of oocyte growth and differentiation results in a developmentally competent oocyte. In late oogenesis, germinal vesicle oocytes (GVOs) undergo chromatin remodeling accompanied by transcriptional silencing from an NSN (non-surrounded nucleolus) to an SN (surrounded nucleolus) chromatin state. By analyzing different cytoplasmic and nuclear characteristics, our results indicate that murine NSN-GVOs transition via an intermediate stage into SN-GVOs in vivo. Interestingly, this transition can also be observed ex vivo, including most characteristics seen in vivo, which allows to analyze this transition process in more detail. The nuclear rearrangements during the transition are accompanied by changes in DNA methylation and Tet enzyme-catalyzed DNA modifications. Early parthenogenetic embryos, derived from NSN-GVOs, show lower DNA methylation levels than SN-derived embryos. Together, our data suggest that a successful NSN-SN transition in oogenesis including proper DNA methylation remodeling is important for the establishment of a developmentally competent oocyte for the beginning of life.

Serial changes of myocardial perfusion imaging in takotsubo and reverse takotsubo cardiomyopathy

BackgroundTakotsubo cardiomyopathy (TTC) shows reversible hypokinesis in the left ventricular (LV) apical-half segment and hyperkinesis in the LV basal-half segment. However, the precise pathophysiological mechanism of TTC is unclear. Therefore, this study sought to clarify the nuclear characteristics, degree of myocardial damage, and serial change of TTC and rTTC using myocardial perfusion imaging. MethodsWe performed myocardial perfusion scintigraphy in 28 patients (TTC: 20, rTTC: 8) using Tc-99m sestamibi and assessed minimum percentage uptake (min-%-uptake), extent score (ES) and summed rest score (SRS) at acute and chronic phases. ResultsMin-%-uptake improved from the acute to the chronic phase (TTC: 54 [48-59]% vs 87 [81-90]%, P < 0.01; rTTC: 60 [55-64]% vs 77 [71-79]%, P < 0.01), as did the ES (TTC: 32 [26-41]% vs 0.0 [0.0-6.0]%, P < 0.01; rTTC: 16 [12-34]% vs 0.0 [0.0-0.0]%, P = 0.02) and SRS (TTC: 4.5 [3.9-5.3] vs 0.0 [0.0-0.2], P < 0.01; rTTC: 3.6 [3.3-3.8] vs 0.0 [0.0-0.0], P = 0.01). ConclusionTc-99m sestamibi uptake was reduced in hypokinetic regions in the acute phase and improved in the chronic phase. TTC and rTTC may involve a reversible disorder of the myocardial cell membrane, mitochondria, and microcirculation.

H4picoopa─Robust Chelate for 225Ac/111In Theranostics.

The nuclear decay characteristics of 225Ac (Eα = 5-8 MeV, linear energy transfer (LET) = ∼100 keV/μm, t1/2 = 9.92 days) are well recognized as advantageous for the treatment of primary and metastatic tumors; however, suitable chelation systems are required, which can accommodate this radiometal. Since 225Ac does not possess any suitable low-energy, high abundance γ-ray emissions for nuclear imaging, there is a clear need for the development of other companion radionuclides with similar coordination characteristics and comparable half-lives, which can be applied in diagnostics. H4picoopa was designed and executed as a high-denticity ligand for chelation of [225Ac]Ac3+, and the complexation characteristics have been explored through nuclear magnetic resonance (NMR) spectroscopy, solution thermodynamic stability studies, and radiolabeling. The ligand shows highly favorable complexation with La3+ (pM = 17.6), Lu3+ (pM = 21.3), and In3+ (pM = 31.2) and demonstrates effective radiolabeling of both [225Ac]Ac3+ and [111In]In3+ ions achieving quantitative radiochemical conversions (RCCs) under mild conditions (RT, 10 min), accompanied by high serum stability (>97% radiochemical purity (RCP) over 6 days). A bifunctional analogue of H4picoopa was synthesized and conjugated to the Pip-Nle-CycMSHhex peptide for targeting of MC1R positive melanoma tumors. In vivo single-photon emission computed tomography (SPECT) and biodistribution studies of the 111In-radiolabeled bioconjugate in mice bearing B16-F10 tumors showed good radiotracer stability, although improved tumor targeting could not be achieved for imaging purposes. This work highlights H4picoopa as a very promising platform for application of [225Ac]Ac3+ and [111In]In3+ as a theranostic pair and allows great versatility for the incorporation of other directing vectors. The logical synthetic approach reported here for bifunctional H4picoopa, involving an azide-functionalized covalent linker and CuI-catalyzed alkyne-azide cycloaddition, allows for ease of optimization of bioconjugate pharmacokinetics and will be valuable for further radiopharmaceutical applications moving forward.