The site of Dimona South is a knapping locality in the Negev desert of Israel, situated at the raw material source. A test excavation followed by a salvage excavation conducted during 2020 revealed a partly buried archaeological layer that was exposed over an area of ~ 40 m2 and yielded a well-preserved lithic assemblage. Optically stimulated luminescence (OSL) ages of sediments within and above the archaeological layer fall within MIS 5. With a lithic assemblage dominated by Nubian Levallois technology, this site provides a rare opportunity for analysis of a well-dated, in situ Nubian assemblage. A refitting study in combination with an attribute analysis of the whole assemblage allowed the reconstruction of the Nubian reduction sequence. Our analyses indicate that a specifically pre-planned Nubian point production system existed at the site. It is characterized by the early preparation of an acute distal ridge and its careful maintenance throughout the reduction process until the cores were exhausted. These characteristics stand out from most Levantine Middle Paleolithic assemblages.The discovery of Dimona South allows us to revisit some of the technological issues at the heart of debates about Levallois Nubian technology that could not be addressed from analyses of partial surface assemblages. These new data from a secure and dated context are crucial to the inter-site and regional technological comparisons, informing our views of the Nubian technology and its role in the Middle Paleolithic world of eastern Africa, Arabia and the Levant.