Ns Duration Research Articles

Ablation of polyimide thin-film on carrier glass using 355 nm and 37 ns laser pulses

Ablation of the polyimide (PI) thin-film deposited on glass substrate was investigated theoretically and experimentally to find out the important characteristics during the laser-lift off (LLO) process for the fabrication of flexible electronics. Instead of using conventional ablation induced by the front irradiation on the specimen, the LLO process employs the backside irradiation on the specimen transferred through the glass substrate, assuming that the ablation occurs volumetrically in the narrow region inside the PI film near the PI and glass interface. Experimentally the PI film on glass was exposed to a pulse laser beam emitted from UV laser of 355 nm in wavelength and 37 ns in duration. Depths and morphologies of the ablation spots were observed by the surface profiler and the FE-SEM. Theoretically the ablation process was estimated by the photothermal mechanism, using the one-dimensional heat conduction equation with volumetric absorption of the laser beam. It was shown that the experimental ablation could be described through the theoretical analysis based on the surface and the bulk photothermal models. While the former assumed that the ablation occurred only on the ablation front surface, the latter assumed the ablation was accompanied by the thermal and volumetric bond breakage. In general, the theoretical calculations agreed well with the experimental observations. In particular, the experimental observations of the ablation for the backside irradiation case that was the core phenomenon in the LLO process was explained appropriately by the features predicted using the bulk model.

587 nm nanosecond optical pulse generation by synchronously-driven gain-switched laser diodes with optical injection locking

We describe a novel optical pulse source that can generate yellow-orange optical pulses based on the synchronization of two gain-switched laser diodes under continuous-wave laser light injection. Sum frequency generation of amplified 1309 nm and 1064 nm optical pulses produced 587 nm pulses with 1 ns duration and 2 nJ pulse energy at a repetition rate of 5 MHz. The presented optical pulse source can also produce bursts of picosecond optical pulses inside nanosecond pulse envelopes. These optical pulses are applicable to stimulated emission depletion microscopy for super-resolution imaging of biomedical specimens with green- or yellow- fluorescent proteins labels.

Evaluation of four injection profiles for uniform contrast-enhanced signal intensity profiles in MR angiography.

Gadolinium concentration variation during acquisition of contrast-enhanced MR angiography (CE-MRA) may lead to artifacts. To compare signal intensity (SI) profiles of four different contrast agent injection strategies during CE-MRA with the goal of minimizing SI variation during acquisition. Prospective. Forty subjects randomized to receive one of four injection profiles of gadobenate dimeglumine (0.1 mmol/kg), either undiluted (0.5 M) or diluted to 40 ml total volume. Tested profiles: 1) nondiluted single-phase ("standard" NS; 1.6 ml/s), 2) diluted single-phase (DS; 1.6 ml/s), 3) diluted biphasic (DB; 9 ml @ 3.3 ml/s, 29 ml @ 1.4 ml/s), 4) patient-tailored protocol using linear prediction (DT). Time-resolved SI measured at 3T with spoiled gradient echo sequences having analogous parameters to those of CE-MRA. Plateau arrival time, rise time, duration, peak and tail SI, plateau quality (sum of squared residuals; SSR), average SI for each injection type derived were used. Two-tailed t-test. Peak SI, arrival, and rise times were not significantly different between groups, excepting peak SI DB slightly > DS (P = 0.042). Duration of NS vs. the diluted groups was significantly shorter (all P < 0.0001), and DS duration was significantly shorter than that of DT and DB (NS 11.4 ± 3.5 vs. DS 22.9 ± 4.3, DB 25.4 ± 2.3, DT 28.3 ± 4.1 sec). Quality (SSR) of the 20-second plateau was significantly better for DS, DB, DT as compared with NS (all P < 0.001). Three different strategies to power-inject diluted gadobenate dimeglumine targeting a 20-second plateau produced SI profiles with longer duration, more consistent plateau, and no significant loss in peak SI. Such injection profiles may provide more uniform SI during CE-MRA, potentially reducing blurring artifacts. 2 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2019;50:1808-1816.

Miniature High-Power Nanosecond Laser Diode Transmitters Using the Simplest Possible Avalanche Drivers

The state-of-the-art long-distance near-infrared optical radars use laser-diode-based miniature pulsed transmitters producing optical pulses of 3–10 ns in duration and peak power typically below 40 W. The duration of the transmitted optical pulses becomes a bottleneck in the task of improving the radar ranging precision, particularly due to the progress made in developing single photon avalanche detectors. The speed of miniature high-current drivers is limited by the speed of the semiconductor switch, either a gallium nitride field-effect transistor, the most popular alternative nowadays, or a silicon avalanche bipolar junction transistor (ABJT), which was traditional in the past. Recent progress in the physical understanding of peculiar 3-D transients promises further enhancement in speed and efficiency of properly modified ABJTs, but that is not the only factor limiting the transmitter speed. We show here that a low-inductance miniature transmitter assembly containing only a specially developed capacitor, a more advanced transistor chip than that used in commercial ABJTs and a laser diode, has allowed peak power from 40 to 180 W to be reached in optical pulses of 1–2 ns in duration without after-pulsing relaxation oscillations. This finding is of interest for compact low-cost, long-distance decimeter-precision lidars, particularly for automotive applications.

Experimental study of pulsed microwave discharges at pressures ranging over five orders of magnitude

Microwave discharge igniter (MDI) is a discharge system developed to initiate combustion in automotive engines. The MDI uses a sequence of N = 700 microwave (2.45 GHz) pulses 100 ns in duration separated by 1 μs. The initial breakdown is provided by the first microwave pulse, 5 μs in duration. The aim of pulsing the microwave signal is to keep an optimal combination of parameters when, even at elevated pressures, (i) the discharge propagates over the largest possible volume; (ii) the plasma is non-equilibrium. Properties of plasma produced by MDI igniter in non-combustible gas mixtures at ambient gas temperature and gas pressure in the range between 0.2 mbar and 8 bar were studied experimentally. Discharge spatial structure was analyzed with the help of time-resolved ICCD imaging. Near-UV optical emission spectra taken in different pulses provided the information about rotational and vibrational temperatures. The electric field was estimated on the basis of ratio of emission of the second positive and the first negative systems of molecular nitrogen.

Experimental study of fast electron generation in intense short duration laser solid interaction at grazing incidence

We report a study on the generation of relativistic fast electrons during the interaction of an ultrashort (45 fs) laser pulse with a variety of targets (Al, Cu, Mo, Si, polyvinyl chloride, and Perspex), at a nearly grazing angle of incidence (∼70°) at an intensity of ∼1.2 × 1018 W/cm2. The fast electron emission direction was found to be dependent on the laser pulse amplified spontaneous emission (ASE) contrast, its extent, and laser pulse duration. For a 45 fs laser pulse and an ASE extent of 1 ns, the electron emission direction changes from a nearly target surface (∼7° ± 2°, divergence: ∼30°) to a specular direction (∼20° ± 2°, divergence: ∼20°) when the contrast was decreased from ∼10−7 to ∼10−6. It further shifts toward target normal with an increase in the ASE duration from 1 ns to 5 ns. For an ASE contrast of ∼10−7 and ∼10−6 with a 1 ns duration, preplasma density scale lengths of ∼30 μm and ∼45 μm were estimated, respectively, from the HELIOS hydrodynamic code. For a longer ASE duration in the range of 1–5 ns, the density scale length was in the range of ∼45–140 μm. Further, for a 45 fs pulse and a 1 ns ASE level, the electron beam charge was found to be higher in the case of Mo (∼52 pC) and Si (∼122 pC) targets compared to the other targets (∼23–34 pC). A further increase in the charge of the electron beam of ∼1.45× (∼75 pC) and ∼2.7× (∼141 pC) was also observed with Mo for a longer pulse duration of ∼170 fs (ASE 1 ns) and a larger ASE duration of 5 ns at (45 fs pulse), respectively. The electron beam properties were found to be independent of laser polarization (p, s, and circular) except a slightly higher charge (∼1.5×) was observed in the case of p-polarized laser light. The fast electron spectrum showed a maximum energy of 1 MeV with electron temperatures of ∼390 keV and ∼320 keV for the target surface and specular direction, respectively. Electron acceleration and their emission are explained in terms of applicable mechanisms in preformed plasma on the target surface considering the role of surface fields which is also established by the 2D particle in cell simulation.

Electric field induced second harmonic (E-FISH) generation for characterization of fast ionization wave discharges at moderate and low pressures

The electric field in an ionization wave discharge in nitrogen at 20–100 mbar, initiated by positive polarity, high-voltage, ns duration pulses, is measured by ps second harmonic generation. The axial electric field component is determined both during the propagation of the ionization wave along the discharge tube, and after the wave reaches the grounded electrode, spanning the entire discharge gap. The temporal resolution of the present measurements is 200 ps, with the spatial resolution in the axial direction of approximately 0.5 mm. The second harmonic signal exhibits a quadratic dependence on the Laplacian electric field but indicates that in this pressure range most of the signal is generated within the wall of the tube. Absolute calibration of the signal is obtained from the current shunt data, after the ionization wave has reached the grounded electrode. Comparison of the data taken at different pressures shows that the peak value of the axial electric field in the wave front, 8–11 kV cm−1, has a fairly weak dependence on pressure, with the peak reduced electric field reaching ≈2000 Td at 20 mbar. Reducing the pressure from 100 to 20 mbar, while keeping the discharge pulse voltage waveform the same, steepens the ionization wave front considerably, from 3.0 to 1.0 ns full width at half maximum. The results demonstrate that ps second harmonic generation may be employed for electric field measurements in low-pressure discharges, discharges sustained in small diameter capillary tubes, and discharges sustained in gas mixtures with low nonlinear susceptibility, at the conditions when the detection of the signal generated directly in the plasma is challenging. High temporal resolution of the present measurements indicates a possibility of detection of non-local electron kinetics effects induced by a rapidly varying, high peak value electric field in low and moderate pressure ionization wave discharges.

Measurement of Laser-Induced Plasma: Stark Broadening Parameters of Pb(II) 2203.5 and 4386.5 Å Spectral Lines.

In this work, the Stark broadening parameters (widths and shifts) of the 2203.5 Å and 4386.5 Å Pb(II) spectral lines have been investigated and measured in laser-induced breakdown spectroscopy (LIBS), using a lead sample (99.999% purity). A Q-switched neodymium-doped yttrium aluminum garnet (Nd:YAG) laser operating at its fundamental wavelength (10 640 Å), generating pulses of 290 mJ, 7 ns of duration, and a repeat frequency of 20 Hz, has been used for the ablation of said lead sample in vacuum and in a controlled argon atmosphere. A study to understand the expansion dynamics of the lead produced plasma was performed. The spectra have been obtained and measured at different time delays of the plasma evolution in the range of 0.15-9 μs, at which the temperature and electron number density are in the ranges of 28 200-8000 K and 1.3 × 1017 to 3 × 1015 cm-3, respectively. A graphical representation of the evolution of temperature and electron number density versus 0.3 to 6.5 μs delay from the laser pulse is presented. The important effect of the different environment where the plasma expands has been pointed out. Local thermodynamic equilibrium conditions have been checked. The obtained results of the Stark widths and shifts at the different temperatures and densities of electrons have been compared with the limited data available in the literature. This study aims to obtain more accurate values for these parameters and also to establish regularities and similarities for said parameters.

Stimulated backscatter of laser light from BigFoot hohlraums on the National Ignition Facility

The high implosion velocity, high adiabat BigFoot design [Casey et al., Phys. Plasmas 25, 056308 (2018)] has produced the highest neutron yield to date in an ignition hohlraum on the National Ignition Facility. It has used up to 500 TW of peak power and nearly 2 MJ of laser energy in pulses up to 8 ns in duration, with the goal of fielding controlled implosions with high coupled energy, which can suppress deleterious hydrodynamic instabilities. However, when the laser pulse exceeds 6 ns with the laser energy greater than 1.6 MJ, stimulated Brillouin scattering (SBS) reaches levels that may damage optical components in the laser. Pending development of techniques to reduce SBS, limitation of laser power, and energy to avoid damage prevents the full exploitation of this approach to ignition. In this manuscript, we present three-dimensional simulations that match the experimentally measured SBS energy, in particular, reproducing quantitatively the time in the pulse when maximum backscatter occurs, and its magnitude across ∼10 BigFoot experiments. The demonstrated robustness of the modeling, which combines LASNEX and pF3D simulations, motivates us to explore and recommend several feasible SBS mitigation strategies: modified laser pointing, different laser frequencies for each cone of beams, increased laser bandwidth on all or some of the cones, and materials with a mixture of light and heavy atoms lining the inside of the hohlraum walls.

Human aquaporin 4 gating dynamics under axially oriented electric-field impulses: A non-equilibrium molecular-dynamics study.

Human aquaporin 4 has been studied using non-equilibrium molecular dynamics simulations in the absence and presence of pulses of external electric fields. The pulses were 100 ns in duration and 0.005-0.015 V/Å in intensity acting along the pores' axes. Water diffusivity and the dipolar response of various residues of interest within the pores have been studied. Results show relatively little change in levels of water permeability per se within aquaporin channels during axially oriented field impulses, although care must be taken with regard to statistical certainty. However, the spatial variation of water permeability vis-à-vis electric-field intensity within the milieu of the channels, as revealed by heterogeneity in diffusivity-map gradients, indicates the possibility of somewhat enhanced diffusivity, owing to several residues being affected substantially by external fields, particularly for HIS 201 and 95 and ILE 93. This has the effect of increasing slightly intra-pore water diffusivity in the "pore-mouths" locale, albeit rendering it more spatially uniform overall vis-à-vis zero-field conditions (via manipulation of the selectivity filter).

Dissipative Soliton Resonances in a Mode-Locked Holmium-Doped Fiber Laser

We demonstrate what is believed to be the first dissipative-soliton-resonance mode-locking of a holmium-doped fiber (HDF) laser. A telecommunication band fiber source was used to pump a piece of thulium-doped fiber, resulting in 1.9-μm band lasing, which was subsequently used to intra-cavity-pump another piece of HDF, resulting in lasing beyond 2 μm. A nonlinear optical-loop mirror incorporating two pieces of high nonlinear fiber (HNLF) was employed as an artificial saturable absorber, and, meanwhile, to enhance the cavity nonlinearity. By varying the pump power from ~2.1 to ~4 W, the produced pulse could be tuned from ~4.85 to ~20.8 ns in duration while maintaining a nearly constant peak power, i.e., peak power clamping (PPC) effect. By further incorporating different lengths of HNLF, it was found that the longer the HNLF, the larger the pulse duration and the lower the pulse peak power, due to the expected stronger PPC effect. Meanwhile, spectral blue-shifting effect has occurred due to the increased fiber attenuation loss. Based on our preliminary experiments using the HDF laser as a test bed, a higher pulse energy at a longer wavelength could be expected with further enhanced pumping and gain-tailoring designs.

Time-resolved and multiple-angle Thomson scattering on gas-puff Z-Pinch plasmas at pinch time.

A 526.5 nm Thomson scattering diagnostic laser enables probing of the plasma conditions of neon gas-puff z-pinch implosions with temporal resolution. Splitting the laser into two 2.5 J pulses, both 2.3 ns in duration and separated by 4 ns, allows observation of sub-nanosecond time-resolved spectra for a total time of 7 ns. Collection optics were set at 90° and 30° to the laser, observing the same on-axis scattering volume with a radial extent of 0.4 mm. The spectra from both angles were collected by using the same streak camera, using a coupling system that allowed us to obtain temporal, spectral, and angular resolution in the same image. By comparing the ion-acoustic spectra from the two angles, we determined electron temperature and a range of possible electron densities. Measurements made in the 1-3 ns period before pinch time show best fit (determined by a least-squares method) electron densities of around 2 × 1019 cm-3, increasing to 1.5 × 1020 cm-3 in the 3 ns following the start of the x-ray burst (t = 0 ns) from the pinch. The electron temperature increases from 300 eV to 500 eV at t = 0 ns before decreasing to below 300 eV after pinch time. With the present parameters (probe beam, collection angles, and electron temperature and density), this diagnostic method is too insensitive to electron density to provide more than a constraint on that parameter. Plasma regimes in which this technique could determine electron density with some precision are calculated.

OA055] Preliminary test of innovative strip silicon detectors for therapeutic proton beam monitoring

Purpose Unlike currently used gas ionization chambers, solid state detectors offer large granularity and sensitivity to single incoming particles, therefore being ideally suited to improve the present technology for beam monitoring in particle therapy. However, several drawbacks, such as radiation damage, prevented their use so far on high flux therapeutic beams. Two prototype devices for monitoring particle beams are under development, based on innovative silicon low-gain avalanche detectors optimized for time resolution (Ultra Fast Silicon Detectors – UFSDs). Preliminary results with proton beams are presented. Methods UFSDs are low-gain avalanche detectors optimized for time resolution, where sensors as thin as 50 μ m provide signals of ∼ 1 ns time duration with time resolutions of tenths of ps, and large enough signal-to-noise ratio to efficiently discriminate proton signal. One prototype device is being developed to directly count individual protons at high rates, while a second one is under investigation to measure the beam energy with time-of-flight techniques. This requires the design of custom UFSD sensors as well VLSI readout electronics. From simulations’ results and first beam tests with UFSD pads, strip detectors were produced, with two geometries (30 mm and 15 mm length) and different doping modalities to improve radiation hardness. In parallel, prototypes of a new readout chip have been submitted to the foundry. Results Strips sensors were characterized in the laboratory through laser test and I(V) curve studies, and a test with a therapeutic proton beam, with energy ranging from 62 to 227 MeV, was done. Results were obtained via offline analysis of the collected waveforms for Boron and Gallium doped sensors. Time resolution of 35 ps, signal duration of ns, and good S/N separation were found. A gain degradation of 20% was founded in single pad sensors after 10 12 protons/cm2 irradiation. Pile-up effects are under investigation. Conclusions Based on the preliminary results, UFSDs are found to be a promising improvement of monitor chamber. The aim of this contribution is to review the advancement of the project and to report on the results of the test of UFSD strip sensors with a therapeutic proton beam.

Ultrafast dynamic response of single-crystal β-HMX (octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine)

We report experimental and computational studies of shock wave dynamics in single-crystal β-HMX on an ultrafast time scale. Here, a laser-based compression drive (∼1 ns in duration; stresses of up to ∼40 GPa) is used to propagate shock waves normal to the (110) and (010) lattice planes. Ultrafast time-domain interferometry measurements reveal distinct, time-dependent relationships between the shock wave velocity and particle velocity for each crystal orientation, which suggest evolving physical processes on a sub-nanosecond time scale. To help interpret the experimental data, elastic shock wave response was simulated using a finite-strain model of crystal thermoelasticity. At early propagation times (&amp;lt;500 ps), the model is in agreement with the data, which indicates that the mechanical response is dominated by thermoelastic deformation. The model agreement depends on the inclusion of nonlinear elastic effects in both the spherical and deviatoric stress-strain responses. This is achieved by employing an equation-of-state and a pressure-dependent stiffness tensor, which was computed via atomistic simulation. At later times (&amp;gt;500 ps), the crystal samples exhibit signatures of inelastic deformation, structural phase transformation, or chemical reaction, depending on the direction of wave propagation.

Effects of nanosecond pulsed electric fields (nsPEFs) on the human fungal pathogen Candida albicans: an in vitro study

Candida albicans is the leading human fungal pathogen that causes many life-threatening infections. Notably, the current clinical trial data indicate that Candida species shows the emerging resistance to anti-fungal drugs. The aim of this study was to evaluate the antifungal effects of nanosecond pulsed electric fields (nsPEFs) as a novel drug-free strategy in vitro. In this study, we investigated the inactivation and permeabilization effects of C. albicans under different nsPEFs exposure conditions (100 pulses, 100 ns in duration, intensities of 20, 40 kV cm−1). Cell death was studied by annexin-V and propidium iodide staining. The changes of intracellular Ca2+ concentration after nsPEFs treatment were observed using Fluo-4 AM. Results show that C. albicans cells and biofilms were both obviously inhibited and destroyed after nsPEFs treatment. Furthermore, C. albicans cells were significantly permeabilized after nsPEFs treatment. Additionally, nsPEFs exposure led to a large amount of DNA and protein leakage. Importantly, nsPEFs induced a field strength-dependent apoptosis in C. albicans cells. Further experiments revealed that Ca2+ involved in nsPEFs induced C. albicans apoptosis. In conclusion, this proof-of-concept study provides a potential alternative drug-free strategy for killing pathogenic Candida species.

Ultraviolet Pulsed Laser-Induced Fluorescence Nonlinearity in Optically Thick Organic Samples.

A simple two-component model is worked out to investigate pulsed laser-induced fluorescence in complex organic samples, like biological tissues, optically thick at the excitation wavelength. Expression for emitted fluorescence signal is obtained. Saturation process is shown to be determined not only by fluorophores excited by the laser, but non-fluorescent chromophores with overlapping absorption band as well. For homogeneous samples the forms of saturation curves are determined by fluorophore's features. Experimental saturation curves of bulk paper and mice tissues ultraviolet pulsed laser-induced fluorescence are discussed considering this model. For the ns and shorter laser pulse durations with wavelength in 200-300nm region, pulse energy density should be less than 200μJ/cm2 for correct quantitative comparison of fluorescence spectra of biological tissues with primarily tryptophan fluorescence.

Experimental demonstration of a terahertz extended interaction oscillator driven by a pseudospark-sourced sheet electron beam

We have recently proposed to combine the advantages of a pseudospark-sourced sheet electron beam (PS-SEB) with a planar slow wave structure to generate high power terahertz radiation. To verify this idea, experimental investigation of an extended interaction oscillator based on the PS-SEB has been conducted and presented. A PS-SEB of approximately 1.0 mm × 0.17 mm in size with 21.5 A peak current (1.26 × 104 A/cm2 beam current density) and 34.5 kV peak voltage was measured after propagating a distance of 10-mm without the need of an external focusing magnetic field. A radiation pulse of ∼35 ns in duration and an output power of over 10 W at a frequency of ∼0.2 THz were measured.

Quantitative analysis of vacuum-ultraviolet radiation from nanosecond laser-zinc interaction

The paper reports measurements of the vacuum-ultraviolet spectral irradiances of a flat zinc target over a wavelength region of 124–164 nm generated by 10 and 60 ns duration low-intensities, 5×109-3×1010 W cm−2, 1.06 μm wavelength laser pulses. Maximum radiation conversion efficiencies of 2.5%/2πsr and 0.8%/2πsr were measured for 60 and 10 ns laser pulses at the intensities of 5×109 and 1.4×1010 W cm−2, respectively. Atomic structure calculations using a relativistic configuration-interaction, flexible atomic code and a developed non-local thermodynamic equilibrium population kinetics model in comparison to the experimental spectra detected by the Seya-Namioka type monochromator reveal the strong broadband experimental emission originates mainly from 3d94p-3d94s, 3d94d-3d94p and 3d84p-3d84s, 3d84d-3d84p unresolved-transition arrays of double and triple ionized zinc, respectively. Two-dimensional radiation-hydrodynamics code is used to investigate time-space plasma evolution and spectral radiation of a 10 ns full-width-at-half-maximum Gaussian laser pulse-zinc interaction.

Development of a 100 J, 10 Hz laser for compression experiments at the High Energy Density instrument at the European XFEL

In this paper we review the design and development of a 100 J, 10 Hz nanosecond pulsed laser, codenamed DiPOLE100X, being built at the Central Laser Facility (CLF). This 1 kW average power diode-pumped solid-state laser (DPSSL) is based on a master oscillator power amplifier (MOPA) design, which includes two cryogenic gas cooled amplifier stages based on DiPOLE multi-slab ceramic Yb:YAG amplifier technology developed at the CLF. The laser will produce pulses between 2 and 15 ns in duration with precise, arbitrarily selectable shapes, at pulse repetition rates up to 10 Hz, allowing real-time shape optimization for compression experiments. Once completed, the laser will be delivered to the European X-ray Free Electron Laser (XFEL) facility in Germany as a UK-funded contribution in kind, where it will be used to study extreme states of matter at the High Energy Density (HED) instrument.

Ursolic acid and carvacrol may be potential inhibitors of dormancy protein small heat shock protein16.3 of Mycobacterium tuberculosis

Small heat shock protein16.3 (sHSP16.3) is a crucial protein for survival of Mycobacterium tuberculosis (MTB) in its host. Besides, this protein acts as a molecular chaperone during stress and is indispensable for MTB’s growth, virulence and cell-wall thickening. sHSP16.3 is also a promising candidate for vaccine, serodiagnosis and drug design as well. In the present study, we have targeted sHSP16.3 with two phytochemicals, namely ursolic acid and carvacrol using in silico approach. Molecular docking analysis showed that both phytochemicals (ursolic acid and carvacrol) have docked with sHSP16.3 and shown tendency to inhibit the function of this vital protein of MTB. In addition, both compounds have exhibited strong compatibility with sHSP16.3 during whole 60 ns duration of molecular dynamics simulation. Further, the molecular mechanic/generalized Born/Poisson–Boltzmann surface area (MM/G/P/BSA) free energies were calculated which showed that both phytocompounds have stable and favourable binding energies causing strong binding with binding site of sHSP16.3. Taking together, the data of present study suggest that both phytocompounds may be potential inhibitor of sHSP16.3 of MTB and a best alternative to standard anti-tuberculosis drugs.