Argania spinosa (L.) Skeels is an endemic species to Morocco that has multiple uses. It plays multiple important roles in terms of its botanical, ecological, and economic properties. However, the domestication of this species will open up considerable economic opportunities for Morocco. Here, for the first time, we assessed the effect of different doses of compost and NPK fertilizers on the vegetative growth parameters, biochemical and antioxidant potential of the Argania spinosa plant, and soil properties. Over a two-year period (2022-2023), eight different treatments were applied across two experimental sites. These treatments included the following: T0 (Control), T1 (F1-80.50.70 g NPK/plant), T2 (F1-125.75.100 g NPK/plant), T3 (F2-160.100.140 g NPK/plant), T4 (F2-250.150.200 g NPK/plant), T5 (F1-2.5 kg/plant compost), T6 (F1-5 kg/plant compost), T7 (F2-5 kg/plant compost), and T8 (F2-10 kg/plant compost), with F1 and F2 being the frequencies of application. We compared several doses of fertilizers with no fertilization as a control. The results showed a significant influence of the compost and NPK fertilizer on the vegetative growth parameters. For the Tamjlojt site, the first year is important because treatments T3 and T4 significantly increased height by 71.94 ± 21.15% and 74.31 ± 12.31%, respectively. For the circumference, the results showed a significant improvement by the treatments T4 and T3, and T1 demonstrated the highest gain. For the collar diameter, all treatments showed a significant difference. The most notable difference was observed with treatments T3 and T7 with 115.63 ± 33.88% and 101.09 ± 20.84%, respectively. For the Rasmouka site, the second year was the most important. The treatments with the most important height increase were T7 and T8, with a value of 43.14 ± 10.06% and 36.44 ± 9.95%; the same was observed for collar diameter as a significant increase was found in T8 and T7 with a value of 55.05 ± 15.7% and 54.08 ± 9.64%. For the circumference parameter, the treatments that increased significantly this parameter were T8 and T7 with a value of 53.36 ± 15.11% and 50.34 ± 11.29% in 2023. In addition, the highest content of carbohydrates was recorded for the treatment T3 with a value of 148.89 ± 8.11 (mg EG/g). For phenolic determination, the highest value was 2532 ± 457.13 (µg GAE/mL), shown for treatment T1. For flavonoids, the treatments that showed a significant effect were T1 and T6 with a value of 2261.98 ± 184.61 and 1237.70 ± 95.65 (µg QE/mL), respectively. For the impact on soil properties, the electrical conductivity, at the Tamjlojt site, treatment T1 showed a significant increase to 1139.00 ± 241.30 (ms/cm), while at the Rasmouka site, treatment T8 showed a significant increase to 303.33 ± 9.33 (ms/cm). Concerning organic carbon, all treatments resulted in increased percentages of this parameter in the soil. For the Tamjlojt site, the T7 treatment had a significant positive effect on this parameter with a value of 0.87 ± 0.12%. For the Rasmouka site, the T3 treatment increased the percentage of organic carbon with a value of 1.17 ± 0.07%. In addition, the organic matter content showed an improvement with a value of 2.02 ± 0.12%. As there are no previous studies in Argania spinosa fertilization, this study greatly contributes to our understanding of the benefits of using different fertilizers at different doses, in particular T8 and T7 as organic fertilizers and T3, T4 as chemical ones, on argan growth, the biochemical and antioxidant properties of leaves, and its soil properties.