A field experiment was conducted at Research Farm of Bihar Agricultural University, Sabour, Bhagalpur, Bihar, during season of 2019-20, to find out the effect of optimization of nitrogen doses for high yield potential of wheat with plant growth regulators. The experiment was laid out in randomized block and replicated thrice. The experiment ten treatment of different doses of fertilizer with plant growth regulators chlormequat chloride and tebuconazole applied at 0.2% and 0.1% respectively. Experimental revealed that application of 150% recommended doses of NPK resulted significantly higher available nitrogen (171.82 kg ha-1), available phosphorus (27.01 kg ha-1) and available potassium (192.27 kg ha-1) in soil after the harvest of wheat significantly highest nitrogen, phosphorus and potassium uptake by wheat recorded with 150% RDF of NPK with chlormequat chloride (0.2%) and tebuconazole (0.1%). Significantly highest grain and straw yield recorded with 150% NPK + chlormequat chloride (0.2%) and tebuconazole (0.1%) at par with 100% RDF as compare to other treatments harvest index was recorded 125% RDF of N and chlormequat chloride (0.2%) and tebuconazole (0.1%) and 150% RDF of N and chlormequat chloride (0.2%) and tebuconazole (0.1%). The uptake of key nutrients, nitrogen, phosphorus and potassium followed a similar pattern, with 150% recommended doses of NPK along chlormequat chloride (0.2%) and tebuconazole (0.1%). optimal NPK application resulted in positive nutrient balance in the soil. This investigation that managing nutrient with growth regulator in wheat is an economically viable and ecologically sound approach to improve both crop production and soil health through balanced nutrition.