The present study held to investigate the relationship between azurophilic granules in the peripheral blood leukocytes and bovine theileriosis. A total number of 100 adult cattle of different sexes were subjected to this study. Whole blood, blood and lymph smears were collected from each animal under study. Theileria annulata was confirmed by both conventional and molecular methods. The obtained results revealed that the percentages of interaerythrocytic stage (Signet Ring), azurophilic granules and Koch’s blue bodies in the samples of the examined cattle were 28%, 42% and 39%, respectively. Evaluation of the reliability of azurophilic granules occurrence in peripheral blood leukocytes as novel method for diagnosis of tropical theileriosis revealed that the occurrence of these granules should be considered as an easy, inexpensive and rapid method for diagnosis of bovine theileriosis.